Role of FasL Expression in The Apoptosis of Alveolar Macrophages of Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 闫文貌 ,  程石

首都医科大学附属北京天坛医院普通外科（北京100050）;

Corresponding author：

程石, Email: sh_cheng@hotmail.com

Keywords：

重症急性胰腺炎; 肺泡巨噬细胞; FasL蛋白; 凋亡; 氯化钆

DOI：

10.7507/1007-9424.20140205

Video：

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Abstract Full text Figures/Tables Video References Cited by

目的探讨FasL在重症急性胰腺炎（SAP）大鼠肺泡巨噬细胞（AM）凋亡机理中的作用。方法 SD大鼠按数字表法随机分为对照组、SAP组及氯化钆（GdCl₃）组3组，每组16只。SAP模型制成6 h后，经支气管肺泡灌洗获取AM。取右肺下叶行HE染色检查，透射电镜观察和双染色法检测AM凋亡情况，用蛋白免疫印迹法（Western blot法）检测各组AM中FasL蛋白表达水平。结果 GdCl₃组电镜下可见AM典型凋亡形态学特征，AM凋亡率为（22.48±1.44）%，明显高于对照组〔（11.28±1.01）%〕及SAP组〔（6.86±1.35）%〕，其差异有统计学意义（P＜0.05）；GdCl₃组FasL蛋白相对表达量为（1.230±0.041）%，较对照组〔（0.936±0.024）%〕和SAP组〔（0.704±0.011）%〕明显增高（P＜0.05）。AM凋亡率与AM中FasL蛋白表达水平呈线性正相关关系（R²=0.766，P＜0.01）。结论 GdCl₃可能通过激活FasL蛋白表达诱导SAP大鼠AM发生凋亡。

Citation： 闫文貌, 程石. Role of FasL Expression in The Apoptosis of Alveolar Macrophages of Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(7): 869-871. doi: 10.7507/1007-9424.20140205 Copy

1.	Takeda K, Mikami Y, Fukuyama S, et al. Pancreatic ischemia associated with vasospasm in the early phase of human acute necrotizing pancreatitis[J]. Pancreas, 2005, 30(1):40-49.
2.	闫文貌, 程石, 韩颖.氯化钆对重症急性坏死性胰腺炎大鼠肺损伤的防护作用及机制[J].山东医药, 2008, 48(29):19-21.
3.	程石, 何三光, 张佳林.肺泡巨噬细胞活化在急性坏死性胰腺炎大鼠肺损伤中的作用[J].中华外科杂志, 2002, 40(8): 609-612.
4.	中华医学会外科分会胰腺外科学组.重症急性胰腺炎诊治指南[J].中华外科杂志, 2007, 45(11):727-729.
5.	Kaiser AM, Saluja AK, Sengupta A, et al. Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis[J]. Am J Physiol, 1995, 269(5 Pt 1):C1295-C1304.
6.	Saluja A, Hofbauer B, Yamaguchi Y, et al. Induction of apoptosis reduces the severity of caerulein-induced pancreatitis in mice[J]. Biochem Biophys Res Commun, 1996, 220(3):875-878.
7.	张桂信, 陈海龙, 宫爱霞, 等.胰腺腺泡细胞凋亡于急性胰腺炎及治疗策略[J].世界华人消化杂志, 2007, 15(10):1115-1120.
8.	Bhatia M. Apoptosis of pancreatic acinar cells in acute panereatitis:is it good or bad?[J]. J Cell Mol Med, 2004, 8(3):402-409.
9.	程石, 宋茂民, 史敬东.氯化钆对急性坏死性胰腺炎肺泡巨噬细胞分泌炎症介质的影响[J].中国普通外科杂志, 2006, 15(11):836-839.
10.	Mao H, Liu Q, Zhang J, et al. Effects of specific antisense oligonucleotide inhibition of Fas expression on T cell apoptosis induced by Fas ligand[J]. Int Immunopharmacol, 2007, 7(13):1714-1722.
11.	Chang HW, Jeng CR, Lin CM, et al. The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocvte apoptosis invitro[J]. Vet Microbiol, 2007, 122(1-2):72-82.
12.	Uehara S, Gothoh K, Handa H, et al. Immune function in patients with acute pancreatitis[J]. J Gastroenteml Hepatol, 2003, 18(4):363-370.
13.	Kitsmura Y, Hashimoto S, Mizuta N, et al. Fas/FasL-dependent apoptosis of a1veolar cells after lipopolysaccharide-induced lung injury in mice[J]. Am J Respir Crit Care Med, 200l, 163(3 Pt l): 762-769.
14.	Peng Y, Gallagher SF, Haines K, et al. Nuclear factor-kappaB mediates Kupffer cell apoptosis through transcriptional activation of Fas/FasL[J]. J Surg Res, 2006, 130(1):58-65.
15.	Gallagher SF, Peng Y, Haines K, et al. Fas/FasL play a central role in pancreatitis-induced hepatocyte apoptosis[J]. J Gastrointest Surg, 2005, 9(4):467-475.
16.	Gallagher SF, Yang J, Baksh K, et al. Acute pancreatitis induces FasL gene expression and apoptosis in the liver[J]. J Surg Res, 2004, 122(2) :201-209.

