RESEARCH PROGRESS OF CELL-SCAFFOLD COMPLEX IN TENDON TISSUE ENGINEERING_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

ZHU Ying , LI Min.

College of Life Sciences, Fujian Normal University, Fuzhou Fujian, 350108, P.R.China. Corresponding author: LI Min, E-mail: mli@fjnu.edu.cn;

Corresponding author：

Keywords：

Tendon tissue engineering; Seed cells; Scaffold material; Mechanical stimulation

DOI：

10.7507/1002-1892.20130109

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Objective To review the research progress of cell-scaffold complex in the tendon tissue engineering. Methods Recent literature concerning cell-scaffold complex in the tendon tissue engineering was reviewed, the research situation of the cell-scaffold complex was elaborated in the aspects of seed cells, scaffolds, cell culture, and application. Results In tendon tissue engineering, a cell-scaffold complex is built by appropriate seed cells and engineered scaffolds. Experiments showed that modified seed cells had better therapeutic effects. Further, scaffold functionality could be improved through surface modification, growth factor cure, mechanical stimulation, and contact guidance. Among these methods, mechanical stimulation revealed the most significant results in promoting cell proliferation and function. Through a variety of defect models, it is demonstrated that the use of cell-scaffold complex could achieve satisfactory results for tendon regeneration. Conclusion The cell-scaffold complex for tendon tissue engineering is a popular research topic. Although it has not yet met the requirement of clinical use, it has broad application prospects.

Citation： ZHU Ying,LI Min.. RESEARCH PROGRESS OF CELL-SCAFFOLD COMPLEX IN TENDON TISSUE ENGINEERING. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(4): 481-485. doi: 10.7507/1002-1892.20130109 Copy

