EXPERIMENTAL STUDY ON EFFECT OF NICOTINE INTAKE ON IMPACT OF BONE MICROSTRUCTURE AND OXIDATIVE STRESS IN RATS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

YANGYang ¹ , WANGYawei ² ^△ ,  MAXinlong ¹ , MAJianxiong ³ , XINGDan ⁴ , ZHUShaowen ¹ , CHENYang ¹

1. Department of Orthopaedics, Tianjin Hospital, Tianjin, 300211, P. R. China;
2. Department of Electromyography, Tianjin Hospital, Tianjin, 300211, P. R. China;
3. Biomechanics Laboratory, Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine;
4. Department of Joint Surgery, Peking University People's Hospital;

Corresponding author：

MAXinlong, Email: maxinlong8686@sina.com

Keywords：

Nicotine; Oxidative stress; Osteoporosis; Biomechanics; Rat

DOI：

10.7507/1002-1892.20160309

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To evaluate the influence of nicotine intake on bone microstructure, bone biomechanics, and oxidative stress state in rats. Methods Thirty-six 6-week-old male Sprague Dawley rats (weight, 160-180 g) were randomly divided into control group, low dose group, and high dose group, 12 rats each group. The rats in high dose group and low dose group were given respectively 6.0 mg/kg and 0.4 mg/kg nicotine gavage intervention for 12 months; no intervention was made in the control group. The survival of rats was observed during experiment, and the weight of rats was measured every month. At 12 months after modeling, the L1 vertebral body was harvested to measure the bone mineral density (BMD), bone volume fraction (BVF), trabecular thickness (TT), trabecular number (TN), and trabecular spacing (TS) by Micro-CT three-dimensional reconstruction; the left femur was harvested for biomechanical tests of maximal load, stiffness, and the maximal fracture energy; and arterial blood was extracted to measure the malonyldialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and cotinine. Results During the experiment, two rats and one rat were added in the high dose group and the low dose group because of death, and no death in the control group. The body weight of the rats in the high and low dose groups gradually decreased with time when compared with one in the control group, and significant difference was found between two dose groups and the control group at 8-12 months (P < 0.05); the body weight of the high dose group was significantly lower than that of the low dose group at 11 and 12 months (P < 0.05). At 12 months after modeling, BMD, BVF, TT, and TN were significantly lower in the high dose group than the control group and the low dose group, but TS was significantly increased (P < 0.05). Difference in BVF, TN, and TS was significant between the low dose group and the control group (P < 0.05). The maximal load, stiffness, and maximal fracture energy of femoral shaft were significantly lower in the high dose group than the control group and the low dose group, and in the low dose group than the control group (P < 0.05). Compared with the control group, the levels of cotinine and MDA were significantly increased, and the levels of CAT and SOD were significantly decreased in the high and low dose groups (P < 0.05), and there were significant differences between the high and low dose groups (P < 0.05). Conclusion Nicotine intake can cause micro-structural changes of the bone, decreased bone mechanical properties, and imbalance of oxidation-antioxidant levels in rats. High-dose nicotine intake may be one of the causes of osteoporosis.

Citation： YANGYang, WANGYawei, MAXinlong, MAJianxiong, XINGDan, ZHUShaowen, CHENYang. EXPERIMENTAL STUDY ON EFFECT OF NICOTINE INTAKE ON IMPACT OF BONE MICROSTRUCTURE AND OXIDATIVE STRESS IN RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(12): 1493-1497. doi: 10.7507/1002-1892.20160309 Copy

