Antibody microarray analysis of the tissue apoptosis factors in patients with hepatic alveolar echinococcosis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SHAO Jun ^1,2 , WANG Zhixin ^1,2 ,  WANG Hu ^1,3 , LI Yanfei ^1,2 , ZHANG Lingqiang ^1,2 , ^1,2 , REN Li ^1,2 , HOU Lizhao ^1,2 , ZHOU Ying ^1,2 , WANG Haijiu ^1,2 ,  FAN Haining ^1,2

1. Medical College of Qinghai Universit, Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Qinghai University, Xining, Qinghai 810001, P.R.China;
2. Qinghai Province Key Laboratory of Hydatid Disease Researsh, Qinghai University Provincial Key Laboratory of Hydatid Disease, Xining, Qinghai 810001, P.R.China;
3. Qinghai Province Health & Family Planning Commission, Xining, Qinghai 810001, P.R.China;

Corresponding author：

WANG Hu, Email: whqhxn@126.com; FAN Haining, Email: fanhaining@medmail.com.cn

Keywords：

hepatic alveolar echinococcosis; apoptosis factor; antibody microarray

DOI：

10.7507/1007-9424.201608097

Video：

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Objective To detect and analize the expressions and it’s clinical significance of apoptosis factors in hepatic alveolar echinococcosis tissues by using antibody chip technology. Methods The liver tissue specimens (including the edge of lesions and normal liver tissues) of surgical resection of 6 patients with hepatic alveolar echinococcosis in Affiliated Hospital of Qinghai University were collected. The tissue protein was extracted and the level of apoptosis was detected by antibody chip technology. The data were analyzed by AAH-APO-G1 software. Results The expression levels of 5 kinds of apoptosis factors (Bad, Fas, IGFBP-3, P21 and XIAP) in the liver tissues of the marginal zone of hepatic alveolar echinococcosis were compared with that of the normal liver tissues, and the difference was statistically significant (P<0.05). The expression levels of Bad, Fas, IGFBP-3 and P21 were up-regulated, and the expression level of XIAP was down regulated. Conclusions Apoptosis-related factors play a role in the progression of the hepatic alveolar echinococcosis, there may be contact with the immune escape mechanisms, while promote apoptosis factor and inhibitory apoptosis factor that may exist the function imbalance, so more in-depth exploration the mechanism of apoptosis factors on hepatic alveolar echinococcosis in diagnosis and treatment have important significance.

Citation： SHAO Jun, WANG Zhixin, WANG Hu, LI Yanfei, ZHANG Lingqiang, REN Li, HOU Lizhao, ZHOU Ying, WANG Haijiu, FAN Haining. Antibody microarray analysis of the tissue apoptosis factors in patients with hepatic alveolar echinococcosis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2017, 24(4): 426-431. doi: 10.7507/1007-9424.201608097 Copy

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3.	赵玉辉, 贾智艳, 包红梅. 抗体芯片技术研究进展. 国际免疫学杂志, 2010, 33(3): 196-199.
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14.	Zhang T, Barclay L, Walensky LD, et al. Regulation of mitochondrial ceramide distribution by members of the BCL-2 family. J Lipid Res, 2015, 56(8):1501-1510.
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18.	Natesan M, Ulrich RG. Protein microarrays and biomarkers of infectious disease. Int J Mol Sci, 2010, 11(12): 5165-5183.
19.	春芃, 徐振山, 宋礼华. 抗体芯片技术在卵巢癌诊断中的应用进展.安徽医药, 2009, 13(8): 970-972.
20.	Santacatterina F, Chamorro M, de Arenas CN, et al. Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases. J Transl Med, 2015, 13: 65.
21.	何家璇, 薛荣亮. Bad 蛋白对细胞凋亡的调控作用. 医学综述, 2007, 13(3): 161-163.
22.	Matthews J M. Bcl-2 Inhibitors. Germany, Springer Berlin Heidelberg, 2013:184-184.
23.	孙建华, 李雁. Fas/FasL 系统在肿瘤细胞凋亡中的研究进展. 医学综述, 2015, 21(20): 3694-3696.
24.	Timmer T, de Vries EG, de Jong S. Fas receptor-mediated apoptosis: a clinical application? J Pathol, 2002, 196(2): 125-134.
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26.	Chen L, Park SM, Tumanov AV, et al. CD95 promotes tumourgrowth. Nature, 2010, 465(7297): 492-496.
27.	吴棪, 陈乔尔. 肿瘤免疫逃逸的相关分子及其作用机制研究. 安徽医药, 2016, 20(4): 621-625.
28.	李筠, 钟英斌, 卢玲, 等. 类胰岛素生长因子结合蛋白 IGFBP-3 的研究进展. 中国海洋大学学报(自然科学版), 2011, 41(9): 41-47.
29.	Butt AJ, Williams AC. IGFBP-3 and apoptosis—a licence to kill? Apoptosis, 2001, 6(3): 199-205 .
30.	Yamada PM, Lee KW. Perspectives in mammalian IGFBP-3 biology: localvs. systemic action. Am J Physiol Cell Physiol, 2009, 296(5): C954-C976.
31.	Jogie-Brahim S, Feldman D, Oh Y. Unraveling insulin-like growth factor binding protein-3 actions in human disease. Endocr Rev, 2009, 30(5): 417-437.
32.	王言飞, 刘玉芝, 李莲娜. P21 蛋白在食管鳞癌中表达的临床意义. 中华实用诊断与治疗杂志, 2008, 22(2): 108-109.
33.	张明, 孟和宝力高, 郭兰英, 等. p21 基因与肿瘤的研究进展. 中国煤炭工业医学杂志, 2011, 14(9): 1410-1411.
34.	Junxia W, Ping G, Yuan H, et al. Double strand RNA-guided endogeneous E-cadherin up-regulation induces the apoptosis and inhibits proliferation of breast carcinoma cells in vitro and in vivo. Cancer Science, 2010, 101(8): 1790-1796.
35.	Ren S, Kang MR, Wang J, et al. Targeted induction of endogenous NKX3-1 by small activating RNA inhibits prostate tumor growth. Prostate, 2013, 73(14): 1591-1601.
36.	Wu ZM, Dai C, Huang Y, et al. Anti-cancer effects of p21WAF1/CIP1 transcriptional activation induced by dsRNAs in human hepatocellular carcinoma cell lines. Acta Pharmacol Sin, 2011, 32(7): 939-946.
37.	陈雷, 尚培中. Xiap、Smac 与消化系肿瘤的研究进展. 中国现代普通外科进展, 2016, 19(4): 300-302.
38.	Zheng HC, Tsuneyama K, Takahashi H, et al. Aberrant Pim-3 expression is involved in gastric adenoma-adenocarcinoma sequence and cancer progression. J Cancer Res Clin Oncol, 2008, 134(4): 48l-488.
39.	Dubrez-Daloz L, Dupoux A, Cartier J. IAPS: More than just inhibitors of apoptosis proteins. Cell Cycle, 2008, 7(8): 1036-1046.

