Protective and Therapeutic Effects of Curcumin Combined with Rhodiola on Rats with Severe Acute Pancreatitis Associated Renal Injury_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIZhi-ling ^1,2 , ZHANGDong ¹ ,  LIUJiang-wei ¹ , WANGHao ¹ , GAOTing ¹ , LUKai-bai ¹ , ZHAOHong-yan ¹

1. Department of Hepatobiliary Surgery, Urumuqi General Hospital of Lanzhou Military Region, Urumuqi 830000, Xinjiang Uygur Autonomous Region, China;
2. Department of General Surgury, The Second People's Hospital of Liangshan Prefecture, Xichang 615000, Sichuan Province, China;

Corresponding author：

LIUJiang-wei, Email: ljw273@sohu.com

Keywords：

Severe acute pancreatitis; Renal injury; Curcumin; Rhodiola; Inducible nitric oxide synthase; Rat

DOI：

10.7507/1007-9424.20140330

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Objective To investigate the effect of Curcumin combined with Rhodiola on rats with severe acute pancreatitis (SAP) associated renal injury and explore the possible mechanisms. Methods A total of 24 rats were randomly divided into SAP with renal injury group (SAP group, n=8), Curcumin group (n=8), Curcumin combined with Rhodiola group (n=8).The SAP group was given 1.5 mL saline through intragastric administration before operation while the Curcumin group was fed with same amount of Curcumin diluent.The Curcumin combined with Rhodiola group was given 1.5 mL Curcumin diluent through intragastric administration and 6 g/kg Rhodiola diluent through intraperitoneal injection before operation.The pancreas and pancreatic tail-segment was dissociated and the head of pancreas were occluded in rats to make the model, blood vessel forceps was loosed after three hours.All the rats were sacrificed at 18 h after modeling.The levels of serum amylase, creatinine, blood urea nitrogen were detected and pathological changes of pancreas and the left kidney were observed under the light microscope.The cell apoptosis was analyzed using TUNEL staining.The serum levels of interleukin (IL)-1β, IL-6, and IL-10 among the three groups were detected by enzyme-linked immunosorbent assay.The expression of inducible nitric oxide synthase (iNOS) mRNA in the right kidney was detected by real-time polymerase chain reaction.The superoxide dismutase (SOD) activity of the renal tissue was determined by hydroxylamine method. Results Compared with the SAP group, the levels of serum amylase, creatinine, blood urea nitrogen, IL-1β, IL-6, the cell apoptosis index, and the expression of iNOS mRNA were significantly decreased, the serum level of IL-10 and the activity of SOD were significantly increased (P < 0.05), the pancreas and the kidney damaged more slightly in the Curcumin group and Curcumin combined with Rhodiola group.Compared with the Curcumin group, the above situations were more better in the Curcumin combined with Rhodiola group. Conclusions Curcumin combined with Rhodiola has a better protective effect on SAP associated renal injury.It might be through inhibiting the expressions of IL-1β, IL-6, stimulating the expression of IL-10, down-regulating the expression of iNOS mRNA, and improving the activity of SOD.It could reduce the cell apoptosis and necrosis of the kidney and improve the ability of the kidney to tolerate hypoxia.

Citation： LIZhi-ling, ZHANGDong, LIUJiang-wei, WANGHao, GAOTing, LUKai-bai, ZHAOHong-yan. Protective and Therapeutic Effects of Curcumin Combined with Rhodiola on Rats with Severe Acute Pancreatitis Associated Renal Injury. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(11): 1377-1383. doi: 10.7507/1007-9424.20140330 Copy

