Expressions and Significance of E2F1, ID1, and Bax Protein in Gallbladder Adenocarci-noma Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 FENGYing , ZHUJun , TANGFa-bing

Institute of Pathology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China;

Corresponding author：

FENGYing, Email: lzfy12@163.com

Keywords：

Gallbladder adenocarcinoma; Transcriptional factor 1; Inhibitor of diferentiation 1; Bax protein

DOI：

10.7507/1007-9424.20150120

Video：

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Objectives To investigate the expressions and significance of E2F1, ID1, and Bax protein in gallbladder adenocarcinoma tissues. Methods The expressions of E2F1, ID1, and Bax protein in 70 cases of gallbladder adenocarcinoma, 20 cases of high level intraepithelial neoplasia, 30 cases of low level intraepithelial neoplasia, and 20 cases of cholecystitis tissues were tested by using immunohistochemical method. Results The positive expression rates of E2F1, ID1, and Bax protein in gallbladder adenocarcinoma was 84.3%, 70.0%, and 25.7%, respectively; the positive expression rates in high level intraepithelial neoplasia was 75.0%, 65.0%, and 55.0%, respectively; the positive expression rates in low level intraepithelial neoplasia was 16.7%, 23.3%, and 56.7%, respectively; and the positive expression rates in cholecystitis tissues was 10.0%, 20.0%, and 75%, respectively.The positive expression rates of E2F1 and ID1 protein in gallbladder adenocarcinoma were significantly higher than those intraepithelial neoplasia and cholecystitis tissues (P < 0.05), but the positive expression rate of Bax protein in gallbladder adenocarcinoma was lower (P < 0.05).The expressions of E2F1 and ID1 protein were significantly correlated with clinical Nevin staging of gallbladder adenocarcinoma (P < 0.05), but not correlated with the gallbladder adenocarcinoma differentiation degree (P > 0.05).The expression of Bax protein was related to the gallbladder adenocarcinoma differentiation degree (P < 0.05), but not correlated with clinical Nevin staging (P > 0.05).The expression of E2F1 protein was negatively correlated with expression of Bax protein (r=-0.375, P < 0.05), ID1 protein expression has nothing to do with the protein expression of Bax protein (P > 0.05).The expression of E2F1 protein was positively correlated with ID1 protein (r=7.031, P < 0.05). Conclusions The E2F1, ID1, and Bax may play an important role in the generation and development of the gallbladder adenocarcinoma.The combined detection of E2F1, ID1, and Bax have important guiding significance for auxiliary diagnosis and clinical staging of gallbladder adenocarcinoma.

Citation： FENGYing, ZHUJun, TANGFa-bing. Expressions and Significance of E2F1, ID1, and Bax Protein in Gallbladder Adenocarci-noma Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(4): 455-459. doi: 10.7507/1007-9424.20150120 Copy

