Mechanism of lipolytic and smooth effects of D980-nm laser treatment on skin tissue in rats_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

HUANGXinlong ,  ZHANGWei , MIAOYibin , XIAHuakuan , WANGChao , WANGYouwen

Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi Heilongjiang, 154002, P.R.China;

Corresponding author：

ZHANGWei, Email: kinwai01@163.com

Keywords：

Laser; photodamage; fibroblasts; collagen fiber; transforming growth factor β₁; basic fibroblast growth factor; rat

DOI：

10.7507/1002-1892.201610012

Video：

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Objective To determine the efficacy of D980-nm laser in dissolving fat and renewing skin, and to explore the clinical application of D980-nm laser in reconstruction of photodamaged skin. Methods Eighteen 12-14 month-old male Sprague-Dawley rats, weighing 400-450 g, were randomly divided into 3 groups (n=6). The rat skin at the left side was exposed to D980-nm laser irradiation at a density of 20 J/cm², a power of 8 W, a pulse width of 20 ms, and a pulse frequency of 40 Hz for 1 time (group A), 2 times of 5-minute interval (group B), and 3 times of 5-minute interval (group C) as a treatment course, for 4 treatment courses with an interval of 1 week; the other side of the skin was not treated as the control groups (groups A1, B1, and C1, respectively). After 8 weeks, the skin was harvested for HE staining and immunohistochemical staining to observe the structure changes of skin, to measure the dermal thickness, to count the number of fibroblasts, and detect the expressions of transforming growth factor β₁ (TGF-β₁) and basic fibroblast growth factor (bFGF). Results Compared with groups A1, B1, and C1, the skin structure was significantly improved in groups A, B, and C. After D980-nm laser irradiation, the number of fat cells decreased; local angiogenesis was observed; the total number of fibroblasts and fibers increased; the collagen fiber had large diameter, and arranged closely and regularly; the dermal thickness and the number of the fibroblasts increased; and the expressions of TGF-β₁ and bFGF were significantly enhanced, showing significant differences (P<0.05). With increased D980-nm laser irradiation times, the above indexes increased, showing significant differences between group C and groups A, B (P<0.05). Conclusion D980-nm laser treatment has lipolytic and tender effect on the skin, and the frequency of the treatment is an important factor in skin renewal.

Citation： HUANGXinlong, ZHANGWei, MIAOYibin, XIAHuakuan, WANGChao, WANGYouwen. Mechanism of lipolytic and smooth effects of D980-nm laser treatment on skin tissue in rats. Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(2): 235-239. doi: 10.7507/1002-1892.201610012 Copy

