Visualization research of three-dimensional microstructure of rabbit sciatic nerve bundles by micro-CT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

Yamuhanmode · Alike , Yilizati · Yilihamu , Alimujiang · Abulaiti , Maimaiaili · Yushan ,  Aihemaitijiang · Yusufu

Department of Microsurgical and Reconstruction, the First Affiliated Hospital of Xinjiang Medical University, Urumqi Xinjiang, 830054, P.R.China;

Corresponding author：

Aihemaitijiang · Yusufu, Email: ahmatjang@163.com

Keywords：

micro-CT; sciatic nerve; microstructure; three-dimensional visualization; rabbit

DOI：

10.7507/1002-1892.201705055

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To realize the visualization of three-dimensional microstructure of rabbit sciatic nerve bundles by micro-CT and three-dimensional visualization software Mimics17.0. Methods The sciatic nerve tissues from 6 New Zealand rabbits were divided into 2 groups (n=3), and the sciatic nerve tissues were stained by 1% (group A) and 5% (group B) Lugol solution respectively. After staining for 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, and 3.5 hours, the imaging changes of specimens were observed by light microscope and micro-CT. The clear micro-CT images were exported to the Mimics software to complete the visualization of three-dimensional microstructure of rabbit sciatic nerve according to three-dimensional reconstruction tool. Results The clear three-dimensional microstructure images could be observed in group A at 2.5 hours after staining and in group B at 1.5 hours after staining by light microscope and micro-CT. The sciatic nerve of New Zealand rabbits were divides into 3 bundles and each of them was relatively fixed. There was no obvious crossing or mergers between each bundle. The cross-sectional area of each bundle was (0.425±0.013), (0.038±0.007), and (0.242±0.026) mm² respectively. The digital model could clearly reflect the microstructure of the sciatic nerve at all cross sections. Conclusion The internal structure of New Zealand rabbits sciatic nerve can be clearly reflected by micro-CT scanning. It provides a reliable method for establishing a nerve microstructure database with large amount specimens.

Citation： Yamuhanmode · Alike, Yilizati · Yilihamu, Alimujiang · Abulaiti, Maimaiaili · Yushan, Aihemaitijiang · Yusufu. Visualization research of three-dimensional microstructure of rabbit sciatic nerve bundles by micro-CT. Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(12): 1490-1494. doi: 10.7507/1002-1892.201705055 Copy

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13.	Dinis TM, Elia R, Vidal G, et al. 3D multi-channel bi-functionalized silk electrospun conduits for peripheral nerve regeneration. J Mech Behav Biomed Mater, 2015, 41: 43-55.
14.	Reganne M, Röthlisberger M, Guzman R, et al. Dual Functional Collagen Nerve Conduits for Bridging Critical Nerve Gaps. Journal of Neurological Surgery Part A: Central European Neurosurgery, 2015, 76(01): 059. 【核！未查到】.
15.	Johnson BN, Lancaster KZ, Zhen G, et al. 3D printed anatomical nerve regeneration pathways. Adv Funct Mater, 2015, 25(39): 6205-6217.

1. Liu Z, Han N, Kou Y, et al. Electrophysiological and imaging outcomes analysis in patients with peripheral nerve injury treated with biodegradable conduit small-gap (2 mm) tubulization: A 5-year follow-up. International Journal of Clinical & Experimental Medicine, 2017, 10(5):7774-7784.【核！未查到】.
2. 朱家恺, 罗永湘, 陈统一. 现代周围神经外科学. 上海: 上海科学技术出版社, 2007: 404-405.
3. Lee JH, Lee JY, Yang SH, et al. Carbon nanotube-collagen three-dimensional culture of mesenchymal stem cells promotes expression of neural phenotypes and secretion of neurotrophic factors. Acta Biomater, 2014, 10(10): 4425-4436.
4. Hermenegildo JA, Roberts SL, Kim SY. Innervation pattern of the suprascapular nerve within supraspinatus: a three‐dimensional computer modeling study. Clin Anat, 2014, 27(4): 622-630.
5. Buytaert J, Goyens J, De Greef D, et al. Volume shrinkage of bone, brain and muscle tissue in sample preparation for micro-CT and light sheet fluorescence microscopy (LSFM). Microsc Microanal, 2014, 20(4): 1208-1217.
6. Carrera CA, Lan C, Escobar-Sanabria D, et al. The use of micro-CT with image segmentation to quantify leakage in dental restorations. Dent Mater, 2015, 31(4): 382-390.
7. Zhu S, Zhu Q, Liu X, et al. Three-dimensional reconstruction of the microstructure of human acellular nerve allograft. Sci Rep, 2016, 6: 30694.
8. Hopkins TM, Heilman AM, Liggett JA, et al. Combining micro-computed tomography with histology to analyze biomedical implants for peripheral nerve repair. J Neurosci Methods, 2015, 255: 122-130.
9. 陈增淦, 陈统一, 张键, 等. 臂丛神经显微结构的计算机三维重建. 中华骨科杂志, 2004, 24(8): 462-466.
10. Brill NA, Tyler DJ. Quantification of human upper extremity nerves and fascicular anatomy. Muscle Nerve, 2017, 56(3): 463-471.
11. 张升波, 刘海飞, 陈峰, 等. 周围神经损伤修复和治疗研究进展. 中华显微外科杂志, 2016, 39(2): 204-208.
12. Li A, Hokugo A, Yalom A, et al. A bioengineered peripheral nerve construct using aligned peptide amphiphile nanofibers. Biomaterials, 2014, 35(31): 8780-8790.
13. Dinis TM, Elia R, Vidal G, et al. 3D multi-channel bi-functionalized silk electrospun conduits for peripheral nerve regeneration. J Mech Behav Biomed Mater, 2015, 41: 43-55.
14. Reganne M, Röthlisberger M, Guzman R, et al. Dual Functional Collagen Nerve Conduits for Bridging Critical Nerve Gaps. Journal of Neurological Surgery Part A: Central European Neurosurgery, 2015, 76(01): 059. 【核！未查到】.
15. Johnson BN, Lancaster KZ, Zhen G, et al. 3D printed anatomical nerve regeneration pathways. Adv Funct Mater, 2015, 25(39): 6205-6217.

Chinese Journal of Reparative and Reconstructive Surgery

Visualization research of three-dimensional microstructure of rabbit sciatic nerve bundles by micro-CT

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