EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

 WANGGuozhong , LIChengjun , FANXichao , LIBo , XIAOWei , JINLi

Department of Pathophysiology, Qiqihar Medical College, Qiqihar Heilongjiang, 161006, P. R. China;

Corresponding author：

WANGGuozhong, Email: wgzbs@163.com

Keywords：

Bone marrow mesenchymal stem cells; Gastric ulcer; Vascular endothelial growth factor; Ulcer healing; Rat

DOI：

10.7507/1002-1892.20150191

Video：

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Objective To explore the effect and mechanisms of bone marrow mesenchymal stem cells (BMSCs) on healing quality of acetic acid-induced gastric ulcer. Methods Forty-eight clean grade male Wistar rats were used to establish the model of gastric ulcer with acetic acid and were randomly divided into 3 groups after 3 days of modeling, 16 rats each group. After the abdominal cavity was open and stomach was pulled out, no treatment was given in group A, 150 μL phosphate buffered saline (PBS) and 150 μL BMSCs at passage 4+PBS (1×108 cells/100 μL) were injected into the gastric wall surrounding the ulcer at 5 different points in groups B and C respectively. After 10 days, the ulcer area was measured, the mucosal thickness and the number of dilated glands were tested in the regenerative mucosa by histological method. And the expression of vascular endothelial growth factor (VEGF) was detected at ulcerative margin by immunohistochemical method. Results The ulcer area in group C was significantly smaller than that of groups A and B (P<0.01), but no significant difference was found between groups A and B (P>0.05). HE staining showed that group C had thicker regenerative gastric mucosa, less dilated glands, and more regular mucosal structure than groups A and B, showing significant differences in regenerative gastric mucosa thickness and dilated glands number (P<0.01), but no significant difference between groups A and B (P>0.05). Immunohistochemical staining showed that the positive expression of VEGF in the ulcer margin mucosa of group C was significantly higher than that of groups A and B. The integral absorbance (IA) value of VEGF expression in group C was significantly higher than that in groups A and B (P<0.01), but no significant difference between groups A and B (P>0.05). Conclusion BMSCs can accelerate ulcer healing by the secretion of VEGF, and improve the quality of ulcer healing.

Citation： WANGGuozhong, LIChengjun, FANXichao, LIBo, XIAOWei, JINLi. EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(7): 889-892. doi: 10.7507/1002-1892.20150191 Copy

1.	Hoch AI,Leach JK.Concise review:optimizing expansion of bone marrow mesenchymal stem/stromal cells for clinical applications.Stem Cells Transl Med,2014,3(5):643-652.
2.	Hayashi Y,Tsuji S,Tsujii M,et al.Topical transplantation of mesenchymal stem cells accelerates gastric ulcer healing in rats.Am J Physiol Gastrointest Liver Physiol,2008,294(3):778-786.
3.	Nishida T,Tsuji S,Tsujii M,et al.Cultured bone marrow cell local implantation accelerates healing of ulcers in mice.J Gastroenterol,2008,43(2):124-135.
4.	刘韩,崔伟龙,牟林,等.阿司匹林促进胃酸分泌的途径与溃疡复发.中国医药导报,2010,7(34):16-18.
5.	王国忠,肖薇,李波.阿司匹林对大鼠醋酸胃溃疡愈合的影响.中国医院药学杂志,2013,33(23):1925-1928.
6.	王国忠,夏立丁,崔伟龙,等.幽门螺杆菌与阿司匹林对溃疡愈合的影响.中国全科医学,2011,14(2C):586-588,591.
7.	Liu P,Feng Y,Wang Y,et al.Therapeutic action of bone marrow-derived stem cells against acute kidney injury.Life Sci,2014,115(1-2):1-7.
8.	王贵均,林麟孙,香钰鸿.骨髓间充质干细胞治疗脑出血的临床观察.现代预防医学,2011,38(23):5018-5019.
9.	Hang HL,Xia Q.Role of BMSCs in liver regeneration and metastasis after hepatectomy.World J Gastroenterol,2014,20(1):126-132.
10.	王锋,吴小涛,王运涛,等.干细胞移植修复椎间盘退变的研究现状.中国修复重建外科杂志,2013,27(5):575-579.
11.	杨国强,范学慧,萧洪文.骨髓间充质干细胞移植治疗缺血性脑损伤的最新研究进展.四川解剖学杂志,2013,21(3):32-35.
12.	梁涛,刘浩.间充质干细胞移植治疗椎间盘退变疾病的研究进展.中国修复重建外科杂志,2007,21(5):502-506.
13.	Kagami H,Agata H,Tojo A.Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for bone tissue engineering:basic science to clinical translation.Int J Biochem Cell Biol,2011,43(3):286-289.
14.	焦雪峰,黄永灿,黄益洲,等.大鼠BMSCs自发钙化过程中成骨相关基因表达谱的分析.中国修复重建外科杂志,2014,28(2):133-141.
15.	韩冰,付小兵.间充质干细胞的研究进展与临床应用前景.中国修复重建外科杂志,2006,20(12):1257-1261.
16.	李成军,王丽萍,夏立丁,等.大鼠乙酸胃溃疡愈合过程中EGF和VEGF对胃黏膜组织形态的影响.医学研究杂志,2010,39(8):56-59.
17.	Liu Y,Dulchavsky DS,Gao X,et al.Wound repair by bone marrow stromal cells through growth factor production.J Surg Res,2006,136(2):336-341.
18.	Chang Q,Yan L,Wang CZ,et al.In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats.Chin Med J (Engl),2012,125(6):1169-1174.
19.	Askarov MB,Onischenko NA.Multipotent mesenchymal stromal cells of autologous bone marrow stimulate neoangiogenesis,restore microcirculation,and promote healing of indolent ulcers of the stomach.Bull Exp Biol Med,2008,146(4):512-516.
20.	晏开力,汪健,李庆,等.成人骨髓间充质干细胞分化为血管内皮细胞的研究.中国修复重建外科杂志,2007,21(1):76-80.
21.	张明珠,俞光荣.干细胞移植治疗糖尿病足的基础研究与临床应用进展.中国修复重建外科杂志,2009,23(3):358-361.

