A Mouse Model of Minimally Invasive Pressure Overload-induced Heart Failure Estab-lished through Suprasternal Notch Approach_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ANKang ¹ , LIUXu-guang ² , GAOJian ³ , LVJun-qi ³ ,  ANJun ³

1. Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, P. R. China;
2. Department of Cardiac Surgery, The First Affiliated Hospital of Jilin University, Changchun 130021, P. R. China;
3. Department of Cardiothoracic Surgery, Ningxia Autonomous Region Hospital, The First Affiliated Hospital of Northwest Ethnic University, Yinchuan 750021, P. R. China;

Corresponding author：

ANJun, Email: anjun73@hotmail.com

Keywords：

Pressure overload; Heart failure mode; Aortic arch; Clip; Mouse

DOI：

10.7507/1007-4848.20160257

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To establish a mouse model of pressure overload-induced heart failure via suprasternal notch approach. Methods Male mice were separated into a sham group and an experiment group. Through suprasternal notch approach, the aortic arch port between the origin of the right innominate and left common carotid arteries was partially clipped with tantalum clip, which had a remaining opening of 0.35 mm or 0.25 mm in diameter to cause progres-sively increased afterload. Echocardiography was performed 10 weeks after aortic arch clipped or sham surgery to deter-mine left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular posterior wall end-diastolic thickness (LVPWD), ejection fraction (EF) and fractional shortening (FS). After hemodynamic recordings were completed, mouse body weight (BW) and heart weight (HW) were measured for obtaining HW/BW ratio (mg/g). After heart function examination, mice blood sample was collected for evaluation of serum N-terminal pro-brain natriuretic peptide (NT-proBNP). At the end, part of left ventricular free wall was excised, and hematoxylin and eosin stain was made for histopathological examination. Results The HW/BW, LVEDD and serum NT-proBNP significantly increased in the experiment group compared with those in the sham group (P < 0.01, respectively). The LVPWD, EF and FS significantly decreased compared with the sham group (P < 0.01, respectively). Histopathological examination showed malalignment and rupture of cardiac muscle fibers, hypertrophy and degeneration of myocardial cells, part of which had local or patchy necrosis in left ventricule postoperatively 10 weeks. Conclusion The model of pressure overload-induced heart failure in mice established through suprasternal approach is simple, minimally invasive and reliable.

Citation： ANKang, LIUXu-guang, GAOJian, LVJun-qi, ANJun. A Mouse Model of Minimally Invasive Pressure Overload-induced Heart Failure Estab-lished through Suprasternal Notch Approach. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2016, 23(11): 1099-1102. doi: 10.7507/1007-4848.20160257 Copy

1.	Sysa-Shah P, Sørensen LL, Abraham MR, et al. Electrocardiographic characterization of heart failure in mice that overexpress the ErbB2 receptor tyrosine kinase. Comp Med, 2015, 65(4): 295-307.
2.	Weinberg EO, Schoen FJ, George D, et al. Angiotensin-converting enzyme inhibition prolongs survival and modifies the transition to heart failure in rats with pressure overload hypertrophy due to ascending aortic stenosis. Circulation, 1994, 90(3): 1410-1422.
3.	Li Z, Song Y, Xing R, et al. Heat shock protein 70 acts as a potential biomarker for early diagnosis of heart failure. Plos One, 2013, 8(7): e67964.
4.	Schoner A, Tyrrell C, Wu M, et al. Endocardial endothelial dysfun-ction progressively disrupts initially anti then pro-thrombotic pathways in heart failure mice. Plos One, 2015, 10(11): e0142940.
5.	黄军, 顾晓龙, 董正华, 等.洋地黄对小型猪急性心肌梗死合并急性心力衰竭后左室重塑的影响.中国生化药物杂志, 2015, 35(2): 21-23, 27.
6.	曲辅政, 张晓录, 孙经武, 等.心力衰竭家兔心肌细胞核及肌浆网钙离子/钙调蛋白依赖性蛋白激酶Ⅱ的变化.中国循环杂志, 2011, 30(10): 1000-1003.
7.	王萧, 董浩然, 麦细焕, 等.主动脉瓣关闭不全法建立新西兰兔心衰模型.中国实验动物学报, 2015, 23(2): 127-131.
8.	安君, 李永财, 刘旭光, 等.白介素-17A对压力过负荷心衰小鼠的影响.中国老年学杂志, 2016, 36(3):1046-1047.
9.	Gomes MJ, Martinez PF, Campos DH, et al. Beneficial effects of physical exercise on functional capacity and skeletal muscle oxida-tive stress in rats with aortic stenosis-induced heart failure. Oxid Med Cell Longev, 2016, 2016: 8695716.
10.	Tano N, Kaneko M, Ichihara Y, et al. Allogeneic mesenchymal stromal cells transplanted onto the heart surface achieve therapeutic myocardial repair despite immunologic responses in rats. J Am Heart Assoc, 2016, 5(2): e002815.
11.	Suzuki K, Satoh K, Ikeda S, et al. Basigin promotes cardiac fibrosis and failure in response to chronic pressure overload in mice. Arterioscler Thromb Vasc Biol, 2016, 36(4): 636-646.
12.	An LP, An SK, Wei XH, et al. Atorvastatin improves cardiac func-tion of rats with chronic cardiac failure via inhibiting Rac1/P47phox/ P67phox-mediated ROS release. Eur Rev Med Pharmacol Sci, 2015, 19(20): 3940-3946.
13.	Chen G, Zhao J, Liu C, et al. MG132 proteasome inhibitor upregu-lates the expression of connexin 43 in rats with adriamycin-induced heart failure. Mol Med Rep, 2015, 12(5): 7595-7602.
14.	Westhoff-Bleck M, Kornau F, Haghikia A, et al. NT-proBNT indicates left ventricular impairment and adverse clinical outcome in patients with tetralogy of fallot and pulmonary regurgitation. Can J Cardiol, 2015 Dec 1.pii: S0828-282X(15)01616-5. doi: 10.1016/j.cjca.2015.11.013. [Epub ahead of print].
15.	Jo H, Kim SK, Youn H, et al. A highly sensitive and selective impedi-metric aptasensor for interleukin-17 receptor A. Biosens Bioelectron, 2016, 81: 80-86.
16.	Milovanovic M, Pesic G, Nikolic V, et al. Vitamin D deficiency is associated with increased IL-17 and TNFα levels in patients with chronic heart failure. Arq Bras Cardiol, 2012, 98(3): 259-265.