1.	Bennett M, Dent CL, Ma Q, et al.Urine NGAL predicts severity of acute kidney injury after cardiac surgery:a prospective study[J].Clin J Am Soc Nephrol, 2008, 3(3):665-673.
2.	李敏利, 张晓华, 朱人敏, 等.抑制JAK/STAT3信号通路对重症急性胰腺炎肾损伤的保护作用[J].世界华人消化杂志, 2011, 19(32):3297-3301.
3.	张莹, 石承先, 李玉祥, 等.丹参对重症急性胰腺炎大鼠诱导型一氧化氮合成酶mRNA的表达与器官损伤的影响[J].中国中西医结合杂志, 2005, 25(11):1012-1015.
4.	吴新正, 何迎春, 刘红萍, 等.红景天复方对小鼠免疫、耐缺氧和抗疲劳能力的影响[J].湖南中医药大学学报, 2008, 28(1):29-31.
5.	刘江正, 海春旭, 梁欣.红景天醇提物对乙醇诱导QZG细胞氧化损伤的保护作用[J].癌变·畸变·突变, 2011, 23(2):81-86.
6.	查锡良, 周春燕.生物化学[M].第7版.北京:人民卫生出版社, 2008:371-374.
7.	Kozhukhar AV, Yasinska IM, Sumbayev VV.Nitric oxide inhibits HIF-1alpha protein accumulation under hypoxic conditions:implication of 2-oxoglutarate and iron[J].Biochimie, 2006, 88(5):411-418.
8.	Lieberthal W.Biology of ischemic and toxic renal tubular cell injury:role of nitric oxide and the inflammatory response[J].Curr Opin Nephrol Hypertens, 1998, 7(3):289-295.
9.	刘红艳, 王海燕, 叶松, 等.姜黄素药理作用及其机制研究进展[J].中国现代医学杂志, 2012, 22(6):48-51.
10.	刘静, 黄丽, 胡晓松, 等.姜黄素对大鼠重症急性胰腺炎防治作用及其机制[J].中国普通外科杂志, 2012, 21(3):283-289.
11.	王莉, 赵桦.小丛红景天脂溶性成分及抗菌活性分析[J].中国实验方剂学杂志, 2012, 18(16):127-131.
12.	Novovic S, Andersen AM, Ersbøll AK, et al.Proinflammatory cytokines in alcohol or gallstone induced acute pancreatitis[J].JOP, 2009, 10(3):256-262.
13.	王先坤, 詹乐恒, 王世文, 等.IL-10、IL-18在重症急性胰腺炎肺损伤中的作用[J].中国普外基础与临床杂志, 2005, 12(5):443-446.
14.	牛爱丽, 廖青, 张硕, 等.姜黄素对大强度运动大鼠肾损伤的保护作用及其机制[J].湖南师范大学学报:医学版, 2012, 9(1):36-39.
15.	Guan S, He J, Guo W, et al.Adjuvant effects of salidroside from Rhodiola rosea L.on the immune responses to ovalbumin in mice[J].Immunopharmacol Immunotoxicol, 2011, 33(4):738-743.
16.	Lin SS, Chin LW, Chao PC, et al.In vivo Th1 and Th2 cytokine modulation effects of Rhodiola rosea standardised solution and its major constituent, salidroside[J].Phytother Res, 2011, 25(11):1604-1611.
17.	Moore KW, de Waal Malefyt R, Coffman RL, et al.Interleukin-10 and the interleukin-10 receptor[J].Annu Rev Immunol, 2001, 19(2):683-765.
18.	李兆申, 张文俊, 李维勤, 等.胰腺炎[M].北京:科学出版社, 2010:94-95.
19.	Selvam R, Subramanian L, Gayathri R, et al.The anti-oxidant activity of turmeric (Curcuma longa)[J].J Ethnopharmacol, 1995, 47(2):59-67.
20.	龚晓武, 李炳奇, 刘丹丹, 等.红景天黄酮提取及其抗氧化活性研究[J].西北林学院学报, 2011, 26(3):136-138.
21.	许晗, 尹伟伟, 丁鼎.高山红景天总甙抗衰老作用机理的实验研究[J].浙江中医药大学学报, 2011, 35(2):248-249, 252.
22.	金艳玲, 周洋, 王洪海, 等.红景天苷对大鼠肾脏缺血再灌注损伤的预防与保护作用[J].中药新药与临床药理, 2010, 21(1):22-25.
23.	马天翔, 史宁, 陈乾, 等.红景天中8种成分体外抗氧化作用的比较[J].中国药理学通报, 2012, 28(9):1224-1228.
24.	张欣, 隋汝波.红景天对大鼠缺血再灌注后细胞因子的影响[J].中国新药杂志, 2008, 17(6):472-473, 485.
25.	惠远见, 王卫星, 余佳, 等.急性胰腺炎大鼠肾脏中聚腺苷二磷酸核糖聚合酶-1 mRNA的表达及意义[J].中国普外基础与临床杂志, 2010, 17(3):272-274.