1.	韦文峰, 邱柏程. 激光辅助吸脂术在面部轮廓塑形及年轻化中的应用. 中国美容医学杂志, 2015, 24(9): 11-14.
2.	Valizadeh N, Jalaly NY, Zarghampour M, et al. Evaluation of safety and efficacy of 980-nm diode laser-assisted lipolysis versus traditional liposuction for submental rejuvenation: A randomized clinical trial. J Cosmet Laser Ther, 2016, 18(1): 41-45.
3.	Ezra N, Arshanapalli A, Bednarek R, et al. The microsecond 1064nm Nd: YAG laser as an adjunct to improving surgical scars following Mohs micrographic surgery. Journal of Cosmetic & Laser Therapy, 2016, 21: 1-20.
4.	Kohl E, Meierhöfer J, Koller M, et al. Fractional carbon dioxide laser resurfacing of rhytides and photoaged skin--a prospective clinical study on patient expectation and satisfaction. Lasers Surg Med, 2015, 47(2): 111-119.
5.	Valizadeh N, Jalaly NY, Zarghampour M, et al. Evaluation of safety and efficacy of 980-nm diode laser-assisted lipolysis versus traditional liposuction for submental rejuvenation: A randomized clinical trial. J Cosmet Laser Ther, 2016, 18(1): 41-45.
6.	王婧薷, 陈苑雯, 李朦, 等. 脂肪源性血管基质成分对皮肤光老化的影响. 中国病理生理杂志, 2016, 32(5): 892-899.
7.	Golberg A, Khan S, Belov V, et al. Skin rejuvenation with non-invasive pulsed electric fields. Sci Rep, 2015, 5: 10187.
8.	De Luca C, Mikhal’chik EV, Suprun MV, et al. Skin antiageing and systemic redox effects of supplementation with marine collagen peptides and plant-derived antioxidants: a single-blind case-control clinical study. Oxid Med Cell Longev, 2016.[Epub ahead of print].
9.	赵娟, 李希宁, 李相军, 等. 自体皮肤成纤维细胞与毛发角蛋白复合填充材料在医学美容中的应用.中国生物制品学杂志, 2012, 25(2): 220-223.
10.	Wang Z, Zhong H, Yang Z, et al. Exogenous bFGF or TGF-β₁ accelerates healing of reconstructed dura by CO₂ laser soldering in minipigs. Lasers in Medical Science, 2013, 29(3): 1165-1171.
11.	卢勇舟, 陈亮, 毕波, 等. 胶原蛋白碎片对光老化真皮细胞外基质的影响. 中国美容整形外科杂志, 2015, 26(4): 213-215.
12.	Smithmyer ME, Sawicki LA, Kloxin AM. Hydrogel scaffolds as in vitro models to study fibroblast activation in wound healing and disease. Biomater Sci, 2014, 2(5): 634-650.
13.	Zuo C, Liang P, Huang X. Role of PPARbeta in fibroblast response to heat injury. Indian J Biochem Biophys, 2012, 49(4): 219-227.
14.	Fournier N, Dahan S, Barneon G, et al. Nonablative remodeling: a 14-month clinical ultrasound imaging and profilometric evaluation of a 1540 nm Er: Glass laser. Dermatol Surg, 2002, 28(10): 926-931.
15.	Quan T, Wang F, Shao Y, et al. Enhancing structural support of the dermal microenvironment activates fibroblasts, endothelial cells, and keratinocytes in aged human skin in vivo. J Invest Dermatol, 2013, 133(3): 658-667.
16.	Kuk H, Hutchenreuther J, Murphy-Marshman H, et al. 5Z-7-Oxozeanol inhibits the effects of TGFβ₁ on human gingival fibroblasts. PLoS One, 2015, 10(4): e0123689.
17.	Powers CJ, McLeskey SW, Wellstein A. Fibroblasts growth factors, their receptors and signaling. Endocr Relat Cancer, 2000, 7(3): 165-197.
18.	Abraham JA, Klagsbrun M. Modulation of wound repair by members of the fibroblast growth factor family//Clark RAF, ed. The molecular and cellular biology of wound repair. 2nd ed. New York: Plenum Press, 1996: 195-248.
19.	Sogabe Y, Abe M, Yoloyama Y, et al. Basic fibroblast growth factor stimulates human keratinocyte motility by Rac activation. Wound Rep Reg, 2006, 14(4): 457-462.
20.	Yan X, Xu H, Liu Y, et al. Effect of VEGF and bFGF concentration gradient on proliferation and migration of vascular endothelial cells and fibroblast. J Biomater Tissue Eng, 2015, 5(12): 930-936.
21.	Gallego-Muñoz P, Ibares-Frías L, Gattote JA. Human corneal fibroblast migration and ECM synthesis during stromal repair: Role played by PDGF-BB, bFGF, and TGFβ₁. Journal of Tissue Engineering and Regenerative Medicine, 2016.[Epub ahead of print].
22.	Kim MS, Song HJ, Lee SH, et al. Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts. J Cosmet Dermatol, 2014, 13(1): 44-51.

