Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 李俊 ^1,2 , 王健 ¹ , 李鹏飞 ¹ , 韩效帆 ¹ , 朱世春 ¹ , 李广 ¹ ,  张培建 ¹

1. ;
2. ;

Corresponding author：

张培建, Email: yzu.edu.pjz@163.com

Keywords：

间断低氧预适应; 缺血再灌注损伤; 肝脏切除; 超氧化物歧化酶; 丙二醛

DOI：

10.7507/1007-9424.20140305

Video：

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目的探讨间断低氧预适应对大鼠肝大部切除术后残余肝脏合并缺血再灌注引发过氧化损伤的保护作用。方法 78只SD大鼠，用SPSS软件将其随机分为4组：假手术组（SO组，n=6）、肝切除组（PH组，n=24）、肝切除合并缺血再灌注损伤组（IR组，n=24）和间断低氧预适应组（IHP组，n=24）。以无创伤血管夹阻断IR组大鼠入肝血流后切除肝脏的左叶和中叶（约占全肝的70%），20 min后开放入肝血流，残余肝脏发生了缺血再灌注损伤。将IHP组大鼠暴露于10%的低氧环境中，每日持续1 h，连续进行1周，最后1次低氧暴露后行肝切除术（同IR组）。SO组大鼠在术后2 h取材检测，其余各组分别于术后2、6、12及24 h进行检测。检测血清转氨酶（ALT、AST）水平和肝匀浆组织中超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。结果术后2 h，PH组、IR组和IHP组大鼠血清ALT和AST水平均高于SO组（P<0.05）。在术后6、12和24 h，IHP组大鼠血清ALT和AST均高于PH组，但低于IR组（均P<0.05）。与IR组相比，IHP组大鼠术后各时间点残余肝脏中SOD活性明显升高，而MDA含量则显著降低（均P<0.05）。结论间断低氧预适应对残余肝脏缺血再灌注损伤具有保护作用，其机理可能与提高肝脏的抗氧化能力有关。

Citation： 李俊, 王健, 李鹏飞, 韩效帆, 朱世春, 李广, 张培建. Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(10): 1284-1286. doi: 10.7507/1007-9424.20140305 Copy

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4.	Xu WQ, Yu Z, Xie Y, et al. Therapeutic effect of intermittent hypobaric hypoxia on myocardial infarction in rats[J]. Basic Res Cardiol, 2011, 106(3):329-342.
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8.	Choukèr A, Ohta A, Martignoni A, et al. In vivo hypoxic precon ditioning protects from warm liver ischemia-reperfusion injury through the adenosine A2B receptor[J]. Transplantation, 2012, 94(9):894-902.
9.	周斌, 张培建, 李勇, 等. VEGF参与低氧预适应对移植肝肝内胆管上皮细胞的保护作用[J].中国普外基础与临床杂志, 2009, 16(5):365-369.
10.	Elias-Miró M, Jiménez-Castro MB, Rodés J, et al. Current knowledge on oxidative stress in hepatic ischemia/reperfusion[J]. Free Radic Res, 2013, 47(8):555-568.
11.	Klune JR, Tsung A. Molecular biology of liver ischemia/reper-fusion injury:established mechanisms and recent advancements[J]. Surg Clin North Am, 2010, 90(4):665-677.
12.	Cesaratto L, Vascotto C, Calligaris S, et al. The importance of redox state in liver damage[J]. Ann Hepatol, 2004, 3(3):86-92.
13.	Tanrikulu Y, Sahin M, Kismet K, et al. The protective effect of diosmin on hepatic ischemia reperfusion injury:an experimental study[J]. Bosn J Basic Med Sci, 2013, 13(4):218-224.
14.	Yu S, Zheng J, Jiang Z, et al. Protective effect of N-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice[J]. Int J Mol Sci, 2013, 14(9):17680-17693.
15.	梅丹, 徐斌, 罗军, 等.急性重复缺氧对大鼠脑抗氧化活性和脂质过氧化反应的影响[J].中国临床康复, 2003, 7(31):4194-4195.
16.	李洁, 张耀斌.不同低氧训练模式对大鼠力竭运动后骨骼肌线粒体抗氧化能力及呼吸链酶复合体活性的影响[J].生理学报, 2011, 63(1):55-61.
17.	Kislin MS, Tiul'kova EI, Samoĭlov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia[J]. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.

