Expression of Mitochondrial Transcription Factor A in Colon Cancer and Its Role for Proliferative Regulation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WUKai-ming ,  HEYu-long , LIUFa-keng , CHENJian-hui

Center of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China;

Corresponding author：

HEYu-long, Email: ylh@medmail.com.cn

Keywords：

Mitochondrial transcription factor A; Colon cancer; Proliferation regulation

DOI：

10.7507/1007-9424.20150152

Video：

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Objective To investigate the expression of mitochondrial transcription factor A (TFAM) in colon cancer and the effect of its expression on proliferation of colon cancer cell. Methods Thirty cases of colon cancer in the First Affiliated Hospital of Sun Yat-sen University from March 2013 to April 2013 were studied. TFAM mRNA was detected both in colon cancer tissue and para-cancer tissue by real-time PCR. TFAM mRNA and protein were detected in normal colon cell strain and colon cancer strains SW480, HT-29, and HCT116 by real-time PCR and Western blot, respectively. The proliferation of SW480 cells was evaluated after up-regulating TFAM. Results The expression of TFAM mRNA in the colon cancer tissue was significantly higher than that in the para-cancer tissue (P < 0.000 1). The expressions of TFAM mRNA were obviously increased in the SW480, HT-29, and HCT116 cells as compared with the normal colon cell strain (P value was 0.000 8, 0.002 3, and 0.000 6, respectively), among which the most notable increase was detected in the SW480 cells. The expressions of TFAM protein were obviously increased in the SW480, HT-29, and HCT116 cells as compared with the normal colon cell strain (P value was 0.000 2, 0.003 8, and 0.001 6, respectively), among which the most notable increase was detected in the SW480 cells. After up-regulating TFAM by plasmid transfection, the proliferation of the pcDNA3.1-TFAM-SW480 cell was increased significantly as compared with the pcDNA3.1-SW480 cell at 96 h and 120 h after transfection by the MTT test (P < 0.000 1). The proliferation of the pcDNA3.1-TFAM-SW480 cell was increased significantly as compared with the pcDNA3.1-SW480 cell at 48 h after transfection by the BrdU test (P < 0.001 0). Conclusion TFAM expression is high in colon cancer. Up-regulated TFAM could promote the proliferation of colon cancer cells.

Citation： WUKai-ming, HEYu-long, LIUFa-keng, CHENJian-hui. Expression of Mitochondrial Transcription Factor A in Colon Cancer and Its Role for Proliferative Regulation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(5): 576-580. doi: 10.7507/1007-9424.20150152 Copy

