Distribution of Endogenous Salusin-βin Septic Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 XIEFu-jia ¹ , YUEJian-yun ² , ZHANGWen-jing ² , LIBin ¹ , CHAIChen ¹ ,  LUYan ² ,  CAONong ¹

1. Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China;
2. Department of Clinical Laboratory, The First Hospital of Chinese People's Liberation Army, Lanzhou 730030, Gansu Province, China;

Corresponding author：

LUYan, Email: lu73free@gmail.com; CAONong, Email: caonong1955@126.com

Keywords：

Rat; Sepsis; Salusin-β

DOI：

10.7507/1007-9424.20140329

Video：

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Objective To investigate the distribution and content of endogenous salusin-βin septic rats. Methods Thirty-six SPF male SD rats were randomly divided into sham operation group (n=9) and septic model group (n=27).Only the cecum was turn in the sham operation group and the septic model was made by the cecal ligation and puncture (CLP) in the septic model group.The rats were sacrificed at 6 h, 12 h, and 24 h after modeling in the septic model group.The contents of salusin-βin the tissues of spleen, stomach, small intestine, hypothalamus, and serum specimens were detected by enzyme-linked immunosorbent assay. Results ①The salusin-βendogenously generated in the rat tissues including the spleen, stomach, small intestine, hypothalamus, and serum.The content of salusin-βin the spleen tissue was higher than that in the other tissues (P < 0.05).②The contents of salusin-βin the spleen, stomach, small intestine tissues together with the serum increased significantly at 6 h after CLP as compared with the sham operation group (P < 0.05).The contents of salusin-βin the spleen tissue and serum were peaked at 12 h, in the small intestine tissue reached the summit at 24 h.While, the content of salusin-βhad no significant fluctuation in the stomach tissue.The content of salusin-βbegan to increase at 6 h in the hypothalamus tissue, and significantly increased at 12 h after CLP (P < 0.05). Conclusion The time-dependent change of salusin-βin sepsis rats suggests that salusin-βmight be involved with the pathogenesis of sepsis.

Citation： XIEFu-jia, YUEJian-yun, ZHANGWen-jing, LIBin, CHAIChen, LUYan, CAONong. Distribution of Endogenous Salusin-βin Septic Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(11): 1372-1376. doi: 10.7507/1007-9424.20140329 Copy