1.	5 Chaudhury S, Murphy RJ, Carr AJ. Tendon tissue engineering: the potential application of stem cells, biological factors, and repair scaffolds to improve rotator cuff tendon tears. Tendon Tissue Engineering, 2011, 5: 291-310. 6 司红玉. 组织工程化肌腱修复运动训练致肌腱组织缺损: 细胞材料及其过程中的相关研究. 中国组织工程研究与临床康复, 2010, 14(16): 2975-2978.
2.	7 谭贡霞, 张允蚌. 组织工程化肌腱应用于肌腱运动损伤的修复: 研究与应用现状. 中国组织工程研究与临床康复, 2010, 14(12): 2225-2228. 8 Ma PX. Biomimetic materials for tissue engineering. Adv Drug Deliv Rev, 2008, 60(2): 184-198.
3.	9 张弛, 蒋垚. 纤维连接蛋白表面修饰对促进新型PET材料人工韧带(LARS)细胞黏附的实验研究. 生物骨科材料与临床研究, 2009, 6(5): 16-20. 10 周峰, 袁琳, 梅岩, 等. “接触引导” 和重力场复合作用对 L929 细胞生长取向的影响. 科学通报, 2010, (26): 2582-2586. 11 James R, Kumbar SG, Laurencin CT, et al. Tendon tissue engineering: adipose-derived stem cell and GDF-5 mediated regeneration using electrospun matrix systems. Biomed Mater, 2011, 6(2): 025011. 12 Shen W, Chen X, Chen J, et al. The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration. Biomaterials, 2010, 31(28): 7239-7249. 13 Cooper JA Jr, Bailey LO, Carter JN, et al. Evaluation of the anterior cruciate ligament, medial collateral ligament, achilles tendon and patellar tendon as cell sources for tissue-engineered ligament. Biomaterials, 2006, 27(13): 2747-2754. 14 张文君, 柴殿波, 于建农. 肌腱组织工程的研究进展. 医学综述, 2008, 14(10): 1452-1454.
4.	16 Nixon AJ, Watts AE, Schnabel LV. Cell- and gene-based approaches to tendon regeneration. J Shoulder Elbow Surg, 2012, 21(2): 278-294. 17 Hoffmann A, Pelled G, Turgeman G, et al. Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells. J Clin Invest, 2006, 116(4): 940-952. 18 Lee JY, Zhou Z, Taub PJ, et al. BMP-12 treatment of adult mesenchymal stem cells in vitro augments tendon-like tissue formation and defect repair in vivo. PLoS ONE, 2011, 6(3): e17531. 19 杨志明. 组织工程. 北京: 化学工业出版社, 2002: 250-257.
5.	20 胡康洪, 姚颖. 三维细胞培养技术的研究与应用. 医学分子生物学杂志, 2008, 5(2): 185-188. 21 Herchenhan A, Bayer ML, Svensson RB, et al. In vitro tendon tissue development from human fibroblasts demonstrates collagen fibril diameter growth associated with a rise in mechanical strength. Dev Dyn, 2012, 242(1): 2-8. 22 曲彦隆, 杨志明, 解慧琪, 等. 三维培养状态下肌腱细胞骨架对细胞生物学行为的影响. 中华实验外科杂志, 2004, 21(6): 657-658, i002. 23 詹兴旺, 姜艳, 王文良. 细胞共培养在组织工程研究中的应用及价值. 中国组织工程研究与临床康复, 2011, 15(20): 3759-3762.
6.	26 Yang PJ. Incorporation of protease-sensitive biomaterial degradation and tensile strain for applications in ligament-bone interface tissue engineering. Georgia Institute of Technology, 2012. 27 Nirmalanandhan VS, Rao M, Shearn JT, et al. Effect of scaffold material, construct length and mechanical stimulation on the in vitro stiffness of the engineered tendon construct. J Biomech, 2008, 41(4): 822-828. 28 Deng D, Liu W, Xu F, et al. Engineering human neo-tendon tissue in vitro with human dermal fibroblasts under static mechanical strain. Biomaterials, 2009, 30(35): 6724-6730. 29 Abousleiman R, Reyes Y, McFetridge P, et al. Tendon tissue engineering using cell-seeded umbilical veins cultured in a mechanical stimulator. Tissue Eng Part A, 2009, 15(4): 787-795. 30 Stops AJ, Heraty KB, Browne M, et al. A prediction of cell differentiation and proliferation within a collagen-glycosaminoglycan scaffold subjected to mechanical strain and perfusive fluid flow. J Biomech, 2010, 43(4): 618-626. 31 Nerurkar NL, Sen S, Baker BM, et al. Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds. Acta Biomater, 2011, 7(2): 485-491. 32 秦廷武, 杨志明, 解慧琪, 等. 动态应变下肌腱细胞三维培养的初步研究. 华西医科大学学报, 2002, 33(1): 1-4.
7.	33 Chen JL, Yin Z, Shen WL, et al. Efficacy of hESC-MSCs in knitted silk-collagen scaffold for tendon tissue engineering and their roles. Biomaterials, 2010, 31(36): 9438-9451. 34 Thorfinn J, Angelidis IK, Gigliello L, et al. Bioreactor optimization of tissue engineered rabbit flexor tendons in vivo. J Hand Surg (Eur Vol), 2012, 37(2): 109-114. 35 李宏, 安琦. 肌腱生物反应器的开发与组织工程肌腱的构建. 华东理工大学学报: 自然科学版, 2010, 36(1): 141-145.
8.	36 Saber S, Zhang AY, Ki SH, et al. Flexor tendon tissue engineering: bioreactor cyclic strain increases construct strength. Tissue Eng Part A, 2010, 16(6): 2085-2090. 37 Wang B, Liu W, Zhang Y, et al. Engineering of extensor tendon complex by an ex vivo approach. Biomaterials, 2008, 29(20): 2954-2961. 38 Kuo CK, Marturano JE, Tuan RS. Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs. Sports Med Arthrosc Rehabil Ther Technol, 2010, 2: 20. 39 Kryger GS, Chong AK, Costa M, et al. A comparison of tenocytes and mesenchymal stem cells for use in flexor tendon tissue engineering. J Hand Surg (Am), 2007, 32(5): 597-605.
9.	40 朱肖奇, 秦廷武, 杨志明. 细胞黏附基础与肌腱组织工程. 生物医学工程学杂志, 2004, 21(2): 329-332. 41 Carpenter JE, Hankenson KD. Animal models of tendon and ligament injuries for tissue engineering applications. Biomaterials, 2004, 25(9): 1715-1722.
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35.	李宗虎, 曲彦隆, 周继辉, 等. 肌腱组织工程研究新进展. 中国矫形外科杂志, 2007, 15(18): 1406-1407, 1437.
36.	Chen B, Wang B, Zhang WJ, et al. In vivo tendon engineering with skeletal muscle derived cells in a mouse model. Biomaterials, 2012, 33(26): 6086-6097.
37.	Liu Y, Ramanath HS, Wang DA. Tendon tissue engineering using scaffold enhancing strategies. Trends Biotechnol, 2008, 26(4): 201-209.
38.	吴琳琳. PVA/WSF复合纳米纤维支架材料在肌腱组织工程的初步研究. 福州: 福建师范大学, 2011.
39.	Sheyn D, Mizrahi O, Benjamin S, et al. Genetically modified cells in regenerative medicine and tissue engineering. Adv Drug Deliv Rev, 2010, 62(7-8): 683-698.
40.	王梓. 大鼠骨髓基质干细胞与肌腱细胞在体外间接共培养的实验研究. 大连: 大连医科大学, 2010.
41.	胡春江, 徐宏光. 细胞培养方式与力学刺激. 黑龙江医药科学, 2010, 33(6): 19-21.

