EFFECT OF LANTHANUM CHLORIDE ON CYTOKINES EXPRESSION OF RAW264.7 INDUCED BY ALUMINA CERAMIC PARTICLES_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

NIETao ,  DAIMin , JIANGChuan , LIUXiang

Department of Orthopaedics, the First Affiliated Hospital of Nanchang University, Nanchang Jiangxi, 330006, P. R. China;

Corresponding author：

DAIMin, Email: daimin@medmail.com.cn

Keywords：

Lanthanum chloride; Alumina ceramic particle; Interleukin 1β; Tumor necrosis factor α; Nuclear factor κB

DOI：

10.7507/1002-1892.20140209

Video：

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Objective To investigate the relationship between alumina ceramic particles and aseptic loosening of the joint prosthesis and the effect of lanthanum chloride on the secretion of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) of macrophage RAW264.7 induced by alumina ceramic particles. Methods RAW264.7 cells were cultured in vitro and divided randomly into 4 groups according to different culture solutions:blank control group (group A),1 mg/mL alumina ceramic particles (group B),1 mg/mL alumina ceramic particles and 10 μmol/L lanthanum chloride (group C),and 10 μmol/L lanthanum chloride (group D).The cell growth was detected by MTT,and ELISA,RT-PCR,and Western blot were used to test the expressions of IL-1β,TNF-α,and nuclear factor κB (NF-κB). Results There was no significant difference in cell growth among all groups by MTT (F=2.180,P=0.142).RT-PCR results showed that the expressions of IL-1β,TNF-α,and NF-κB mRNA in group B were significantly higher than those in the other 3 groups (P<0.05); the expressions in group D were significantly lower than those in group A (P<0.05).ELISA results showed that the contents of IL-1β and TNF-α in group B were significantly higher than those in the other 3 groups (P<0.05); the contents in group D were significantly lower than those in group A (P<0.05).Western blot analysis revealed that the expression of NF-κB protein in group B was significantly higher than that in the other 3 groups (P<0.05). Conclusion Alumina ceramic particles can stimulate the secretion of IL-1β and TNF-α of macrophage,and lanthanum chloride can inhibit the secretion of IL-1β and TNF-α of macrophage.

Citation： NIETao, DAIMin, JIANGChuan, LIUXiang. EFFECT OF LANTHANUM CHLORIDE ON CYTOKINES EXPRESSION OF RAW264.7 INDUCED BY ALUMINA CERAMIC PARTICLES. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(8): 951-954. doi: 10.7507/1002-1892.20140209 Copy

1.	Guo F,Lou Y,Feng N,et al.Exposure to lanthanum compound diminishes LPS-induced inflammation-associated gene expression:involvements of PKC and NF-kappaB signaling pathways.Biometals,2012,23(4):669-680.
2.	Hussein SZ,Yusoff KM,Makpol S,et al.Gelam honey inhibits the production of proinflammatory,Mediators NO,PGE(2),TNF-α,and IL-6 in carrageenan-induced acute paw edema in rats.Evid Based Complement Alternat Med,2012,2012:109636.
3.	王海,宋科官.磨损颗粒诱导假体周围骨溶解的机制及基因治疗现状.哈尔滨医科大学学报,2011,45(1):95-98.
4.	戴闽,程明,刘虎诚,等.不同浓度金属磨损颗粒对破骨细胞体外分化的影响.中国矫形外科杂志,2011,46(4):316-319.
5.	Kanaji A,Caicedo MS,Virdi AS,et al.Co-Cr-Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes:a role for osteocytes in particle-induced inflammation.Bone,2009,45(3):528-533.
6.	Hamadouche M,Boutin P,Daussange J,et al.Alumina-on-alumina total hip arthroplasty:a minimum 18.5 year follow-up study.J Bone Joint Surg (Am),2002,84-A(1):69-77.
7.	帅浪,戴闽,郝亮,等.陶瓷磨损颗粒对体外培养外周血单核细胞分泌TNF-α的影响.生物骨科材料与临床研究,2006,3(2):7-9.
8.	Lee SS,Sharma AR,Choi BS,et al.The effect of TNFalpha secreted from macrophages activated by titanium particles on osteogenic activity regulated by WNT/BMP signaling in osteoprogenitor cells.Biomaterials,2012,33(17):4251-4263.
9.	Cuetara BL,Crotti TN,O'Donoghue AJ,et al.Cloning and characterization of osteoclast precursors from the RAW264.7 cell line.In Vitro Cell Dev Biol Anim,2006,42(7):182-188.
10.	戴闽,程明,张斌,等.RAW264.7细胞在体外分化成破骨细胞的研究.天津医药,2010,38(7):596-598.
11.	Hutchison AJ,Gill M,Copley JB,et al.Lanthanum carbonate versus placebo for management of hyperphosphatemia in patients undergoing peritoneal dialysis:a subgroup analysis of a phase 2 randomized controlled study of dialysis patients.BMC Nephrol,2013,14:40.
12.	Yamaguchi M.Role of nutritional zinc in the prevention of osteoporosis.Mol Cell Biochem,2010,338(1-2):241-254.
13.	Clohisy JC,Hirayama T,Frazier E,et al.NF-kB signaling blockade abolishes implant particle-induced osteoclastogenesis.J Orthop Res,2004,22(1):13-20.
14.	Ren W,Li XH,Chen BD,et al.Erythromycin inhibits wear debris-induced osteoclastogenesis by modulation of murine macrophage NF-kappaB activity.J Orthop Res,2004,22(1):21-29.
15.	Goater JJ,O'Keefe RJ,Rosier RN,et al.Efficacy of ex vivo OPG gene therapy in preventing wear debris induced osteolysis.J Orthop Res,2002,20(2):169-173.