1. Bennett M, Dent CL, Ma Q, et al.Urine NGAL predicts severity of acute kidney injury after cardiac surgery:a prospective study[J].Clin J Am Soc Nephrol, 2008, 3(3):665-673.
2. 李敏利, 张晓华, 朱人敏, 等.抑制JAK/STAT3信号通路对重症急性胰腺炎肾损伤的保护作用[J].世界华人消化杂志, 2011, 19(32):3297-3301.
3. 张莹, 石承先, 李玉祥, 等.丹参对重症急性胰腺炎大鼠诱导型一氧化氮合成酶mRNA的表达与器官损伤的影响[J].中国中西医结合杂志, 2005, 25(11):1012-1015.
4. 吴新正, 何迎春, 刘红萍, 等.红景天复方对小鼠免疫、耐缺氧和抗疲劳能力的影响[J].湖南中医药大学学报, 2008, 28(1):29-31.
5. 刘江正, 海春旭, 梁欣.红景天醇提物对乙醇诱导QZG细胞氧化损伤的保护作用[J].癌变·畸变·突变, 2011, 23(2):81-86.
6. 查锡良, 周春燕.生物化学[M].第7版.北京:人民卫生出版社, 2008:371-374.
7. Kozhukhar AV, Yasinska IM, Sumbayev VV.Nitric oxide inhibits HIF-1alpha protein accumulation under hypoxic conditions:implication of 2-oxoglutarate and iron[J].Biochimie, 2006, 88(5):411-418.
8. Lieberthal W.Biology of ischemic and toxic renal tubular cell injury:role of nitric oxide and the inflammatory response[J].Curr Opin Nephrol Hypertens, 1998, 7(3):289-295.
9. 刘红艳, 王海燕, 叶松, 等.姜黄素药理作用及其机制研究进展[J].中国现代医学杂志, 2012, 22(6):48-51.
10. 刘静, 黄丽, 胡晓松, 等.姜黄素对大鼠重症急性胰腺炎防治作用及其机制[J].中国普通外科杂志, 2012, 21(3):283-289.
11. 王莉, 赵桦.小丛红景天脂溶性成分及抗菌活性分析[J].中国实验方剂学杂志, 2012, 18(16):127-131.
12. Novovic S, Andersen AM, Ersbøll AK, et al.Proinflammatory cytokines in alcohol or gallstone induced acute pancreatitis[J].JOP, 2009, 10(3):256-262.
13. 王先坤, 詹乐恒, 王世文, 等.IL-10、IL-18在重症急性胰腺炎肺损伤中的作用[J].中国普外基础与临床杂志, 2005, 12(5):443-446.
14. 牛爱丽, 廖青, 张硕, 等.姜黄素对大强度运动大鼠肾损伤的保护作用及其机制[J].湖南师范大学学报:医学版, 2012, 9(1):36-39.
15. Guan S, He J, Guo W, et al.Adjuvant effects of salidroside from Rhodiola rosea L.on the immune responses to ovalbumin in mice[J].Immunopharmacol Immunotoxicol, 2011, 33(4):738-743.
16. Lin SS, Chin LW, Chao PC, et al.In vivo Th1 and Th2 cytokine modulation effects of Rhodiola rosea standardised solution and its major constituent, salidroside[J].Phytother Res, 2011, 25(11):1604-1611.
17. Moore KW, de Waal Malefyt R, Coffman RL, et al.Interleukin-10 and the interleukin-10 receptor[J].Annu Rev Immunol, 2001, 19(2):683-765.
18. 李兆申, 张文俊, 李维勤, 等.胰腺炎[M].北京:科学出版社, 2010:94-95.
19. Selvam R, Subramanian L, Gayathri R, et al.The anti-oxidant activity of turmeric (Curcuma longa)[J].J Ethnopharmacol, 1995, 47(2):59-67.
20. 龚晓武, 李炳奇, 刘丹丹, 等.红景天黄酮提取及其抗氧化活性研究[J].西北林学院学报, 2011, 26(3):136-138.
21. 许晗, 尹伟伟, 丁鼎.高山红景天总甙抗衰老作用机理的实验研究[J].浙江中医药大学学报, 2011, 35(2):248-249, 252.
22. 金艳玲, 周洋, 王洪海, 等.红景天苷对大鼠肾脏缺血再灌注损伤的预防与保护作用[J].中药新药与临床药理, 2010, 21(1):22-25.
23. 马天翔, 史宁, 陈乾, 等.红景天中8种成分体外抗氧化作用的比较[J].中国药理学通报, 2012, 28(9):1224-1228.
24. 张欣, 隋汝波.红景天对大鼠缺血再灌注后细胞因子的影响[J].中国新药杂志, 2008, 17(6):472-473, 485.
25. 惠远见, 王卫星, 余佳, 等.急性胰腺炎大鼠肾脏中聚腺苷二磷酸核糖聚合酶-1 mRNA的表达及意义[J].中国普外基础与临床杂志, 2010, 17(3):272-274.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective and Therapeutic Effects of Curcumin Combined with Rhodiola on Rats with Severe Acute Pancreatitis Associated Renal Injury

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