• 1. School of Stomatology, Southwest Medical University, Luzhou Sichuan, 646000, P.R.China;
  • 2. Department of Stomatology, Chengdu Hospital of Sichuan Petroleum Administration, Chengdu Sichuan, 610051, P.R.China;
  • 3. Department of Stomatology, Authority Hospital of Chengdu Military Region, Chengdu Sichuan, 610031, P.R.China;
  • 4. Department of Nuclear Medicine & Sichuan Key Laboratory of Nuclear Medicine and Molecular Imaging, Affiliated Hospital of Southwest Medical University, Luzhou Sichuan, 646000, P.R.China;
ZHONG Ke, Email: 37435387@qq.com
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Objective To explore the osteogenesis effect of advanced-platelet-rich fibrin (A-PRF) and β-tricalcium phosphate (β-TCP) composite. Methods Thirty-two healthy female New Zealand rabbits were randomly selected. A-PRF was prepared by collecting blood from middle auricular artery. Rabbits were randomly divided into 6 groups: groups A, B, C, D, and E (6 rabbits in each group) and group F (2 rabbits). Bone defects (6 mm in diameter, 8 mm in depth) were drilled into femur condyle of each rabbit’s both back legs. Then A-PRF and β-TCP composites manufactured by different proportion were planted into bone defects of group A (1∶1), group B (2∶1), group C (4∶1), group D (1∶2) and group E (1∶4) (V/V). The bone defect was not repaired in group F. The specimens were collected at 8 and at 12 weeks after operation. Then gross observation, X-ray examination, Micro-CT examination, and biomechanical test were performed. The bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular spacing (Tb.Sp), compressive strength, and modulus of elasticity were calculated. Results The gross observation and X-ray examination showed that the osteogenesis effect at 12 weeks was better than that at 8 weeks. At the same time point, the repair of bone defect and the formation of new bone in group B were better than those in other groups. Micro-CT examination showed that the trabeculae of new bone in group B were the most and the trabeculae arranged closely at 8 and 12 weeks. Besides there were significant differences in BV/TV, Tb.N, and Tb.Sp between group B and the other groups (P<0.05). There were significant differences in Tb.N and Tb.Th in group B, BV/TV and Tb.Sp in group C, Tb.Sp in group D between 8 weeks and 12 weeks (P<0.05). Biomechanical tests showed that the compression strength and elastic modulus of group B were the highest, and the compression strength and elastic modulus of group C were the lowest at 8 and at 12 weeks, showing significant differences (P<0.05). There were significant differences in compression strength and elastic modulus of each group between 8 weeks and 12 weeks (P<0.05). Conclusion The A-PRF and β-TCP composite can repair femoral condylar defects in rabbits, and the osteogenesis is better in proportion of 2∶1.

Citation: LI Xuemei, ZHANG Yushi, ZHONG Ke, LU Shuai, CHEN Yue. Study on the bone regeneration induced by advanced-platelet-rich fibrin and β- tricalcium phosphate composite. Chinese Journal of Reparative and Reconstructive Surgery, 2019, 33(2): 177-184. doi: 10.7507/1002-1892.201807002 Copy

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