Expression of MicroRNA-497 in Gastric Cancer and Its Biological Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SUNYe-fei ,  ZHOUJian-ping

Department of Gastrointestinal Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China;

Corresponding author：

ZHOUJian-ping, Email: zjphama@163.com

Keywords：

Gastric cancer; miR-497; BCL2L2; Chemotherapy sensitivity

DOI：

10.7507/1007-9424.20140146

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To detect the expression of microRNA-497(miR-497) in gastric cancer and its biological significance, and to find out its regulatory links with other genes and investigate the effect of miR-497 overexpression interference on chemotherapy sensitivity of gastric cancer cell line. Methods Ninety-four gastric cancer tissues and adjacent to cancer tissues from 2010 to 2013 in this hospital were collected. The expression of miR-497 in the gastric cancer tissue or adjacent to cancer tissue was detected by real-time PCR. SGC-7901 cell line was transfected by miR-497-negative control and miR-497-mimics, respectively. The BCL2L2 expression was measured by Western blot after upregulation of miR-497. The chemotherapy sensitivity of cell line was tested by MTT assay. Results ①The expression of miR-497 was significantly downregulated in the gastric cancer tissues as compared with the adjacent to cancer tissues (P < 0.01). 2 BCL2L2 was predicted to target gene of miR-497. after miR-497 overexpression in cells, the expression of BCL2L2 protein was decreased in the miR-497-mimics cells as compared with negative control cells. The IC₅₀ in the miR-497-mimics cells was significantly lower than that in the negative control cells(P=0.004 6). Conclusions miR-497 expression is frequently downregulated in gastric cancer tissue. Overexpression of miR-497 could inhibit the expression of target gene BCL2L2, and improve chemotherapy sensitivity of gastric cancer cell line, which implies that miR-497 may play an important role in carcinogenesis and therapy of gastric cancer.

Citation： SUNYe-fei, ZHOUJian-ping. Expression of MicroRNA-497 in Gastric Cancer and Its Biological Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(5): 609-613. doi: 10.7507/1007-9424.20140146 Copy

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15.	Navarro SJ, Trinh T, Lucas CA, et al.The C57BL/6J mouse strain background modifies the effect of a mutation in Bcl2l2[J].G3(Bethesda), 2012, 2(1):99-102.
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18.	Baroudi O, Baroudi T, Omrane I, et al.Thymidylate synthase polymorphism in sporadic colorectal and gastric cancer in Tunisian population:a predictive role in 5-fluorouracil based chemotherapy treatment[J].Med Oncol, 2014, 31(2):825.
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20.	Ishigami S, Arigami T, Uenosono Y, et al.Outcomes of advanced gastric cancer patients treated with chemoradiation therapy[J].Gan To Kagaku Ryoho, 2013, 40(6):727-731.

1. Zhu W, Zhu D, Lu S, et al.miR-497 modulates multidrug resistance of human cancer cell lines by targeting BCL2[J].Med Oncol, 2012, 29(1):384-391.
2. Manne U, Shanmugam C, Bovell L, et al.miRNAs as biomarkers for management of patients with colorectal cancer[J].Biomark Med, 2010, 4(5):761-770.
3. Ishida M, Selaru FM.miRNA-based therapeutic strategies[J].Curr Anesthesiol Rep, 2013, 1(1):63-70.
4. Gao M, Yin H, Fei ZW.Clinical application of microRNA in gastric cancer in Eastern Asian area[J].World J Gastroenterol, 2013, 19(13):2019-2027.
5. Furuta M, Kozaki K, Tanimoto K, et al.The tumor-suppressive miR-497-195 cluster targets multiple cell-cycle regulators in hepatocellular carcinoma[J].PLoS One, 2013, 8:e60155.
6. Shen L, Li J, Xu L, et al.miR-497 induces apoptosis of breast cancer cells by targeting bcl-w[J].Exp Ther Med, 2012, 3(3):475-480.
7. Zhao WY, Wang Y, An ZJ, et al.Downregulation of miR-497 promotes tumor growth and angiogenesis by targeting HDGF in non-small cell lung cancer[J].Biochem Biophys Res Commun, 2013, 435(3):466-471.
8. Pan HW, Li SC, Tsai KW.MicroRNA dysregulation in gastric cancer[J].Curr Pharm Des, 2013, 19(7):1273-1284.
9. Shah AA, Leidinger P, Backes C, et al.A set of specific miRNAs is connected with murine and human gastric cancer[J].Genes Chromosomes Cancer, 2013, 52(3):237-249.
10. Hagiwara K, Ochiya T, Kosaka N.A paradigm shift for extracellular vesicles as small RNA carriers:from cellular waste elimination to therapeutic applications[J].Drug Deliv Transl Res, 2014, 4:31-37.
11. Kye MJ, Gonçalves Ido C.The role of miRNA in motor neuron disease[J].Front Cell Neurosci, 2014, 8:15.
12. Cui L, Zhang X, Ye G, et al.Gastric juice microRNAs as potential biomarkers for the screening of gastric cancer[J].Cancer, 2013, 119(9):1618-1626.
13. Luo Q, Li X, Gao Y, et al.MiRNA-497 regulates cell growth and invasion by targeting cyclin E1 in breast cancer[J].Cancer Cell Int, 2013, 13(1):95.
14. Guo ST, Jiang CC, Wang GP, et al.MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer[J].Oncogene, 2013, 32(15):1910-1920.
15. Navarro SJ, Trinh T, Lucas CA, et al.The C57BL/6J mouse strain background modifies the effect of a mutation in Bcl2l2[J].G3(Bethesda), 2012, 2(1):99-102.
16. Kawasaki T, Yokoi S, Tsuda H, et al.BCL2L2 is a probable target for novel 14q11.2 amplification detected in a non-small cell lung cancer cell line[J].Cancer Sci, 2007, 98(7):1070-1077.
17. Zhang YW, Zhang YL, Pan H, et al.Chemotherapy for patients with gastric cancer after complete resection:A network meta-analysis[J].World J Gastroenterol, 2014, 20(2):584-592.
18. Baroudi O, Baroudi T, Omrane I, et al.Thymidylate synthase polymorphism in sporadic colorectal and gastric cancer in Tunisian population:a predictive role in 5-fluorouracil based chemotherapy treatment[J].Med Oncol, 2014, 31(2):825.
19. Ina K, Kataoka T, Ando T.The use of lentinan for treating gastric cancer[J].Anticancer Agents Med Chem, 2013, 13(5):681-688.
20. Ishigami S, Arigami T, Uenosono Y, et al.Outcomes of advanced gastric cancer patients treated with chemoradiation therapy[J].Gan To Kagaku Ryoho, 2013, 40(6):727-731.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of MicroRNA-497 in Gastric Cancer and Its Biological Significance

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