Effect of Early Coronary Artery Bypass Graft Surgery on Expression Level of Ionophorous Protein at Infracted Border Zone in Dog with Acute Myocardial Infarction_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

LIUHong ¹ , KANHong ² ,  ZHANGWen-feng ¹ , LIShi-jie ¹ , TANGHuai-hao ¹ , LIUZhen-bo ¹ , ZHENGZhi-qiang ¹

1. Department of Cardiothoracic Surgery, The Affiliated Haici Hospital of Medical College, Qingdao University, Qingdao 266033 Shandong, P. R. China;
2. Department of Medical Equipment of The Affiliated Haici Hospital of Medical College, Qingdao University, Qingdao 266033 Shandong, P. R. China;

Corresponding author：

ZHANGWen-feng, Email: zhangwenfeng66@126.com

Keywords：

Myocardial ischemia; Infracted border zone; Sympathetic nervous; Ionophorous protein

DOI：

10.7507/1007-4848.20150195

Video：

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Objective To investigate the effect and significance of early coronary artery bypass graft (CABG) on the expression level of ionophorous protein at infracted border zone (IBZ) in dog with acute myocardial infarction. Methods The anterior descending coronary artery of all thirty healthy mongrel dogs were ligated into myocardial infarction model, whose successful criteria was that the regional myocardium supplied by ligated coronary artery became darker. Coronary artery bypass surgery performed at different time points after myocardial infarction (in the 1st week, the 2nd week, the 4th week, the 6th week respectively) was as an experimental group. While myocardial infarction without coronary artery bypass surgery was set up as a control group. Myocardial tissue without ligation of coronary artery was as a normal group. After 8 weeks, myocardial specimens were cut out in the experimental group and the control group. The local expression levels of ionophorous proteins such as Cav1.2, Kv4.3 and KchIP2 mRNA were detected by means of reverse transcription- polymerase chain reaction (RT-PCR) at normal myocardium and IBZ of the experimental group and the control group. Results Four dogs in every experimental group and all dogs in the control group survived to the end of the study. Three myocardial ion channel proteins expression in the control group were lower than those of the normal group or the experimental group significantly (P<0.01). Cav1.2 mRNA expression in the experimental group in the 4th week or the 6th week was lower than that in the normal group significantly (P<0.05). Kv4.3 and KchIP2 mRNA expression in the experimental group in the 4th week or the 6th week were lower than those in the normal group and the experimental group significantly in the 1st week or the 2nd week (P<0.05). Conclusion Early CABG surgery for acute myocardial infarction could lessen the changes of expression level of ionophorous protein at infracted border zone (IBZ) of dog with acute myocardial infarction. Especially, CABG surgery among two weeks could improve expression level of ionophorous protein, and reduce the effect of ischemia for ionophorous protein and myocardial electrophysiology at IBZ.

Citation： LIUHong, KANHong, ZHANGWen-feng, LIShi-jie, TANGHuai-hao, LIUZhen-bo, ZHENGZhi-qiang. Effect of Early Coronary Artery Bypass Graft Surgery on Expression Level of Ionophorous Protein at Infracted Border Zone in Dog with Acute Myocardial Infarction. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2015, 22(8): 778-782. doi: 10.7507/1007-4848.20150195 Copy

