Critical Steps and Complication Prevention of Heterotopic Abdominal Heart Transplantation in Rats_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

YULei ¹ ,  GAOLi ²

1. Department of General Surgery, Second Hospital of Harbin Medical University, Harbin 150086, P. R. China;
2. Department of Oral Surgery, Second Hospital of Harbin Medical University, Harbin 150086, P. R. China;

Corresponding author：

GAOLi, Email: gaoli0519@126.com

Keywords：

Heterotopic abdominal heart transplantation; Animal model; Surgical complication; Learning curve

DOI：

10.7507/1007-4848.20140071

Video：

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Objective To explore the learning process, critical steps and complication prevention of heterotopic abdominal heart transplantation (HAHT) model in rats,and effectively improve the learning process and shorten the learning curve. Methods Surgical experience of 146 rats of HAHT from October 2012 to January 2013 was summarized. Operation time,successful rate and failure reasons were analyzed. Results A training time of 140-150 hours was needed to successfully master surgical skills of HAHT in rats. Average operation time was 83±27 minutes. There were 105 successful HAHT rats (72%) and 41 failed HAHT rats(28%) among 146 HAHT rats. Major failure reasons included hemorrhagic shock (16 rats,39%) grafted heart rebeating failure (7 rats,17%) and anastomotic stenosis (7 rats,17%). Conclusion Vascular anastomosis is the key procedure for the establishment of HAHT model in rats.

Citation： YULei, GAOLi. Critical Steps and Complication Prevention of Heterotopic Abdominal Heart Transplantation in Rats. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2014, 21(2): 249-252. doi: 10.7507/1007-4848.20140071 Copy

1.	Abbott CP,Lindsey ES,Creech O Jr,et al.A technique for heart transplantation in the rat.Arch Surg,1964,89 (10):645-652.
2.	Ono K,Lindsey ES.Improved technique of heart transplantation in rats.J Thorac Cardiovasc Surg,1969,57 (2):225-229.
3.	Niimi M.The technique for heterotopic cardiac transplantation in mice:experience of 3000 operations by one surgeon.J Heart Lung Transplant,2001,20 (10):1123-1128.
4.	吴乃石,张瑶,臧旺福,等.大鼠腹腔心脏移植术后移植物血管病模型的建立.中国胸心血管外科临床杂志,2005,12 (5),332-334.
5.	Dedja A,Dall'Olmo L,Cadrobbi R,et al.Heterotopic cardiac xenotransplantation in rodents:Report of a refined technique in a hamster-to-rat model.Microsurgery,2005,25 (3):227-234.
6.	Ma Y,Wang G.Comparison of 2 heterotopic heart transplant techniques in rats:cervical and abdominal heart.Exp Clin Transplant,2011,9 (2):128-133.
7.	Shan J,Huang Y,Feng L,et al.A modified technique for heterotopic heart transplantation in rats.J Surg Res,2010,164 (1):155-161.
8.	Wen P,Wang X,Wang J,et al.A simple technique for a new working heterotopic heart transplantation model in rats.Transplant Proc,2013,45 (6):2522-2526.
9.	Ruzza A,Vespignani R,Czer LS,et al.Heterotopic heart transplantation in rats:improved anesthetic and surgical technique.Transplant Proc,2010,42 (9):3828-3832.
10.	Wang D,Opelz G,Terness P.A simplified technique for heart transplantation in rats:abdominal vessel branch-sparing and modified venotomy.Microsurgery,2006,26 (6):470-472.
11.	宋光民,张在强,徐巨林,等.套管法与缝合法行大鼠异位心脏移植术的比较.中国胸心血管外科临床杂志,2001,8 (4):288-289.
12.	Uchiyama M,Jin X,Zhang Q,et al.Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells.J Cardiothorac Surg,2012,7:26-33.
13.	Zhang Q,Nakaki T,Iwami D,et al.Induction of regulatory T cells and indefinite survival of fully allogeneic cardiac grafts by ursodeoxycholic acid in mice.Transplantation,2009,88 (12):1360-1370.
14.	Shibutani S,Inoue F,Aramaki O.Effects of immunosuppressants on induction of regulatory cells after intratracheal delivery of alloantigen.Transplantation,2005,79 (8):904-913.
15.	Klupp J,van Gelder T,Dambrin C,et al.Sustained suppression of peripheral blood immune functions by treatment with mycophenolate mofetil correlates with reduced severity of cardiac allograft rejection.J Heart Lung Transplant,2004,23 (3):334-351.
16.	Fang YH,Joo DJ,Lim BJ,et al.The effects of AEB071 (sotrastaurin) with tacrolimus on rat heterotopic cardiac allograft rejection and survival.J Surg Res,2011,171 (1):133-137.
17.	Jin X,Uchiyama M,Zhang Q,et al.Inchingorei-san (TJ-117) and artemisiae capillaris herba induced prolonged survival of fully mismatched cardiac allografts and generated regulatory cells in mice.Evid Based Complement Alternat Med,2012,7 (2):1155-1164.
18.	朱鹏,陈义发,张宜江,等.建立小鼠腹部心脏移植模型的体会.中国普通外科杂志,2007,16 (2):133-135.
19.	刘小孙,张富祥,容松.小鼠腹部异位心脏移植模型制作的改良.中华移植杂志:电子版,2012,6 (1):39-40.
20.	Liu F,Kang SM.Heterotopic heart transplantation in mice.J Vis Exp,2007,(6):238-239.