1.	Lazcano-Ponce EC, Miquel JF, Muñoz N, et al.Epidemiology and molecular pathology of gallbladder cancer.CA Cancer J Clin, 2001, 51(6):349-364.
2.	Biswas AK, Johnson DG.Transcriptional and nontranscriptional functions of E2F1 in response to DNA damage.Cancer Res, 2012, 72(1):13-17.
3.	Cheng YJ, Lee YC, Chiu WC, et al.High Id1 expression, a generally negative prognostic factor, paradoxically predicts a favorable prognosis for adjuvant paclitaxel plus cisplatin therapy in surgically treated lung cancer patients.Oncotarget, 2014, 5(22):11564-11575.
4.	Ling MT, Wang X, Ouyang XS, et al.Id-1 expression promotes cell survival through activation of NF-kappaB signalling pathway in prostate cancer cells.Oncogene, 2003, 22(29):4498-4508.
5.	Rengarajan T, Nandakumar N, Rajendran P, et al.D-pinitol promotes apoptosis in MCF-7 cells via induction of p53 and Bax and inhibition of Bcl-2 and NF-κB.Cancer Prev, 2014, 15(4):1757-1762.
6.	邢传平, 刘斌, 董亮.免疫组织化学标记结果的判断方法.中华病理学杂志, 2001, 30(4):317-318.
7.	毕旭东, 付晓光, 白光.原发性胆囊癌的手术治疗.中国普通外科杂志, 2005, 14(1):68-69.
8.	Zhang K, Dai L, Zhang B, et al.miR-203 is a direct transcriptional target of E2F1 and causes G1 arrest in esophageal cancer cells.J Cell Physiol, 2015, 230(4):903-910.
9.	Yan LH, Wei WY, Cao WL, et al.Overexpression of E2F1 in human gastric adenocarnimo is involved in anti-cancer drug resistance.BMC Cancer, 2014, 14:904.
10.	Shi YJ, Shi H, Troup S, et al.Resveratrol induces apoptosis in breast cancer cells by E2F1 mediated up regulation of ASPP1.Oncol Rep, 2011, 25(6):1713-1719.
11.	Kumari A, Iwasaki T, Pyndiah S, et al.Regulation of E2F1-induced apoptosis by poly (ADP-ribosyl) ation.Cell Death Differ, 2015, 22(2):311-322.
12.	贺荣芳, 谢黎明, 赵强.细胞淋巴瘤中E2F1、BIRC5、PLK1蛋白表达及其临床意义.临床与实验病理学杂志, 2012, 28(3):298-301.
13.	Wood DP.Expression signature of E2F1 and its associated genes predict superficial to invasive progression of bladder tumors.J Urol, 2011, 185(2):469-470.
14.	Vandeputte DA, Troost D, Leenstra S, et al.Expression and distribution of id helix-loop-helix proteins in human astrocytic tumors.Glia, 2002, 38(4):329-338.
15.	Chen KY, Chen CH, TsengYL et al.GCIP functions as a tumor suppressor in non-small cell lung cancer by suppressing Id1-mediated tumor promotion.Oncotarget, 2014, 5(13):5017-5028.
16.	Ma J, Shi M, Li G, et al.Regulation of Id1 expression by epigallocatechin-3-gallate and its effect on the proliferation and apoptosis of poorly differentiated AGS gastric cancer cells.Int J Oncol, 2013, 43(4):1052-1058.
17.	Su Y, Gao L, Teng L, et al.Id1 enhances human ovarian cancer endothelial progenitor cell angiogenesis via PI3K/Akt and NF-κB/MMP-2 signaling pathways.J Transl Med, 2013, 11:132.
18.	Schoppmann SF, Schindl M, Bayer G, et al.Overexpression of Id-1 is associated with poor clinical outcome in node negative breast cancer.Cancer, 2003, 104(6):677-682.
19.	Gupta GP, Perk J, Acharyya S, et al.ID genes mediate tumor reinitiation during breast cancer lung metastasis.Proc Natl Acad Sci U S A, 2007, 104(49):9506-9511.
20.	Lalier L, Cartron PF, Juin P, et al.Bax activation and mitochondrial insertion during apoptosis.Apoptosis, 2007, 12(5):887-896.
21.	张力, 何泽生, 王家林.胆囊癌组织bcl-2和Bax基因表达的意义.第四军医大学学报, 2002, 23(17):1597-1599.
22.	Zhao G, Zhu YL, Colins OE, et al.Activation of the proapoptotic bcl-2 protein Bax by a small molecule induces tumor cell apoptosis.Mol Cell Biol, 2014, 34(7):1198-1207.
23.	Cordara G, Winter HC, Goldstein IJ, et al.The fungal chimerolectin MOA inhibits protein and DNA synthesis in NIH/3T3 cells and may induce BAX-mediated apoptosis.Biochem Biophys Res Commun, 2014, 447(4):586-589.
24.	Jiang H, Zhao PJ, Su D, et al.Paris saponinⅠinduces apoptosis via increasing the Bax/Bcl-2 ratio and caspase-3 expression in gefitinib-resistant non-small cell lung cancer in vitro and in vivo.Mol Med Rep, 2014, 9(6):2265-2272.
25.	Kim JH, Bae J.MCL-1ES induces MCL-1L-dependent BAX-and BAK-independent mitochondrial apoptosis.PLoS One, 2013, 18; 8(11):e79626.

