HISTOLOGICAL OBSERVATION AND VASCULARIZATION EVALUATION OF PORCINE ACELLULAR DERMAL MATRIX PROCESSED WITH MATRIX METALLOPROTEINASE 7 AFTER IMPLANTED INTO RATS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANGFang ¹ , WUYan ² , SHIWen ¹ , SHAOYang ¹ , MAYindong ¹ , JIAJun ¹ , XINNaijun ¹ , WUQiuhe ¹ ,  SONGGuodong ¹

1. Department of Burns, Jinan Central Hospital Affiliated to Shandong University, Jinan Shandong, 250013, P. R. China;
2. Medical Image Center, Jinan Central Hospital Affiliated to Shandong University, Jinan Shandong, 250013, P. R. China;

Corresponding author：

SONGGuodong, Email: sgd.zxyy@163.com

Keywords：

Porcine acellular dermal matrix; Matrix metalloproteinase 7; Vascularization; Fibroblasts Rat

DOI：

10.7507/1002-1892.20140251

Video：

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Objective To investigate the histological changes and vascularization of the porcine acellular dermal matrix (P-ADM) processed with matrix metalloproteinase 7 (MMP-7) (P-ADM-pm) after implanted into rats. Methods Sixty-two pieces of porcine reticular layer dermis which were from the pig abdominal skin and obtained by using a mechanical method, were randomly divided into group A (n=31) and group B (n=31). The porcine reticular layer dermis in 2 groups were treated with decellularization (P-ADM), then the P-ADM in group B were treated with processing by MMP-7 (P-ADM-pm). Thirty adult male Wistar rats were selected. P-ADM (group A) and P-ADM-pm (group B) were subcutaneously transplanted into the left and right fascia lacuna, respectively. The implants were harvested from 6 rats at 3, 7, 14, 21, and 28 days after implantation, respectively. Gross, histochemical, and immunohistochemical observations, and scanning electron microscopy (SEM) examination were performed to observe host cells, microvessels infiltration and histological changes in the implants. Results No rat died in the experiment, incision healed well and no obvious inflammatory reaction was seen in all rats. Gross observation suggested that the implants of 2 groups were encapsulated by a thin layer of connective tissue at 7 days after implantation. With the time of implantation, the microvessels increased and coarsened, and the changes of group B were more obvious than those of group A. At 21 days, the microvessels of 2 groups decreased, and the implants of group B showed complete vascularization. The histochemical and immunohistochemical observations showed that group A had more severe inflammatory response than group B. Fibroblasts and microvessels in group B appeared in the superficial zone of implant at 3 and 7 days after implantation and they could be observed in the center zone of implant at 14 and 21 days. However, fibroblasts and microvessels in group A appeared in the superficial zone of implant at 3 and 14 days and they could not be observed in the center zone of implant at 28 days. Fibroblasts and microvessels of group B were significantly more than those of group A (P < 0.05). SEM examination showed that more fibroblasts and new collagen fibrils were observed in group B at 14 days. Conclusion The host response to P-ADM-pm is similar to normal wound healing, and P-ADM-pm as implantable scaffold material plays a good template conduction role.

Citation： WANGFang, WUYan, SHIWen, SHAOYang, MAYindong, JIAJun, XINNaijun, WUQiuhe, SONGGuodong. HISTOLOGICAL OBSERVATION AND VASCULARIZATION EVALUATION OF PORCINE ACELLULAR DERMAL MATRIX PROCESSED WITH MATRIX METALLOPROTEINASE 7 AFTER IMPLANTED INTO RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(9): 1161-1167. doi: 10.7507/1002-1892.20140251 Copy

