Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β<sub>1</sub> and basic fibroblast growth factor induced membrane by Masquelet technology_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

DING Qiang , SUN Nan ,  WANG Xiaohui

Department of Osteonecrosis, Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, P.R.China;

Corresponding author：

WANG Xiaohui, Email: 963099784@qq.com

Keywords：

Bone defect; glycoside of short-horned epimedium Herb; Masquelet technique; osteogenic activity; transforming growth factor β₁; basic fibroblast growth factor

DOI：

10.7507/1002-1892.201905060

Video：

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Objective To observe the expressions of transforming growth factor β₁ (TGF-β₁) and basic fibroblast growth factor (bFGF) induced membrane by Masquelet technique in rats treated with glycoside of short-horned epimedium Herb, and to explore the effect of glycoside of short-horned epimedium Herb on Masquelet induced membrane.Methods Sixty 3-month-old male Wistar rats were randomly divided into 3 groups with 20 rats in each group; a tibial bone defect (6 mm in length) model was established. The blank group (group A) was not treated; the control group (group B) and the experimental group (group C) were filled with vancomycin antibiotic bone cement in the drawing stage, and the bone cement was completely solidified. Group C was given perfused flavonoids glycoside of short-horned epimedium Herb (10 μmol/L) by gavage once a day (0.3 mL) from 1 day after operation, and groups A and B were given the same amount of normal saline by gavage. After operation, the recovery and wound healing of experimental animals were observed; at 4 weeks after operation, X-ray film was taken to observe the recovery of bone defect of proximal tibia; at 6 weeks after operation, the bone defect was observed, and the morphological changes and vascularization degree of granulation tissue and induction membrane tissue were observed; the expressions of TGF-β₁ and bFGF were observed by immunohistochemistry staining and ELISA detection.Results The bone defect models of the 3 groups were established successfully, and there was no abnormality after operation. The incisions healed by first intention after operation. At 4 weeks after operation, X-ray films of proximal tibial defect showed that there was obvious space in group A, while bone cement was filled and Kirschner wire fixation was good in groups B and C. At 6 weeks after operation, the gross observation showed that the granulation tissue was filled in the defect area in group A; transparent membrane was formed in groups B and C, and microvessels were seen in some areas, and the microvessels in group C were significantly more than those in group B. Immunohistochemical staining showed that the expressions of TGF-β₁ and bFGF were negative in group A, but they were expressed in groups B and C, and the expressions of TGF-β₁ and bFGF in group B were significantly less than those in group C. ELISA detection showed that the expressions of TGF-β₁ and bFGF in group C were significantly higher than those in groups A and B (P<0.05), but there was no significant difference between groups A and B (P>0.05). Conclusion Glycoside of short-horned epimedium Herb can significantly increase the expressions of TGF-β₁ and bFGF, accelerate the process of osteogenesis, and contribute to bone shaping and reconstruction.

Citation： DING Qiang, SUN Nan, WANG Xiaohui. Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β₁ and basic fibroblast growth factor induced membrane by Masquelet technology. Chinese Journal of Reparative and Reconstructive Surgery, 2020, 34(11): 1405-1409. doi: 10.7507/1002-1892.201905060 Copy

1.	何伟, 李自力, 崔元璐, 等. 淫羊藿苷对大鼠成骨细胞核结合因子 α1、骨形成蛋白-2、骨形成蛋白-4 mRNA 表达的影响. 北京大学学报 (医学版), 2009, 41(6): 669-673.
2.	马秀武, 程迅生. 自体骨移植修复骨缺损的研究进展. 中国骨与关节损伤杂志, 2011, 26(6): 574-576.
3.	Wang XL, Wang NL, Zhang Y, et al. Effects of eleven flavonoids from the osteoprotective fraction of Drynaria fortunei (KUNZE) J. SM. on osteoblastic proliferation using an osteoblast-like cell line. Chem Pharm Bull (Tokyo), 2008, 56(1): 46-51.
4.	王日雄, 林建华, 修忠标, 等. 鹿茸多肽诱导自体骨髓间充质干细胞移植修复兔膝关节软骨缺损. 中国骨与关节损伤杂志, 2007, 22(11): 916-918.
5.	高怡加, 黄培镇, 李悦, 等. 骨碎补总黄酮对牵张成骨过程中骨形态发生蛋白-2 和转化生长因子-β₁ 表达的影响. 广州中医药大学学报, 2016, 33(5): 679-683.
6.	刘印, 贺利平, 田京. 不同时期 bFGF 或副甲状腺激素相关肽对 TGF-β₁ 诱导的兔 BMSCs 向软骨细胞分化的影响. 中国修复重建外科杂志, 2013, 27(2): 199-206.
7.	Gouron R, Petit L, Boudot C, et al. Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique. J Tissue Eng Regen Med, 2017, 11(2): 382-389.
8.	章元沛. 药理学实验. 北京: 人民卫生出版社, 1996: 28.
9.	刘剑刚, 谢雁鸣, 徐哲, 等. 骨碎补总黄酮的活血化瘀作用及对实验性微循环障碍和骨质疏松症的影响. 中国骨质疏松杂志, 2006, 12(1): 46-49.
10.	Apard T, Bigorre N, Cronier P, et al. Two-stage reconstruction of post-traumatic segmental tibia bone loss with nailing. Orthop Traumatol Surg Res, 2010, 96(5): 549-553.
11.	Wong RW, Rabie AB. Systemic effect of crude extract from rhizome of Drynaria fortunei on bone formation in mice. Phytother Res, 2006, 20(4): 313-315.
12.	Flamans B, Pauchot J, Petite H, et al. Use of the induced membrane technique for the treatment of bone defects in the hand or wrist, in emergency. Chir Main, 2010, 29(5): 307-314.
13.	Masquelet AC, Fitoussi F, Begue T, et al. Reconstruction of the long bones by the induced membrane and spongy autograft. Ann Chir Plast Esthet, 2000, 45(3): 346-353.
14.	高子范, 杨宗智, 马克昌, 等. 淫羊藿注射液对试管内鸡胚股骨生长的促进作用. 中西医结合杂志, 1985, 5(3): 172-174, 133.
15.	李树源, 周琦石, 李悦, 等. 不同组织部位诱导膜血管化及成骨因子表达的比较. 中国组织工程研究, 2020, 24(5): 667-672.
16.	蔡群斌, 李定, 李悦, 等. 骨碎补总黄酮对 Masquelet 技术诱导膜内血管形成的影响. 广州中医药大学学报, 2017, 34(6): 867-871.

