Expressions of MVD-CD34 and VEGF in Hepatic Alveolar Hydatid Tissue in Gerbil Model and Their Clinical Significances_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 ZHANGShi-jie ¹ , WUHe-xing ² , LIQi ² , WUXiang-wei ¹ , ZHANGXiao-zhao ² , WANGYan-chao ² , LIANWen-bo ²

1. Department of Hepatobiliary Surgery, The First Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832008, Xinjiang, China;
2. Shihezi University school of Medicine, Shihezi 832008, Xinjiang, China;

Corresponding author：

ZHANGShi-jie, Email: zhangshijiewk1@sina.com

Keywords：

Echioncoccus multilocularis; Angiogenesis; Microvessel density; Vascular endothelial growth factor

DOI：

10.7507/1007-9424.20150038

Video：

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Objective To detect the expressions of microvessel density (MVD)-CD34 and vascular endothelial growth factor (VEGF) in hepatic alveolar hydatid tissue in gerbil model and explore their clinical significances. Methods Sixty health gerbils were randomly equally divided into two groups, an experimental group and a sham operation group, each gerbil was given liver vaccination by opening their abdominal. Each gerbil in the experimental group was injected with approximately 400 echinococcus protoscoleces (0.1 mL), and each gerbil in the sham operation group received a corresponding volume of physiological saline. Six gerbils were sacrificed on day 20, 40, 60, 80, and 100. The hepatic alveolar hydatid tissue (AE) and its surrounding liver tissue (HSAE) were collected from the experimental group and the normal liver tissue (NL) was collected from the sham operation group, and the expressions of MVD-CD34 and VEGF were detected by immunohistochemistry staining (EnVision method). Results Echioncoccus multilocularis hydatid tissues were observed over the liver and in the partly abdominal cavity in the experimental group each gerbil by general observation. The expressions of CD34 and VEGF were observed in the AE at each time point after infection and located in the cytoplasmic of endothelial cells. The number of MVD-CD34 of AE at each time point in the AE was (9.83±3.87)/HP, (25.33±6.71)/HP, (34.50±5.50)/HP, (37.67±5.71)/HP and (44.67±4.93)/HP, respectively, which were significantly higher than those in the HSAE〔0/HP, (1.17±0.98)/HP, (3.50±1.38)/HP, (5.83±2.71)/HP, and(8.83±2.48)/HP, respectively〕and NL (all were 0), P < 0.05. The point of VEGF at each time point in the AE was 2.95±0.46, 3.90±0.68, 4.27±1.05, 5.33±0.95, and 4.50±0.81 respectively, which were significantly higher than those in the HSAE(1.07±0.63, 1.38±0.75, 1.55±0.83, 1.67±0.47, 2.10±0.55, respectively) and NL (1.02±0.83, 1.12±0.63, 1.26±0.26, 1.20±0.74, 1.21±0.28), P < 0.05. Conclusion Angiogenesis might be involved in infiltrated growth of alveococcus, and VEGF might contribute to angiogenesis of alveolar hydatid tissue.

Citation： ZHANGShi-jie, WUHe-xing, LIQi, WUXiang-wei, ZHANGXiao-zhao, WANGYan-chao, LIANWen-bo. Expressions of MVD-CD34 and VEGF in Hepatic Alveolar Hydatid Tissue in Gerbil Model and Their Clinical Significances. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(2): 134-138. doi: 10.7507/1007-9424.20150038 Copy