1. Papadopoulos D, Siempis T, Theodorakou E, et al. Hepatic ischemia and reperfusion injury and trauma:current concepts[J]. Arch Trauma Res, 2013, 2(2):63-70.
2. 杨明峰, 张颜波, 孙保亮, 等.低氧预适应小鼠脑匀浆液提取液对大鼠鼠胚海马神经元缺氧复氧后神经细胞的影响[J].中华神经医学杂志, 2009, 8(11):1094-1097.
3. Wang ZH, Chen YX, Zhang CM, et al. Intermittent hypobaric hypoxia improves postischemic recovery of myocardial contractile function via redox signaling during early reperfusion[J]. Am J Physiol Heart Circ Physiol, 2011, 301(4):H1695-H1705.
4. Xu WQ, Yu Z, Xie Y, et al. Therapeutic effect of intermittent hypobaric hypoxia on myocardial infarction in rats[J]. Basic Res Cardiol, 2011, 106(3):329-342.
5. Sasaki H, Fukuda S, Otani H, et al. Hypoxic preconditioning triggers myocardial angiogenesis:a novel approach to enhance contractile functional reserve in rat with myocardial infarction[J]. J Mol Cell Cardiol, 2002, 34(3):335-348.
6. Maslov LN, Lishmanov IuB, Kolar F, et al. Hypoxic preconditioning is a phenomenon increasing tolerance of cardiomyocytes to hypoxia-reoxygenation[J]. Ross Fiziol Zh Im I M Sechenova, 2010, 96(12):1170-1189.
7. Kaminski A, Kasch C, Zhang L, et al. Endothelial nitric oxide synthase mediates protective effects of hypoxic preconditioning in lungs[J]. Respir Physiol Neurobiol, 2007, 155(3):280-285.
8. Choukèr A, Ohta A, Martignoni A, et al. In vivo hypoxic precon ditioning protects from warm liver ischemia-reperfusion injury through the adenosine A2B receptor[J]. Transplantation, 2012, 94(9):894-902.
9. 周斌, 张培建, 李勇, 等. VEGF参与低氧预适应对移植肝肝内胆管上皮细胞的保护作用[J].中国普外基础与临床杂志, 2009, 16(5):365-369.
10. Elias-Miró M, Jiménez-Castro MB, Rodés J, et al. Current knowledge on oxidative stress in hepatic ischemia/reperfusion[J]. Free Radic Res, 2013, 47(8):555-568.
11. Klune JR, Tsung A. Molecular biology of liver ischemia/reper-fusion injury:established mechanisms and recent advancements[J]. Surg Clin North Am, 2010, 90(4):665-677.
12. Cesaratto L, Vascotto C, Calligaris S, et al. The importance of redox state in liver damage[J]. Ann Hepatol, 2004, 3(3):86-92.
13. Tanrikulu Y, Sahin M, Kismet K, et al. The protective effect of diosmin on hepatic ischemia reperfusion injury:an experimental study[J]. Bosn J Basic Med Sci, 2013, 13(4):218-224.
14. Yu S, Zheng J, Jiang Z, et al. Protective effect of N-acetylserotonin against acute hepatic ischemia-reperfusion injury in mice[J]. Int J Mol Sci, 2013, 14(9):17680-17693.
15. 梅丹, 徐斌, 罗军, 等.急性重复缺氧对大鼠脑抗氧化活性和脂质过氧化反应的影响[J].中国临床康复, 2003, 7(31):4194-4195.
16. 李洁, 张耀斌.不同低氧训练模式对大鼠力竭运动后骨骼肌线粒体抗氧化能力及呼吸链酶复合体活性的影响[J].生理学报, 2011, 63(1):55-61.
17. Kislin MS, Tiul'kova EI, Samoĭlov MO. Dynamics of lipid peroxidation of membranes in cells and mitochondrial fraction of neocortex in non-and preconditioned rats after severe hypobaric hypoxia[J]. Zh Evol Biokhim Fiziol, 2011, 47(2):157-164.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways

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