1.	O'Connor KM. Evaluation and Treatment of Osteoporosis. Med Clin North Am, 2016, 100(4):807-826.
2.	Øyen J, Svingen GF, Gjesdal CG, et al. Plasma dimethylglycine, nicotine exposure and risk of low bone mineral density and hip fracture:the Hordaland Health Study. Osteoporos Int, 2015, 26(5):1573-1583.
3.	Abate M, Vanni D, Pantalone A, et al. Cigarette smoking and musculoskeletal disorders. Muscles Ligaments Tendons J, 2013, 3(2):63-69.
4.	Calderón-Garcidueñas L. Smoking and cerebral oxidative stress and air pollution:a dreadful equation with particulate matter involved and one more powerful reason not to smoke anything! J Alzheimers Dis, 2016, 54(1):109-112.
5.	Turan V, Mizrak S, Yurekli B, et al. The effect of long-term nicotine exposure on bone mineral density and oxidative stress in female Swiss Albino rats. Arch Gynecol Obstet, 2013, 287(2):281-287.
6.	Dhouib H, Jallouli M, Draief M, et al. Oxidative damage and histopathological changes in lung of rat chronically exposed to nicotine alone or associated to ethanol. Pathol Biol (Paris), 2015, 63(6):258-267.
7.	Conceição EP, Peixoto-Silva N, Pinheiro CR, et al. Maternal nicotine exposure leads to higher liver oxidative stress and steatosis in adult rat offspring. Food Chem Toxicol, 2015, 78:52-59.
8.	Mizrak S, Turan V, Inan S, et al. Effect of nicotine on RANKL and OPG and bone mineral density. J Invest Surg, 2014, 27(6):327-331.
9.	Hapidin H, Othman F, Soelaiman IN, et al. Effects of nicotine administration and nicotine cessation on bone histomorphometry and bone biomarkers in Sprague-Dawley male rats. Calcif Tissue Int, 2011, 88(1):41-47.
10.	Iwaniec UT, Haynatzki GR, Fung YK, et al. Effects of nicotine on bone and calciotropic hormones in aged ovariectomized rats. J Musculoskelet Neuronal Interact, 2002, 2(5):469-478.
11.	Ozokutan BH, Ozkan KU, Sari I, et al. Effects of maternal nicotine exposure during lactation on breast-fed rat pups. Biol Neonate, 2005, 88(2):113-117.
12.	Husgafvel-Pursiainen K. Biomarkers in the assessment of exposure and the biological effects of environmental tobacco smoke. Scand J Work Environ Health, 2002, 28 Suppl 2:21-29.
13.	Fan J, Zhang WX, Rao YS, et al. Perinatal nicotine exposure increases obesity susceptibility in adult male rat offspring by altering early adipogenesis. Endocrinology, 2016, 157(11):4276-4286.
14.	Fan J, Ping J, Zhang WX, et al. Prenatal and lactation nicotine exposure affects morphology and function of brown adipose tissue in male rat offspring. Ultrastruct Pathol, 2016, 40(5):288-295.
15.	Gueye AB, Pryslawsky Y, Trigo JM, et al. The CB1 neutral antagonist AM4113 retains the therapeutic efficacy of the inverse agonist rimonabant for nicotine dependence and weight loss, with better psychiatric tolerability. Int J Neuropsychopharmacol, 2016.[Epub ahead of print].
16.	Rupprecht LE, Smith TT, Donny EC, et al. Self-administered nicotine suppresses body weight gain independent of food intake in male rats. Nicotine Tob Res, 2016, 18(9):1869-1876.
17.	Younes-Rapozo V, Moura EG, Manhães AC, et al. Neonatal nicotine exposure leads to hypothalamic gliosis in adult overweight rats. J Neuroendocrinol, 2015, 27(12):887-898.
18.	刘宏建, 杜靖远, 刘辉, 等.绝经后骨质疏松症山羊模型的建立及其意义.中华实验外科杂志, 2005, 22(10):1266-1267.
19.	Walker LM, Preston MR, Magnay JL, et al. Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture. Bone, 2001, 28(6):603-608.
20.	Rothem DE, Rothem L, Soudry M, et al. Nicotine modulates bone metabolism-associated gene expression in osteoblast cells. J Bone Miner Metab, 2009, 27(5):555-561.

