Model Study of Blocking Wnt-1 Signaling Pathway on Non-Small Cell Lung Caner in Nude Mice_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ZHANGFeng-yi ,  LIDing-biao , FANZheng-ning , JIANGYu , LIZheng-hua

Department of Thoracic Surgery, Yanan Hospital, Kunming Medical University, Kunming 650051, P.R.China;

Corresponding author：

LIDing-biao, Email: lidb88@163.com

Keywords：

Wnt signaling pathway; Non-small cell lung cancer inhibition rate; Wnt-1 antibody

DOI：

10.7507/1007-4848.20160144

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the suppression of Wnt-1 pathway on non-small cell lung cancer (NSCLC) by establishing a NSCLC nude mice model of transplanting tumor in Xuanwei county. Methods There were 21 mice with tumor weight from 16-18 g and we divided them into a blank group (n=7), a control group (n=7), and an experiment group (n=7). The blank group were injected with saline, the control group were injected with docetaxel, and the experimental group were injected with Wnt-1 antibody. The mice were executed and the tumor specimens were obtained after six injections. We compared the volumes of the specimens and the inhibition rates of tumor among the three groups. Results There was a statistical difference in volume between the blank group and the experiment group as well as the control group on the 21th and 27th day (P=0.002,P=0.000). The experiment within mice's body showed that both docetaxel and Wnt-1 antibody could inhibit NSCLC from growing, and the inhibition effect of docetaxel was stronger. Conclusion The interdiction of Wnt-1 pathway is functional to restrain the growth of tumor. The docetaxel and Wnt-1 antibody have a positive effect on the treatment of NSCLC.

Citation： ZHANGFeng-yi, LIDing-biao, FANZheng-ning, JIANGYu, LIZheng-hua. Model Study of Blocking Wnt-1 Signaling Pathway on Non-Small Cell Lung Caner in Nude Mice. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2016, 23(6): 608-612. doi: 10.7507/1007-4848.20160144 Copy

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9.	Wang X, Adjei AA. Lung cancer and metastasis:new opportunities and challenges. Cancer & Metastas Rev, 2015, 34(2):1-3.
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12.	Espada J, Calvo MB, Díaz-Prado S, et al. Wnt signalling and cancer stem cells. Clin Transl Oncol, 2009, 11(7):411-427.
13.	Rijsewijk F, Schuermann M, Wagenaar E, et al. The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell, 1987, 50(4):649-657.
14.	Nüsslein-Volhard C, Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature, 1980, 287(5785):795-801.
15.	Wu J, Cohen SM. Repression of Tea shirt marks the initiation of wing development. Development, 2002, 29(10):2411-2418.
16.	Nusse R, Brown A, Papkoff J, et al. A new nomenclature for int-1 and related genes:the Wnt gene family. Cell, 1991, 64(2):231.
17.	Huelsken J, Vogel R, Erdmann B, et al. Beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell, 2001, 105(4):533-545.
18.	Wodarz A, Nusse R. Mechanisms of Wnt signaling in development. Annu Rev Cell Dev Biol, 1998, 14:59-88.
19.	Peifer M, Polakis P. Wnt signaling in oncogenesis and embryogenesis-a look outside the nucleus. Science, 2000, 287(5458):1606-1609.
20.	Curtin JC, Lorenzi MV. Drug discovery approaches to target Wnt signaling in cancer stem cells. Oncotarget, 2010, 1(7):552-566.
21.	Akiri G, Cherian MM, Vijayakumar S, et al. Wnt pathway aberrations including autocrine Wnt activation occur at high frequency in human non-small-cell lungcarcinoma. Oncogene, 2009, 28(21):2163-2172.

1. Jemal A, Siegel R, Xu J, et al. Cancer statistics, 2010. CA Cancer J Clin, 2010, 60(5):277-300.
2. 刘标, 周晓军. 非小细胞肺癌免疫组化标志物专家共识(2014). 临床与实验病理学杂志, 2015(5):481-487.
3. Pallis AG. A Review of treatment in non-small-cell lung cancer. Eur Respir Dis, 2011:174-178.
4. 蔡文科, 石云, 赵青. 哺乳动物雷帕霉素靶蛋白表达与早期非小细胞发预后的关系. 中华肿瘤杂志, 2014, 36(2):120-122.
5. Ettinger DS, Wood DE, Akerley W, et al. Non-small cell lung cancer, versionl 2015. J Nat Compr Cancer Netw Jnccn, 2014, 12(6):1738-1761.
6. 邹德辉, 屈淑贤. 吉西他滨联合紫杉醇治疗晚期非小细胞肺癌的疗效观察. 中国医药指南, 2014(2):71-72.
7. Polakis P. Wnt signaling and cancer. Kaibogaku Zasshi. J Anat, 2009, 84(4):111-112.
8. Nakashima T, Liu D, Nakano J, et al. Wntl over expression associated with tumor proliferation and a poor prognosis in non-small cell lung cancer patients. Oncol Rep, 2008, 19(1):203-209.
9. Wang X, Adjei AA. Lung cancer and metastasis:new opportunities and challenges. Cancer & Metastas Rev, 2015, 34(2):1-3.
10. Hadjihannas MV, Behrens J. CIN By Wnt:growth pathways, mitotic control and chromosomal instability in cancer. Cell ycle, 2006, 5(18):2077-2081.
11. Cadigan KM, Nusse R. Wnt signaling:a common theme in animal development. Genes Dev, 1997, 11(24):3286-3305.
12. Espada J, Calvo MB, Díaz-Prado S, et al. Wnt signalling and cancer stem cells. Clin Transl Oncol, 2009, 11(7):411-427.
13. Rijsewijk F, Schuermann M, Wagenaar E, et al. The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell, 1987, 50(4):649-657.
14. Nüsslein-Volhard C, Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature, 1980, 287(5785):795-801.
15. Wu J, Cohen SM. Repression of Tea shirt marks the initiation of wing development. Development, 2002, 29(10):2411-2418.
16. Nusse R, Brown A, Papkoff J, et al. A new nomenclature for int-1 and related genes:the Wnt gene family. Cell, 1991, 64(2):231.
17. Huelsken J, Vogel R, Erdmann B, et al. Beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell, 2001, 105(4):533-545.
18. Wodarz A, Nusse R. Mechanisms of Wnt signaling in development. Annu Rev Cell Dev Biol, 1998, 14:59-88.
19. Peifer M, Polakis P. Wnt signaling in oncogenesis and embryogenesis-a look outside the nucleus. Science, 2000, 287(5458):1606-1609.
20. Curtin JC, Lorenzi MV. Drug discovery approaches to target Wnt signaling in cancer stem cells. Oncotarget, 2010, 1(7):552-566.
21. Akiri G, Cherian MM, Vijayakumar S, et al. Wnt pathway aberrations including autocrine Wnt activation occur at high frequency in human non-small-cell lungcarcinoma. Oncogene, 2009, 28(21):2163-2172.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Model Study of Blocking Wnt-1 Signaling Pathway on Non-Small Cell Lung Caner in Nude Mice

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