1.	Wainwright DJ.Use of an acellular allograft dermal matrix (AlloDerm) in the management of full-thickness burns.Burns, 1995, 21(4):243-248.
2.	Lattari V, Jones LM, Varcelotti JR, et al.The use of a permanent dermal allograft in full-thickness burns of the hand and foot:a report of three cases.J Burn Care Rehabil, 1997, 18(2):147-155.
3.	Chung KH, Kim TK, Cho BC, et al.Surgical treatment of aplasia cutis congenita with acellular dermal graft and cultured epithelial autograft.Dermatol Surg, 2009, 35(3):546-549.
4.	Eo S, Cho S, Shin H, et al.The utility of Alloderm in hand resurfacing.J Plast Reconstr Aesthet Surg, 2010, 63(1):e41-43.
5.	Kinsella CR Jr, Grunwaldt LJ, Cooper GM, et al.Scalp reconstruction:regeneration with acellular dermal matrix.J Craniofac Surg, 2010, 21(2):605-607.
6.	Shaikh FM, Giri SK, Durrani S, et al.Experience with porcine acellular dermal collagen implant in one-stage tension-free reconstruction of acute and chronic abdominal wall defects.World J Surg, 2007, 31(10):1966-1972.
7.	Chavarriaga LF, Lin E, Losken A, et al.Management of complex abdominal wall defects using acellular porcine dermal collagen.Am Surg, 2010, 76(1):96-100.
8.	冯祥生, 潘银根, 谭家驹, 等.异种(猪)脱细胞真皮与自体表皮复合移植研究.中华整形外科杂志, 2000, 16(1):40-42.
9.	陈炯, 韩春茂, 张力成.异种(猪)脱细胞真皮基质与自体皮复合移植的临床应用.中华整形外科杂志, 2002, 18(5):271-272.
10.	齐顺贞, 李辉, 孙志刚, 等.不同种属脱细胞真皮基质与自体刃厚皮复合移植近期效果的对比研究.中国修复重建外科杂志, 2005, 19(1):61-63.
11.	Richter GT, Smith JE, Spencer HJ, et al.Histological comparison of implanted cadaveric and porcine dermal matrix grafts.Otolaryngol Head Neck Surg, 2007, 137(2):239-242.
12.	Broderick G, Mclnryre J, Noury M, et al.Dermal collagen matrices for ventral hernia repair:comparative analysis in a rat model.Hernia, 2012, 16(3):333-343.
13.	Ngo MD, Aberman HM, Hawes ML, et al.Evaluation of human acellular dermis versus porcine acellular dermis in an in vivo model for incisional henia repair.Cell Tissue Bank, 2011, 12(2):135-145.
14.	Campbell KT, Burns NK, Rios CN, et al.Human versus non-cross-linked porcine acellular dermal matrix used for ventral hernia repair:comparison of in vivo fibrovascular remodeling and mechanical repair strength.Plast Reconstr Surg, 2011, 127(6):2321-2332.
15.	Prasertsung I, Kanokpanont S, Bunaprasert T, et al.Development of acellular dermis from porcine skin using periodic pressurized technique.J Biomed Mater Res B Appl Biomater, 2008, 85(1):210-219.
16.	DeSagun EZ, Botts JL, Srivastava A, et al.Long-term outcome of xenogenic dermal matrix implantation in immunocompetent rats.J Surg Res, 2001, 96(1):96-106.
17.	Butler CE.The role of bioprosthetics in abdominal wall reconstruction.Clin Plast Surg, 2006, 33(2):199-211.
18.	Burns NK, Jaffari MV, Rios CN, et al.Non-cross-linked porcine acellular dermal matrices for abdominal wall reconstruction.Plast Reconstr Surg, 2010, 125(1):167-176.
19.	Thrailkill KM, Clay Bunn R, Fowlkes JL.Matrix metalloproteinases:their potential role in the pathogenesis of diabetic nephropathy.Endocrine, 2009, 35(1):1-10.
20.	Wilson CL, Matrisian LM.Matrilysin:an epithelial matrix metalloproteinase with potentially novel functions.Int J Biochem Cell Biol, 1996, 28(2):123-136.
21.	张爱军, 宋国栋, 曹成波, 等.猪胶原基真皮支架成纤维细胞生长特性的观察.山东大学学报:医学版, 2007, 45(7):718-721.
22.	姜金珠, 武岩, 鹿清, 等.基质金属蛋白酶对猪脱细胞真皮基质免疫原性的影响.山东大学学报:医学版, 2011, 49(8):74-78.
23.	邵阳, 武岩, 贾军, 等.低免疫原性猪真皮支架制备及其细胞相容性评价.中华烧伤杂志, 2012, 28(5):329-335.
24.	Wong AK, Schonmeyer B, Singh P, et al.Histologic analysis of angiogenesis and lymphangiogenesis in acellular human dermis.Plast Reconstr Surg, 2008, 121(4):1144-1152.
25.	Song G, Nguyen DT, Pietramaggiori G, et al.Use of the parabiotic model in studies of cutaneous wound healing to define the participation of circulating cells.Wound Repair Regen, 2010, 18(4):426-432.
26.	Salem AK, Steven R, Pearson RG, et al.Interactions of 3T3 fibroblasts and endothelial cells with defined pore features.J Biomed Mater Res, 2002, 61(2):212-217.

