Effect of different resuscitation fluids on interleukin-8 and tumor necrosis factor-α in rats with traumatic hemorrhagic shock_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Xuexin , LIU Jian ,  ZHANG Dewei

The Third Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110001, P.R.China;

Corresponding author：

ZHANG Dewei, Email: syzhangdewei@sohu.com

Keywords：

traumatic hemorrhagic shock; limited fluid resuscitation; tumor necrosis factor-α; interleukin-8; rat

DOI：

10.7507/1007-9424.201606020

Video：

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Objective To investigate effect of different resuscitation liquids and different resuscitation methods on contents of interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in early resuscitation process of rats with traumatic hemorrhagic shock. Methods Sixty-four healthy SD rats (450–550 g) were chosen and divided into 4 groups randomly and averagely: crystal liquid limited resuscitation group, colloidal liquid limited resuscitation group, 7.5% NaCl limited resuscitation group, and colloidal liquid non-limited resuscitation group. There were 16 rats in each group. All the experimental rats were weighed before intraperitoneal injection of pentobarbital sodium anesthesia. Animal model was established via Chaudry’s method. The rats were killed and the abdominal aorta bloods were drew on hour 2, 6, 12, and 24 after recovering from anesthesia. The contents of IL-8 and TNF-α in plasmas were detected by enzyme linked immunosorbent assay. Results The contents of IL-8 and TNF-α among three kinds of limited resuscitation groups on hour 6 after resuscitation were significantly higher than those on hour 2 after resuscitation (P<0.05) and reached the peaks, then began to decrease. On hour 12 after resuscitation, the contents of IL-8 and TNF-α were decreased continuously among three kinds of limited resuscitation groups (P<0.05). The contents of IL-8 and TNF-α in the colloidal liquid non-limited resuscitation group at each point time were significantly higher than those among three kinds of limited resuscitation groups (P<0.05), which in the crystal liquid resuscitation group were significantly lower than those in the other limited liquid resuscitation groups (P<0.05). Conclusions In process of liquid resuscitation of rats with traumatic hemorrhagic shock, limited resuscitation method is better than that of non-limited resuscitation method. Among three kinds of limited resuscitation methods, crystal resuscitation liquid is more effective than the other two resuscitation liquids in prohibiting releases of IL-8 and TNF-α in rats with traumatic hemorrhagic shock.

Citation： LIXuexin, LIUJian, ZHANGDewei. Effect of different resuscitation fluids on interleukin-8 and tumor necrosis factor-α in rats with traumatic hemorrhagic shock. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2017, 24(2): 174-178. doi: 10.7507/1007-9424.201606020 Copy

