Effect of Poria Cocos on Xenograft Tumor of Human Gastric Cancer SGC-7901 Cell Line in Nude Mice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIUYuan ^1,2 ,  QIANJun ¹ , GUOChen-xu ¹ , CHENGZe-nong ³

1. Department of The Third Tumor Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China;
2. Department of Graduate, Bengbu Medical College, Bengbu 233000, Anhui Province, China;
3. Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China;

Corresponding author：

QIANJun, Email: qianjun215036@sina.com

Keywords：

Poria cocos; Gastric cancer SGC-7901 cell line; Xenograft tumor in nude mice

DOI：

10.7507/1007-9424.20160079

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Objective To explore the effect of Poria cocos on xenograft tumors of gastric cancer SGC-7901 cell line in mude mice. Method ①After establishment of xenograft tumor of gastric cancer SGC-7901 cell line, 10 nude mice were equally divided into normal control group and Poria cocos group. The nude mice of each group were gavaged with normal saline (NS) and Poria cocos (0.5 mL) for 32 days, respectively. Tumor volume were measured to draw tumor growth curves and the tumor weight inhibitory rate was calculated with tumor weight (on the 32-day, nude mice were sacrificed to get the xenograft tumors). The expressions of B cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax), Caspase-3, Caspase-9, and vascular endothelial growth factor (VEGF) were detected by immunohistochemical staining. ②Preparation of drug serum containing Poria cocos. Gastric cancer SGC-7901 cell line were be divided into 2 groups: normal control group and Poria cocos group. Cells of normal control group were treated with serum containing NS, and cells of Poria cocos group were treated with drug serum containing 10% Poria cocos. After 24 hours and 48 hours, Western-blot was used to detect the expressions of Bcl-2 and Bax. Results On 32-day, the volume and weight of xenograft tumors in normal control group〔(2 652.17±225.01) mm3 and (2.48±0.21) g〕were both higher than those of Poria cocos group〔(1 247.56±277.23) mm3 and (1.28±0.28) g〕, P<0.050. The tumor inhibitory rate in Poria cocos group was 48.39%. The results of immunohistochemical staining showed that, compared with normal control group, Poria cocos could down-regulate the expressions of Bcl-2〔(4.20±1.10）score vs. (8.00±1.20) score〕and VEGF〔(3.80±0.45) score vs. (7.80±1.10) score〕, while up-regulate the expressions of Bax〔(7.40±1.34) score vs. (3.00±0.71) score〕, Caspase-3〔(6.60±1.34) score vs. (2.60±0.55) score〕, and Caspase-9〔(7.20±1.79) score vs. (4.00±1.22) score〕, P<0.050. Compared with normal control group (1.72±0.03), the expression value of Bcl-2 was all higher in 24 h-Poria cocos group (0.96±0.04) and 48 h-Poria cocos group (0.77±0.04), P<0.050, and the expression value was higher in 48 h-Poria cocos group than that of 24 h-Poria cocos group (P<0.050). Compared with normal control group (0.15±0.01), the expression value of Bax was higher in 48 h-Poria cocos group (0.55±0.01), P<0.050, but there was no significant difference between the normal control group and 24 h-Poria cocos group（0.19±0）, P>0.050. Conclusions Poria cocos can restrain the growth of xenograft tumors for gastric cancer SGC-7901 cell line in mude mice, and the mechanism may be related to mitochondrial apoptosis pathway and the inhibition of expression of VEGF.