1. 何伟, 李自力, 崔元璐, 等. 淫羊藿苷对大鼠成骨细胞核结合因子 α1、骨形成蛋白-2、骨形成蛋白-4 mRNA 表达的影响. 北京大学学报 (医学版), 2009, 41(6): 669-673.
2. 马秀武, 程迅生. 自体骨移植修复骨缺损的研究进展. 中国骨与关节损伤杂志, 2011, 26(6): 574-576.
3. Wang XL, Wang NL, Zhang Y, et al. Effects of eleven flavonoids from the osteoprotective fraction of Drynaria fortunei (KUNZE) J. SM. on osteoblastic proliferation using an osteoblast-like cell line. Chem Pharm Bull (Tokyo), 2008, 56(1): 46-51.
4. 王日雄, 林建华, 修忠标, 等. 鹿茸多肽诱导自体骨髓间充质干细胞移植修复兔膝关节软骨缺损. 中国骨与关节损伤杂志, 2007, 22(11): 916-918.
5. 高怡加, 黄培镇, 李悦, 等. 骨碎补总黄酮对牵张成骨过程中骨形态发生蛋白-2 和转化生长因子-β₁ 表达的影响. 广州中医药大学学报, 2016, 33(5): 679-683.
6. 刘印, 贺利平, 田京. 不同时期 bFGF 或副甲状腺激素相关肽对 TGF-β₁ 诱导的兔 BMSCs 向软骨细胞分化的影响. 中国修复重建外科杂志, 2013, 27(2): 199-206.
7. Gouron R, Petit L, Boudot C, et al. Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique. J Tissue Eng Regen Med, 2017, 11(2): 382-389.
8. 章元沛. 药理学实验. 北京: 人民卫生出版社, 1996: 28.
9. 刘剑刚, 谢雁鸣, 徐哲, 等. 骨碎补总黄酮的活血化瘀作用及对实验性微循环障碍和骨质疏松症的影响. 中国骨质疏松杂志, 2006, 12(1): 46-49.
10. Apard T, Bigorre N, Cronier P, et al. Two-stage reconstruction of post-traumatic segmental tibia bone loss with nailing. Orthop Traumatol Surg Res, 2010, 96(5): 549-553.
11. Wong RW, Rabie AB. Systemic effect of crude extract from rhizome of Drynaria fortunei on bone formation in mice. Phytother Res, 2006, 20(4): 313-315.
12. Flamans B, Pauchot J, Petite H, et al. Use of the induced membrane technique for the treatment of bone defects in the hand or wrist, in emergency. Chir Main, 2010, 29(5): 307-314.
13. Masquelet AC, Fitoussi F, Begue T, et al. Reconstruction of the long bones by the induced membrane and spongy autograft. Ann Chir Plast Esthet, 2000, 45(3): 346-353.
14. 高子范, 杨宗智, 马克昌, 等. 淫羊藿注射液对试管内鸡胚股骨生长的促进作用. 中西医结合杂志, 1985, 5(3): 172-174, 133.
15. 李树源, 周琦石, 李悦, 等. 不同组织部位诱导膜血管化及成骨因子表达的比较. 中国组织工程研究, 2020, 24(5): 667-672.
16. 蔡群斌, 李定, 李悦, 等. 骨碎补总黄酮对 Masquelet 技术诱导膜内血管形成的影响. 广州中医药大学学报, 2017, 34(6): 867-871.

Chinese Journal of Reparative and Reconstructive Surgery

Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β₁ and basic fibroblast growth factor induced membrane by Masquelet technology

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Chinese Journal of Reparative and Reconstructive Surgery

Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β1 and basic fibroblast growth factor induced membrane by Masquelet technology

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Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β₁ and basic fibroblast growth factor induced membrane by Masquelet technology