1. Wainwright DJ.Use of an acellular allograft dermal matrix (AlloDerm) in the management of full-thickness burns.Burns, 1995, 21(4):243-248.
2. Lattari V, Jones LM, Varcelotti JR, et al.The use of a permanent dermal allograft in full-thickness burns of the hand and foot:a report of three cases.J Burn Care Rehabil, 1997, 18(2):147-155.
3. Chung KH, Kim TK, Cho BC, et al.Surgical treatment of aplasia cutis congenita with acellular dermal graft and cultured epithelial autograft.Dermatol Surg, 2009, 35(3):546-549.
4. Eo S, Cho S, Shin H, et al.The utility of Alloderm in hand resurfacing.J Plast Reconstr Aesthet Surg, 2010, 63(1):e41-43.
5. Kinsella CR Jr, Grunwaldt LJ, Cooper GM, et al.Scalp reconstruction:regeneration with acellular dermal matrix.J Craniofac Surg, 2010, 21(2):605-607.
6. Shaikh FM, Giri SK, Durrani S, et al.Experience with porcine acellular dermal collagen implant in one-stage tension-free reconstruction of acute and chronic abdominal wall defects.World J Surg, 2007, 31(10):1966-1972.
7. Chavarriaga LF, Lin E, Losken A, et al.Management of complex abdominal wall defects using acellular porcine dermal collagen.Am Surg, 2010, 76(1):96-100.
8. 冯祥生, 潘银根, 谭家驹, 等.异种(猪)脱细胞真皮与自体表皮复合移植研究.中华整形外科杂志, 2000, 16(1):40-42.
9. 陈炯, 韩春茂, 张力成.异种(猪)脱细胞真皮基质与自体皮复合移植的临床应用.中华整形外科杂志, 2002, 18(5):271-272.
10. 齐顺贞, 李辉, 孙志刚, 等.不同种属脱细胞真皮基质与自体刃厚皮复合移植近期效果的对比研究.中国修复重建外科杂志, 2005, 19(1):61-63.
11. Richter GT, Smith JE, Spencer HJ, et al.Histological comparison of implanted cadaveric and porcine dermal matrix grafts.Otolaryngol Head Neck Surg, 2007, 137(2):239-242.
12. Broderick G, Mclnryre J, Noury M, et al.Dermal collagen matrices for ventral hernia repair:comparative analysis in a rat model.Hernia, 2012, 16(3):333-343.
13. Ngo MD, Aberman HM, Hawes ML, et al.Evaluation of human acellular dermis versus porcine acellular dermis in an in vivo model for incisional henia repair.Cell Tissue Bank, 2011, 12(2):135-145.
14. Campbell KT, Burns NK, Rios CN, et al.Human versus non-cross-linked porcine acellular dermal matrix used for ventral hernia repair:comparison of in vivo fibrovascular remodeling and mechanical repair strength.Plast Reconstr Surg, 2011, 127(6):2321-2332.
15. Prasertsung I, Kanokpanont S, Bunaprasert T, et al.Development of acellular dermis from porcine skin using periodic pressurized technique.J Biomed Mater Res B Appl Biomater, 2008, 85(1):210-219.
16. DeSagun EZ, Botts JL, Srivastava A, et al.Long-term outcome of xenogenic dermal matrix implantation in immunocompetent rats.J Surg Res, 2001, 96(1):96-106.
17. Butler CE.The role of bioprosthetics in abdominal wall reconstruction.Clin Plast Surg, 2006, 33(2):199-211.
18. Burns NK, Jaffari MV, Rios CN, et al.Non-cross-linked porcine acellular dermal matrices for abdominal wall reconstruction.Plast Reconstr Surg, 2010, 125(1):167-176.
19. Thrailkill KM, Clay Bunn R, Fowlkes JL.Matrix metalloproteinases:their potential role in the pathogenesis of diabetic nephropathy.Endocrine, 2009, 35(1):1-10.
20. Wilson CL, Matrisian LM.Matrilysin:an epithelial matrix metalloproteinase with potentially novel functions.Int J Biochem Cell Biol, 1996, 28(2):123-136.
21. 张爱军, 宋国栋, 曹成波, 等.猪胶原基真皮支架成纤维细胞生长特性的观察.山东大学学报:医学版, 2007, 45(7):718-721.
22. 姜金珠, 武岩, 鹿清, 等.基质金属蛋白酶对猪脱细胞真皮基质免疫原性的影响.山东大学学报:医学版, 2011, 49(8):74-78.
23. 邵阳, 武岩, 贾军, 等.低免疫原性猪真皮支架制备及其细胞相容性评价.中华烧伤杂志, 2012, 28(5):329-335.
24. Wong AK, Schonmeyer B, Singh P, et al.Histologic analysis of angiogenesis and lymphangiogenesis in acellular human dermis.Plast Reconstr Surg, 2008, 121(4):1144-1152.
25. Song G, Nguyen DT, Pietramaggiori G, et al.Use of the parabiotic model in studies of cutaneous wound healing to define the participation of circulating cells.Wound Repair Regen, 2010, 18(4):426-432.
26. Salem AK, Steven R, Pearson RG, et al.Interactions of 3T3 fibroblasts and endothelial cells with defined pore features.J Biomed Mater Res, 2002, 61(2):212-217.

Chinese Journal of Reparative and Reconstructive Surgery

HISTOLOGICAL OBSERVATION AND VASCULARIZATION EVALUATION OF PORCINE ACELLULAR DERMAL MATRIX PROCESSED WITH MATRIX METALLOPROTEINASE 7 AFTER IMPLANTED INTO RATS

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