1.	Cao JM, Fishbein MC, Han JB, et al. Relationship between regional cardiac hyperinnervation and ventricular arrhythmia. Circulation, 2000, 101(16):1960-1969.
2.	雷迁, 周成斌. 心肌梗死后交感神经重构防治的研究进展. 岭南心血管病杂志, 2012, 18(6):645-647.
3.	帕力旦·肉孜, 木胡牙提. 心肌梗死后交感神经重构与室性心律失常. 心血管病学进展, 2013, 34(3):371-374.
4.	Tice DA, Zerbino VR, Isom OW, et al. Coronary artery bypass with freeze-preserved saphenous vein allografts. J Thorac Cardiovasc Surg, 1976, 71 (3):378-382.
5.	Chen PS, Chen LS, Cao JM. Sympathetic nerve sprouting, electrical remodeling and the mechanisms of sudden cardiac death. Cardiovasc Res, 2001, 50:409-416.
6.	刘耿星, 方丁芬, 张丽娟. 冠状动脉心脏病患者的心脏自主神经功能性重构浅析. 国际病理科学与临床杂志, 2012, 32(6):508-511.
7.	毛俊, 刘兴鹏, Sunny PO. 心脏自主神经重构与心肌梗死后室性心律失常. 心血管病学进展, 2011, 32(3):305-307.
8.	Aimond F, Alvarez JL, Rauzier JM, et al. Ionic basis of ventricular arrhythmias in remodeled rat heart during long-term myocardial infarction. Cardiovasc Res, 1999, 42:402-415.
9.	王晓明, 石红玲, 崔吉君. 心肌梗死后自主神经功能失调与室性心律失常. 中国循证心血管医学杂志, 2012, 4(4):385-387.
10.	张文峰, 谷天祥, 郑志强, 等. 犬急性心肌梗死早期冠状动脉旁路移植术对梗死周边区心肌神经纤维密度的影响. 中国心血管杂志, 2012, 17(1):38-42.
11.	Nori SL, Gaudino M, Alessandrini F, et al. Immunohistochemical evidence for sympathetic denervation and reinnervation after necrotic injury in rat myocardium. Cell Mol Biol (Noisy-le-grand), 1995, 41:799-807.
12.	Heumann R, Korsching S, Bandtlow C, et al. Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J Cell Biol, 1987, 104:1623-1631.
13.	Dae MW, Lee RJ, Ursell PC, et al. Heterogeneous sympathetic innervation in German shepherd dogs with inherited ventricular arrhythmia and sudden cardiac death. Circulation, 1997, 96:1337-1342.
14.	Cao JM, Chen LS, Ken Knight BH. Nerve sprouting and sudden cardiac death. Circ Res, 2000, 86:816-821.
15.	张涛, 商丽华. 心肌梗死后交感神经重构与室性心律失常. 中国心脏起搏与心电生理杂志, 2012, 26(5):383-385.
16.	Pinto JM, Boyden PA. Electrical remodeling in ischemia an infarction. Cardiovasc Res, 1999, 42(2):284.
17.	张文峰, 谷天祥, 吕英义, 等. 冠状动脉旁路移植术对犬急性心肌梗死心肌神经相关因子表达的影响. 国际外科学杂志, 2011, 38(12):815-819.
18.	张文峰, 谷天祥, 刘泳, 等. 犬急性心肌梗死早期冠状动脉旁路移植术对左室壁运动的影响. 国际外科学杂志, 2013, 40(3):174-178.
19.	Shen MJ, Shinohara T, Park HW, et al. Continuous low-level vagus nerve stimulation reduces stellate ganglion nerve activity and paroxysmal atrial tachyarrhythmias in ambulatory canines. Circulation, 2011, 123(20):2204-2212.
20.	Yang SS, Han W, Cao Y, et al. Effects of high thoracic epidural anesthesia on atrial electrophysiological characteristics and sympathetic nerve sprouting in a canine model of atrial fibrillation. Basic Res Cardiol, 2011, 106(3):495-506.
21.	Yu T, Zhu W, Gu B, et al. Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process. J Appl Physiol, 2012, 113(12):1937-1944.
22.	李荣, 谷天祥, 李卫国, 等. 早期冠状动脉搭桥术对犬急性心肌梗死的范围和肾素系统的影响. 心肺血管病杂志, 2009, 28(1):43-46.
23.	谷天祥, 王春, 张玉海, 等. 非体外循环多支血管病变急性心肌梗死的外科治疗. 中华胸心血管外科杂志, 2006, 22(1):15-17.
24.	Rosati B, Pan Z, Lypen S, et al. Regulation of KChIP2 potassium channel subunit gene expression underlies the gradient of transient outward current in canine and human ventricle. J Physiol, 2001, 533(1):119-125.
25.	董炳庆, 闫素华, 王晔. 糖尿病兔心肌梗死后钾离子通道蛋白基因表达的变化. 中国病理生理杂志, 2011, 27(9):1777-1780.