1. Hoch AI,Leach JK.Concise review:optimizing expansion of bone marrow mesenchymal stem/stromal cells for clinical applications.Stem Cells Transl Med,2014,3(5):643-652.
2. Hayashi Y,Tsuji S,Tsujii M,et al.Topical transplantation of mesenchymal stem cells accelerates gastric ulcer healing in rats.Am J Physiol Gastrointest Liver Physiol,2008,294(3):778-786.
3. Nishida T,Tsuji S,Tsujii M,et al.Cultured bone marrow cell local implantation accelerates healing of ulcers in mice.J Gastroenterol,2008,43(2):124-135.
4. 刘韩,崔伟龙,牟林,等.阿司匹林促进胃酸分泌的途径与溃疡复发.中国医药导报,2010,7(34):16-18.
5. 王国忠,肖薇,李波.阿司匹林对大鼠醋酸胃溃疡愈合的影响.中国医院药学杂志,2013,33(23):1925-1928.
6. 王国忠,夏立丁,崔伟龙,等.幽门螺杆菌与阿司匹林对溃疡愈合的影响.中国全科医学,2011,14(2C):586-588,591.
7. Liu P,Feng Y,Wang Y,et al.Therapeutic action of bone marrow-derived stem cells against acute kidney injury.Life Sci,2014,115(1-2):1-7.
8. 王贵均,林麟孙,香钰鸿.骨髓间充质干细胞治疗脑出血的临床观察.现代预防医学,2011,38(23):5018-5019.
9. Hang HL,Xia Q.Role of BMSCs in liver regeneration and metastasis after hepatectomy.World J Gastroenterol,2014,20(1):126-132.
10. 王锋,吴小涛,王运涛,等.干细胞移植修复椎间盘退变的研究现状.中国修复重建外科杂志,2013,27(5):575-579.
11. 杨国强,范学慧,萧洪文.骨髓间充质干细胞移植治疗缺血性脑损伤的最新研究进展.四川解剖学杂志,2013,21(3):32-35.
12. 梁涛,刘浩.间充质干细胞移植治疗椎间盘退变疾病的研究进展.中国修复重建外科杂志,2007,21(5):502-506.
13. Kagami H,Agata H,Tojo A.Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for bone tissue engineering:basic science to clinical translation.Int J Biochem Cell Biol,2011,43(3):286-289.
14. 焦雪峰,黄永灿,黄益洲,等.大鼠BMSCs自发钙化过程中成骨相关基因表达谱的分析.中国修复重建外科杂志,2014,28(2):133-141.
15. 韩冰,付小兵.间充质干细胞的研究进展与临床应用前景.中国修复重建外科杂志,2006,20(12):1257-1261.
16. 李成军,王丽萍,夏立丁,等.大鼠乙酸胃溃疡愈合过程中EGF和VEGF对胃黏膜组织形态的影响.医学研究杂志,2010,39(8):56-59.
17. Liu Y,Dulchavsky DS,Gao X,et al.Wound repair by bone marrow stromal cells through growth factor production.J Surg Res,2006,136(2):336-341.
18. Chang Q,Yan L,Wang CZ,et al.In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats.Chin Med J (Engl),2012,125(6):1169-1174.
19. Askarov MB,Onischenko NA.Multipotent mesenchymal stromal cells of autologous bone marrow stimulate neoangiogenesis,restore microcirculation,and promote healing of indolent ulcers of the stomach.Bull Exp Biol Med,2008,146(4):512-516.
20. 晏开力,汪健,李庆,等.成人骨髓间充质干细胞分化为血管内皮细胞的研究.中国修复重建外科杂志,2007,21(1):76-80.
21. 张明珠,俞光荣.干细胞移植治疗糖尿病足的基础研究与临床应用进展.中国修复重建外科杂志,2009,23(3):358-361.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING

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