The Role of Tumor Necrosis Factor-α in Pancreatitis Associated Adrenal Cells' Apoptosis of Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YANGXiao-jia , SHIQiao , YUJia , CHENChen , HEBin , ZHAOLiang , WANGPeng , HUPeng , LIChen , GUOWen-yi ,  WANGWei-xing

Department of Hepatobiliary Endoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China;

Corresponding author：

WANGWei-xing, Email: sate.llite@163.com

Keywords：

Severe acute pancreatitis; Adrenal injury; Tumor necrosis factor-α

DOI：

10.7507/1007-9424.20160307

Video：

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Objective To investigate The role of tumor necrosis factor-α (TNF-α) in pancreatitis-associated adrenal cells' apoptosis of severe acute pancreatitis (SAP). Methods Forty Wistar rats were randomly divided into sham operation group (SO group) and SAP group by random number method, the SAP group was divided into 3, 6, 12, and 24 h 4 subgroups, 8 rats in each group. SAP model was induced by retrograde injection of 5% sodium taurocholate into the bilipancreatic duct. At 3, 6, 12, and 24 h after operation, serum amylase and lipase was measured, adrenal injury was evaluated by histological examination, apoptosis of the adrenal cells was observed by TUNEL method, and expressions of TNF-α and Caspase-3 protein were detected by Western blot. Results The levels of serum AMY and LIP, histopathological scores of pancreatic tissues and adrenal tissues at each time point after operation in SAP group increased significantly than SO group (P < 0.05). With the duration extension of SAP, the apoptosis index of adrenal cells in SAP group progressively heightened, and were higher than those in the SO group (P < 0.05). And the expressions of TNF-α and Caspase-3 protein in adrenal tissues of SAP group gradually increased, at 24 h this data slightly decreased, but still higher than SO group (P < 0.05). Conclusion TNF-α may be involved in the pathogenesis of adrenal injury in SAP rats by activate the protein expression of Caspase-3.

Citation： YANG Xiao-jia, SHI Qiao, YU Jia, CHEN Chen, HE Bin, ZHAO Liang, WANG Peng, HU Peng, LI Chen, GUO Wen-yi, WANG Wei-xing. The Role of Tumor Necrosis Factor-α in Pancreatitis Associated Adrenal Cells' Apoptosis of Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(10): 1198-1203. doi: 10.7507/1007-9424.20160307 Copy