1.	Piarroux M, Piarroux R, Giorgi R, et al. Clinical features and evolution of alveolar echinococcosis in France from 1982 to 2007: results of a survey in 387 patients. J Hepatol, 2011, 55(5): 1025-1033.
2.	Vuitton DA, Zhang SL, Yang Y, et al. Survival strategy of Echino-coccus multilocularis in the human host. Parasitol Int, 2006: 55 Suppl: S51-S55.
3.	Kademani D, Lewis JT, Lamb DH, et al. Angiogenesis and CD34 expression as a predictor of recurrence in oral squamous cell carcinoma. J Oral Maxillofac Surg, 2009, 67(9): 1800-1805.
4.	Siemerink MJ, Klaassen I, Vogels IM, et al. CD34 marks angiogenic tip cells in human vascular endothelial cell cultures. Angiogenesis, 2012, 15(1): 151-163.
5.	Feng LL, Liu BX, Zhong JY, et al. Effect of grape procyanidins on tumor angiogenesis in liver cancer xenograft models. Asian Pac J Cancer Prev, 2014, 15(2): 737-741.
6.	Moon HS, Park WI, Sung SH, et al. Immunohistochemical and quantitative competitive PCR analyses of midkine and pleiotrophin expression in cervical cancer. Gynecol Oncol, 2003, 88(3): 289-297.
7.	Namisaki T, Yoshiji H, Noguchi R, et al. The vascular endothelial growth factor (VEGF) receptor-2 is a major regulator of VEGF-mediated salvage effect in murine acute hepatic failure. J Angiogenes Res, 2010, 2: 16.
8.	Ferrara N. The role of VEGF in the regulation of physiological and pathological angiogenesis. EXS, 2005, (94): 209-231.
9.	Ferrara N. VEGF-A: a critical regulator of blood vessel growth. Eur Cytokine Netw, 2009, 20(4): 158-163.
10.	Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst, 1992, 84(24): 1875-1887.
11.	Cui XB, Pang XL, Li S, et al. Elevated expression patterns and tight correlation of the PLCE1 and NF-κB signaling in Kazakh patients with esophageal carcinoma. Med Oncol, 2014, 31(1): 791.
12.	Yang P, Yuan W, He J, et al. Overexpression of EphA2, MMP-9, and MVD-CD34 in hepatocellular carcinoma: Implications for tumor progression and prognosis. Hepatol Res, 2009, 39(12): 1169-1177.
13.	Chiang IT, Liu YC, Wang WH, et al. Sorafenib inhibits TPA-induced MMP-9 and VEGF expression via suppression of ERK/NF-κB pathway in hepatocellular carcinoma cells. In Vivo, 2012, 26(4): 671-681.
14.	Takino J, Yamagishi S, Takeuchi M. Glycer-AGEs-RAGE signaling enhances the angiogenic potential of hepatocellular carcinoma by upregulating VEGF expression. World J Gastroenterol, 2012, 18(15): 1781-1788.
15.	李俊, 姚秉礼, 栾梅香, 等.肝泡状棘球蚴病89例的治疗探讨.中华普通外科杂志, 2000, 15(11): 41-42.
16.	荔童, 张示杰.血管新生在肝泡球蚴浸润性生长中的作用.中国人兽共患病学报, 2014, 30(10): 1071-1074.
17.	魏晓丽, 丁剑冰, 许晏, 等.小鼠感染泡球蚴后细胞因子水平的变化.中国寄生虫学与寄生虫病杂志, 2004, 22(6): 361-364.
18.	蒋次鹏.泡球蚴病研究回顾.中国寄生虫病防治杂志, 1998, 11(1): 65-68.
19.	Nagai T, Arao T, Furuta K, et al. Sorafenib inhibits the hepatocyte growth factor-mediated epithelial mesenchymal transition in hepatocellular carcinoma. Mol Cancer Ther, 2011, 10(1): 169-177.
20.	张龙, 张示杰, 曹玉文, 等.骨桥蛋白与肝泡型棘球蚴转移的相关性研究.中国寄生虫学与寄生虫病杂志, 2011, 29(1): 33-36.
21.	Hirama M, Takahashi F, Takahashi K, et al. Osteopontin overproduced by tumor cells acts as a potent angiogenic factor contributing to tumor growth. Cancer Lett, 2003, 198(1): 107-117.
22.	吴何兴, 吴向未, 连文波, 等.抗骨桥蛋白抗体对沙鼠肝多房棘球蚴组织周围血管生成的影响.中国病原生物学杂志, 2014, 9(10): 894-897.
23.	Vuitton DA, Gottstein B. Echinococcus multilocularis and its intermediate host: a model of parasite-host interplay. J Biomed Biotechnol, 2010, 2010: 923193.
24.	Guerret S, Vuitton DA, Liance M, et al. Echinococcus multilocularis: relationship between susceptibility/resistance and liver fibrogenesis in experimental mice. Parasitol Res, 1998, 84(8): 657-667.
25.	王静, 任波, 刘文亚, 等.肝脏泡球蚴病CT灌注成像与微血管密度及血管内皮生长因子的相关性分析.中华放射学杂志, 2011, 45(11): 1036-1039.
26.	McManus DP, Zhang W, Li J, et al. Echinococcosis. Lancet, 2003, 362(9392): 1295-1304.
27.	桑泽杰, 朱帝文, 纪卫政, 等.肝动脉灌注贝伐单抗治疗大鼠肝泡状棘球蚴病后血管内皮生长因子的动态变化.介入放射学杂志, 2014, 23(6): 516-519.

