Proliferation and Apoptosis of Karpas299 Cells Induced by Adenovirus-Mediated Interleukin-24 Gene_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 JIANGGuang-bin ¹ ,  LIULei ² , SUNWan-bang ¹ , YANGJi-cheng ³ , GUOJin-jin ¹

1. Graduate School of Zhuhai Campus, Zunyi Medical College, Zhuhai 519041, Guangdong Province, China;
2. Department of General Surgery, Shen Zhen Children's Hospital, Shenzhen 518038, Guangdong Province, China;
3. Department of Immunology, Suzhou University, Suzhou 215021, Jiangsu Province, China;

Corresponding author：

LIULei, Email: liulei3322@aliyun.com

Keywords：

Adenovirus-mediated interleukin-24 gene; Anaplastic large-cell lymphoma; Gene therapy

DOI：

10.7507/1007-9424.20150077

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the proliferation and apoptosis effects of adenovirus-mediated interleukin-24 (Ad-IL-24) gene on Karpas299 cells in vitro. Methods The Karpas299 cells were divided into blank control group, Ad-IL-24 group, and the adenovirus which carrying green fluorescent protein gene group (Ad-GFP group). Karpas299 cells of Ad-IL-24 group were infected by adding 200.0 μL Ad-IL-24, Karpas299 cells of Ad-GFP group were infected by adding 200.0 μL Ad-GFP, but Karpas299 cells of blank control group were treated by adding 200.0 μL PBS. Cells' proliferation inhibition rates of 3 groups were detected by cell counting kit (CCK-8) method at 12, 24, and 48 hours after treatment, respectively, and the cells' apoptosis rates of 3 groups were detected by flow cytometry at 48 hours after treatment. Results Ad-IL-24 can suppress the growth of Karpas299 cells, and the inhibition rate increased over time. Compared with Ad-GFP group at the same time, the cell' proliferation inhibition rate of Ad-IL-24 group was higher at 12, 24, and 48 hours after treatment (P<0.05). In addition, the cells' apoptosis rate of Ad-IL-24 group was higher than those of Ad-GFP group and blank control group at 48 hours after treatment (P<0.05). Conclusion Ad-IL-24 can suppress the growth of Karpas299 cells and induce the apoptosis of it.

Citation： JIANGGuang-bin, LIULei, SUNWan-bang, YANGJi-cheng, GUOJin-jin. Proliferation and Apoptosis of Karpas299 Cells Induced by Adenovirus-Mediated Interleukin-24 Gene. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(3): 284-288. doi: 10.7507/1007-9424.20150077 Copy