1.	Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide:sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer, 2015, 136(5):E359-E386.
2.	Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin, 2011, 61(2):69-90.
3.	Broussard EK, Disis ML. TNM staging in colorectal cancer:T is for T cell and M is for memory. J Clin Oncol, 2011, 29(6):601-603.
4.	de la Cruz-Merino L, Henao Carrasco F, Vicente Baz D, et al. Immune microenvironment in colorectal cancer:a new hallmark to change old paradigms. Clin Dev Immunol, 2011, 2011:174149.
5.	Lao VV, Grady WM. Epigenetics and colorectal cancer. Nat Rev Gastroenterol Hepatol, 2011, 8(12):686-700.
6.	Mlecnik B, Tosolini M, Kirilovsky A, et al. Histopathologic-based prognostic factors of colorectal cancers are associated with the state of the local immune reaction. J Clin Oncol, 2011, 29(6):610-618.
7.	Baffy G. Uncoupling protein-2 and cancer. Mitochondrion, 2010, 10(3):243-252.
8.	Litonin D, Sologub M, Shi Y, et al. Human mitochondrial transcription revisited:only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. J Biol Chem, 2010, 285(24):18129-18133.
9.	Mayevsky A. Mitochondrial function and energy metabolism in cancer cells:past overview and future perspectives. Mitochondrion, 2009, 9(3):165-179.
10.	Toki N, Kagami S, Kurita T, et al. Expression of mitochondrial transcription factor A in endometrial carcinomas:clinicopathologic correlations and prognostic significance. Virchows Arch, 2010, 456(4):387-393.
11.	Wen Z, Lei Z, Jin-An M, et al. The inhibitory role of miR-214 in cervical cancer cells through directly targeting mitochondrial transcription factor A (TFAM). Eur J Gynaecol Oncol, 2014, 35(6):676-682.
12.	Yao J, Zhou E, Wang Y, et al. microRNA-200a inhibits cell proliferation by targeting mitochondrial transcription factor a in breast cancer. DNA Cell Biol, 2014, 33(5):291-300.
13.	Yamauchi M, Nakayama Y, Minagawa N, et al. Mitochondrial transcription factor a worsens the clinical course of patients with pancreatic cancer through inhibition of apoptosis of cancer cells. Pancreas, 2014, 43(3):405-410.
14.	Jeng JY, Yeh TS, Lee JW, et al. Maintenance of mitochondrial DNA copy number and expression are essential for preservation of mitochondrial function and cell growth. J Cell Biochem, 2008, 103(2):347-357.
15.	曹宁川, 肖健. COX-2、VEGF及MMP-9蛋白在子宫内膜癌中的表达及意义.求医问药:学术版, 2012, 10(6):110-111.
16.	汪美华, 周林艳. COX-2, VEGF在结肠癌组织中的表达及其临床意义.肿瘤基础与临床, 2008, 21(4):294-295.
17.	王青, 赵华栋, 阴继凯, 等. COX-2和MMP-2在结肠癌组织中的表达及其临床意义.海南医学院学报, 2012, 18(5):614-616, 619.
18.	Samudio I, Fiegl M, Andreeff M. Mitochondrial uncoupling and the Warburg effect:molecular basis for the reprogramming of cancer cell metabolism. Cancer Res, 2009, 69(6):2163-2166.
19.	Kaufman BA, Durisic N, Mativetsky JM, et al. The mitochondrial transcription factor TFAM coordinates the assembly of multiple DNA molecules into nucleoid-like structures. Mol Biol Cell, 2007, 18(9):3225-3236.
20.	Ma PS, Clayton DA. Similarity of human mitochondrial transcription factor 1 to high mobility group proteins. Science, 1991, 252 (5008):965-969.
21.	Bao Y, Chen Z, Guo Y, et al. Tumor suppressor microRNA-27a in colorectal carcinogenesis and progression by targeting SGPP1 and Smad2. PLoS One, 2014, 9(8):e105991.
22.	Gopalan V, Smith RA, Lam AK. Downregulation of microRNA-498 in colorectal cancers and its cellular effects. Exp Cell Res, 2015, 330(2):423-428.
23.	Liu WD, Bo YZ, Fan Y, et al. Effect of Bcl-xL gene expression silenced by RNA interference on invasion of human colorectal cancer cells. J BUON, 2014, 19(4):925-929.
24.	You J, Fang N, Gu J, et al. Noncoding RNA small nucleolar RNA host gene 1 promote cell proliferation in nonsmall cell lung cancer. Indian J Cancer, 2014, 51 Suppl 3:e99-e102.
25.	Dimitrakopoulos GN, Dimitrakopoulou K, Maraziotis IA, et al. Supervised method for construction of microRNA-mRNA networks:Application in cardiac tissue aging dataset//Engineering in Medicine and Biology Society (EMBC). 2014 36th Annual International Conference of the IEEE, 2014:318-321.
26.	Long LM, He BF, Huang GQ, et al. microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2. Oncol Lett, 2015, 9(2):645-650.
27.	Li H, Gupta S, Du WW, et al. MicroRNA-17 inhibits tumor growth by stimulating T-cell mediated host immune response. Oncoscience, 2014, 1(7):531-539.
28.	彭伟, 杨韵, 刘韵霄, 等. microRNA在结直肠癌中的研究及应用进展.中国普外基础与临床杂志, 2013, 20(6):691-696.

1. Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide:sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer, 2015, 136(5):E359-E386.
2. Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin, 2011, 61(2):69-90.
3. Broussard EK, Disis ML. TNM staging in colorectal cancer:T is for T cell and M is for memory. J Clin Oncol, 2011, 29(6):601-603.
4. de la Cruz-Merino L, Henao Carrasco F, Vicente Baz D, et al. Immune microenvironment in colorectal cancer:a new hallmark to change old paradigms. Clin Dev Immunol, 2011, 2011:174149.
5. Lao VV, Grady WM. Epigenetics and colorectal cancer. Nat Rev Gastroenterol Hepatol, 2011, 8(12):686-700.
6. Mlecnik B, Tosolini M, Kirilovsky A, et al. Histopathologic-based prognostic factors of colorectal cancers are associated with the state of the local immune reaction. J Clin Oncol, 2011, 29(6):610-618.
7. Baffy G. Uncoupling protein-2 and cancer. Mitochondrion, 2010, 10(3):243-252.
8. Litonin D, Sologub M, Shi Y, et al. Human mitochondrial transcription revisited:only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. J Biol Chem, 2010, 285(24):18129-18133.
9. Mayevsky A. Mitochondrial function and energy metabolism in cancer cells:past overview and future perspectives. Mitochondrion, 2009, 9(3):165-179.
10. Toki N, Kagami S, Kurita T, et al. Expression of mitochondrial transcription factor A in endometrial carcinomas:clinicopathologic correlations and prognostic significance. Virchows Arch, 2010, 456(4):387-393.
11. Wen Z, Lei Z, Jin-An M, et al. The inhibitory role of miR-214 in cervical cancer cells through directly targeting mitochondrial transcription factor A (TFAM). Eur J Gynaecol Oncol, 2014, 35(6):676-682.
12. Yao J, Zhou E, Wang Y, et al. microRNA-200a inhibits cell proliferation by targeting mitochondrial transcription factor a in breast cancer. DNA Cell Biol, 2014, 33(5):291-300.
13. Yamauchi M, Nakayama Y, Minagawa N, et al. Mitochondrial transcription factor a worsens the clinical course of patients with pancreatic cancer through inhibition of apoptosis of cancer cells. Pancreas, 2014, 43(3):405-410.
14. Jeng JY, Yeh TS, Lee JW, et al. Maintenance of mitochondrial DNA copy number and expression are essential for preservation of mitochondrial function and cell growth. J Cell Biochem, 2008, 103(2):347-357.
15. 曹宁川, 肖健. COX-2、VEGF及MMP-9蛋白在子宫内膜癌中的表达及意义.求医问药:学术版, 2012, 10(6):110-111.
16. 汪美华, 周林艳. COX-2, VEGF在结肠癌组织中的表达及其临床意义.肿瘤基础与临床, 2008, 21(4):294-295.
17. 王青, 赵华栋, 阴继凯, 等. COX-2和MMP-2在结肠癌组织中的表达及其临床意义.海南医学院学报, 2012, 18(5):614-616, 619.
18. Samudio I, Fiegl M, Andreeff M. Mitochondrial uncoupling and the Warburg effect:molecular basis for the reprogramming of cancer cell metabolism. Cancer Res, 2009, 69(6):2163-2166.
19. Kaufman BA, Durisic N, Mativetsky JM, et al. The mitochondrial transcription factor TFAM coordinates the assembly of multiple DNA molecules into nucleoid-like structures. Mol Biol Cell, 2007, 18(9):3225-3236.
20. Ma PS, Clayton DA. Similarity of human mitochondrial transcription factor 1 to high mobility group proteins. Science, 1991, 252 (5008):965-969.
21. Bao Y, Chen Z, Guo Y, et al. Tumor suppressor microRNA-27a in colorectal carcinogenesis and progression by targeting SGPP1 and Smad2. PLoS One, 2014, 9(8):e105991.
22. Gopalan V, Smith RA, Lam AK. Downregulation of microRNA-498 in colorectal cancers and its cellular effects. Exp Cell Res, 2015, 330(2):423-428.
23. Liu WD, Bo YZ, Fan Y, et al. Effect of Bcl-xL gene expression silenced by RNA interference on invasion of human colorectal cancer cells. J BUON, 2014, 19(4):925-929.
24. You J, Fang N, Gu J, et al. Noncoding RNA small nucleolar RNA host gene 1 promote cell proliferation in nonsmall cell lung cancer. Indian J Cancer, 2014, 51 Suppl 3:e99-e102.
25. Dimitrakopoulos GN, Dimitrakopoulou K, Maraziotis IA, et al. Supervised method for construction of microRNA-mRNA networks:Application in cardiac tissue aging dataset//Engineering in Medicine and Biology Society (EMBC). 2014 36th Annual International Conference of the IEEE, 2014:318-321.
26. Long LM, He BF, Huang GQ, et al. microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2. Oncol Lett, 2015, 9(2):645-650.
27. Li H, Gupta S, Du WW, et al. MicroRNA-17 inhibits tumor growth by stimulating T-cell mediated host immune response. Oncoscience, 2014, 1(7):531-539.
28. 彭伟, 杨韵, 刘韵霄, 等. microRNA在结直肠癌中的研究及应用进展.中国普外基础与临床杂志, 2013, 20(6):691-696.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Mitochondrial Transcription Factor A in Colon Cancer and Its Role for Proliferative Regulation

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