Effect of Jejunoileal Bypass on Protein Tyrosine Phosphatase 1B Protein Expression in Skeletal Muscle of Rats with Type 2 Diabetes Mellitus_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HEJin-song ¹ , ZHANGJing-xiao ² , LILi-fa ¹ ,  WEIShou-jiang ¹

1. Department of Gastrointestinal Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;
2. Department of Cardiology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;

Corresponding author：

WEIShou-jiang, Email: sichuanweihoujiang@126.com

Keywords：

Type 2 diabetes mellitus; Jejunoileal bypass; Protein tyrosine phosphatase 1B; Insulin resistance

DOI：

10.7507/1007-9424.20160240

Video：

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Objective To investigate role and mechanism of protein tyrosine phosphatase 1B (PTP1B) in jejunoileal bypass to treating rats with type 2 diabetes mellitus (T2DM). Methods Twenty-four T2DM SD rats and 24 normal SD rats were selected randomly by using random number table, then the SD rats with T2DM were randomly divided into jejunoileal bypass operation (DJBO, n=12) group and sham operation (DSO, n=12) group, the SD rats with normal food diet were randomly divided into jejunoileal bypass operation (NJBO, n=12) group and sham operation (NSO, n=12) group. Subsequently, fasting body weight (FBW), fasting plasma glucose (FPG), fasting insulin (FINS), and homeostasis model-insulin resistant (HOMA-IR) index of rats in each group were tested at different time points (before operation, on week 4 and 8 after operation). In addition, expression of PTP1B protein in skeletal muscle was determined by immunohistochemical staining and Western blot method respectively. Results ① The FBW before making T2DM model had no significant difference between the rats with high-fat diet and with normal diet (P > 0.05), which on week 4 or 8 after making T2DM model in the rats with high-fat diet was significantly heavier than that in the rats with normal diet (P < 0.05). ② Before jejunoileal bypass operation, the FBW, FPG, FINS, and HOMA-IR index in the DJBO group and the DSO group were significantly higher than those in the NJBO group and the NSO group (P < 0.05), respectively, which had no significant differences between the DJBO group and the DSO group (P > 0.05) and between the NJBO group and the NSO group (P > 0.05). ③ Compared with the values before jejunoileal bypass operation, the FBW, FPG, FINS, and HOMA-IR index on week 4 or 8 after jejunoileal bypass operation were significantly decreased in the DJBO group (P < 0.05); the FBW was significantly increased on week 4 or 8 after jejunoileal bypass operation in the DSO group and the NSO group (P < 0.05), and on week 8 after jejunoileal bypass operation in the NJBO group (P < 0.05). The other indexes had no significant differences between before and after jejunoileal bypass operation in the DSO group, the NSO group, or the NJBO group (P > 0.05). ④ On week 8 after jejunoileal bypass operation, the expression of PTP1B protein in the DSO group was significantly higher than that in the DJBO group, the NSO group or the NJBO group (P < 0.05), which in the DJBO group was significantly higher than that in the NSO group (P < 0.05) or the NJBO group (P < 0.05), which had no significant difference between the NJBO group and the NSO group (P > 0.05). Conclusion Jejunoileal bypass could effectively improve insulin resistance and decrease FPG level and FBW of T2DM rats through inhibiting expression of PTP1B protein in skeletal muscle of rat with T2DM.

Citation： HEJin-song, ZHANGJing-xiao, LILi-fa, WEIShou-jiang. Effect of Jejunoileal Bypass on Protein Tyrosine Phosphatase 1B Protein Expression in Skeletal Muscle of Rats with Type 2 Diabetes Mellitus. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(8): 910-914. doi: 10.7507/1007-9424.20160240 Copy

