PREPARATION AND BIOCOMPATIBILITY OF POLYURETHANE MICROSPHERES FOR BIOMEDICAL APPLICATIONS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

DALincui ¹ , WANGMin ¹ ^Δ , GONGMei ¹ ,  ZHANGLi ² ^Δ ,  XIEHuiqi ¹

1. Laboratory of Stem Cell and Tissue Engineering, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China;
2. Analytical & Testing Center, Research Center for Nano-biomaterials, Sichuan Universit;

Corresponding author：

ZHANGLi, Email: zhangli9111@126.com; XIEHuiqi, Email: xiehuiqi@163.com

Keywords：

Polyurethane microspheres; Human umbilical vein endothelial cells; Soft tissue filler; Blood compatibility

DOI：

10.7507/1002-1892.20150134

Video：

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Objective To prepare polyurethane (PU) microspheres and evaluate its physicochemical properties and biocompatibility for biomedical applications in vitro. Methods The PU microspheres were prepared by self-emulsification procedure at the emulsification rates of 1 000, 2 000, 3 000, and 4 000 r/min. The molecular structure was tested by Fourier transform infrared spectrometer and the surface and interior morphology of PU microspheres were observed by scanning electron microscopy (SEM). PU microspheres prepared at best emulsification rate were selected for the subsequent experiment. The human umbilical vein endothelial cells (HUVECs) were cultured and seeded on the materials, then cell morphology and adhesion status were observed by calcein-acetoxymethylester/pyridine iodide (Calcein-AM/PI) staining. The cells were cultured in the H-DMEM containing 10%FBS with additional 1% phenol (group A), in the extracts of PU prepared according to GB/T 16886.12 standard (group B), and in H-DMEM containing 10%FBS (group C), respectively. Cell counting kit 8 (CCK-8) assay was used to detect the cell viability. The blood compatibility experiments were used to evaluate the blood compatibility, the PU extracts as experimental group, stroke-physiological saline solution as negative control group, and distilled water as positive control group. The hemolytic rate was calculated. Results The SEM results of PU microspheres at the emulsification rate of 2 000 r/min showed better morphology and size. The microstructure of the PU was rough on the surface and porous inside. The Calcein-AM/PI staining showed that the HUVECs attached to the PU tightly and nearly all cells were stained by green. CCK-8 assays demonstrated that group B and group C presented a significantly higher cell proliferative activity than group A (P<0.05), indicating low cytotoxicity of the PU. The absorbance value was 0.864±0.002 in positive control group and was 0.015±0.001 in negative control group. The hemolysis rate of the PU extracts was 0.39%±0.07% (<5%), indicating no hemolysis. Conclusion The PU microspheres are successfully prepared by self-emulsification. The scaffold can obviously promote cell attachments and proliferation and shows low cytotoxicity and favorable blood compatibility, so it might be an ideal filler for soft tissue.

Citation： DALincui, WANGMin, GONGMei, ZHANGLi, XIEHuiqi. PREPARATION AND BIOCOMPATIBILITY OF POLYURETHANE MICROSPHERES FOR BIOMEDICAL APPLICATIONS. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(5): 626-631. doi: 10.7507/1002-1892.20150134 Copy

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24.	Lévesque SG, Lim RM, Shoichet MS. Macroporous interconnected dextran scaffolds of controlled porosity for tissue-engineering applications. Biomaterials, 2005, 26(35):7436-7446.