1. Sysa-Shah P, Sørensen LL, Abraham MR, et al. Electrocardiographic characterization of heart failure in mice that overexpress the ErbB2 receptor tyrosine kinase. Comp Med, 2015, 65(4): 295-307.
2. Weinberg EO, Schoen FJ, George D, et al. Angiotensin-converting enzyme inhibition prolongs survival and modifies the transition to heart failure in rats with pressure overload hypertrophy due to ascending aortic stenosis. Circulation, 1994, 90(3): 1410-1422.
3. Li Z, Song Y, Xing R, et al. Heat shock protein 70 acts as a potential biomarker for early diagnosis of heart failure. Plos One, 2013, 8(7): e67964.
4. Schoner A, Tyrrell C, Wu M, et al. Endocardial endothelial dysfun-ction progressively disrupts initially anti then pro-thrombotic pathways in heart failure mice. Plos One, 2015, 10(11): e0142940.
5. 黄军, 顾晓龙, 董正华, 等.洋地黄对小型猪急性心肌梗死合并急性心力衰竭后左室重塑的影响.中国生化药物杂志, 2015, 35(2): 21-23, 27.
6. 曲辅政, 张晓录, 孙经武, 等.心力衰竭家兔心肌细胞核及肌浆网钙离子/钙调蛋白依赖性蛋白激酶Ⅱ的变化.中国循环杂志, 2011, 30(10): 1000-1003.
7. 王萧, 董浩然, 麦细焕, 等.主动脉瓣关闭不全法建立新西兰兔心衰模型.中国实验动物学报, 2015, 23(2): 127-131.
8. 安君, 李永财, 刘旭光, 等.白介素-17A对压力过负荷心衰小鼠的影响.中国老年学杂志, 2016, 36(3):1046-1047.
9. Gomes MJ, Martinez PF, Campos DH, et al. Beneficial effects of physical exercise on functional capacity and skeletal muscle oxida-tive stress in rats with aortic stenosis-induced heart failure. Oxid Med Cell Longev, 2016, 2016: 8695716.
10. Tano N, Kaneko M, Ichihara Y, et al. Allogeneic mesenchymal stromal cells transplanted onto the heart surface achieve therapeutic myocardial repair despite immunologic responses in rats. J Am Heart Assoc, 2016, 5(2): e002815.
11. Suzuki K, Satoh K, Ikeda S, et al. Basigin promotes cardiac fibrosis and failure in response to chronic pressure overload in mice. Arterioscler Thromb Vasc Biol, 2016, 36(4): 636-646.
12. An LP, An SK, Wei XH, et al. Atorvastatin improves cardiac func-tion of rats with chronic cardiac failure via inhibiting Rac1/P47phox/ P67phox-mediated ROS release. Eur Rev Med Pharmacol Sci, 2015, 19(20): 3940-3946.
13. Chen G, Zhao J, Liu C, et al. MG132 proteasome inhibitor upregu-lates the expression of connexin 43 in rats with adriamycin-induced heart failure. Mol Med Rep, 2015, 12(5): 7595-7602.
14. Westhoff-Bleck M, Kornau F, Haghikia A, et al. NT-proBNT indicates left ventricular impairment and adverse clinical outcome in patients with tetralogy of fallot and pulmonary regurgitation. Can J Cardiol, 2015 Dec 1.pii: S0828-282X(15)01616-5. doi: 10.1016/j.cjca.2015.11.013. [Epub ahead of print].
15. Jo H, Kim SK, Youn H, et al. A highly sensitive and selective impedi-metric aptasensor for interleukin-17 receptor A. Biosens Bioelectron, 2016, 81: 80-86.
16. Milovanovic M, Pesic G, Nikolic V, et al. Vitamin D deficiency is associated with increased IL-17 and TNFα levels in patients with chronic heart failure. Arq Bras Cardiol, 2012, 98(3): 259-265.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

A Mouse Model of Minimally Invasive Pressure Overload-induced Heart Failure Estab-lished through Suprasternal Notch Approach

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