ZHAO Yahong 1,2 , NIU Changmei 3 , GONG Jiahuan 2 , WANG Hongbo 1 , YANG Yumin 1,2
  • 1. College of Textile and Clothing, Jiangnan University, Wuxi Jiangsu, 214122, P.R.China;
  • 2. Key Laboratory of Neuro-regeneration of Jiangsu Province, Nantong University, Nantong Jiangsu, 226001, P.R.China;
  • 3. Medical School, Nantong University, Nantong Jiangsu, 226001, P.R.China;
YANG Yumin, Email: yangym@ntu.edu.cn
Electrospinning; graphene; silk fibroin; nanofilm; electrical conductivity; Schwann cells
10.7507/1002-1892.201703046
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Objective  To explore the construction and biocompatibility in vitro evaluation of the electrospun-graphene (Gr)/silk fibroin (SF) nanofilms. Methods  The electrostatic spinning solution was prepared by dissolving SF and different mass ratio (0, 5%, 10%, 15%, and 20%) of Gr in formic acid solution. The hydrophilia and hydrophobic was analyzed by testing the static contact angle of electrostatic spinning solution of different mass ratio of Gr. Gr-SF nanofilms with different mass ratio (0, 5%, 10%, 15%, and 20%, as groups A, B, C, D, and E, respectively) were constructed by electrospinning technology. The structure of nanofilms were observed by optical microscope and scanning electron microscope; electrochemical performance of nanofilms were detected by cyclic voltammetry at electrochemical workstation; the porosity of nanofilms were measured by n-hexane substitution method, and the permeability were observed; L929 cells were used to evaluate the cytotoxicity of nanofilms in vitro at 1, 4, and 7 days after culture. The primary Sprague Dawley rats’ Schwann cells were co-cultured with different Gr-SF nanofilms of 5 groups for 3 days, the morphology and distribution of Schwann cells were identified by toluidine blue staining, the cell adhesion of Schwann cells were determined by cell counting kit 8 (CCK-8) method, the proliferation of Schwann cells were detected by EdU/Hoechst33342 staining. Results  The static contact angle measurement confirmed that the hydrophilia of Gr-SF electrospinning solution was decreased by increasing the mass ratio of Gr. Light microscope and scanning electron microscopy showed that Gr-SF nanofilms had nanofiber structure, Gr particles could be dispersed uniformly in the membrane, and the increasing of mass ratio of Gr could lead to the aggregation of particles. The porosity measurement showed that the Gr-SF nanofilms had high porosity (>65%). With the increasing of mass ratio of Gr, the porosity and conductivity of Gr-SF nanofilm increased gradually, the value in the group A was significantly lower than those in groups C, D, and E (P<0.05). In vitro L929 cells cytotoxicity test showed that all the Gr-SF nanofilms had good biocompatibility. Toluidine blue staining, CCK-8 assay, and EdU/Hoechst33342 staining showed that Gr-SF nanofilms with mass ratio of Gr less than 10% could support the survival and proliferation of co-cultured Schwann cells. Conclusion  The Gr-SF nanofilm with mass ratio of Gr less than 10% have proper hydrophilia, conductivity, porosity, and other physical and chemical properties, and have good biocompatibility in vitro. They can be used in tissue engineered nerve preparation.

Citation： ZHAO Yahong, NIU Changmei, GONG Jiahuan, WANG Hongbo, YANG Yumin. Construction and biocompatibility in vitro evaluation of electrospun-graphene/silk fibroin nanofilms . Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(9): 1119-1126. doi: 10.7507/1002-1892.201703046 Copy

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