1. Piarroux M, Piarroux R, Giorgi R, et al. Clinical features and evolution of alveolar echinococcosis in France from 1982 to 2007: results of a survey in 387 patients. J Hepatol, 2011, 55(5): 1025-1033.
2. Vuitton DA, Zhang SL, Yang Y, et al. Survival strategy of Echino-coccus multilocularis in the human host. Parasitol Int, 2006: 55 Suppl: S51-S55.
3. Kademani D, Lewis JT, Lamb DH, et al. Angiogenesis and CD34 expression as a predictor of recurrence in oral squamous cell carcinoma. J Oral Maxillofac Surg, 2009, 67(9): 1800-1805.
4. Siemerink MJ, Klaassen I, Vogels IM, et al. CD34 marks angiogenic tip cells in human vascular endothelial cell cultures. Angiogenesis, 2012, 15(1): 151-163.
5. Feng LL, Liu BX, Zhong JY, et al. Effect of grape procyanidins on tumor angiogenesis in liver cancer xenograft models. Asian Pac J Cancer Prev, 2014, 15(2): 737-741.
6. Moon HS, Park WI, Sung SH, et al. Immunohistochemical and quantitative competitive PCR analyses of midkine and pleiotrophin expression in cervical cancer. Gynecol Oncol, 2003, 88(3): 289-297.
7. Namisaki T, Yoshiji H, Noguchi R, et al. The vascular endothelial growth factor (VEGF) receptor-2 is a major regulator of VEGF-mediated salvage effect in murine acute hepatic failure. J Angiogenes Res, 2010, 2: 16.
8. Ferrara N. The role of VEGF in the regulation of physiological and pathological angiogenesis. EXS, 2005, (94): 209-231.
9. Ferrara N. VEGF-A: a critical regulator of blood vessel growth. Eur Cytokine Netw, 2009, 20(4): 158-163.
10. Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst, 1992, 84(24): 1875-1887.
11. Cui XB, Pang XL, Li S, et al. Elevated expression patterns and tight correlation of the PLCE1 and NF-κB signaling in Kazakh patients with esophageal carcinoma. Med Oncol, 2014, 31(1): 791.
12. Yang P, Yuan W, He J, et al. Overexpression of EphA2, MMP-9, and MVD-CD34 in hepatocellular carcinoma: Implications for tumor progression and prognosis. Hepatol Res, 2009, 39(12): 1169-1177.
13. Chiang IT, Liu YC, Wang WH, et al. Sorafenib inhibits TPA-induced MMP-9 and VEGF expression via suppression of ERK/NF-κB pathway in hepatocellular carcinoma cells. In Vivo, 2012, 26(4): 671-681.
14. Takino J, Yamagishi S, Takeuchi M. Glycer-AGEs-RAGE signaling enhances the angiogenic potential of hepatocellular carcinoma by upregulating VEGF expression. World J Gastroenterol, 2012, 18(15): 1781-1788.
15. 李俊, 姚秉礼, 栾梅香, 等.肝泡状棘球蚴病89例的治疗探讨.中华普通外科杂志, 2000, 15(11): 41-42.
16. 荔童, 张示杰.血管新生在肝泡球蚴浸润性生长中的作用.中国人兽共患病学报, 2014, 30(10): 1071-1074.
17. 魏晓丽, 丁剑冰, 许晏, 等.小鼠感染泡球蚴后细胞因子水平的变化.中国寄生虫学与寄生虫病杂志, 2004, 22(6): 361-364.
18. 蒋次鹏.泡球蚴病研究回顾.中国寄生虫病防治杂志, 1998, 11(1): 65-68.
19. Nagai T, Arao T, Furuta K, et al. Sorafenib inhibits the hepatocyte growth factor-mediated epithelial mesenchymal transition in hepatocellular carcinoma. Mol Cancer Ther, 2011, 10(1): 169-177.
20. 张龙, 张示杰, 曹玉文, 等.骨桥蛋白与肝泡型棘球蚴转移的相关性研究.中国寄生虫学与寄生虫病杂志, 2011, 29(1): 33-36.
21. Hirama M, Takahashi F, Takahashi K, et al. Osteopontin overproduced by tumor cells acts as a potent angiogenic factor contributing to tumor growth. Cancer Lett, 2003, 198(1): 107-117.
22. 吴何兴, 吴向未, 连文波, 等.抗骨桥蛋白抗体对沙鼠肝多房棘球蚴组织周围血管生成的影响.中国病原生物学杂志, 2014, 9(10): 894-897.
23. Vuitton DA, Gottstein B. Echinococcus multilocularis and its intermediate host: a model of parasite-host interplay. J Biomed Biotechnol, 2010, 2010: 923193.
24. Guerret S, Vuitton DA, Liance M, et al. Echinococcus multilocularis: relationship between susceptibility/resistance and liver fibrogenesis in experimental mice. Parasitol Res, 1998, 84(8): 657-667.
25. 王静, 任波, 刘文亚, 等.肝脏泡球蚴病CT灌注成像与微血管密度及血管内皮生长因子的相关性分析.中华放射学杂志, 2011, 45(11): 1036-1039.
26. McManus DP, Zhang W, Li J, et al. Echinococcosis. Lancet, 2003, 362(9392): 1295-1304.
27. 桑泽杰, 朱帝文, 纪卫政, 等.肝动脉灌注贝伐单抗治疗大鼠肝泡状棘球蚴病后血管内皮生长因子的动态变化.介入放射学杂志, 2014, 23(6): 516-519.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions of MVD-CD34 and VEGF in Hepatic Alveolar Hydatid Tissue in Gerbil Model and Their Clinical Significances

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