EXPRESSIONS OF OSTEOGENESIS RELATED FACTORS IN MG63 CELLS CO-CULTURED WITH DOMESTIC POROUS TANTALUM_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANGQian ^1,2 , ZHANGHui ^1,3 , GENGLixin ⁴ , LIQijia ⁴ , GANHongquan ⁵ , WANGHui ⁶ , BICheng ⁵ ,  WANGZhiqiang ^1,5

1. 唐山市第二医院手一科;
2. Graduate School, Southern Medical University, Guangzhou Guangdong, 510515, P. R. China;
3. Department of Anatomy, Basic Medical College;
4. the First Department of Joint Surgery, 6the First Department of Hand Surgery, Tangshan No. 2 Hospital;
5. Experimental Center, Hebei United University;
6. Department of Orthopaedics, Affiliated Hospital of Hebei United University;

Corresponding author：

WANGZhiqiang, Email: wzhqde@163.com

Keywords：

Domestic porous tantalum; MG63 cells; Runt-related transcri ption factor 2; Osteocalcin; Fibronectin; Bone tissue engineering

DOI：

10.7507/1002-1892.20140306

Video：

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Objective To investigate the feasibil ity of the domestic porous tantalum as scaffold material of bone tissue engineering by observing the expressions of osteogenesis related factors of MG63 cells co-cultured with domestic porous tantalum. Methods MG63 cells were cultured with porous tantalum scaffolds (group A), with porous tantalum leaching solution (group B), and with MEM as control group (group C). The cell adhesion of group A was observed on the scaffolds at 3, 5, and 7 days after culture by scanning electron microscopy (SEM); immunohistochemistry and Western blot methods were used to detect the expressions of Runt-related transcri ption factor 2 (Runx-2), osteocalcin (OC), and fibronectin (FN). Results At 3 days after culture, the cells of group A adhered the surface and pore of the porous tantalum scaffolds, with sparse cell arrangement and less protuberances; at 5 days after culture, adjacent cells connected to be a flat each other, which covered the surface and pore of the scaffold; at 7 days after culture, cells secreted plenty of extracellular matrix, covering most of the material surface. The expressions of Runx-2, OC, and FN were positive in 3 groups; darker staining of the cytoplasm was observed in group A, the expressions were significantly higher in group A than in other 2 groups. The results of immunohistochemistry and Western blot showed that the expressions of Runx-2 and OC were significantly increased in group A when compared with those in groups B and C (P < 0.05), but no significant difference was found between groups B and C (P > 0.05). The expression of FN had no significant difference among 3 groups (P > 0.05). Conclusion Domestic porous tantalum could promote MG63 cells adhesion and growth, and may promote the expressions of Runx-2 and OC, so it can be used as a scaffold material of bone tissue engineering.

Citation： WANGQian, ZHANGHui, GENGLixin, LIQijia, GANHongquan, WANGHui, BICheng, WANGZhiqiang. EXPRESSIONS OF OSTEOGENESIS RELATED FACTORS IN MG63 CELLS CO-CULTURED WITH DOMESTIC POROUS TANTALUM. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(11): 1422-1427. doi: 10.7507/1002-1892.20140306 Copy