1. O'Connor KM. Evaluation and Treatment of Osteoporosis. Med Clin North Am, 2016, 100(4):807-826.
2. Øyen J, Svingen GF, Gjesdal CG, et al. Plasma dimethylglycine, nicotine exposure and risk of low bone mineral density and hip fracture:the Hordaland Health Study. Osteoporos Int, 2015, 26(5):1573-1583.
3. Abate M, Vanni D, Pantalone A, et al. Cigarette smoking and musculoskeletal disorders. Muscles Ligaments Tendons J, 2013, 3(2):63-69.
4. Calderón-Garcidueñas L. Smoking and cerebral oxidative stress and air pollution:a dreadful equation with particulate matter involved and one more powerful reason not to smoke anything! J Alzheimers Dis, 2016, 54(1):109-112.
5. Turan V, Mizrak S, Yurekli B, et al. The effect of long-term nicotine exposure on bone mineral density and oxidative stress in female Swiss Albino rats. Arch Gynecol Obstet, 2013, 287(2):281-287.
6. Dhouib H, Jallouli M, Draief M, et al. Oxidative damage and histopathological changes in lung of rat chronically exposed to nicotine alone or associated to ethanol. Pathol Biol (Paris), 2015, 63(6):258-267.
7. Conceição EP, Peixoto-Silva N, Pinheiro CR, et al. Maternal nicotine exposure leads to higher liver oxidative stress and steatosis in adult rat offspring. Food Chem Toxicol, 2015, 78:52-59.
8. Mizrak S, Turan V, Inan S, et al. Effect of nicotine on RANKL and OPG and bone mineral density. J Invest Surg, 2014, 27(6):327-331.
9. Hapidin H, Othman F, Soelaiman IN, et al. Effects of nicotine administration and nicotine cessation on bone histomorphometry and bone biomarkers in Sprague-Dawley male rats. Calcif Tissue Int, 2011, 88(1):41-47.
10. Iwaniec UT, Haynatzki GR, Fung YK, et al. Effects of nicotine on bone and calciotropic hormones in aged ovariectomized rats. J Musculoskelet Neuronal Interact, 2002, 2(5):469-478.
11. Ozokutan BH, Ozkan KU, Sari I, et al. Effects of maternal nicotine exposure during lactation on breast-fed rat pups. Biol Neonate, 2005, 88(2):113-117.
12. Husgafvel-Pursiainen K. Biomarkers in the assessment of exposure and the biological effects of environmental tobacco smoke. Scand J Work Environ Health, 2002, 28 Suppl 2:21-29.
13. Fan J, Zhang WX, Rao YS, et al. Perinatal nicotine exposure increases obesity susceptibility in adult male rat offspring by altering early adipogenesis. Endocrinology, 2016, 157(11):4276-4286.
14. Fan J, Ping J, Zhang WX, et al. Prenatal and lactation nicotine exposure affects morphology and function of brown adipose tissue in male rat offspring. Ultrastruct Pathol, 2016, 40(5):288-295.
15. Gueye AB, Pryslawsky Y, Trigo JM, et al. The CB1 neutral antagonist AM4113 retains the therapeutic efficacy of the inverse agonist rimonabant for nicotine dependence and weight loss, with better psychiatric tolerability. Int J Neuropsychopharmacol, 2016.[Epub ahead of print].
16. Rupprecht LE, Smith TT, Donny EC, et al. Self-administered nicotine suppresses body weight gain independent of food intake in male rats. Nicotine Tob Res, 2016, 18(9):1869-1876.
17. Younes-Rapozo V, Moura EG, Manhães AC, et al. Neonatal nicotine exposure leads to hypothalamic gliosis in adult overweight rats. J Neuroendocrinol, 2015, 27(12):887-898.
18. 刘宏建, 杜靖远, 刘辉, 等.绝经后骨质疏松症山羊模型的建立及其意义.中华实验外科杂志, 2005, 22(10):1266-1267.
19. Walker LM, Preston MR, Magnay JL, et al. Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture. Bone, 2001, 28(6):603-608.
20. Rothem DE, Rothem L, Soudry M, et al. Nicotine modulates bone metabolism-associated gene expression in osteoblast cells. J Bone Miner Metab, 2009, 27(5):555-561.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON EFFECT OF NICOTINE INTAKE ON IMPACT OF BONE MICROSTRUCTURE AND OXIDATIVE STRESS IN RATS

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content