1.	杨波,郭闻一,余佳,等. PARP及NF-κB在重症急性胰腺炎大鼠肾上腺细胞中的表达及5-AIQ/PDTC的干预作用. 中华医学杂志, 2013, 93(38):3063-3067.
2.	Lei H, Minghao W, Xiaonan Y, et al. Acute lung injury in patients with severe acute pancreatitis. Turk J Gastroenterol, 2013, 24(6):502-507.
3.	Akbarshahi H, Rosendahl AH, Westergren-Thorsson G, et al. Acute lung injury in acute pancreatitis-awaiting the big leap. Respir Med, 2012, 106(9):1199-1210.
4.	Yu J, Deng W, Wang W, et al. Inhibition of poly (ADP-ribose) polymerase attenuates acute kidney injury in sodium taurocholate-induced acute pancreatitis in rats. Pancreas, 2012, 41(8):1299-1305.
5.	De Waele JJ, Hoste EA, Baert D, et al. Relative adrenal insufficiency in patients with severe acute pancreatitis. Intensive Care Med, 2007, 33(10):1754-1760.
6.	Schmidt J, Rattner DW, Lewandrowski K, et al. A better model of acute pancreatitis for evaluating therapy. Ann Surg, 1992, 215(1):44-56.
7.	Deng W, Hui Y, Yu J, et al. A new pathological scoring method for adrenal injury in rats with severe acute pancreatitis. Pathol Res Pract, 2014, 210(12):1011-1017.
8.	Lopez-Font I, Gea-Sorli S, De-Madaria E, et al. Pancreatic and pulmonary mast cells activation during experimental acute pancreatitis. World J Gastroenterol, 2010, 16(27):3411-3417.
9.	周华,许媛. 重症患者的肾上腺皮质功能不全. 医学新知杂志, 2007, 17(5):258-260.
10.	Bozzo A, Sonez C A, Mugnaini M T, et al. Chronic stress effects on the apoptotic index of the adrenal cortex of pregnant rats. Biocell, 2006, 30(3):439-445.
11.	Bollen TL, van Santvoort HC, Besselink MG, et al. Intense adrenal enhancement in patients with acute pancreatitis and early organ failure. Emerg Radiol, 2007, 14(5):317-322.
12.	Lesniewska B, Nowak KW, Malendowicz LK. Dexamethasone-induced adrenal cortex atrophy and recovery of the gland from partial, steroid-induced atrophy. Exp Clin Endocrinol, 1992, 100(3):133-139.
13.	黄建华, 沈自尹,刘小雨,等. 淫羊藿总黄酮拮抗皮质酮大鼠肾上腺皮质细胞凋亡的研究. 中医药学刊, 2006, 24(9):1664-1666.
14.	余佳,王卫星,徐胜,等. 急性胰腺炎大鼠肾上腺损伤中bax与bcl-2表达的研究. 中华实验外科杂志, 2011, 28(10):1726-1728.
15.	Wang Y, Singh R, Lefkowitch JH, et al. Tumor necrosis factor-induced toxic liver injury results from JNK2-dependent activation of caspase-8 and the mitochondrial death pathway. J Biol Chem, 2006, 281(22):15258-15267.
16.	Li S, Zhao Y, He X, et al. Relief of extrinsic pathway inhibition by the Bid-dependent mitochondrial release of Smac in Fas-mediated hepatocyte apoptosis. J Biol Chem, 2002, 277(30):26912-26920.
17.	Jiang X, Wang X. Cytochrome c promotes caspase-9 activation by inducing nucleotide binding to Apaf-1. J Biol Chem, 2000, 275(40):31199-31203.
18.	Du C, Fang M, Li Y, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell, 2000, 102(1):33-42.
19.	Romano G, Briguori C, Quintavalle C, et al. Contrast agents and renal cell apoptosis. Eur Heart J, 2008, 29(20):2569-2576.
20.	Hayashi M, Araki T. Caspase in renal development. Nephrol Dial Transplant, 2002, 17 Suppl 9:8-10.
21.	Fan G, Ma X, Wong PY, et al. p53 dephosphorylation and p21(Cip1/Waf1) translocation correlate with caspase-3 activation in TGF-beta1-induced apoptosis of HuH-7 cells. Apoptosis, 2004, 9(2):211-221.
22.	臧国庆,俞红,周霞秋,等. TNF-α体外介导小鼠肝细胞凋亡和坏死. 世界华人消化杂志, 2000, 8(3):303-306.
23.	Leist M, Gantner F, Bohlinger I, et al. Tumor necrosis factor-induced hepatocyte apoptosis precedes liver failure in experimental murine shock models. Am J Pathol, 1995, 146(5):1220-1234.