1.	Mrosek EH, Schagemann JC, Chung HW, et al. Porous tantalum and poly-epsilon-caprolactone biocomposites for osteochondral defect repair:preliminary studies in rabbits. J Orthop Res, 2010, 28(2):141-148.
2.	朱留洋, 李琪佳.金属钽在骨损伤康复治疗中的应用.中国矫形外科杂志, 2013, 21(6):578-581.
3.	Fernandez-Fairen M, Querales V, Jakowlew A, et al. Tantalum is a good bone graft substitute in tibial tubercle advancement. Clin Orthop Relat Res, 2010, 468(5):1284-1295.
4.	Wang N, Li H, Wang J, et al. Study on the anticorrosion, biocompatibil ity, and osteoin-ductivity of tantalum decorated with tantalum oxide nanotube array films. ACS Appl Mater Interfaces, 2012, 4(9):4516-4523.
5.	甘洪全, 刘鑫, 李琪佳.医学领域的新型骨科植入材料-多孔钽.中国煤炭工业医学杂志, 2014, 17(6):1023-1026.
6.	王大鹏, 李琪佳, 王志强.医用多孔钽的性能及其在膝关节损伤中的应用.中国组织工程研究与临床康复, 2011, 15(29):5463-5466.
7.	甘洪全, 刘鑫, 李琪佳.医用多孔金属钽材料的基础研究与应用.中外医疗, 2014, (14):191-192.
8.	李庆庆, 黄江鸿, 何美健, 等. Runx-2及其在骨组织工程中的应用.国际骨科学杂志, 2014, 35(1):44-46.
9.	张苍宇, 汪新柱, 任广铁, 等.组织工程化骨膜的构建及其在体外的成骨活性检测.中国矫形外科杂志, 2014, 22(3):256-261.
10.	王福科, 刘流, 代晓明, 等.纤维粘连蛋白对部分脱蛋白骨细胞相容性影响研究.昆明医学院学报, 2010, (1):9-14.
11.	耿丽鑫, 甘洪全, 王茜, 等.国产多孔钽对成骨细胞生物相容性及其相关成骨基因表达的影响.第三军医大学学报, 2014, 36(11):1163-1167.
12.	中华人民共和国国家标准. GB/TISO10993-5:2009医疗器械生物学评价.北京:中国标准出版社, 2009.
13.	熊建义, 欧阳建安, 王大平, 等.多孔铌基生物材料的制备及其性能研究.中国临床解剖学杂志, 2013, 31(1):89-94.
14.	马剑, 李乾, 尹芸生.钽金属在医学领域的应用.实用骨科杂志, 2014, 20(4):329-333.
15.	Sagomonyants KB, Hakim-Zargar M, Jhaveri A, et al. Porous tantalum stimulates the prol iferation and osteogenesis of osteoblasts from elderly female patients. J Orthop Res, 2011, 29(4):609-616.
16.	李慕勤, 王健平, 赵莉.天然高分子/羟基磷灰石支架材料对成骨细胞增殖的影响.黑龙江医药科学, 2008, 31(5):1-2.
17.	Fox P, Pogson S, Sutcliffe CJ, et al. Interface interactions between porous titanium/tantalum coatings, produced by Selective Laser Melting (SLM), on a cobalt-chromium alloy. Surface Coatings Techonlogy, 2008, 202(20):5001-5007.
18.	林凤飞, 郑明, 刘伯龄, 等.多孔钽金属与成骨细胞生物相容性的实验研究.中国矫形外科杂志, 2014, 22(12):1119-1123.
19.	赵艳芳, 宋涛, 刘跃亮, 等. RUNX2对BMP9诱导的间充质干细胞C3H10T1/2成骨分化的影响.中国生物工程杂志, 2013, 33(2):21-26.
20.	Hoang QQ, Sicheri F, Howard AJ, et al. Bone recognition mechanism of porcine osteocalcin from crystal structure. Nature, 2003, 425(6961): 977-980.
21.	韩涛, 丁佑铭.骨桥蛋白基本特性及在疾病中的作用的研究进展.实用医药杂志, 2011, 28(6):554-556.

