CHEN Zaomei 1,2 , ZHAO Yue 1,2 , HE Ting 3 , CHEN Kejun 1,2 , JIANG Li 2 , ZHANG Junhui 3 , WAN Huayin 3 , LI Rubing 1,2
  • 1. Pharmaceutical college of Guangdong Pharmaceutical University, Guangzhou Guangdong, 510006, P.R.China;
  • 2. Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou Guangdong, 510010, P.R.China;
  • 3. Guangzhou Electrospinning Biological Technology Co.Ltd., Guangzhou Guangdong, 510604, P.R.China;
LI Rubing, Email: lrb90927@126.com
Intraperitoneal adhesion; electrospinning technology; anti-adhesion membrane; poly-L-lactic/glycolic acid; polyethylene glycol; biodegradability; rat
10.7507/1002-1892.201703083
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ObjectiveAdopting poly-L-lactic/glycolic acid (PLGA) and polyethylene glycol (PEG) as the material to fabricate PLGA/PEG electrospun polymer membrane by electrospinning technology. And to study its preventive effect on postoperative intraperitoneal adhesion of rat.MethodsPLGA and PEG were mixed at the ratio of 19∶1(M/M), then dissolved in organic solvent. The PLGA/PEG electrospun polymer membrane was prepared by electrospinning technology, and then the gross observation and scanning electron microscope observation were taken. Fifty-four Sprague Dawley rats (weighing, 180-200 g), were randomly divided into 3 groups. The rats in control group (n=6) were left intact. The rats in model group (n=24) and PLGA/PEG group (n=24) were treated with the method of mechanical injury of the cecal serosa in order to establish the intraperitoneal adhesion models; then the PLGA/PEG electrospun polymer membrane was used to cover the wound in PLGA/PEG group, but was not in the model group. The intraperitoneal adhesion in PLGA/PEG group and model group were observed at 3 days, 1 week, 2 weeks, and 8 weeks after operation, and the adhesion degree was assessed according to the self-generated standard. The degradation of PLGA/PEG electrospun polymer membrane was also observed in PLGA/PEG group. At each time point, the rats were harvested for histological observation. All the above indexes were compared with the control group.ResultsUsing the electrospinning technology, PLGA/PEG electrospun polymer membrane was prepared successfully. PLGA/PEG electrospun polymer membrane was white and opaque, with soft texture. Scanning electron microscopy observation showed that PLGA/PEG electrospun polymer membrane was mainly composed of disorderly staggered fibers, with microporous structure. All rats survived to the end of the experiment. Gross observation showed that PLGA/PEG electrospun polymer membrane gradually degraded after implantation in vivo, and the adhesion degree in PLGA/PEG group was significantly lower than that in model group (P<0.05), but it had not yet reached to the level of the control group (P<0.05). Histological observation showed that the proliferation of cecal fibrous connective tissue was slower in PLGA/PEG group than in model group, and adhesion severity significantly decreased, only with a small amount of inflammatory cell infiltration. Nevertheless, it was not up to the level of the control group.ConclusionPLGA/PEG electrospun polymer membrane can effectively prevent postoperative intraperitoneal adhesion of rat, and has good biodegradability.

Citation： CHEN Zaomei, ZHAO Yue, HE Ting, CHEN Kejun, JIANG Li, ZHANG Junhui, WAN Huayin, LI Rubing. Study on the prevention of postoperative intraperitoneal adhesion of rat with PLGA/PEG electrospun polymer membrane. Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(8): 999-1005. doi: 10.7507/1002-1892.201703083 Copy

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