1. 5 Chaudhury S, Murphy RJ, Carr AJ. Tendon tissue engineering: the potential application of stem cells, biological factors, and repair scaffolds to improve rotator cuff tendon tears. Tendon Tissue Engineering, 2011, 5: 291-310. 6 司红玉. 组织工程化肌腱修复运动训练致肌腱组织缺损: 细胞材料及其过程中的相关研究. 中国组织工程研究与临床康复, 2010, 14(16): 2975-2978.
2. 7 谭贡霞, 张允蚌. 组织工程化肌腱应用于肌腱运动损伤的修复: 研究与应用现状. 中国组织工程研究与临床康复, 2010, 14(12): 2225-2228. 8 Ma PX. Biomimetic materials for tissue engineering. Adv Drug Deliv Rev, 2008, 60(2): 184-198.
3. 9 张弛, 蒋垚. 纤维连接蛋白表面修饰对促进新型PET材料人工韧带(LARS)细胞黏附的实验研究. 生物骨科材料与临床研究, 2009, 6(5): 16-20. 10 周峰, 袁琳, 梅岩, 等. “接触引导” 和重力场复合作用对 L929 细胞生长取向的影响. 科学通报, 2010, (26): 2582-2586. 11 James R, Kumbar SG, Laurencin CT, et al. Tendon tissue engineering: adipose-derived stem cell and GDF-5 mediated regeneration using electrospun matrix systems. Biomed Mater, 2011, 6(2): 025011. 12 Shen W, Chen X, Chen J, et al. The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration. Biomaterials, 2010, 31(28): 7239-7249. 13 Cooper JA Jr, Bailey LO, Carter JN, et al. Evaluation of the anterior cruciate ligament, medial collateral ligament, achilles tendon and patellar tendon as cell sources for tissue-engineered ligament. Biomaterials, 2006, 27(13): 2747-2754. 14 张文君, 柴殿波, 于建农. 肌腱组织工程的研究进展. 医学综述, 2008, 14(10): 1452-1454.
4. 16 Nixon AJ, Watts AE, Schnabel LV. Cell- and gene-based approaches to tendon regeneration. J Shoulder Elbow Surg, 2012, 21(2): 278-294. 17 Hoffmann A, Pelled G, Turgeman G, et al. Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells. J Clin Invest, 2006, 116(4): 940-952. 18 Lee JY, Zhou Z, Taub PJ, et al. BMP-12 treatment of adult mesenchymal stem cells in vitro augments tendon-like tissue formation and defect repair in vivo. PLoS ONE, 2011, 6(3): e17531. 19 杨志明. 组织工程. 北京: 化学工业出版社, 2002: 250-257.
5. 20 胡康洪, 姚颖. 三维细胞培养技术的研究与应用. 医学分子生物学杂志, 2008, 5(2): 185-188. 21 Herchenhan A, Bayer ML, Svensson RB, et al. In vitro tendon tissue development from human fibroblasts demonstrates collagen fibril diameter growth associated with a rise in mechanical strength. Dev Dyn, 2012, 242(1): 2-8. 22 曲彦隆, 杨志明, 解慧琪, 等. 三维培养状态下肌腱细胞骨架对细胞生物学行为的影响. 中华实验外科杂志, 2004, 21(6): 657-658, i002. 23 詹兴旺, 姜艳, 王文良. 细胞共培养在组织工程研究中的应用及价值. 中国组织工程研究与临床康复, 2011, 15(20): 3759-3762.
6. 26 Yang PJ. Incorporation of protease-sensitive biomaterial degradation and tensile strain for applications in ligament-bone interface tissue engineering. Georgia Institute of Technology, 2012. 27 Nirmalanandhan VS, Rao M, Shearn JT, et al. Effect of scaffold material, construct length and mechanical stimulation on the in vitro stiffness of the engineered tendon construct. J Biomech, 2008, 41(4): 822-828. 28 Deng D, Liu W, Xu F, et al. Engineering human neo-tendon tissue in vitro with human dermal fibroblasts under static mechanical strain. Biomaterials, 2009, 30(35): 6724-6730. 