The role of bone marrow-derived endothelial progenitor cells for lung cancer neovascularization_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

 LIU Ruijun ¹ , XIONG Jian ² , CHEN Shufeng ³

1. Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200030, P.R.China;
2. Department of Thoracic Surgery, Shanghai Pudong Hospital, Shanghai, 201209, P.R.China;
3. Fudan University Shanghai Cancer Center, Shanghai, 200032, P.R.China;

Corresponding author：

LIU Ruijun, Email: iamliuruijun@126.com

Keywords：

Endothelial progenitor cells; neovascularization; lung cancer

DOI：

10.7507/1007-4848.201611044

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To observe endothelial progenitor cells (EPCs) participating in the formation of neovascularization in lung adenocarcinoma. Methods EPCs were transfected by recombinant adenovirus carrying LacZ gene in optimal transfection concentration, and then EPCs were injected into animal models of lung adenocarcinoma through the tail vein; afterwards, lung tissues were taken out for pathological examination in the 6^th, 7^th, 8^th week respectively. EPCs were observed to take part in the angiogenesis in the lung adenocarcinoma through X-gal chromogenic dye. Results The optimal multiplicity of infection (MOI) of AD5F35LacZ transfected EPCs was 400. When MOI was 400, maximum transfection efficiency was 97.13±2.08. After 2 weeks, LacZ gene-transfected EPCs began to proliferate in vitro culture, then the EPCs were transplanted into animal models of lung cancer to be involved in the neovascularization formation in the 8^th week after transplantation. Conclusion EPCs are involved in the formation of tumor neovascularization after transplantation.

Citation： LIU Ruijun, XIONG Jian, CHEN Shufeng. The role of bone marrow-derived endothelial progenitor cells for lung cancer neovascularization. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2017, 24(9): 716-720. doi: 10.7507/1007-4848.201611044 Copy

1.	Suzuki K, Sun R, Origuchi M, et al. Mesenchymal stromal cells promote tumor growth through the enhancement of neovascularization. Mol Med, 2011, 17(7-8): 579-587.
2.	Moccia F, Zuccolo E, Poletto V, et al. Endothelial progenitor cells support tumour growth and metastatisation: implications for the resistance to anti-angiogenic therapy. Tumour Biol, 2015, 36 (9): 6603-6614.
3.	柳瑞军, 钟竑, 单根法, 等. 人骨髓内皮祖细胞的分离、诱导培养和扩增. 中国胸心血管外科临床杂志, 2008, 15(2): 113-117.
4.	Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis, Science, 1997, 275(5302): 964-967.
5.	Moschetta M, Mishima Y, Sahin I, et al. Role of endothelial progenitor cells in cancer progression. Biochim Biophys Acta, 2014, 1846(1): 26-39.
6.	Rafii S. Circulating endothelial precursors: mystery, reality, and promise. J Clin Invest, 105(2000): 17-19.
7.	Ribatti D. The involvement of endothelial progenitor cells in tumor angiogenesis. J Cell Mol Med, 2004, 8(3): 294-300.
8.	Nishiwaki Y, Yoshida M, Iwaguro H, et al. Endothelial E-selectin potentiates neovascularization via endothelial progenitor cell-dependent and -independent mechanisms. Arterioscler Thromb Vasc Biol, 2007, 27(3): 512-518.
9.	Pescini F, Cesari F, Giusti B, et al. Bone marrow-derived progenitor cells in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Stroke, 2010, 41(2): 218-223.
10.	Hilbe W, Dirnhofer S, Oberwasserlechner F, et al. CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer. J Clin Pathol, 2004, 57(9): 965-969.
11.	Schoen K, Hirschberg RM, Plendl J, et al. Identification of CD133-, CD34- and KDR-positive cells in the bovine ovary: a new site of vascular wall resident endothelial progenitor cells. Clin Hemorheol Microcirc, 2012, 52(2-4): 67-84.
12.	Matsumoto T, Mifune Y, Kawamoto A, et al. Fracture induced mobilization and incorporation of bone marrow-derived endothelial progenitor cells for bone healing. J Cell Physiol, 2008, 215(1): 234-242.
13.	Kopp HG, Ramos CA, Rafii S. Contribution of endothelial progenitors and proangiogenic hematopoietic cells to vascularization of tumor and ischemic tissue. Curr Opin Hematol, 2006, 13(3): 175-181.
14.	Janic B, Arbab AS. The role and therapeutic potential of endothelial progenitor cells in tumor neovascularization. ScientificWorldJournal, 2010, 10: 1088-1099.
15.	de la Puente P, Muz B, Azab F, et al. Cell trafficking of endothelial progenitor cells in tumor progression. Clin Cancer Res, 2013, 19(13): 3360-3368.
16.	Wang L, Chen L, Wang Q, et al. Circulating endothelial progenitor cells are involved in VEGFR-2-related endothelial differentiation in glioma. Oncol Rep, 2014, 32(5): 2007-2014.
17.	Michiue H, Pastorino S, Yang M, et al. Transdifferentiation of glioblastoma cells into vascular endothelial cells. Proc Natl Acad Sci USA, 2011, 108(11): 4274-4280.