1. Lazcano-Ponce EC, Miquel JF, Muñoz N, et al.Epidemiology and molecular pathology of gallbladder cancer.CA Cancer J Clin, 2001, 51(6):349-364.
2. Biswas AK, Johnson DG.Transcriptional and nontranscriptional functions of E2F1 in response to DNA damage.Cancer Res, 2012, 72(1):13-17.
3. Cheng YJ, Lee YC, Chiu WC, et al.High Id1 expression, a generally negative prognostic factor, paradoxically predicts a favorable prognosis for adjuvant paclitaxel plus cisplatin therapy in surgically treated lung cancer patients.Oncotarget, 2014, 5(22):11564-11575.
4. Ling MT, Wang X, Ouyang XS, et al.Id-1 expression promotes cell survival through activation of NF-kappaB signalling pathway in prostate cancer cells.Oncogene, 2003, 22(29):4498-4508.
5. Rengarajan T, Nandakumar N, Rajendran P, et al.D-pinitol promotes apoptosis in MCF-7 cells via induction of p53 and Bax and inhibition of Bcl-2 and NF-κB.Cancer Prev, 2014, 15(4):1757-1762.
6. 邢传平, 刘斌, 董亮.免疫组织化学标记结果的判断方法.中华病理学杂志, 2001, 30(4):317-318.
7. 毕旭东, 付晓光, 白光.原发性胆囊癌的手术治疗.中国普通外科杂志, 2005, 14(1):68-69.
8. Zhang K, Dai L, Zhang B, et al.miR-203 is a direct transcriptional target of E2F1 and causes G1 arrest in esophageal cancer cells.J Cell Physiol, 2015, 230(4):903-910.
9. Yan LH, Wei WY, Cao WL, et al.Overexpression of E2F1 in human gastric adenocarnimo is involved in anti-cancer drug resistance.BMC Cancer, 2014, 14:904.
10. Shi YJ, Shi H, Troup S, et al.Resveratrol induces apoptosis in breast cancer cells by E2F1 mediated up regulation of ASPP1.Oncol Rep, 2011, 25(6):1713-1719.
11. Kumari A, Iwasaki T, Pyndiah S, et al.Regulation of E2F1-induced apoptosis by poly (ADP-ribosyl) ation.Cell Death Differ, 2015, 22(2):311-322.
12. 贺荣芳, 谢黎明, 赵强.细胞淋巴瘤中E2F1、BIRC5、PLK1蛋白表达及其临床意义.临床与实验病理学杂志, 2012, 28(3):298-301.
13. Wood DP.Expression signature of E2F1 and its associated genes predict superficial to invasive progression of bladder tumors.J Urol, 2011, 185(2):469-470.
14. Vandeputte DA, Troost D, Leenstra S, et al.Expression and distribution of id helix-loop-helix proteins in human astrocytic tumors.Glia, 2002, 38(4):329-338.
15. Chen KY, Chen CH, TsengYL et al.GCIP functions as a tumor suppressor in non-small cell lung cancer by suppressing Id1-mediated tumor promotion.Oncotarget, 2014, 5(13):5017-5028.
16. Ma J, Shi M, Li G, et al.Regulation of Id1 expression by epigallocatechin-3-gallate and its effect on the proliferation and apoptosis of poorly differentiated AGS gastric cancer cells.Int J Oncol, 2013, 43(4):1052-1058.
17. Su Y, Gao L, Teng L, et al.Id1 enhances human ovarian cancer endothelial progenitor cell angiogenesis via PI3K/Akt and NF-κB/MMP-2 signaling pathways.J Transl Med, 2013, 11:132.
18. Schoppmann SF, Schindl M, Bayer G, et al.Overexpression of Id-1 is associated with poor clinical outcome in node negative breast cancer.Cancer, 2003, 104(6):677-682.
19. Gupta GP, Perk J, Acharyya S, et al.ID genes mediate tumor reinitiation during breast cancer lung metastasis.Proc Natl Acad Sci U S A, 2007, 104(49):9506-9511.
20. Lalier L, Cartron PF, Juin P, et al.Bax activation and mitochondrial insertion during apoptosis.Apoptosis, 2007, 12(5):887-896.
21. 张力, 何泽生, 王家林.胆囊癌组织bcl-2和Bax基因表达的意义.第四军医大学学报, 2002, 23(17):1597-1599.
22. Zhao G, Zhu YL, Colins OE, et al.Activation of the proapoptotic bcl-2 protein Bax by a small molecule induces tumor cell apoptosis.Mol Cell Biol, 2014, 34(7):1198-1207.
23. Cordara G, Winter HC, Goldstein IJ, et al.The fungal chimerolectin MOA inhibits protein and DNA synthesis in NIH/3T3 cells and may induce BAX-mediated apoptosis.Biochem Biophys Res Commun, 2014, 447(4):586-589.
24. Jiang H, Zhao PJ, Su D, et al.Paris saponinⅠinduces apoptosis via increasing the Bax/Bcl-2 ratio and caspase-3 expression in gefitinib-resistant non-small cell lung cancer in vitro and in vivo.Mol Med Rep, 2014, 9(6):2265-2272.
25. Kim JH, Bae J.MCL-1ES induces MCL-1L-dependent BAX-and BAK-independent mitochondrial apoptosis.PLoS One, 2013, 18; 8(11):e79626.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions and Significance of E2F1, ID1, and Bax Protein in Gallbladder Adenocarci-noma Tissues

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