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2.	Shichiri M, Ishimaru S, Ota T, et al.Salusins:newly identified bioactive peptides with hemodynamic and mitogenic activities[J].Nat Med, 2003, 9(9):1166-1172.
3.	Yang L, Li XY, Zhen YL, et al.The cardiovascular inhibition functions evoked by salusin beta within the caudal ventrolateral medulla are mediated by muscarinic receptors mechanism[J].African Journal of Pharmacy And Pharmacology, 2011, 5(21):2376-2385.
4.	李浩旭, 鲁彦, 刘江静, 等.Salusin-α在尾端延髓腹外侧区的心血管效应机制研究[J].中国药学杂志, 2012(18):1473-1477.
5.	Izumiyama H, Tanaka H, Egi K, et al.Synthetic salusins as cardiac depressors in rat[J].Hypertension, 2005, 45(3):419-425.
6.	Yu F, Zhao J, Yang J, et al.Salusins promote cardiomyocyte growth but does not affect cardiac function in rats[J].Regul Pept, 2004, 122(3):191-197.
7.	Yan XH, Lin L, Pan YX, et al.Salusins protect neonatal rat cardiomyocytes from serum deprivation-induced cell death through upregulation of GRP78[J].J Cardiovasc Pharmacol, 2006, 48(2):41-46.
8.	Masumura M, Watanabe R, Nagashima A, et al.Anti-salusin-βantibody enhances angiogenesis after myocardial ischemia reper fusion injury[J].Expert Opin Ther Targets, 2013, 17(9):1003-1009.
9.	Watanabe T, Nishio K, Kanome T, et al.Impact of salusin-alpha and-beta on human macrophage foam cell formation and coronary atherosclerosis[J].Circulation, 2008, 117(5):638-648.
10.	Watanabe T, Sato K, Itoh F, et al.The roles of salusins in atherosclerosis and related cardiovascular diseases[J].J Am Soc Hypertens, 2011, 5(5):359-365.
11.	Ozgen M, Koca SS, Dagli N, et al.Serum salusin-alpha level in rheumatoid arthritis[J].Regul Pept, 2011, 167(1):125-128.
12.	Koya T, Miyazaki T, Watanabe T, et al.Salusin-βaccelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro[J].Am J Physiol Heart Circ Physiol, 2012, 303(1):H96-H105.
13.	杨磊, 柴琛, 程东升, 等.精氨酸加压素对脓毒症大鼠动脉压力反射的影响[J].中国普通外科杂志, 2011, 20(6):599-604.
14.	乔玮, 柴琛, 杨磊, 等.硫化氢对脓毒症大鼠动脉压力反射的影响[J].中国普外基础与临床杂志, 2011, 18(4):374-379.
15.	Pancoto JA, Corrêa PB, Oliveira-Pelegrin GR, et al.Autonomic dysfunction in experimental sepsis induced by cecal ligation and puncture[J].Auton Neurosci, 2008, 138(1-2):57-63.
16.	Hotchkiss RS, Karl IE.The pathophysiology and treatment of sepsis[J].N Engl J Med, 2003, 348(2):138-150.
17.	Shi KY, Shen FM, Liu AJ, et al.The survival time post-cecal ligation and puncture in sinoaortic denervated rats[J].J Cardiovasc Pharmacol, 2007, 50(2):162-167.
18.	Hildebrand F, Pape HC, Krettek C.The importance of cytokines in the posttraumatic inflammatory reaction[J].Unfallchirurg, 2005, 108(10):793-794, 796-803.
19.	Suzuki N, Shichiri M, Akashi T, et al.Systemic distribution of salusin expression in the rat[J].Hypertens Res, 2007, 30(12):1255-1262.
20.	Suzuki N, Shichiri M, Tateno T, et al.Distinct systemic distribution of salusin-αand salusin-βin the rat[J].Peptides, 2011, 32(4):805-810.
21.	鲁彦, 胡浩.新的心血管活性肽——Salusins研究进展[J].医学综述, 2012, 18(1):4-6.
22.	Sato K, Koyama T, Tateno T, et al.Presence of immunoreactive salusin-alpha in human serum and urine[J].Peptides, 2006, 27(11):2561-2566.
23.	Sato K, Sato T, Susumu T, et al.Presence of immunoreactive salusin-beta in human plasma and urine[J].Regul Pept, 2009, 158(1-3):63-67.
24.	Supowit SC, Katki KA, Hein TW, et al.Vascular reactivity to calcitonin gene-related peptide is enhanced in subtotal nephrectomy-salt induced hypertension[J].Am J Physiol Heart Circ Physiol, 2011, 301(3):H683-H688.
25.	Sukhishvili E, Bekaia G, Kvachadze I.Effect of exogenous calcitonin gene-related peptide on systemic arterial blood pressure in pregnant and non-pregnant rats[J].Georgian Med News, 2011(194):71-75.
26.	Kuroda M, Yoshikawa D, Koizuka S, et al.The effects of isoflurane on adrenomedullin-induced haemodynamic responses in pithed rats[J].Eur J Anaesthesiol, 2008, 25(7):544-549.
27.	Wei X, Zhao C, Jiang J, et al.Adrenomedullin gene delivery alleviates hypertension and its secondary injuries of cardiovascular system[J].Hum Gene Ther, 2005, 16(3):372-380.
28.	Shi JS, Li D, Li N, et al.Inhibition of L-type calcium currents by salusin-beta in rat cardiac ventricular myocytes[J].Peptides, 2010, 31(6):1146-1149.
29.	Ren AJ, Liang Q, Wang R, et al.Effects of salusin-βon action potential and Ionic currents in ventricular myocytes of rats[J].Acta Physiol (Oxf), 2013, 207(1):166-172.
30.	Zhou CH, Liu L, Liu L, et al.Salusin-βnot salusin-αpromotes vascular inflammation in ApoE-deficient mice via the I-κBα/NF-κB pathway[J].PLoS One, 2014, 9(3):e91468.
31.	Sato K, Fujimoto K, Koyama T, et al.Release of salusin-beta from human monocytes/macrophages[J].Regul Pept, 2010, 162(1-3):68-72.
32.	Wei Y, Shan L, Qiao L, et al.Protective effects of huang-lian-jie-du-tang against polymicrobial sepsis induced by cecal ligation and puncture in rats[J].Evid Based Complement Alternat Med, 2013, 2013:909624.

