Expressions of MCP-1 in Pancreatic Tissues and Intestinal Mucosa of Severe Acute Pancreatitic Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SHANGXian-hui ¹ , LIJian-guo ² , FANZhen-hai ³ ,  DUIDan-hua ⁴

1. Department of Pediatric, General, Thoracic, and Urinary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China;
2. Department of Gastrointestinal Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China;
3. Key Laboratory of Cell Engineering, Affiliated Hospital of Zunyi Medical Universty, Zunyi 563000, Guizhou Province, China;
4. Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China;

Corresponding author：

DUIDan-hua, Email: ddh1957@163.com

Keywords：

Severe acute pancreatitis; Monocyte chemoattractant protein-1; Interleukin-10; Diamine oxydase; Rat

DOI：

10.7507/1007-9424.20140196

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Objective To observe the effects and mechanism of MCP-1 in ileum and pancreatic tissues in rats with severe acute pancreatitis(SAP). Methods Twenty-fourth healthy SD rats were randomly divided into two groups:control group(n=12) and SAP model group(n=12). SAP was induced in model group by retrograde injection of 3% sodium taucrocholate into the biliopancreatic duct of rats. The control group underwent laparotomy with the manipulation of the intestinal canal. The rats were killed at 12 h and 24 h respectively after operation, blood and tissue samples were collected to detect the indexes as follows:①Expressions of MCP-1 mRNA of pancreatic and ileum tissues were detected by RT-PCR; ②blood plasma MCP-1 and IL-10 levels were detected by ELISA; ③blood plasma AMY and DAO levels were detected by colorimetry; ④the pathological changes of pancreas and ileum tissues were observed. Results Compared with the control group, the levels of MCP-1, IL-10, AMY, and DAO in plasma, pancreas, and ileum tissues were significantly increased in SAP model group(P < 0.01), the expressions of MCP-1 mRNA in pancreas and ileum tissues were up-regulated simultaneously(P < 0.01), and pathological scoring increased obviously(P < 0.01). Conclusion The levels of MCP-1 in plasma, pancreas and ileum tissues are significantly increased in rats with SAP, MCP-1 aggravate the injury of pancreas and ileum tissues.

Citation： SHANGXian-hui, LIJian-guo, FANZhen-hai, DUIDan-hua. Expressions of MCP-1 in Pancreatic Tissues and Intestinal Mucosa of Severe Acute Pancreatitic Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(7): 822-826. doi: 10.7507/1007-9424.20140196 Copy

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2.	黄莛庭.急性胰腺炎细胞内早期事件的再认识[J].中华肝胆外科杂志, 2006, 12(2):75-76.
3.	Ammori BJ. Gut barrier dysfunction in patients with acute pancreatitis[J]. J Hepatobiliary Pancreat Surg, 2002, 9(4):411-412.
4.	王国平. MCP-1及其基因的结构和功能[J].国外医学生理、病理科学与临床分册, 1996, 16(2):67-69.
5.	Rau B, Baumgart K, Krüger CM, et al. CC-chemokine activation in acute pancreatitis:enhanced release of monocyte chemoattractant protein-1 in patients with local and systemic complications[J]. Intensive Care Med, 2003, 29(4):622-629.
6.	Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
7.	Schimidt J, Lewandrowski K, Fernandez-del Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis[J]. Dig Dis Sci, 1992, 37(9):1426-1433.
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9.	王琛, 樊勇.急性胰腺炎常见并发症及防治[J].中国普外基础与临床杂志, 2010, 17(10):1107-1108.
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11.	Pitchumoni CS, Patel NM, Shah P. Factors infl uencing mortality in acute pancreatitis:can we alter them?[J]. J Clin GastroenGastroenterol, 2005, 39(9):798-814.
12.	Bank S, Singh P, Pooran N, et al. Evaluation of factors that have reduced mortality from acute pancreatitis over the past 20 years[J]. J Clin Gastroenterol, 2002, 35(1):50-60.
13.	Jamdar S, Siriwardena AK. Contemporary management of infected necrosis complicating severe acute pancreatitis[J]. Crit Care, 2006, 10(1):101.
14.	刁永鹏, 陈宏, 李非.重症急性胰腺炎肠屏障功能障碍的研究进展[J].中国普外基础与临床杂志, 2009, 16(7):588-592.
15.	Takagi K1, Nakao M, Ogura Y, et al. Sensitive colorimetric assay of serum diamine oxidase[J]. Clin Chim Acta, 1994, 226(1):67-75.
16.	Swank GM, Deitch EA. Role of the gut in multiple organ failure: bacterial translocation and permeability changes[J]. World J Surg, 1996, 20(4):411-417.
17.	吴承堂, 黎沾良, 熊德鑫, 等.急性坏死性胰腺炎时肠道微生态改变与细菌移位的实验研究[J].肝胆胰脾外科杂志, 1996, 2(4):212-214.
18.	吴承堂, 黎沾良.二胺氧化酶在急性坏死性胰腺炎肠道损伤中的作用[J].世界华人消化杂志, 1999, 1(1):35-37.
19.	杨正安, 陆宏伟.大黄对重症急性胰腺炎的治疗价值研究[J].中国急救医学, 2001, 21(12):717.
20.	Raraty MG, Connor S, Criddle DN, et al. Acute pancreatitis and organ failure:pathophysiology, natural history, and management strategies[J]. Curr Gastroenterol Rep, 2004, 6(2):99-103.
21.	Gomez-Cambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the path ophysiology of acute pancreatitis:therapeutical implications[J]. Curr Drug Targets Inflamm Allergy, 2002, 1(4):393-403.
22.	Grady T, Liang P, Ernst SA, et al. Chemokine gene expression in rat pancreatic acinar cells is an early event associated with acute pancreatitis[J]. Gastroenterology, 1997, 113(6):1966-1975.
23.	Brady M, Bhatia M, Christmas S, et al. Expression of the chemokines MCP-1/JE and cytokine-induced neutrophil chemoattractant in early acute pancreatitis[J]. Pancreas, 2002, 25(3): 260-269.
24.	Rongione AJ, Kusske AM, Kwan K, et al. Interleukin-10 reduces the severity of acute pancreatitis in rats[J]. Gastroenterology, 1997, 112(3):960-967.
25.	Menger MD, Plusczyk T, Vollmar B. Microcirculatory derangements in acute pancreatitis[J]. J Hepatobiliary Pancreat Surg, 2001, 8(3):187-194.
26.	廖家智, 王家.美国急性胰腺炎临床指南(诊断部分)[J].临床内科杂志, 2007, 24(2):136-139.