1. Cao JM, Fishbein MC, Han JB, et al. Relationship between regional cardiac hyperinnervation and ventricular arrhythmia. Circulation, 2000, 101(16):1960-1969.
2. 雷迁, 周成斌. 心肌梗死后交感神经重构防治的研究进展. 岭南心血管病杂志, 2012, 18(6):645-647.
3. 帕力旦·肉孜, 木胡牙提. 心肌梗死后交感神经重构与室性心律失常. 心血管病学进展, 2013, 34(3):371-374.
4. Tice DA, Zerbino VR, Isom OW, et al. Coronary artery bypass with freeze-preserved saphenous vein allografts. J Thorac Cardiovasc Surg, 1976, 71 (3):378-382.
5. Chen PS, Chen LS, Cao JM. Sympathetic nerve sprouting, electrical remodeling and the mechanisms of sudden cardiac death. Cardiovasc Res, 2001, 50:409-416.
6. 刘耿星, 方丁芬, 张丽娟. 冠状动脉心脏病患者的心脏自主神经功能性重构浅析. 国际病理科学与临床杂志, 2012, 32(6):508-511.
7. 毛俊, 刘兴鹏, Sunny PO. 心脏自主神经重构与心肌梗死后室性心律失常. 心血管病学进展, 2011, 32(3):305-307.
8. Aimond F, Alvarez JL, Rauzier JM, et al. Ionic basis of ventricular arrhythmias in remodeled rat heart during long-term myocardial infarction. Cardiovasc Res, 1999, 42:402-415.
9. 王晓明, 石红玲, 崔吉君. 心肌梗死后自主神经功能失调与室性心律失常. 中国循证心血管医学杂志, 2012, 4(4):385-387.
10. 张文峰, 谷天祥, 郑志强, 等. 犬急性心肌梗死早期冠状动脉旁路移植术对梗死周边区心肌神经纤维密度的影响. 中国心血管杂志, 2012, 17(1):38-42.
11. Nori SL, Gaudino M, Alessandrini F, et al. Immunohistochemical evidence for sympathetic denervation and reinnervation after necrotic injury in rat myocardium. Cell Mol Biol (Noisy-le-grand), 1995, 41:799-807.
12. Heumann R, Korsching S, Bandtlow C, et al. Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J Cell Biol, 1987, 104:1623-1631.
13. Dae MW, Lee RJ, Ursell PC, et al. Heterogeneous sympathetic innervation in German shepherd dogs with inherited ventricular arrhythmia and sudden cardiac death. Circulation, 1997, 96:1337-1342.
14. Cao JM, Chen LS, Ken Knight BH. Nerve sprouting and sudden cardiac death. Circ Res, 2000, 86:816-821.
15. 张涛, 商丽华. 心肌梗死后交感神经重构与室性心律失常. 中国心脏起搏与心电生理杂志, 2012, 26(5):383-385.
16. Pinto JM, Boyden PA. Electrical remodeling in ischemia an infarction. Cardiovasc Res, 1999, 42(2):284.
17. 张文峰, 谷天祥, 吕英义, 等. 冠状动脉旁路移植术对犬急性心肌梗死心肌神经相关因子表达的影响. 国际外科学杂志, 2011, 38(12):815-819.
18. 张文峰, 谷天祥, 刘泳, 等. 犬急性心肌梗死早期冠状动脉旁路移植术对左室壁运动的影响. 国际外科学杂志, 2013, 40(3):174-178.
19. Shen MJ, Shinohara T, Park HW, et al. Continuous low-level vagus nerve stimulation reduces stellate ganglion nerve activity and paroxysmal atrial tachyarrhythmias in ambulatory canines. Circulation, 2011, 123(20):2204-2212.
20. Yang SS, Han W, Cao Y, et al. Effects of high thoracic epidural anesthesia on atrial electrophysiological characteristics and sympathetic nerve sprouting in a canine model of atrial fibrillation. Basic Res Cardiol, 2011, 106(3):495-506.
21. Yu T, Zhu W, Gu B, et al. Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process. J Appl Physiol, 2012, 113(12):1937-1944.
22. 李荣, 谷天祥, 李卫国, 等. 早期冠状动脉搭桥术对犬急性心肌梗死的范围和肾素系统的影响. 心肺血管病杂志, 2009, 28(1):43-46.
23. 谷天祥, 王春, 张玉海, 等. 非体外循环多支血管病变急性心肌梗死的外科治疗. 中华胸心血管外科杂志, 2006, 22(1):15-17.
24. Rosati B, Pan Z, Lypen S, et al. Regulation of KChIP2 potassium channel subunit gene expression underlies the gradient of transient outward current in canine and human ventricle. J Physiol, 2001, 533(1):119-125.
25. 董炳庆, 闫素华, 王晔. 糖尿病兔心肌梗死后钾离子通道蛋白基因表达的变化. 中国病理生理杂志, 2011, 27(9):1777-1780.

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Effect of Early Coronary Artery Bypass Graft Surgery on Expression Level of Ionophorous Protein at Infracted Border Zone in Dog with Acute Myocardial Infarction

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