1. Mrosek EH, Schagemann JC, Chung HW, et al. Porous tantalum and poly-epsilon-caprolactone biocomposites for osteochondral defect repair:preliminary studies in rabbits. J Orthop Res, 2010, 28(2):141-148.
2. 朱留洋, 李琪佳.金属钽在骨损伤康复治疗中的应用.中国矫形外科杂志, 2013, 21(6):578-581.
3. Fernandez-Fairen M, Querales V, Jakowlew A, et al. Tantalum is a good bone graft substitute in tibial tubercle advancement. Clin Orthop Relat Res, 2010, 468(5):1284-1295.
4. Wang N, Li H, Wang J, et al. Study on the anticorrosion, biocompatibil ity, and osteoin-ductivity of tantalum decorated with tantalum oxide nanotube array films. ACS Appl Mater Interfaces, 2012, 4(9):4516-4523.
5. 甘洪全, 刘鑫, 李琪佳.医学领域的新型骨科植入材料-多孔钽.中国煤炭工业医学杂志, 2014, 17(6):1023-1026.
6. 王大鹏, 李琪佳, 王志强.医用多孔钽的性能及其在膝关节损伤中的应用.中国组织工程研究与临床康复, 2011, 15(29):5463-5466.
7. 甘洪全, 刘鑫, 李琪佳.医用多孔金属钽材料的基础研究与应用.中外医疗, 2014, (14):191-192.
8. 李庆庆, 黄江鸿, 何美健, 等. Runx-2及其在骨组织工程中的应用.国际骨科学杂志, 2014, 35(1):44-46.
9. 张苍宇, 汪新柱, 任广铁, 等.组织工程化骨膜的构建及其在体外的成骨活性检测.中国矫形外科杂志, 2014, 22(3):256-261.
10. 王福科, 刘流, 代晓明, 等.纤维粘连蛋白对部分脱蛋白骨细胞相容性影响研究.昆明医学院学报, 2010, (1):9-14.
11. 耿丽鑫, 甘洪全, 王茜, 等.国产多孔钽对成骨细胞生物相容性及其相关成骨基因表达的影响.第三军医大学学报, 2014, 36(11):1163-1167.
12. 中华人民共和国国家标准. GB/TISO10993-5:2009医疗器械生物学评价.北京:中国标准出版社, 2009.
13. 熊建义, 欧阳建安, 王大平, 等.多孔铌基生物材料的制备及其性能研究.中国临床解剖学杂志, 2013, 31(1):89-94.
14. 马剑, 李乾, 尹芸生.钽金属在医学领域的应用.实用骨科杂志, 2014, 20(4):329-333.
15. Sagomonyants KB, Hakim-Zargar M, Jhaveri A, et al. Porous tantalum stimulates the prol iferation and osteogenesis of osteoblasts from elderly female patients. J Orthop Res, 2011, 29(4):609-616.
16. 李慕勤, 王健平, 赵莉.天然高分子/羟基磷灰石支架材料对成骨细胞增殖的影响.黑龙江医药科学, 2008, 31(5):1-2.
17. Fox P, Pogson S, Sutcliffe CJ, et al. Interface interactions between porous titanium/tantalum coatings, produced by Selective Laser Melting (SLM), on a cobalt-chromium alloy. Surface Coatings Techonlogy, 2008, 202(20):5001-5007.
18. 林凤飞, 郑明, 刘伯龄, 等.多孔钽金属与成骨细胞生物相容性的实验研究.中国矫形外科杂志, 2014, 22(12):1119-1123.
19. 赵艳芳, 宋涛, 刘跃亮, 等. RUNX2对BMP9诱导的间充质干细胞C3H10T1/2成骨分化的影响.中国生物工程杂志, 2013, 33(2):21-26.
20. Hoang QQ, Sicheri F, Howard AJ, et al. Bone recognition mechanism of porcine osteocalcin from crystal structure. Nature, 2003, 425(6961): 977-980.
21. 韩涛, 丁佑铭.骨桥蛋白基本特性及在疾病中的作用的研究进展.实用医药杂志, 2011, 28(6):554-556.

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Chinese Journal of Reparative and Reconstructive Surgery

EXPRESSIONS OF OSTEOGENESIS RELATED FACTORS IN MG63 CELLS CO-CULTURED WITH DOMESTIC POROUS TANTALUM

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