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11.	Coletta C, Módis K, Oláh G,et al. Endothelial dysfunction is a potential contributor to multiple organ failure and mortality in aged mice subjected to septic shock: preclinical studies in a murine model of cecal ligation and puncture. Crit Care, 2014, 18(5): 511.
12.	Binkowska AM, Michalak G, Słotwiński R. Current views on the mechanisms of immune responses to trauma and infection. Cent Eur J Immunol, 2015, 40(2): 206-216.
13.	Jackman P. Immunomodulation in transfused trauma patients. Curr Opin Anaesthesiol, 2013, 26(2): 196-203.
14.	Murdock AD, Berséus O, Hervig T,et al. Whole blood: the future of traumatic hemorrhagic shock resuscitation. Shock, 2014, 41(Suppl 1): 62-69.
15.	Du Z, Jia H, Liu J,et al. Effects of three hydrogen-rich liquids on hemorrhagic shock in rats. J Surg Res, 2015, 193(1): 377-382.
16.	Holcomb JB, Pati S. Optimal trauma resuscitation with plasma as the primary resuscitative fluid: the surgeon’s perspective. Hematology Am Soc Hematol Educ Program, 2013, 2013: 656-659.
17.	Siwetz M, Blaschitz A, El-Heliebi A,et al. TNF-α alters the inflammatory secretion profile of human first trimester placenta. Lab Invest, 2016, 96(4): 428-438.
18.	董彪, 关维雨, 张德巍. 创伤失血性休克限制性液体复苏的治疗进展. 中国现代普通外科进展, 2014, 17(7): 576-579.
19.	Kampmeier T, Rehberg S, Ertmer C,et al. Evolution of fluid therapy. Best Pract Res Clin Anaesthesiol, 2014, 28(3): 207-216.
20.	Stancil A. Past and current trends in intravenous therapy for hemorrhagic shock. Air Med J, 2014, 33(4): 172-175.
21.	Spahn DR, Bouillon B, Cerny V,et al. Management of bleeding and coagulopathy following major trauma: an updated European guideline. Crit Care, 2013, 17(2): R76.
22.	Petroni RC, Biselli PJ, Lima TM,et al. Impact of time on fluid resuscitation with hypertonic saline (NaCl 7.5%) in rats with LPS-induced acute lung injury. Shock, 2015, 44(6): 609-615.
23.	Godinho M, Padim P, Evora PR,et al. Curbing inflammation in hemorrhagic trauma: a review. Rev Col Bras Cir, 2015, 42(4): 273-278.
24.	Delano J, Rizoli B, Rhind G,et al. Prehospital resuscitation of traumatic hemorrhagic shock with hypertonic solutions worsens hypocoagulation and hyperfibrinolysis. Shock, 2015, 44(1): 25-31.
25.	Sordi R, Chiazza F, Johnson FL,et al. Inhibition of IκB kinase attenuates the organ injury and dysfunction associated with hemorrhagic shock. Mol Med, 2015, 21(21): 563-575.
26.	Jeong KY, Suh GJ, Kwon WY,et al. The therapeutic effect and mechanism of niacin on acute lung injury in a rat model of hemorrhagic shock: Down-regulation of the reactive oxygen species-dependent nuclear factor κB pathway. J Trauma Acute Care Surg, 2015, 79(2): 247-255.

