LIANG Zhijie 1 , HUANG Donglin 2 △ , ZHANG Muzi 2 , YI Xiaolin 2 , WU Fangxiao 2 , ZHU Dandan 1 , NING Yan 2 , GAN Huimin 3 , LI Hongmian 2
  • 1. Department of Wound Repair, the Fifth Affiliated Hospital of Guangxi Medical University, Nanning Guangxi, 530022, P.R.China;
  • 2. Department of Plastic Surgery, the Fifth Affiliated Hospital of Guangxi Medical University, Nanning Guangxi, 530022, P.R.China;
  • 3. Department of Radiotherapy, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning Guangxi, 530022, P.R.China;
LI Hongmian, Email: lihongmian@gxmu.edu.cn
Stromal cell-derived factor 1α; adipose derived stem cells; cell migration; stem cell chemotaxis; cell proliferation
10.7507/1002-1892.202004134
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Objective To explored the effect of stromal cell-derived factor 1α (SDF-1α) on promoting the migration ability of rat adipose derived stem cells (rADSCs) by constructed the rADSCs overexpression SDF-1α via adenovirus transfection.Methods rADSCs were isolated from adipose tissue of 6-week-old SPF Sprague Dawley rats. Morphological observation, multi-directional differentiations (osteogenic, adipogenic, and chondrogenic inductions), and flow cytometry identification were performed. Transwell cell migration experiment was used to observe and screen the optimal concentration of exogenous SDF-1α to optimize the migration ability of rADSCs; the optimal multiplicity of infection (MOI) of rADSCs was screened by observing the cell status and fluorescence expression after transfection. Then the third generation of rADSCs were divided into 4 groups: group A was pure rADSCs; group B was rADSCs co-cultured with SDF-1α at the best concentration; group C was rADSCs infected with recombinant adenovirus-mediated green fluorescent protein (Adv-GFP) with the best MOI; group D was rADSCs infected with Adv-GFP-SDF-1α overexpression adenovirus with the best MOI. Cell counting kit 8 (CCK-8) and Transwell cell migration experiment were preformed to detect and compare the effect of exogenous SDF-1α and SDF-1α overexpression on the proliferation and migration ability of rADSCs.Results The cell morphology, multi-directional differentiations, and flow cytometry identification showed that the cultured cells were rADSCs. After screening, the optimal stimulating concentration of exogenous SDF-1α was 12.5 nmol/L; the optimal MOI of Adv-GFP adenovirus was 200; the optimal MOI of Adv-GFP-SDF-1α overexpression adenovirus was 400. CCK-8 method and Transwell cell migration experiment showed that compared with groups A and C, groups B and D could significantly improve the proliferation and migration of rADSCs (P<0.05); the effect of group D on enhancing the migration of rADSCs was weaker than that of group B, but the effect of promoting the proliferation of rADSCs was stronger than that of group D (P<0.05).Conclusion SDF-1α overexpression modification on rADSCs can significantly promote the proliferation and migration ability, which may be a potential method to optimize the application of ADSCs in tissue regeneration and wound repair.

Citation： LIANG Zhijie, HUANG Donglin, ZHANG Muzi, YI Xiaolin, WU Fangxiao, ZHU Dandan, NING Yan, GAN Huimin, LI Hongmian. In vitro study on promoting migration ability of rat adipose derived stem cells modified by stromal cell-derived factor 1α. Chinese Journal of Reparative and Reconstructive Surgery, 2020, 34(10): 1305-1312. doi: 10.7507/1002-1892.202004134 Copy

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