1. Elfar M, Gaber LW, Sabek O, et al. The inflammatory cascade in acute pancreatitis:relevance to clinical disease[J]. Surg Clin North Am, 2007, 87(6):1325-1340.
2. 黄莛庭.急性胰腺炎细胞内早期事件的再认识[J].中华肝胆外科杂志, 2006, 12(2):75-76.
3. Ammori BJ. Gut barrier dysfunction in patients with acute pancreatitis[J]. J Hepatobiliary Pancreat Surg, 2002, 9(4):411-412.
4. 王国平. MCP-1及其基因的结构和功能[J].国外医学生理、病理科学与临床分册, 1996, 16(2):67-69.
5. Rau B, Baumgart K, Krüger CM, et al. CC-chemokine activation in acute pancreatitis:enhanced release of monocyte chemoattractant protein-1 in patients with local and systemic complications[J]. Intensive Care Med, 2003, 29(4):622-629.
6. Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
7. Schimidt J, Lewandrowski K, Fernandez-del Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis[J]. Dig Dis Sci, 1992, 37(9):1426-1433.
8. 孙备, 董承刚, 王刚, 等.重症急性胰腺炎死亡的高危因素分析[J].中华外科杂志, 2007, 45(23):1619-1622.
9. 王琛, 樊勇.急性胰腺炎常见并发症及防治[J].中国普外基础与临床杂志, 2010, 17(10):1107-1108.
10. 闫堃, 黎一鸣, 纪宗正, 等.缓释泵法制备急性胰腺炎大鼠模型[J].中国普外基础与临床杂志, 2011, 18(7):717-721.
11. Pitchumoni CS, Patel NM, Shah P. Factors infl uencing mortality in acute pancreatitis:can we alter them?[J]. J Clin GastroenGastroenterol, 2005, 39(9):798-814.
12. Bank S, Singh P, Pooran N, et al. Evaluation of factors that have reduced mortality from acute pancreatitis over the past 20 years[J]. J Clin Gastroenterol, 2002, 35(1):50-60.
13. Jamdar S, Siriwardena AK. Contemporary management of infected necrosis complicating severe acute pancreatitis[J]. Crit Care, 2006, 10(1):101.
14. 刁永鹏, 陈宏, 李非.重症急性胰腺炎肠屏障功能障碍的研究进展[J].中国普外基础与临床杂志, 2009, 16(7):588-592.
15. Takagi K1, Nakao M, Ogura Y, et al. Sensitive colorimetric assay of serum diamine oxidase[J]. Clin Chim Acta, 1994, 226(1):67-75.
16. Swank GM, Deitch EA. Role of the gut in multiple organ failure: bacterial translocation and permeability changes[J]. World J Surg, 1996, 20(4):411-417.
17. 吴承堂, 黎沾良, 熊德鑫, 等.急性坏死性胰腺炎时肠道微生态改变与细菌移位的实验研究[J].肝胆胰脾外科杂志, 1996, 2(4):212-214.
18. 吴承堂, 黎沾良.二胺氧化酶在急性坏死性胰腺炎肠道损伤中的作用[J].世界华人消化杂志, 1999, 1(1):35-37.
19. 杨正安, 陆宏伟.大黄对重症急性胰腺炎的治疗价值研究[J].中国急救医学, 2001, 21(12):717.
20. Raraty MG, Connor S, Criddle DN, et al. Acute pancreatitis and organ failure:pathophysiology, natural history, and management strategies[J]. Curr Gastroenterol Rep, 2004, 6(2):99-103.
21. Gomez-Cambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the path ophysiology of acute pancreatitis:therapeutical implications[J]. Curr Drug Targets Inflamm Allergy, 2002, 1(4):393-403.
22. Grady T, Liang P, Ernst SA, et al. Chemokine gene expression in rat pancreatic acinar cells is an early event associated with acute pancreatitis[J]. Gastroenterology, 1997, 113(6):1966-1975.
23. Brady M, Bhatia M, Christmas S, et al. Expression of the chemokines MCP-1/JE and cytokine-induced neutrophil chemoattractant in early acute pancreatitis[J]. Pancreas, 2002, 25(3): 260-269.
24. Rongione AJ, Kusske AM, Kwan K, et al. Interleukin-10 reduces the severity of acute pancreatitis in rats[J]. Gastroenterology, 1997, 112(3):960-967.
25. Menger MD, Plusczyk T, Vollmar B. Microcirculatory derangements in acute pancreatitis[J]. J Hepatobiliary Pancreat Surg, 2001, 8(3):187-194.
26. 廖家智, 王家.美国急性胰腺炎临床指南(诊断部分)[J].临床内科杂志, 2007, 24(2):136-139.

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Expressions of MCP-1 in Pancreatic Tissues and Intestinal Mucosa of Severe Acute Pancreatitic Rats

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