1. 赵晓东. 战(创)伤失血性休克的液体复苏策略及存在的问题. 中华急诊医学杂志, 2013, 22(10): 1080-1083.
2. McGhan LJ, Jaroszewski DE. The role of toll-like receptor-4 in the development of multi-organ failure following traumatic haemorrhagic shock and resuscitation. Injury, 2012, 43(2): 129-136.
3. Zhang Q, Lu YQ, Jiang JK,et al. Early changes of CD4⁺CD25⁺Foxp3⁺ regulatory T cells and Th1/Th2, Tc1/Tc2 profiles in the peripheral blood of rats with controlled hemorrhagic shock and no fluid resuscitation. Chin Med J, 2012, 125(12): 2163-2167.
4. 沈璟, 王鹏飞, 王斌, 等. 限制性液体复苏对失血性休克大鼠肠损伤的影响. 中国普外基础与临床杂志, 2014, 21(6): 676-681.
5. Riha M, Kunio R, Van Y,et al. Uncontrolled hemorrhagic shock results in a hypercoagulable state modulated by initial fluid resuscitation regimens. J Trauma Acute Care Surg, 2013, 75(1): 129-134.
6. Vernon PJ, Paredes RM, Sooter AJ,et al. Severe hemorrhagic shock induces acute activation and expansion of IL-8⁺/IL-10⁺ neutrophils with enhanced oxidative reactivity in non-human primates. Shock, 2016, 46(3 Suppl 1): 129-136.
7. Cai Z, Falkensammer F, Andrukhov O,et al. Effects of shock waves on expression of IL-6, IL-8, MCP-1, and TNF-α expression by human periodontal ligament fibroblasts: anin vitro study. Med Sci Monit, 2016, 22(22): 914-921.
8. Boehm J, Fischer K, Bohnert M. Putative role of TNF-alpha, interleukin-8 and ICAM-1 as indicators of an early inflammatory reaction after burn: a morphological and immunohistochemical study of lung tissue of fire victims. J Clin Pathol, 2010, 63(11): 967-971.
9. 陈凛, 崔建新. 限制性液体复苏治疗创伤失血性休克争议与共识. 中国实用外科杂志, 2015, 35(2): 167-171.
10. Weiterer S, Uhle F, Siegler H,et al. Epigenetic regulation in sepsis : current state of knowledge. Anaesthesist, 2015, 64(1): 42-55.
11. Coletta C, Módis K, Oláh G,et al. Endothelial dysfunction is a potential contributor to multiple organ failure and mortality in aged mice subjected to septic shock: preclinical studies in a murine model of cecal ligation and puncture. Crit Care, 2014, 18(5): 511.
12. Binkowska AM, Michalak G, Słotwiński R. Current views on the mechanisms of immune responses to trauma and infection. Cent Eur J Immunol, 2015, 40(2): 206-216.
13. Jackman P. Immunomodulation in transfused trauma patients. Curr Opin Anaesthesiol, 2013, 26(2): 196-203.
14. Murdock AD, Berséus O, Hervig T,et al. Whole blood: the future of traumatic hemorrhagic shock resuscitation. Shock, 2014, 41(Suppl 1): 62-69.
15. Du Z, Jia H, Liu J,et al. Effects of three hydrogen-rich liquids on hemorrhagic shock in rats. J Surg Res, 2015, 193(1): 377-382.
16. Holcomb JB, Pati S. Optimal trauma resuscitation with plasma as the primary resuscitative fluid: the surgeon’s perspective. Hematology Am Soc Hematol Educ Program, 2013, 2013: 656-659.
17. Siwetz M, Blaschitz A, El-Heliebi A,et al. TNF-α alters the inflammatory secretion profile of human first trimester placenta. Lab Invest, 2016, 96(4): 428-438.
18. 董彪, 关维雨, 张德巍. 创伤失血性休克限制性液体复苏的治疗进展. 中国现代普通外科进展, 2014, 17(7): 576-579.
19. Kampmeier T, Rehberg S, Ertmer C,et al. Evolution of fluid therapy. Best Pract Res Clin Anaesthesiol, 2014, 28(3): 207-216.
20. Stancil A. Past and current trends in intravenous therapy for hemorrhagic shock. Air Med J, 2014, 33(4): 172-175.
21. Spahn DR, Bouillon B, Cerny V,et al. Management of bleeding and coagulopathy following major trauma: an updated European guideline. Crit Care, 2013, 17(2): R76.
22. Petroni RC, Biselli PJ, Lima TM,et al. Impact of time on fluid resuscitation with hypertonic saline (NaCl 7.5%) in rats with LPS-induced acute lung injury. Shock, 2015, 44(6): 609-615.
23. Godinho M, Padim P, Evora PR,et al. Curbing inflammation in hemorrhagic trauma: a review. Rev Col Bras Cir, 2015, 42(4): 273-278.
24. Delano J, Rizoli B, Rhind G,et al. Prehospital resuscitation of traumatic hemorrhagic shock with hypertonic solutions worsens hypocoagulation and hyperfibrinolysis. Shock, 2015, 44(1): 25-31.
25. Sordi R, Chiazza F, Johnson FL,et al. Inhibition of IκB kinase attenuates the organ injury and dysfunction associated with hemorrhagic shock. Mol Med, 2015, 21(21): 563-575.
26. Jeong KY, Suh GJ, Kwon WY,et al. The therapeutic effect and mechanism of niacin on acute lung injury in a rat model of hemorrhagic shock: Down-regulation of the reactive oxygen species-dependent nuclear factor κB pathway. J Trauma Acute Care Surg, 2015, 79(2): 247-255.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of different resuscitation fluids on interleukin-8 and tumor necrosis factor-α in rats with traumatic hemorrhagic shock

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