1. 韦文峰, 邱柏程. 激光辅助吸脂术在面部轮廓塑形及年轻化中的应用. 中国美容医学杂志, 2015, 24(9): 11-14.
2. Valizadeh N, Jalaly NY, Zarghampour M, et al. Evaluation of safety and efficacy of 980-nm diode laser-assisted lipolysis versus traditional liposuction for submental rejuvenation: A randomized clinical trial. J Cosmet Laser Ther, 2016, 18(1): 41-45.
3. Ezra N, Arshanapalli A, Bednarek R, et al. The microsecond 1064nm Nd: YAG laser as an adjunct to improving surgical scars following Mohs micrographic surgery. Journal of Cosmetic & Laser Therapy, 2016, 21: 1-20.
4. Kohl E, Meierhöfer J, Koller M, et al. Fractional carbon dioxide laser resurfacing of rhytides and photoaged skin--a prospective clinical study on patient expectation and satisfaction. Lasers Surg Med, 2015, 47(2): 111-119.
5. Valizadeh N, Jalaly NY, Zarghampour M, et al. Evaluation of safety and efficacy of 980-nm diode laser-assisted lipolysis versus traditional liposuction for submental rejuvenation: A randomized clinical trial. J Cosmet Laser Ther, 2016, 18(1): 41-45.
6. 王婧薷, 陈苑雯, 李朦, 等. 脂肪源性血管基质成分对皮肤光老化的影响. 中国病理生理杂志, 2016, 32(5): 892-899.
7. Golberg A, Khan S, Belov V, et al. Skin rejuvenation with non-invasive pulsed electric fields. Sci Rep, 2015, 5: 10187.
8. De Luca C, Mikhal’chik EV, Suprun MV, et al. Skin antiageing and systemic redox effects of supplementation with marine collagen peptides and plant-derived antioxidants: a single-blind case-control clinical study. Oxid Med Cell Longev, 2016.[Epub ahead of print].
9. 赵娟, 李希宁, 李相军, 等. 自体皮肤成纤维细胞与毛发角蛋白复合填充材料在医学美容中的应用.中国生物制品学杂志, 2012, 25(2): 220-223.
10. Wang Z, Zhong H, Yang Z, et al. Exogenous bFGF or TGF-β₁ accelerates healing of reconstructed dura by CO₂ laser soldering in minipigs. Lasers in Medical Science, 2013, 29(3): 1165-1171.
11. 卢勇舟, 陈亮, 毕波, 等. 胶原蛋白碎片对光老化真皮细胞外基质的影响. 中国美容整形外科杂志, 2015, 26(4): 213-215.
12. Smithmyer ME, Sawicki LA, Kloxin AM. Hydrogel scaffolds as in vitro models to study fibroblast activation in wound healing and disease. Biomater Sci, 2014, 2(5): 634-650.
13. Zuo C, Liang P, Huang X. Role of PPARbeta in fibroblast response to heat injury. Indian J Biochem Biophys, 2012, 49(4): 219-227.
14. Fournier N, Dahan S, Barneon G, et al. Nonablative remodeling: a 14-month clinical ultrasound imaging and profilometric evaluation of a 1540 nm Er: Glass laser. Dermatol Surg, 2002, 28(10): 926-931.
15. Quan T, Wang F, Shao Y, et al. Enhancing structural support of the dermal microenvironment activates fibroblasts, endothelial cells, and keratinocytes in aged human skin in vivo. J Invest Dermatol, 2013, 133(3): 658-667.
16. Kuk H, Hutchenreuther J, Murphy-Marshman H, et al. 5Z-7-Oxozeanol inhibits the effects of TGFβ₁ on human gingival fibroblasts. PLoS One, 2015, 10(4): e0123689.
17. Powers CJ, McLeskey SW, Wellstein A. Fibroblasts growth factors, their receptors and signaling. Endocr Relat Cancer, 2000, 7(3): 165-197.
18. Abraham JA, Klagsbrun M. Modulation of wound repair by members of the fibroblast growth factor family//Clark RAF, ed. The molecular and cellular biology of wound repair. 2nd ed. New York: Plenum Press, 1996: 195-248.
19. Sogabe Y, Abe M, Yoloyama Y, et al. Basic fibroblast growth factor stimulates human keratinocyte motility by Rac activation. Wound Rep Reg, 2006, 14(4): 457-462.
20. Yan X, Xu H, Liu Y, et al. Effect of VEGF and bFGF concentration gradient on proliferation and migration of vascular endothelial cells and fibroblast. J Biomater Tissue Eng, 2015, 5(12): 930-936.
21. Gallego-Muñoz P, Ibares-Frías L, Gattote JA. Human corneal fibroblast migration and ECM synthesis during stromal repair: Role played by PDGF-BB, bFGF, and TGFβ₁. Journal of Tissue Engineering and Regenerative Medicine, 2016.[Epub ahead of print].
22. Kim MS, Song HJ, Lee SH, et al. Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts. J Cosmet Dermatol, 2014, 13(1): 44-51.

Chinese Journal of Reparative and Reconstructive Surgery

Mechanism of lipolytic and smooth effects of D980-nm laser treatment on skin tissue in rats

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