Citation： LIUYuan, QIANJun, GUOChen-xu, CHENGZe-nong. Effect of Poria Cocos on Xenograft Tumor of Human Gastric Cancer SGC-7901 Cell Line in Nude Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(3): 286-290. doi: 10.7507/1007-9424.20160079 Copy

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3.	Shan H, Qinglin Z, Fengjun X, et al. Reversal of multidrug resistance of KBV200 cells by triterpenoids isolated from Poria cocos. Planta Med, 2012, 78(5): 428-433.
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12.	Chawla-Sarkar M, Leaman DW, Borden EC. Preferential induction of apoptosis by interferon (IFN)-beta compared with IFN-alpha2: correlation with TRAIL/Apo2L induction in melanoma cell lines. Clin Cancer Res, 2001, 7(6): 1821-1831.
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17.	Wood WG, Igbavboa U, Muller WE, et al. Statins, Bcl-2, and apop-tosis: cell death or cell protection?. Mol Neurobiol, 2013, 48(2): 308-314.
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19.	Wuillème-Toumi S, Robillard N, Gomez P, et al. Mcl-1 is overexp-ressed in multiple myeloma and associated with relapse and shorter survival. Leukemia, 2005, 19(7): 1248-1252.
20.	Devarajan E, Sahin AA, Chen JS, et al. Down-regulation of caspase 3 in breast cancer: a possible mechanism for chemoresistance. Oncogene, 2002, 21(57): 8843-8851.
21.	Fong PY, Xue WC, Ngan HY, et al. Caspase activity is downregu-lated in choriocarcinoma: a cDNA array differential expression study. J Clin Pathol, 2006, 59(2): 179-183.
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23.	Du K, Gong HY, Gong ZM. Influence of serum VEGF levels on therapeutic outcome and diagnosis/prognostic value in patients with cervical cancer. Asian Pac J Cancer Prev, 2014, 15(20): 8793-8796.
24.	Chambers SK, Clouser MC, Baker AF, et al. Overexpression oftumor vascular endothelial growth factor A may portend an incre-ased likelihood of progression in a phase Ⅱ trial of bevacizumab anderlotinib in resistant ovarian cancer. Clin Cancer Res, 2010, 16(21): 5320-5328.
25.	Siddiqui GK, Elmasry K, Wong Te Fong AC, et al. Prognostic significance of intratumoral vascular endothelial growth factor as a marker of tumour angiogenesis in epithelial ovarian cancer. Eur J Gynaecol Oncol, 2010, 31(2): 156-159.