1. 杨波,郭闻一,余佳,等. PARP及NF-κB在重症急性胰腺炎大鼠肾上腺细胞中的表达及5-AIQ/PDTC的干预作用. 中华医学杂志, 2013, 93(38):3063-3067.
2. Lei H, Minghao W, Xiaonan Y, et al. Acute lung injury in patients with severe acute pancreatitis. Turk J Gastroenterol, 2013, 24(6):502-507.
3. Akbarshahi H, Rosendahl AH, Westergren-Thorsson G, et al. Acute lung injury in acute pancreatitis-awaiting the big leap. Respir Med, 2012, 106(9):1199-1210.
4. Yu J, Deng W, Wang W, et al. Inhibition of poly (ADP-ribose) polymerase attenuates acute kidney injury in sodium taurocholate-induced acute pancreatitis in rats. Pancreas, 2012, 41(8):1299-1305.
5. De Waele JJ, Hoste EA, Baert D, et al. Relative adrenal insufficiency in patients with severe acute pancreatitis. Intensive Care Med, 2007, 33(10):1754-1760.
6. Schmidt J, Rattner DW, Lewandrowski K, et al. A better model of acute pancreatitis for evaluating therapy. Ann Surg, 1992, 215(1):44-56.
7. Deng W, Hui Y, Yu J, et al. A new pathological scoring method for adrenal injury in rats with severe acute pancreatitis. Pathol Res Pract, 2014, 210(12):1011-1017.
8. Lopez-Font I, Gea-Sorli S, De-Madaria E, et al. Pancreatic and pulmonary mast cells activation during experimental acute pancreatitis. World J Gastroenterol, 2010, 16(27):3411-3417.
9. 周华,许媛. 重症患者的肾上腺皮质功能不全. 医学新知杂志, 2007, 17(5):258-260.
10. Bozzo A, Sonez C A, Mugnaini M T, et al. Chronic stress effects on the apoptotic index of the adrenal cortex of pregnant rats. Biocell, 2006, 30(3):439-445.
11. Bollen TL, van Santvoort HC, Besselink MG, et al. Intense adrenal enhancement in patients with acute pancreatitis and early organ failure. Emerg Radiol, 2007, 14(5):317-322.
12. Lesniewska B, Nowak KW, Malendowicz LK. Dexamethasone-induced adrenal cortex atrophy and recovery of the gland from partial, steroid-induced atrophy. Exp Clin Endocrinol, 1992, 100(3):133-139.
13. 黄建华, 沈自尹,刘小雨,等. 淫羊藿总黄酮拮抗皮质酮大鼠肾上腺皮质细胞凋亡的研究. 中医药学刊, 2006, 24(9):1664-1666.
14. 余佳,王卫星,徐胜,等. 急性胰腺炎大鼠肾上腺损伤中bax与bcl-2表达的研究. 中华实验外科杂志, 2011, 28(10):1726-1728.
15. Wang Y, Singh R, Lefkowitch JH, et al. Tumor necrosis factor-induced toxic liver injury results from JNK2-dependent activation of caspase-8 and the mitochondrial death pathway. J Biol Chem, 2006, 281(22):15258-15267.
16. Li S, Zhao Y, He X, et al. Relief of extrinsic pathway inhibition by the Bid-dependent mitochondrial release of Smac in Fas-mediated hepatocyte apoptosis. J Biol Chem, 2002, 277(30):26912-26920.
17. Jiang X, Wang X. Cytochrome c promotes caspase-9 activation by inducing nucleotide binding to Apaf-1. J Biol Chem, 2000, 275(40):31199-31203.
18. Du C, Fang M, Li Y, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell, 2000, 102(1):33-42.
19. Romano G, Briguori C, Quintavalle C, et al. Contrast agents and renal cell apoptosis. Eur Heart J, 2008, 29(20):2569-2576.
20. Hayashi M, Araki T. Caspase in renal development. Nephrol Dial Transplant, 2002, 17 Suppl 9:8-10.
21. Fan G, Ma X, Wong PY, et al. p53 dephosphorylation and p21(Cip1/Waf1) translocation correlate with caspase-3 activation in TGF-beta1-induced apoptosis of HuH-7 cells. Apoptosis, 2004, 9(2):211-221.
22. 臧国庆,俞红,周霞秋,等. TNF-α体外介导小鼠肝细胞凋亡和坏死. 世界华人消化杂志, 2000, 8(3):303-306.
23. Leist M, Gantner F, Bohlinger I, et al. Tumor necrosis factor-induced hepatocyte apoptosis precedes liver failure in experimental murine shock models. Am J Pathol, 1995, 146(5):1220-1234.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Role of Tumor Necrosis Factor-α in Pancreatitis Associated Adrenal Cells' Apoptosis of Severe Acute Pancreatitis

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