1. Abbott CP,Lindsey ES,Creech O Jr,et al.A technique for heart transplantation in the rat.Arch Surg,1964,89 (10):645-652.
2. Ono K,Lindsey ES.Improved technique of heart transplantation in rats.J Thorac Cardiovasc Surg,1969,57 (2):225-229.
3. Niimi M.The technique for heterotopic cardiac transplantation in mice:experience of 3000 operations by one surgeon.J Heart Lung Transplant,2001,20 (10):1123-1128.
4. 吴乃石,张瑶,臧旺福,等.大鼠腹腔心脏移植术后移植物血管病模型的建立.中国胸心血管外科临床杂志,2005,12 (5),332-334.
5. Dedja A,Dall'Olmo L,Cadrobbi R,et al.Heterotopic cardiac xenotransplantation in rodents:Report of a refined technique in a hamster-to-rat model.Microsurgery,2005,25 (3):227-234.
6. Ma Y,Wang G.Comparison of 2 heterotopic heart transplant techniques in rats:cervical and abdominal heart.Exp Clin Transplant,2011,9 (2):128-133.
7. Shan J,Huang Y,Feng L,et al.A modified technique for heterotopic heart transplantation in rats.J Surg Res,2010,164 (1):155-161.
8. Wen P,Wang X,Wang J,et al.A simple technique for a new working heterotopic heart transplantation model in rats.Transplant Proc,2013,45 (6):2522-2526.
9. Ruzza A,Vespignani R,Czer LS,et al.Heterotopic heart transplantation in rats:improved anesthetic and surgical technique.Transplant Proc,2010,42 (9):3828-3832.
10. Wang D,Opelz G,Terness P.A simplified technique for heart transplantation in rats:abdominal vessel branch-sparing and modified venotomy.Microsurgery,2006,26 (6):470-472.
11. 宋光民,张在强,徐巨林,等.套管法与缝合法行大鼠异位心脏移植术的比较.中国胸心血管外科临床杂志,2001,8 (4):288-289.
12. Uchiyama M,Jin X,Zhang Q,et al.Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells.J Cardiothorac Surg,2012,7:26-33.
13. Zhang Q,Nakaki T,Iwami D,et al.Induction of regulatory T cells and indefinite survival of fully allogeneic cardiac grafts by ursodeoxycholic acid in mice.Transplantation,2009,88 (12):1360-1370.
14. Shibutani S,Inoue F,Aramaki O.Effects of immunosuppressants on induction of regulatory cells after intratracheal delivery of alloantigen.Transplantation,2005,79 (8):904-913.
15. Klupp J,van Gelder T,Dambrin C,et al.Sustained suppression of peripheral blood immune functions by treatment with mycophenolate mofetil correlates with reduced severity of cardiac allograft rejection.J Heart Lung Transplant,2004,23 (3):334-351.
16. Fang YH,Joo DJ,Lim BJ,et al.The effects of AEB071 (sotrastaurin) with tacrolimus on rat heterotopic cardiac allograft rejection and survival.J Surg Res,2011,171 (1):133-137.
17. Jin X,Uchiyama M,Zhang Q,et al.Inchingorei-san (TJ-117) and artemisiae capillaris herba induced prolonged survival of fully mismatched cardiac allografts and generated regulatory cells in mice.Evid Based Complement Alternat Med,2012,7 (2):1155-1164.
18. 朱鹏,陈义发,张宜江,等.建立小鼠腹部心脏移植模型的体会.中国普通外科杂志,2007,16 (2):133-135.
19. 刘小孙,张富祥,容松.小鼠腹部异位心脏移植模型制作的改良.中华移植杂志:电子版,2012,6 (1):39-40.
20. Liu F,Kang SM.Heterotopic heart transplantation in mice.J Vis Exp,2007,(6):238-239.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Critical Steps and Complication Prevention of Heterotopic Abdominal Heart Transplantation in Rats

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