EFFECT OF HUMAN UMBILICAL CORD BLOOD ON CONTENT OF TRACE ELEMENTS IN WHOLE BLOOD DURING FRACTURE HEALING IN RABBITS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LI Yongle ¹ , HE Qiang ² , FAN Xiandong ² , LIU Baoping ² , LI Tiejun ² , SUN Yizhong ²

1College of Basic Medicine, Hebei North University, Zhangjiakou Hebei, 075000, P.R.China;;
2Department of Orthopaedics, No.251 Hospital of Chinese PLA. Corresponding author: HE Qiang, E-mail: 1755758534@qq.com;

Corresponding author：

Keywords：

Umbilical cord blood; Tibial fracture; Trace element Whole blood; Rabbit

DOI：

10.7507/1002-1892.20130150

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Objective To study the effect of the human umbilical cord blood on the content of trace elements in whole blood during fracture healing in rabbits and to explore the mechanism of promoting fracture healing. Methods The right tibial fracture model was made in 63 white New Zealand rabbits (aged, 4-5 months; weighing, 2.0-2.5 kg). The fracture site was treated with 3 mL human umbilical cord blood (group A, n=21) and 3 mL normal saline (group B, n=21) at 3 and 8 days after operation, and was not treated as a control (group C, n=21). At 1, 2, 3, 4, 5, and 6 weeks after operation, the X-ray and histological observations were done; the contents of zinc, copper, magnesium, ferrum, calcium, and phosphorus were detected. Results X-ray observation showed that the fracture healing speed of group A was significantly faster than that of groups B and C; the fracture healing X-ray score of group A was significantly higher than that of groups B and C at 2-6 weeks (P lt; 0.05). The histological observation indicated that new trabeculae and osteoid of group A were significantly more than those of groups B and C; at 2-5 weeks, the histological score of group A was significantly higher than that of groups B and C (P lt; 0.05); at 6 weeks, the score of group A was significantly higher than that of group B (P lt; 0.05), but no significant difference was found between groups A and C (P gt; 0.05). Changes trend of the trace elements in 3 groups after operation was basically consistent. The content of copper first decreased and then gradually increased; the contents of ferrum, zinc, and magnesium at different time points decreased, but were basically stable; the content of calcium first increased and then decreased; the content of phosphorus first decreased and then increased. The contents of copper, zinc, magnesium, ferrum, calcium, and phosphorus in group A were significantly higher than those in groups B and C at different time points (P lt; 0.05), but there was no significant difference between groups B and C (P gt; 0.05). Conclusion Injection of the human umbilical cord blood at the fracture end of rabbits can significantly slow down the loss of trace elements in whole blood, ensure the contents of necessary trace elements during fracture healing, which may be one of the mechanisms of the umbilical cord blood promoting fracture healing.

Citation： LI Yongle,HE Qiang,FAN Xiandong,LIU Baoping,LI Tiejun,SUN Yizhong. EFFECT OF HUMAN UMBILICAL CORD BLOOD ON CONTENT OF TRACE ELEMENTS IN WHOLE BLOOD DURING FRACTURE HEALING IN RABBITS. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(6): 673-679. doi: 10.7507/1002-1892.20130150 Copy

1.	孙之镐, 刘向前, 祁开泽, 等. 接骨紫金丹对家兔骨折愈合期间几种金属元素的影响. 中国骨伤, 1999, 12(6): 16-18.
2.	张会生, 何强, 李达, 等. 脐血对骨折愈合影响的实验研究. 中国骨伤, 2001, 14(11): 673-674.
3.	孙保勇, 陈文直, 贾小林, 等. 低强度脉冲超声促进骨折愈合的动物实验研究. 中国矫形外科杂志, 2005, 13(6): 443-446, 452.
4.	Huo MH, Troiano NW, Pelker RR, et al. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. Orthop Res, 1991, 9(3): 383-390.
5.	王杨, 李秀群, 黄云, 等. 不同方法制备生物衍生骨体内植入的组织学观察. 中国修复重建外科杂志, 2007, 21(9): 1003-1006.
6.	翟照, 陈凤苞. 家兔自体骨髓移植对骨痂中钙镁铜含量的影响. 中国骨伤, 2002, 15(2): 82-83.
7.	吴从俊, 袁本祥, 熊敦善. 骨愈注射液对家兔骨折愈合影响的实验研究. 中国中医骨伤科杂志, 2002, 10(6): 10-13.
8.	王智兴, 胡美珠. 骨折患者的血清微量元素变化. 中华骨科杂志, 1993, 13(1): 58-59.
9.	Simşek A, Senköylü A, Cila E, et al. Is there a correlation between severity of trauma and serum trace element levels. Acta Orthop Traumatol Turc, 2006, 40(2): 140-143.
10.	檀少强. 中医药促进骨折愈合的机理研究与应用. 云南中医中药杂志, 2011, 32(8): 85-87.
11.	Lazer T, Paz-Tal O, Katz O, et al. Trace elements’ concentrations in maternal and umbilical cord plasma at term gestation: a comparison between active labor and elective cesarean delivery. Matern Fetal Neonatal Med, 2012, 25(3): 286-289.
12.	李小薇, 关怀, 朴丰源, 等. 大连地区母血及脐血中21种微量元素浓度测定分析. 中国优生与遗传杂志, 2009, 17(4): 55-57.
13.	尹霞, 郑玲玲, 白岩. 刺老苞根皮干预大鼠骨折模型矿物元素含量变化. 广东微量元素科学, 2011, 18(6): 23-27.
14.	Igarashi A, Yamaguchi M. Great increase in bone 66 kDa protein and osteocalcin at later stages with healing rat fractures: effect of zinc treatment. Int J Mol Med, 2003, 11(2): 223-228.
15.	赵益峰, 王海滨, 孟纯阳, 等. 节段性骨缺损修复过程中几种微量元素含量的研究. 实用骨科杂志, 2010, 16(2): 113-117.
16.	Ryder KM, Shorr RI, Bush AJ, et al. Magnesium intake from food and supplements is associated with bone mineral density in healthy older whitesubjects. J Am Geriatri Soci, 2005, 53(11): 1875-1880.
17.	张奋耿, 凌秋英, 叶泽亮, 等. 伤科接骨片对实验性家兔骨折模型血清微量元素含量的影响. 中医正骨, 2008, 20(5): 9-10.
18.	金惠铭, 王建枝. 病理生理学. 北京: 人民卫生出版社, 2004: 44.
19.	彭明, 王玉州, 李伟, 等. 胎儿脐血对骨折愈合作用的实验研究. 铁道医学, 1996, 24(3): 134-135.
20.	王广宇, 赵芳, 朱旅云. 脐血中免疫细胞的免疫学特性概述. 医学综述, 2010, 16(3): 343-345.
21.	张丽. 脐血浆活性成分分析及其对脐血CD34+细胞增殖的影响. 郑州: 郑州大学, 2011.
22.	Qu Z, Guo L, Fang G, et al. Biological characteristics and effect of human umbilical cord mesenchymal stem cells (hUCMSCs) grafting with blood plasma on bone regeneration in rats. Cell Biochem Biophys, 2012, 63(2): 171-181.