1. Goh LY, Leow AH, Goh KL. Observations on the epidemiology of gastrointestinal and liver cancers in the Asia-Pacific region. J Dig Dis, 2014, 15(9): 463-468.
2. Zhang M, Chiu LC, Cheung PC, et al. Growth-inhibitory effects of a beta-glucan from the mycelium of Poria cocos on human breast carcinoma MCF-7 cells: cell-cycle arrest and apoptosis induction. Oncol Rep, 2006, 15(3): 637-643.
3. Shan H, Qinglin Z, Fengjun X, et al. Reversal of multidrug resistance of KBV200 cells by triterpenoids isolated from Poria cocos. Planta Med, 2012, 78(5): 428-433.
4. Cheng S, Swanson K, Eliaz I, et al. Pachymic acid inhibits growth and induces apoptosis of pancreatic cancer in vitro and in vivo by targeting ER stress. PLoS One, 2015, 10(4): e0122270.
5. 王婷, 张静霞, 程海祺, 等. 高效液相色谱法测定利福拉齐的有关物质. 中国抗生素杂志, 2012, 37(4): 284-287.
6. 许良中, 杨文涛. 免疫组织化学反应结果的判断标准. 中国癌症杂志, 1996, 6(4): 229-231.
7. Yang ND, Tan SH, Ng S, et al. Artesunate induces cell death in human cancer cells via enhancing lysosomal function and lysosomal degradation of ferritin. J Biol Chem, 2014, 289(48): 33425-33441.
8. Hao W, Wang S, Zhou Z. Tubeimoside-1 (TBMS1) inhibits lung cancer cell growth and induces cells apoptosis through activation of MAPK-JNK pathway. Int J Clin Exp Pathol, 2015, 8(10): 12075-12083.
9. 张卫元, 杨阳, 陈翠兰, 等. 茯苓散对免疫低下小鼠免疫功能的影响. 中国兽医学报, 2014, 34(2): 283-285.
10. Li H, Li X, Bai M, et al. Matrine inhibited proliferation and increased apoptosis in human breast cancer MCF-7 cells via upregulation of Bax and downregulation of Bcl-2. Int J Clin Exp Pathol, 2015, 8(11): 14793-14799.
11. Giacoppo S, Soundara Rajan T, Galuppo M, et al. Purified cannabi-diol, the main non-psychotropic component of cannabis sativa, alone, counteracts neuronal apoptosis in experimental multiple sclerosis. Eur Rev Med Pharmacol Sci, 2015, 19(24): 4906-4919.
12. Chawla-Sarkar M, Leaman DW, Borden EC. Preferential induction of apoptosis by interferon (IFN)-beta compared with IFN-alpha2: correlation with TRAIL/Apo2L induction in melanoma cell lines. Clin Cancer Res, 2001, 7(6): 1821-1831.
13. Yuan S, Akey CW. Apoptosome structure, assembly, and procaspase activation. Structure, 2013, 21(4): 501-515.
14. Boulares AH, Zoltoski AJ, Yakovlev A, et al. Roles of DNA fragmen-tation factor and poly(ADP-ribose) polymerase in an amplification phase of tumor necrosis factor-induced apoptosis. J Biol Chem, 2001, 276(41): 38185-38192.
15. Tischner D, Woess C, Ottina E, et al. Bcl-2-regulated cell death signalling in the prevention of autoimmunity. Cell Death Dis, 2010, 1: e48.
16. Zhang Y, Bao YL, Wu Y, et al. Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway. Mol Med Rep, 2013, 8(4): 967-972.
17. Wood WG, Igbavboa U, Muller WE, et al. Statins, Bcl-2, and apop-tosis: cell death or cell protection?. Mol Neurobiol, 2013, 48(2): 308-314.
18. Pietrantonio F, Biondani P, De Braud F, et al. Bax expression is predictive of favorable clinical outcome in chemonaive advanced gastric cancer patients treated with capecitabine, oxaliplatin, and irinotecan regimen. Transl Oncol, 2012, 5(3): 155-159.
19. Wuillème-Toumi S, Robillard N, Gomez P, et al. Mcl-1 is overexp-ressed in multiple myeloma and associated with relapse and shorter survival. Leukemia, 2005, 19(7): 1248-1252.
20. Devarajan E, Sahin AA, Chen JS, et al. Down-regulation of caspase 3 in breast cancer: a possible mechanism for chemoresistance. Oncogene, 2002, 21(57): 8843-8851.
21. Fong PY, Xue WC, Ngan HY, et al. Caspase activity is downregu-lated in choriocarcinoma: a cDNA array differential expression study. J Clin Pathol, 2006, 59(2): 179-183.
22. Dobrzycka B, Terlikowski SJ, Kowalczuk O, et al. Serum levels of VEGF and VEGF-C in patients with endometrial cancer. Eur Cytokine Netw, 2011, 22(1): 45-51.
23. Du K, Gong HY, Gong ZM. Influence of serum VEGF levels on therapeutic outcome and diagnosis/prognostic value in patients with cervical cancer. Asian Pac J Cancer Prev, 2014, 15(20): 8793-8796.
24. Chambers SK, Clouser MC, Baker AF, et al. Overexpression oftumor vascular endothelial growth factor A may portend an incre-ased likelihood of progression in a phase Ⅱ trial of bevacizumab anderlotinib in resistant ovarian cancer. Clin Cancer Res, 2010, 16(21): 5320-5328.
25. Siddiqui GK, Elmasry K, Wong Te Fong AC, et al. Prognostic significance of intratumoral vascular endothelial growth factor as a marker of tumour angiogenesis in epithelial ovarian cancer. Eur J Gynaecol Oncol, 2010, 31(2): 156-159.

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Effect of Poria Cocos on Xenograft Tumor of Human Gastric Cancer SGC-7901 Cell Line in Nude Mice

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