1. Moore LJ, Moore FA.Epidemiology of sepsis in surgical patients[J].Surg Clin North Am, 2012, 92(6):1425-1443.
2. Shichiri M, Ishimaru S, Ota T, et al.Salusins:newly identified bioactive peptides with hemodynamic and mitogenic activities[J].Nat Med, 2003, 9(9):1166-1172.
3. Yang L, Li XY, Zhen YL, et al.The cardiovascular inhibition functions evoked by salusin beta within the caudal ventrolateral medulla are mediated by muscarinic receptors mechanism[J].African Journal of Pharmacy And Pharmacology, 2011, 5(21):2376-2385.
4. 李浩旭, 鲁彦, 刘江静, 等.Salusin-α在尾端延髓腹外侧区的心血管效应机制研究[J].中国药学杂志, 2012(18):1473-1477.
5. Izumiyama H, Tanaka H, Egi K, et al.Synthetic salusins as cardiac depressors in rat[J].Hypertension, 2005, 45(3):419-425.
6. Yu F, Zhao J, Yang J, et al.Salusins promote cardiomyocyte growth but does not affect cardiac function in rats[J].Regul Pept, 2004, 122(3):191-197.
7. Yan XH, Lin L, Pan YX, et al.Salusins protect neonatal rat cardiomyocytes from serum deprivation-induced cell death through upregulation of GRP78[J].J Cardiovasc Pharmacol, 2006, 48(2):41-46.
8. Masumura M, Watanabe R, Nagashima A, et al.Anti-salusin-βantibody enhances angiogenesis after myocardial ischemia reper fusion injury[J].Expert Opin Ther Targets, 2013, 17(9):1003-1009.
9. Watanabe T, Nishio K, Kanome T, et al.Impact of salusin-alpha and-beta on human macrophage foam cell formation and coronary atherosclerosis[J].Circulation, 2008, 117(5):638-648.
10. Watanabe T, Sato K, Itoh F, et al.The roles of salusins in atherosclerosis and related cardiovascular diseases[J].J Am Soc Hypertens, 2011, 5(5):359-365.
11. Ozgen M, Koca SS, Dagli N, et al.Serum salusin-alpha level in rheumatoid arthritis[J].Regul Pept, 2011, 167(1):125-128.
12. Koya T, Miyazaki T, Watanabe T, et al.Salusin-βaccelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro[J].Am J Physiol Heart Circ Physiol, 2012, 303(1):H96-H105.
13. 杨磊, 柴琛, 程东升, 等.精氨酸加压素对脓毒症大鼠动脉压力反射的影响[J].中国普通外科杂志, 2011, 20(6):599-604.
14. 乔玮, 柴琛, 杨磊, 等.硫化氢对脓毒症大鼠动脉压力反射的影响[J].中国普外基础与临床杂志, 2011, 18(4):374-379.
15. Pancoto JA, Corrêa PB, Oliveira-Pelegrin GR, et al.Autonomic dysfunction in experimental sepsis induced by cecal ligation and puncture[J].Auton Neurosci, 2008, 138(1-2):57-63.
16. Hotchkiss RS, Karl IE.The pathophysiology and treatment of sepsis[J].N Engl J Med, 2003, 348(2):138-150.
17. Shi KY, Shen FM, Liu AJ, et al.The survival time post-cecal ligation and puncture in sinoaortic denervated rats[J].J Cardiovasc Pharmacol, 2007, 50(2):162-167.
18. Hildebrand F, Pape HC, Krettek C.The importance of cytokines in the posttraumatic inflammatory reaction[J].Unfallchirurg, 2005, 108(10):793-794, 796-803.
19. Suzuki N, Shichiri M, Akashi T, et al.Systemic distribution of salusin expression in the rat[J].Hypertens Res, 2007, 30(12):1255-1262.
20. Suzuki N, Shichiri M, Tateno T, et al.Distinct systemic distribution of salusin-αand salusin-βin the rat[J].Peptides, 2011, 32(4):805-810.
21. 鲁彦, 胡浩.新的心血管活性肽——Salusins研究进展[J].医学综述, 2012, 18(1):4-6.
22. Sato K, Koyama T, Tateno T, et al.Presence of immunoreactive salusin-alpha in human serum and urine[J].Peptides, 2006, 27(11):2561-2566.
23. Sato K, Sato T, Susumu T, et al.Presence of immunoreactive salusin-beta in human plasma and urine[J].Regul Pept, 2009, 158(1-3):63-67.
24. Supowit SC, Katki KA, Hein TW, et al.Vascular reactivity to calcitonin gene-related peptide is enhanced in subtotal nephrectomy-salt induced hypertension[J].Am J Physiol Heart Circ Physiol, 2011, 301(3):H683-H688.
25. Sukhishvili E, Bekaia G, Kvachadze I.Effect of exogenous calcitonin gene-related peptide on systemic arterial blood pressure in pregnant and non-pregnant rats[J].Georgian Med News, 2011(194):71-75.
26. Kuroda M, Yoshikawa D, Koizuka S, et al.The effects of isoflurane on adrenomedullin-induced haemodynamic responses in pithed rats[J].Eur J Anaesthesiol, 2008, 25(7):544-549.
27. Wei X, Zhao C, Jiang J, et al.Adrenomedullin gene delivery alleviates hypertension and its secondary injuries of cardiovascular system[J].Hum Gene Ther, 2005, 16(3):372-380.
28. Shi JS, Li D, Li N, et al.Inhibition of L-type calcium currents by salusin-beta in rat cardiac ventricular myocytes[J].Peptides, 2010, 31(6):1146-1149.
29. Ren AJ, Liang Q, Wang R, et al.Effects of salusin-βon action potential and Ionic currents in ventricular myocytes of rats[J].Acta Physiol (Oxf), 2013, 207(1):166-172.
30. Zhou CH, Liu L, Liu L, et al.Salusin-βnot salusin-αpromotes vascular inflammation in ApoE-deficient mice via the I-κBα/NF-κB pathway[J].PLoS One, 2014, 9(3):e91468.
31. Sato K, Fujimoto K, Koyama T, et al.Release of salusin-beta from human monocytes/macrophages[J].Regul Pept, 2010, 162(1-3):68-72.
32. Wei Y, Shan L, Qiao L, et al.Protective effects of huang-lian-jie-du-tang against polymicrobial sepsis induced by cecal ligation and puncture in rats[J].Evid Based Complement Alternat Med, 2013, 2013:909624.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Distribution of Endogenous Salusin-βin Septic Rats

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