1. 孙之镐, 刘向前, 祁开泽, 等. 接骨紫金丹对家兔骨折愈合期间几种金属元素的影响. 中国骨伤, 1999, 12(6): 16-18.
2. 张会生, 何强, 李达, 等. 脐血对骨折愈合影响的实验研究. 中国骨伤, 2001, 14(11): 673-674.
3. 孙保勇, 陈文直, 贾小林, 等. 低强度脉冲超声促进骨折愈合的动物实验研究. 中国矫形外科杂志, 2005, 13(6): 443-446, 452.
4. Huo MH, Troiano NW, Pelker RR, et al. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. Orthop Res, 1991, 9(3): 383-390.
5. 王杨, 李秀群, 黄云, 等. 不同方法制备生物衍生骨体内植入的组织学观察. 中国修复重建外科杂志, 2007, 21(9): 1003-1006.
6. 翟照, 陈凤苞. 家兔自体骨髓移植对骨痂中钙镁铜含量的影响. 中国骨伤, 2002, 15(2): 82-83.
7. 吴从俊, 袁本祥, 熊敦善. 骨愈注射液对家兔骨折愈合影响的实验研究. 中国中医骨伤科杂志, 2002, 10(6): 10-13.
8. 王智兴, 胡美珠. 骨折患者的血清微量元素变化. 中华骨科杂志, 1993, 13(1): 58-59.
9. Simşek A, Senköylü A, Cila E, et al. Is there a correlation between severity of trauma and serum trace element levels. Acta Orthop Traumatol Turc, 2006, 40(2): 140-143.
10. 檀少强. 中医药促进骨折愈合的机理研究与应用. 云南中医中药杂志, 2011, 32(8): 85-87.
11. Lazer T, Paz-Tal O, Katz O, et al. Trace elements’ concentrations in maternal and umbilical cord plasma at term gestation: a comparison between active labor and elective cesarean delivery. Matern Fetal Neonatal Med, 2012, 25(3): 286-289.
12. 李小薇, 关怀, 朴丰源, 等. 大连地区母血及脐血中21种微量元素浓度测定分析. 中国优生与遗传杂志, 2009, 17(4): 55-57.
13. 尹霞, 郑玲玲, 白岩. 刺老苞根皮干预大鼠骨折模型矿物元素含量变化. 广东微量元素科学, 2011, 18(6): 23-27.
14. Igarashi A, Yamaguchi M. Great increase in bone 66 kDa protein and osteocalcin at later stages with healing rat fractures: effect of zinc treatment. Int J Mol Med, 2003, 11(2): 223-228.
15. 赵益峰, 王海滨, 孟纯阳, 等. 节段性骨缺损修复过程中几种微量元素含量的研究. 实用骨科杂志, 2010, 16(2): 113-117.
16. Ryder KM, Shorr RI, Bush AJ, et al. Magnesium intake from food and supplements is associated with bone mineral density in healthy older whitesubjects. J Am Geriatri Soci, 2005, 53(11): 1875-1880.
17. 张奋耿, 凌秋英, 叶泽亮, 等. 伤科接骨片对实验性家兔骨折模型血清微量元素含量的影响. 中医正骨, 2008, 20(5): 9-10.
18. 金惠铭, 王建枝. 病理生理学. 北京: 人民卫生出版社, 2004: 44.
19. 彭明, 王玉州, 李伟, 等. 胎儿脐血对骨折愈合作用的实验研究. 铁道医学, 1996, 24(3): 134-135.
20. 王广宇, 赵芳, 朱旅云. 脐血中免疫细胞的免疫学特性概述. 医学综述, 2010, 16(3): 343-345.
21. 张丽. 脐血浆活性成分分析及其对脐血CD34+细胞增殖的影响. 郑州: 郑州大学, 2011.
22. Qu Z, Guo L, Fang G, et al. Biological characteristics and effect of human umbilical cord mesenchymal stem cells (hUCMSCs) grafting with blood plasma on bone regeneration in rats. Cell Biochem Biophys, 2012, 63(2): 171-181.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF HUMAN UMBILICAL CORD BLOOD ON CONTENT OF TRACE ELEMENTS IN WHOLE BLOOD DURING FRACTURE HEALING IN RABBITS

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