1.	www. who. int/topics/diabetes..
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6.	Cummings DE, Overduin J, Foster-Schubert KE. Gastric bypass for obesity:mechanisms of weight loss and diabetes resolution. J Clin Endocrinol Metab, 2004, 89(6):2608-2615.
7.	Handisurya A, Riedl M, Vila G, et al. Serum vaspin concentrations in relation to insulin sensitivity following RYGB-induced weight loss. Obes Surg, 2010, 20(2):198-203.
8.	Bonhomme S, Guijarro A, Keslacy S, et al. Gastric bypass up-regulates insulin signaling pathway. Nutrition, 2011, 27(1):73-80.
9.	Scapin G, Patel SB, Becker, et al. The structural basis for the selectivity of benzotriazole inhibitors of PTP1B. Biochemistry, 2003, 42(39):11451-11459.
10.	Haj FG, Verveer PJ, Squire A, et al. Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum. Science, 2002, 295(2260):1708-1711.
11.	Elchebly M, Payette P, Michaliszyn E, et al. Increased insulin sensitivity and obesity resistant in mice lacking the protein tyrosine phosphatase-1B gene. Science, 1999, 283(5407):1544-1548.
12.	Cho SY, Baek JY, Ham SS, et al. PTP1B inhibitors:Cyclopenta[d] [1, 2]-oxazine derivatives. Bioorg Med Chem Lett, 2006, 16(3):499-502.
13.	Zabolotny JM, Haj FG, Kim YB, et al. Transgenic overexpression of protein-tyrosine phosphatase 1B in muscle cause insulin resistance, but overexpression with leckocyte antigen-related phosphatase does not additively impair insulin action. J Biol Chem, 2004, 279(23):24844-24851.
14.	Taghibiglou C, Rashid-Kolvear F, Van Iderstine SC, et al. Hepatic very low density lipoprotein-ApoB overproduction is associated with attenuated hepatic insulin signaling and overexpression of protein-tyrosine phosphatase 1B in a fructose-fed hamster model of insulin resistance. J Biol Chem, 2002, 277(1):793-803.
15.	Araujo AC, Bonfleur ML, Balbo SL, et al. Duodenal-jejunal bypass surgery enhances glucose tolerance and beta-cell function in Western diet obese rats. Obes Surg, 2012, 22(5):819-826.
16.	Peterli R, Wölnerhanssen B, Peters T, et al. Improvement in glucose metabolism after baricatric surgery:comparison of lapaoscopic Roux-en-Y gastric bypass and laparosopic sleeve gastrectomy. Ann Surg, 2009, 250(2):234-241.]Sørensen SM, Savran MM, Konge L, et al. Three-dimensional versus two-dimensional vision in laparoscopy:a systematic review. Surg Endosc, 2016, 30(1):11-23.

1. www. who. int/topics/diabetes..
2. Pories WJ, Swanson MS, MacDonald KG, et al. Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg, 1995, 222(3):339-350.
3. Buchwald H, Avidor Y, Braunwald E, et al. Bariatric surgery:a systematic review and meta-analysis. JAMA, 2004, 292(14):1724-1737.
4. Vetter ML, Cardillo S, Rickels MR, et al. Narrative review:effect of bariatric surgery on type 2 diabetes mellitus. Ann Intern Med, 2009, 150(2):94-103.
5. Rubino F, Marescaux J. Effect of duodenal-jejunal exclusion in a non-obese animal model of type 2 diabetes:a new perspective for an old disease. Ann Surg, 2004, 239(1):1-11.
6. Cummings DE, Overduin J, Foster-Schubert KE. Gastric bypass for obesity:mechanisms of weight loss and diabetes resolution. J Clin Endocrinol Metab, 2004, 89(6):2608-2615.
7. Handisurya A, Riedl M, Vila G, et al. Serum vaspin concentrations in relation to insulin sensitivity following RYGB-induced weight loss. Obes Surg, 2010, 20(2):198-203.
8. Bonhomme S, Guijarro A, Keslacy S, et al. Gastric bypass up-regulates insulin signaling pathway. Nutrition, 2011, 27(1):73-80.
9. Scapin G, Patel SB, Becker, et al. The structural basis for the selectivity of benzotriazole inhibitors of PTP1B. Biochemistry, 2003, 42(39):11451-11459.
10. Haj FG, Verveer PJ, Squire A, et al. Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum. Science, 2002, 295(2260):1708-1711.
11. Elchebly M, Payette P, Michaliszyn E, et al. Increased insulin sensitivity and obesity resistant in mice lacking the protein tyrosine phosphatase-1B gene. Science, 1999, 283(5407):1544-1548.
12. Cho SY, Baek JY, Ham SS, et al. PTP1B inhibitors:Cyclopenta[d] [1, 2]-oxazine derivatives. Bioorg Med Chem Lett, 2006, 16(3):499-502.
13. Zabolotny JM, Haj FG, Kim YB, et al. Transgenic overexpression of protein-tyrosine phosphatase 1B in muscle cause insulin resistance, but overexpression with leckocyte antigen-related phosphatase does not additively impair insulin action. J Biol Chem, 2004, 279(23):24844-24851.
14. Taghibiglou C, Rashid-Kolvear F, Van Iderstine SC, et al. Hepatic very low density lipoprotein-ApoB overproduction is associated with attenuated hepatic insulin signaling and overexpression of protein-tyrosine phosphatase 1B in a fructose-fed hamster model of insulin resistance. J Biol Chem, 2002, 277(1):793-803.
15. Araujo AC, Bonfleur ML, Balbo SL, et al. Duodenal-jejunal bypass surgery enhances glucose tolerance and beta-cell function in Western diet obese rats. Obes Surg, 2012, 22(5):819-826.
16. Peterli R, Wölnerhanssen B, Peters T, et al. Improvement in glucose metabolism after baricatric surgery:comparison of lapaoscopic Roux-en-Y gastric bypass and laparosopic sleeve gastrectomy. Ann Surg, 2009, 250(2):234-241.]Sørensen SM, Savran MM, Konge L, et al. Three-dimensional versus two-dimensional vision in laparoscopy:a systematic review. Surg Endosc, 2016, 30(1):11-23.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Jejunoileal Bypass on Protein Tyrosine Phosphatase 1B Protein Expression in Skeletal Muscle of Rats with Type 2 Diabetes Mellitus

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