1. Takeda K, Mikami Y, Fukuyama S, et al. Pancreatic ischemia associated with vasospasm in the early phase of human acute necrotizing pancreatitis[J]. Pancreas, 2005, 30(1):40-49.
2. 闫文貌, 程石, 韩颖.氯化钆对重症急性坏死性胰腺炎大鼠肺损伤的防护作用及机制[J].山东医药, 2008, 48(29):19-21.
3. 程石, 何三光, 张佳林.肺泡巨噬细胞活化在急性坏死性胰腺炎大鼠肺损伤中的作用[J].中华外科杂志, 2002, 40(8): 609-612.
4. 中华医学会外科分会胰腺外科学组.重症急性胰腺炎诊治指南[J].中华外科杂志, 2007, 45(11):727-729.
5. Kaiser AM, Saluja AK, Sengupta A, et al. Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis[J]. Am J Physiol, 1995, 269(5 Pt 1):C1295-C1304.
6. Saluja A, Hofbauer B, Yamaguchi Y, et al. Induction of apoptosis reduces the severity of caerulein-induced pancreatitis in mice[J]. Biochem Biophys Res Commun, 1996, 220(3):875-878.
7. 张桂信, 陈海龙, 宫爱霞, 等.胰腺腺泡细胞凋亡于急性胰腺炎及治疗策略[J].世界华人消化杂志, 2007, 15(10):1115-1120.
8. Bhatia M. Apoptosis of pancreatic acinar cells in acute panereatitis:is it good or bad?[J]. J Cell Mol Med, 2004, 8(3):402-409.
9. 程石, 宋茂民, 史敬东.氯化钆对急性坏死性胰腺炎肺泡巨噬细胞分泌炎症介质的影响[J].中国普通外科杂志, 2006, 15(11):836-839.
10. Mao H, Liu Q, Zhang J, et al. Effects of specific antisense oligonucleotide inhibition of Fas expression on T cell apoptosis induced by Fas ligand[J]. Int Immunopharmacol, 2007, 7(13):1714-1722.
11. Chang HW, Jeng CR, Lin CM, et al. The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocvte apoptosis invitro[J]. Vet Microbiol, 2007, 122(1-2):72-82.
12. Uehara S, Gothoh K, Handa H, et al. Immune function in patients with acute pancreatitis[J]. J Gastroenteml Hepatol, 2003, 18(4):363-370.
13. Kitsmura Y, Hashimoto S, Mizuta N, et al. Fas/FasL-dependent apoptosis of a1veolar cells after lipopolysaccharide-induced lung injury in mice[J]. Am J Respir Crit Care Med, 200l, 163(3 Pt l): 762-769.
14. Peng Y, Gallagher SF, Haines K, et al. Nuclear factor-kappaB mediates Kupffer cell apoptosis through transcriptional activation of Fas/FasL[J]. J Surg Res, 2006, 130(1):58-65.
15. Gallagher SF, Peng Y, Haines K, et al. Fas/FasL play a central role in pancreatitis-induced hepatocyte apoptosis[J]. J Gastrointest Surg, 2005, 9(4):467-475.
16. Gallagher SF, Yang J, Baksh K, et al. Acute pancreatitis induces FasL gene expression and apoptosis in the liver[J]. J Surg Res, 2004, 122(2) :201-209.

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Role of FasL Expression in The Apoptosis of Alveolar Macrophages of Severe Acute Pancreatitis

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