1.	蓝必全, 侯梅. 间变性大细胞淋巴瘤治疗的研究进展. 华西医学, 2006, 21(3):623-624.
2.	Le Deley M, Rosolen A, Williams DM, et al. Vinblastine in children andadolescents with high-risk anaplastic large-cell lymphoma:results of the randomized ALCL99-vinblastine trial. J Clin Oncol, 2010, 28(25):3987-3993.
3.	苗慧, 陆凤娟, 翟晓文, 等. 儿童间变型大细胞性非霍奇金淋巴瘤8例临床特点及治疗. 中国小儿血液与肿瘤杂志, 2008, 13(4):161-165.
4.	Dent P, Yacoub A, Hamed HA, et al. MDA-7/IL-24 as a cancer therapeutic:from bench to bedside. Anticancer Drugs, 2010, 21(8):725-731.
5.	汪小华, 叶震敏, 盛伟华, 等. 腺病毒介导hIL-24抑制裸鼠肝癌荷瘤生长及其机制研究. 生物工程学报, 2006, 22(6):925-930.
6.	王晋芬, 王云中, 陈振文, 等. 根据WHO淋巴造血系统肿瘤新分类对山西省淋巴瘤分布特点的分析. 中华病理学杂志, 2006, 35(4):218-223.
7.	皋岚湘, 丁华野, 赵坡, 等. 软组织及骨的小细胞型间变性大细胞淋巴瘤1例报道. 临床与实验病理学杂志, 2000, 16(2):107-110.
8.	胡亚, 杜潇, 廖泉, 等. 原发性胰腺淋巴瘤的诊疗分析. 中国普外基础与临床杂志, 2008, 15(4): 285-286.
9.	Seidemann K, Tiemann M, Schrappe M, et al. Short-pulse B-non-Hodgkin lymphoma-type chemotherapy is efficacious treatment for pediatric anaplastic large cell lymphoma:a report of the Berlin-Frankfurt-Münster Group Trial NHL-BFM 90. Blood, 2001, 97(12):3699-3706.
10.	郑卓军, 汤永民. 恶性血液肿瘤患儿化疗后感染产ESBLs细菌耐药性分析. 中国当代儿科杂志, 2012, 14(7):518-520.
11.	旷凌寒, 江咏梅, 胡正强, 等. 儿科血液病房血培养病原菌分布及耐药情况分析. 中国当代儿科杂志, 2013, 15(4):259-263.
12.	叶启东, 汤静燕, 潘慈, 等. 儿童间变性大细胞淋巴瘤20例临床疗效分析. 中国小儿血液与肿瘤杂志, 2012, 17(1):13-16.
13.	Jiang H, Lin JJ, Su ZZ, et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene, 1995, 11(12):2477-2486.
14.	王少慧, 郑锐青, 孙万邦. mda-7/IL-24及其抗肿瘤机理研究进展. 国际免疫学杂志, 2013, 36(1):40-44.
15.	Caudell EG, Mumm JB, Poindexter N, et al. The protein product of the tumor suppressor gene, melanoma differentiation-associated gene 7, exhibits immunostimulatory activity and is designatedIL-24. J Immunol, 2002, 168(12):6041-6046.
16.	Park MA, Walker T, Martin AP, et al. MDA-7/IL-24-induced cell killing in malignant renal carcinoma cells occurs by a ceramide/CD95/PERK-dependent mechanism. Mol Cancer Ther, 2009, 8(5):1280-1291.
17.	Huang EY, Madireddi MT, Gopalkrishnan RV, et al. Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties. Oncogene, 2001, 20(48):7051-7063.
18.	王从俊, 彭志海, 郑建伟, 等. 携带mda-7基因的复制缺陷型腺病毒通过促进线粒体释放促凋亡蛋白杀伤肝癌细胞. 中华肿瘤杂志, 2008, 30(9):649-651.
19.	Emdad L, Lebedeva IV, Su ZZ, et al. Melanoma differentiation associated gene-7/interleukin-24 reverses multidrug resistance in human colorectal cancer cells. Mol Cancer Ther, 2007, 6(11):2985-2994.
20.	Dash R, Dmitriev I, Su ZZ, et al. Enhanced delivery of mda-7/IL-24 using a serotype chimeric adenovirus (Ad.5/3) improves therapeutic efficacy in low CAR prostate cancer cells. Cancer Gene Ther, 2010, 17(7):447-456.
21.	王少慧, 郭锦锦, 唐艳丽, 等. 重组人白细胞介素-24及其联合顺铂对卵巢癌细胞体外生长的影响. 细胞与分子免疫学杂志, 2014, 30(1):33-35.
22.	郭锦锦, 王少慧, 孙万邦, 等. 重组人IL-24对人肺腺癌A549/DDP细胞增殖活性的影响. 中国免疫学杂志, 2014, 30(9):1178-1181.
23.	郭锦锦, 王少慧, 孙万邦, 等. 重组人IL-24联合顺铂体外抗肺癌细胞生长的实验研究. 免疫学杂志, 2014, 30(9):778-781.
24.	Jia J, Li S, Gong W, et al. mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway. Oncol Rep, 2011, 25(1):195-201.
25.	王少慧, 郭锦锦, 唐艳丽, 等. 多基因遗传表达分析系统检测rhIL-24诱导人卵巢癌细胞凋亡相关基因的应用. 中国免疫学杂志, 2013, 29(12):1312-1316.
26.	Tamai H, Miyake K, Yamaguchi H, et al. AAV8 vector expressing IL24 efficiently suppresses tumor growth mediated by specific mechanisms in MLL/AF4-positive ALL model mice. Blood, 2012, 119(1):64-71.