1. Guo F,Lou Y,Feng N,et al.Exposure to lanthanum compound diminishes LPS-induced inflammation-associated gene expression:involvements of PKC and NF-kappaB signaling pathways.Biometals,2012,23(4):669-680.
2. Hussein SZ,Yusoff KM,Makpol S,et al.Gelam honey inhibits the production of proinflammatory,Mediators NO,PGE(2),TNF-α,and IL-6 in carrageenan-induced acute paw edema in rats.Evid Based Complement Alternat Med,2012,2012:109636.
3. 王海,宋科官.磨损颗粒诱导假体周围骨溶解的机制及基因治疗现状.哈尔滨医科大学学报,2011,45(1):95-98.
4. 戴闽,程明,刘虎诚,等.不同浓度金属磨损颗粒对破骨细胞体外分化的影响.中国矫形外科杂志,2011,46(4):316-319.
5. Kanaji A,Caicedo MS,Virdi AS,et al.Co-Cr-Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes:a role for osteocytes in particle-induced inflammation.Bone,2009,45(3):528-533.
6. Hamadouche M,Boutin P,Daussange J,et al.Alumina-on-alumina total hip arthroplasty:a minimum 18.5 year follow-up study.J Bone Joint Surg (Am),2002,84-A(1):69-77.
7. 帅浪,戴闽,郝亮,等.陶瓷磨损颗粒对体外培养外周血单核细胞分泌TNF-α的影响.生物骨科材料与临床研究,2006,3(2):7-9.
8. Lee SS,Sharma AR,Choi BS,et al.The effect of TNFalpha secreted from macrophages activated by titanium particles on osteogenic activity regulated by WNT/BMP signaling in osteoprogenitor cells.Biomaterials,2012,33(17):4251-4263.
9. Cuetara BL,Crotti TN,O'Donoghue AJ,et al.Cloning and characterization of osteoclast precursors from the RAW264.7 cell line.In Vitro Cell Dev Biol Anim,2006,42(7):182-188.
10. 戴闽,程明,张斌,等.RAW264.7细胞在体外分化成破骨细胞的研究.天津医药,2010,38(7):596-598.
11. Hutchison AJ,Gill M,Copley JB,et al.Lanthanum carbonate versus placebo for management of hyperphosphatemia in patients undergoing peritoneal dialysis:a subgroup analysis of a phase 2 randomized controlled study of dialysis patients.BMC Nephrol,2013,14:40.
12. Yamaguchi M.Role of nutritional zinc in the prevention of osteoporosis.Mol Cell Biochem,2010,338(1-2):241-254.
13. Clohisy JC,Hirayama T,Frazier E,et al.NF-kB signaling blockade abolishes implant particle-induced osteoclastogenesis.J Orthop Res,2004,22(1):13-20.
14. Ren W,Li XH,Chen BD,et al.Erythromycin inhibits wear debris-induced osteoclastogenesis by modulation of murine macrophage NF-kappaB activity.J Orthop Res,2004,22(1):21-29.
15. Goater JJ,O'Keefe RJ,Rosier RN,et al.Efficacy of ex vivo OPG gene therapy in preventing wear debris induced osteolysis.J Orthop Res,2002,20(2):169-173.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF LANTHANUM CHLORIDE ON CYTOKINES EXPRESSION OF RAW264.7 INDUCED BY ALUMINA CERAMIC PARTICLES

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