1. Cheung HK, Han TT, Marecak DM, et al. Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells. Biomaterials, 2014, 35(6):1914-1923.
2. Nash GM, Bleier J, Milsom JW, et al. Minimally invasive surgery is safe and effective for urgent and emergent colectomy. Colorectal Dis, 2010, 12(5):480-484.
3. 王学军, 徐冰, 刘文英, 等. Nuss微创漏斗胸矫形术后并发症及其处理. 中国修复重建外科杂志, 2009, 23(11):1343-1346.
4. Koshy ST, Ferrante TC, Lewin SA, et al. Injectable, porous, and cell-responsive gelatin cryogels. Biomaterials, 2014, 35(8):2477-2487.
5. Yang YP, Chien Y, Chiou GY, et al. Inhibition of cancer stem cell-like properties and reduced chemoradioresistance of glioblastoma using microRNA145 with cationic polyurethane-short branch PEI. Biomaterials, 2012, 33(5):1462-1476.
6. Nelson CE, Gupta MK, Adolph EJ, et al. Sustained local delivery of siRNA from an injectable scaffold. Biomaterials, 2012, 33(4):1154-1161.
7. Zhou L, Liang D, He X, et al. The degradation and biocompatibility of pH-sensitive biodegradable polyurethanes for intracellular multifunctional antitumor drug delivery. Biomaterials, 2012, 33(9):2734-2745.
8. Hu J, Chen B, Guo F, et al. Injectable silk fibroin/polyurethane composite hydrogel for nucleus pulposus replacement. J Mater Sci Mater Med, 2012, 23(3):711-722.
9. 厉孟, 刘旭东, 刘兴炎. 京尼平与戊二醛交联明胶微球的性能比较. 中国修复重建外科杂志, 2009, 23(1):87-91.
10. Zhou L, Liang D, He X, et al. The degradation and biocompatibility of pH-sensitive biodegradable polyurethanes for intracellular multifunctional antitumor drug delivery. Biomaterials, 2012, 33(9):2734-2745.
11. 程晓非, 邹德威, 吴继功, 等. 新型可注射型人工髓核材料的植入实验研究. 中国修复重建外科杂志, 2009, 23(6):670-676.
12. 田华科, 王建, 陈超, 等. 体外构建可注射式组织工程髓核的初步研究. 中国修复重建外科杂志, 2009, 23(2):173-177.
13. 陈伟, 刘仲前, 岳元辉, 等. bFGF壳聚糖微球的制备及检测. 中国修复重建外科杂志, 2012, 26(8):989-992.
14. Adolph EJ, Hafeman AE, Davidson JM, et al. Injectable polyurethane composite scaffolds delay wound contraction and support cellular infiltration and remodeling in rat excisional wounds. J Biomed Mater Res A, 2012, 100(2):450-461.
15. Xu D, Wu K, Zhang Q, et al. Synthesis and biocompatibility of anionic polyurethane nanoparticles coated with adsorbed chitosan. Polymer, 2010, 51(9):1926-1933.
16. Tan M, Feng Y, Wang H, et al. Immobilized bioactive agents onto polyurethane surface with heparin and phosphorylcholine group. Macromolecular Research, 2013, 21(5):541-549.
17. Xu D, Meng Z, Han M, et al. Novel blood-compatible waterborne polyurethane using chitosan as an extender. Journal of Applied Polymer Science, 2008, 109(1):240-246.
18. Das B, Chattopadhyay P, Mandal M, et al. Bio-based biodegradable and biocompatible hyperbranched polyurethane:a scaffold for tissue engineering. Macromol Biosci, 2013, 13(1):126-139.
19. Niu Y, Chen KC, He T, et al. Scaffolds from block polyurethanes based on poly (ε-caprolactone) (PCL) and poly(ethylene glycol) (PEG) for peripheral nerve regeneration. Biomaterials, 2014, 35(14):4266-4277.
20. 奚廷斐, 辛任东, 薛淼, 等. 医疗器械生物学评价. 北京:中国标准出版社, 2012:92-94, 234-235.
21. Ma S, Song G, Feng N. Preparation and characterization of self-emulsified waterborne nitrocellulose. Carbohydr Polym, 2012, 89(1):36-40.
22. 郭超. 季铵盐阳离子自乳化聚氨酯的合成及应用. 哈尔滨:东北林业大学, 2011.
23. 杨小明, 尹庆水, 张余, 等. 表面含硅微弧氧化涂层镁合金ZK60体外与成骨细胞生物相容性的研究. 中国修复重建外科杂志, 2013, 27(5):612-618.
24. Lévesque SG, Lim RM, Shoichet MS. Macroporous interconnected dextran scaffolds of controlled porosity for tissue-engineering applications. Biomaterials, 2005, 26(35):7436-7446.

Chinese Journal of Reparative and Reconstructive Surgery

PREPARATION AND BIOCOMPATIBILITY OF POLYURETHANE MICROSPHERES FOR BIOMEDICAL APPLICATIONS

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