29 Abousleiman R, Reyes Y, McFetridge P, et al. Tendon tissue engineering using cell-seeded umbilical veins cultured in a mechanical stimulator. Tissue Eng Part A, 2009, 15(4): 787-795. 30 Stops AJ, Heraty KB, Browne M, et al. A prediction of cell differentiation and proliferation within a collagen-glycosaminoglycan scaffold subjected to mechanical strain and perfusive fluid flow. J Biomech, 2010, 43(4): 618-626. 31 Nerurkar NL, Sen S, Baker BM, et al. Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds. Acta Biomater, 2011, 7(2): 485-491. 32 秦廷武, 杨志明, 解慧琪, 等. 动态应变下肌腱细胞三维培养的初步研究. 华西医科大学学报, 2002, 33(1): 1-4.
7. 33 Chen JL, Yin Z, Shen WL, et al. Efficacy of hESC-MSCs in knitted silk-collagen scaffold for tendon tissue engineering and their roles. Biomaterials, 2010, 31(36): 9438-9451. 34 Thorfinn J, Angelidis IK, Gigliello L, et al. Bioreactor optimization of tissue engineered rabbit flexor tendons in vivo. J Hand Surg (Eur Vol), 2012, 37(2): 109-114. 35 李宏, 安琦. 肌腱生物反应器的开发与组织工程肌腱的构建. 华东理工大学学报: 自然科学版, 2010, 36(1): 141-145.
8. 36 Saber S, Zhang AY, Ki SH, et al. Flexor tendon tissue engineering: bioreactor cyclic strain increases construct strength. Tissue Eng Part A, 2010, 16(6): 2085-2090. 37 Wang B, Liu W, Zhang Y, et al. Engineering of extensor tendon complex by an ex vivo approach. Biomaterials, 2008, 29(20): 2954-2961. 38 Kuo CK, Marturano JE, Tuan RS. Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs. Sports Med Arthrosc Rehabil Ther Technol, 2010, 2: 20. 39 Kryger GS, Chong AK, Costa M, et al. A comparison of tenocytes and mesenchymal stem cells for use in flexor tendon tissue engineering. J Hand Surg (Am), 2007, 32(5): 597-605.
9. 40 朱肖奇, 秦廷武, 杨志明. 细胞黏附基础与肌腱组织工程. 生物医学工程学杂志, 2004, 21(2): 329-332. 41 Carpenter JE, Hankenson KD. Animal models of tendon and ligament injuries for tissue engineering applications. Biomaterials, 2004, 25(9): 1715-1722.
10.
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14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
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35. 李宗虎, 曲彦隆, 周继辉, 等. 肌腱组织工程研究新进展. 中国矫形外科杂志, 2007, 15(18): 1406-1407, 1437.
36. Chen B, Wang B, Zhang WJ, et al. In vivo tendon engineering with skeletal muscle derived cells in a mouse model. Biomaterials, 2012, 33(26): 6086-6097.
37. Liu Y, Ramanath HS, Wang DA. Tendon tissue engineering using scaffold enhancing strategies. Trends Biotechnol, 2008, 26(4): 201-209.
38. 吴琳琳. PVA/WSF复合纳米纤维支架材料在肌腱组织工程的初步研究. 福州: 福建师范大学, 2011.
39. Sheyn D, Mizrahi O, Benjamin S, et al. Genetically modified cells in regenerative medicine and tissue engineering. Adv Drug Deliv Rev, 2010, 62(7-8): 683-698.
40. 王梓. 大鼠骨髓基质干细胞与肌腱细胞在体外间接共培养的实验研究. 大连: 大连医科大学, 2010.
41. 胡春江, 徐宏光. 细胞培养方式与力学刺激. 黑龙江医药科学, 2010, 33(6): 19-21.

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