1. 蓝必全, 侯梅. 间变性大细胞淋巴瘤治疗的研究进展. 华西医学, 2006, 21(3):623-624.
2. Le Deley M, Rosolen A, Williams DM, et al. Vinblastine in children andadolescents with high-risk anaplastic large-cell lymphoma:results of the randomized ALCL99-vinblastine trial. J Clin Oncol, 2010, 28(25):3987-3993.
3. 苗慧, 陆凤娟, 翟晓文, 等. 儿童间变型大细胞性非霍奇金淋巴瘤8例临床特点及治疗. 中国小儿血液与肿瘤杂志, 2008, 13(4):161-165.
4. Dent P, Yacoub A, Hamed HA, et al. MDA-7/IL-24 as a cancer therapeutic:from bench to bedside. Anticancer Drugs, 2010, 21(8):725-731.
5. 汪小华, 叶震敏, 盛伟华, 等. 腺病毒介导hIL-24抑制裸鼠肝癌荷瘤生长及其机制研究. 生物工程学报, 2006, 22(6):925-930.
6. 王晋芬, 王云中, 陈振文, 等. 根据WHO淋巴造血系统肿瘤新分类对山西省淋巴瘤分布特点的分析. 中华病理学杂志, 2006, 35(4):218-223.
7. 皋岚湘, 丁华野, 赵坡, 等. 软组织及骨的小细胞型间变性大细胞淋巴瘤1例报道. 临床与实验病理学杂志, 2000, 16(2):107-110.
8. 胡亚, 杜潇, 廖泉, 等. 原发性胰腺淋巴瘤的诊疗分析. 中国普外基础与临床杂志, 2008, 15(4): 285-286.
9. Seidemann K, Tiemann M, Schrappe M, et al. Short-pulse B-non-Hodgkin lymphoma-type chemotherapy is efficacious treatment for pediatric anaplastic large cell lymphoma:a report of the Berlin-Frankfurt-Münster Group Trial NHL-BFM 90. Blood, 2001, 97(12):3699-3706.
10. 郑卓军, 汤永民. 恶性血液肿瘤患儿化疗后感染产ESBLs细菌耐药性分析. 中国当代儿科杂志, 2012, 14(7):518-520.
11. 旷凌寒, 江咏梅, 胡正强, 等. 儿科血液病房血培养病原菌分布及耐药情况分析. 中国当代儿科杂志, 2013, 15(4):259-263.
12. 叶启东, 汤静燕, 潘慈, 等. 儿童间变性大细胞淋巴瘤20例临床疗效分析. 中国小儿血液与肿瘤杂志, 2012, 17(1):13-16.
13. Jiang H, Lin JJ, Su ZZ, et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene, 1995, 11(12):2477-2486.
14. 王少慧, 郑锐青, 孙万邦. mda-7/IL-24及其抗肿瘤机理研究进展. 国际免疫学杂志, 2013, 36(1):40-44.
15. Caudell EG, Mumm JB, Poindexter N, et al. The protein product of the tumor suppressor gene, melanoma differentiation-associated gene 7, exhibits immunostimulatory activity and is designatedIL-24. J Immunol, 2002, 168(12):6041-6046.
16. Park MA, Walker T, Martin AP, et al. MDA-7/IL-24-induced cell killing in malignant renal carcinoma cells occurs by a ceramide/CD95/PERK-dependent mechanism. Mol Cancer Ther, 2009, 8(5):1280-1291.
17. Huang EY, Madireddi MT, Gopalkrishnan RV, et al. Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties. Oncogene, 2001, 20(48):7051-7063.
18. 王从俊, 彭志海, 郑建伟, 等. 携带mda-7基因的复制缺陷型腺病毒通过促进线粒体释放促凋亡蛋白杀伤肝癌细胞. 中华肿瘤杂志, 2008, 30(9):649-651.
19. Emdad L, Lebedeva IV, Su ZZ, et al. Melanoma differentiation associated gene-7/interleukin-24 reverses multidrug resistance in human colorectal cancer cells. Mol Cancer Ther, 2007, 6(11):2985-2994.
20. Dash R, Dmitriev I, Su ZZ, et al. Enhanced delivery of mda-7/IL-24 using a serotype chimeric adenovirus (Ad.5/3) improves therapeutic efficacy in low CAR prostate cancer cells. Cancer Gene Ther, 2010, 17(7):447-456.
21. 王少慧, 郭锦锦, 唐艳丽, 等. 重组人白细胞介素-24及其联合顺铂对卵巢癌细胞体外生长的影响. 细胞与分子免疫学杂志, 2014, 30(1):33-35.
22. 郭锦锦, 王少慧, 孙万邦, 等. 重组人IL-24对人肺腺癌A549/DDP细胞增殖活性的影响. 中国免疫学杂志, 2014, 30(9):1178-1181.
23. 郭锦锦, 王少慧, 孙万邦, 等. 重组人IL-24联合顺铂体外抗肺癌细胞生长的实验研究. 免疫学杂志, 2014, 30(9):778-781.
24. Jia J, Li S, Gong W, et al. mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway. Oncol Rep, 2011, 25(1):195-201.
25. 王少慧, 郭锦锦, 唐艳丽, 等. 多基因遗传表达分析系统检测rhIL-24诱导人卵巢癌细胞凋亡相关基因的应用. 中国免疫学杂志, 2013, 29(12):1312-1316.
26. Tamai H, Miyake K, Yamaguchi H, et al. AAV8 vector expressing IL24 efficiently suppresses tumor growth mediated by specific mechanisms in MLL/AF4-positive ALL model mice. Blood, 2012, 119(1):64-71.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Proliferation and Apoptosis of Karpas299 Cells Induced by Adenovirus-Mediated Interleukin-24 Gene

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content