LI Jian 1,2,3,4 , LI Wenchao 5 , ZHANG Fengwen 1,2,3,4 , ZHUANG Donglin 1,2,3,4 , WEI Peijian 1,2,3,4 , LI Hang 1,2,3,4 , ZHANG Min 6 , OUYANG Wenbin 1,2,3,4 , PAN Xiangbin 1,2,3,4
  • 1. Department of Structural Heart Disease, National Center for Cardiovascular Disease, Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100037, P. R. China;
  • 2. National Health Commission Key Laboratory of Cardiovascular Regeneration Medicine, Beijing, 100037, P. R. China;
  • 3. Key Laboratory of Innovative Cardiovascular Devices, Chinese Academy of Medical Sciences, Beijing, 100037, P. R. China;
  • 4. National Clinical Research Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing, 100037, P. R. China;
  • 5. Children’s Heart Center, Huazhong Fuwai Hospital, Heart Center, Henan Provincial People’s Hospital, Zhengzhou University People’s Hospital, Zhengzhou, 450000, P. R. China;
  • 6. National Center for Cardiovascular Disease, Animal Experimental Center of Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, Beijing, 102300, P. R. China;
OUYANG Wenbin, Email: droywb31@163.com
Congenital heart disease; patent ductus arteriosus; interventional closure; pulmonary hypertension; animal models
10.7507/1007-4848.202301037
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Objective To explore the method and feasibility of establishing patent ductus arteriosus (PDA) model in Bama miniature pig by using autologous jugular vein, and to provide a large animal model for the development of PDA occluder and the study of pulmonary hypertension associated with congenital heart disease. Methods Five Bama miniature pig weighing about 45 kg were selected to gain the PDA model of the autogenous jugular vein, which was fixed by glutaraldehyde and anastomosed between the ascending aorta and the main pulmonary artery. The patency of PDA was confirmed by echocardiography and angiocardiography immediately and one week after the operation. Two animals were selected to undergo transcatheter closure of PDA via femoral vein 1 week after operation, and the rest were euthanized to obtain PDA and lung tissue for pathological examination. Results The PDA model was successfully established in all five animals with a success rate of 100%. Immediately and 1 week after operation, echocardiography and angiography showed that PDA blood flow was unobstructed, and hematoxylin-eosin staining showed that PDA endothelialization was good. One week after operation, two animals were successfully treated with transcatheter femoral vein occlusion. The pathological examination of lung tissue showed thickening of the intima and muscular layer of pulmonary arterioles, thickening of pulmonary interstitium and infiltration of neutrophils. Conclusion It is safe and feasible to establish a large animal model of PDA by using autogenous jugular vein anastomosis between the ascending aorta and the main pulmonary artery. The model can be used for the development of PDA interventional occlusive devices and the pathophysiological study of congenital heart disease-related pulmonary hypertension.