1. Suzuki K, Sun R, Origuchi M, et al. Mesenchymal stromal cells promote tumor growth through the enhancement of neovascularization. Mol Med, 2011, 17(7-8): 579-587.
2. Moccia F, Zuccolo E, Poletto V, et al. Endothelial progenitor cells support tumour growth and metastatisation: implications for the resistance to anti-angiogenic therapy. Tumour Biol, 2015, 36 (9): 6603-6614.
3. 柳瑞军, 钟竑, 单根法, 等. 人骨髓内皮祖细胞的分离、诱导培养和扩增. 中国胸心血管外科临床杂志, 2008, 15(2): 113-117.
4. Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis, Science, 1997, 275(5302): 964-967.
5. Moschetta M, Mishima Y, Sahin I, et al. Role of endothelial progenitor cells in cancer progression. Biochim Biophys Acta, 2014, 1846(1): 26-39.
6. Rafii S. Circulating endothelial precursors: mystery, reality, and promise. J Clin Invest, 105(2000): 17-19.
7. Ribatti D. The involvement of endothelial progenitor cells in tumor angiogenesis. J Cell Mol Med, 2004, 8(3): 294-300.
8. Nishiwaki Y, Yoshida M, Iwaguro H, et al. Endothelial E-selectin potentiates neovascularization via endothelial progenitor cell-dependent and -independent mechanisms. Arterioscler Thromb Vasc Biol, 2007, 27(3): 512-518.
9. Pescini F, Cesari F, Giusti B, et al. Bone marrow-derived progenitor cells in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Stroke, 2010, 41(2): 218-223.
10. Hilbe W, Dirnhofer S, Oberwasserlechner F, et al. CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer. J Clin Pathol, 2004, 57(9): 965-969.
11. Schoen K, Hirschberg RM, Plendl J, et al. Identification of CD133-, CD34- and KDR-positive cells in the bovine ovary: a new site of vascular wall resident endothelial progenitor cells. Clin Hemorheol Microcirc, 2012, 52(2-4): 67-84.
12. Matsumoto T, Mifune Y, Kawamoto A, et al. Fracture induced mobilization and incorporation of bone marrow-derived endothelial progenitor cells for bone healing. J Cell Physiol, 2008, 215(1): 234-242.
13. Kopp HG, Ramos CA, Rafii S. Contribution of endothelial progenitors and proangiogenic hematopoietic cells to vascularization of tumor and ischemic tissue. Curr Opin Hematol, 2006, 13(3): 175-181.
14. Janic B, Arbab AS. The role and therapeutic potential of endothelial progenitor cells in tumor neovascularization. ScientificWorldJournal, 2010, 10: 1088-1099.
15. de la Puente P, Muz B, Azab F, et al. Cell trafficking of endothelial progenitor cells in tumor progression. Clin Cancer Res, 2013, 19(13): 3360-3368.
16. Wang L, Chen L, Wang Q, et al. Circulating endothelial progenitor cells are involved in VEGFR-2-related endothelial differentiation in glioma. Oncol Rep, 2014, 32(5): 2007-2014.
17. Michiue H, Pastorino S, Yang M, et al. Transdifferentiation of glioblastoma cells into vascular endothelial cells. Proc Natl Acad Sci USA, 2011, 108(11): 4274-4280.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

The role of bone marrow-derived endothelial progenitor cells for lung cancer neovascularization

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