1. 张洋, 樊海宁. 泡型肝包虫病的治疗进展. 临床外科杂志, 2014, 22(7): 533-535.
2. 麦冉穆尼沙·吐尔逊, 邵英梅. 泡型肝包虫病诊治新进展. 腹部外科, 2015, 28(2): 137-139.
3. 赵玉辉, 贾智艳, 包红梅. 抗体芯片技术研究进展. 国际免疫学杂志, 2010, 33(3): 196-199.
4. Polat P, Kantarci M, Alper F,et al. Hydatid disease from head to toe. Radiographics, 2003, 23(2): 475-494, quiz 536-537.
5. Eckert J, Deplazes P. Biological, epidemiological, and clinical as 2 pects of echinococcosis, a zoonosis of increasing concern. ClinMicrobiol Rev, 2004, 17(1): 107-135.
6. 温浩, 徐明谦. 实用包虫病学.北京, 科学出版社, 2007: 308-308.
7. Kamiya M. Echinococcosis. Nihon Rinsho, 2007, 65 Suppl 3: 229-235.
8. 中国医师协会外科医师分会包虫病外科专业委员会. 肝两型包虫病诊断与治疗专家共识(2015版). 中华消化外科杂志, 2015, 14(4): 253-264.
9. 陈哲宇. 泡型肝包虫病的外科治疗现状. 中国普外基础与临床杂志, 2016, 23(5): 513-516.
10. 阎海. 细胞凋亡研究进展. 中山大学研究生学刊(自然科学、医学版), 2012, 33(3): 8-13.
11. 李春丰, 江清林, 庄树文. 细胞凋亡的研究进展. 亚太传统医药, 2010, 6(8): 162-163.
12. 唐珍, 易芳, 郭冬梅, 等. 细胞凋亡对衰老的调节在肿瘤的发生发展及治疗的研究进展.暨南大学学报(自然科学与医学版), 2016, 37(3): 215-220.
13. Zhang T, Saghatelian A. Emerging roles of lipids in BCL-2 family-regulated apoptosis. Biochim Biophys Acta, 2013, 1831(10): 1542-1554.
14. Zhang T, Barclay L, Walensky LD, et al. Regulation of mitochondrial ceramide distribution by members of the BCL-2 family. J Lipid Res, 2015, 56(8):1501-1510.
15. Berrade L, Garcia AE, Camarero JA. Protein microarrays: novel developments and applications. Pharm Res, 2011, 28(7): 1480-1499.
16. Sill M, Schröder C, Hoheisel JD, et al. Assessment and optimization of normalization methods for dual-colour antibody microarrays. BMC Bioinformatics, 2010, 11: 556.
17. 高东梅, 孙璐, 郭坤, 等. 多重定量抗体芯片检测系统在肝癌早期诊断中的应用. 中华肝脏病杂志, 2012, 20(10): 785-788.
18. Natesan M, Ulrich RG. Protein microarrays and biomarkers of infectious disease. Int J Mol Sci, 2010, 11(12): 5165-5183.
19. 春芃, 徐振山, 宋礼华. 抗体芯片技术在卵巢癌诊断中的应用进展.安徽医药, 2009, 13(8): 970-972.
20. Santacatterina F, Chamorro M, de Arenas CN, et al. Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases. J Transl Med, 2015, 13: 65.
21. 何家璇, 薛荣亮. Bad 蛋白对细胞凋亡的调控作用. 医学综述, 2007, 13(3): 161-163.
22. Matthews J M. Bcl-2 Inhibitors. Germany, Springer Berlin Heidelberg, 2013:184-184.
23. 孙建华, 李雁. Fas/FasL 系统在肿瘤细胞凋亡中的研究进展. 医学综述, 2015, 21(20): 3694-3696.
24. Timmer T, de Vries EG, de Jong S. Fas receptor-mediated apoptosis: a clinical application? J Pathol, 2002, 196(2): 125-134.
25. Strasser A, Jost PJ, Nagata S. The many roles of FAS receptor signaling in the immune system. Immunity, 2009, 30(2): 180-192.
26. Chen L, Park SM, Tumanov AV, et al. CD95 promotes tumourgrowth. Nature, 2010, 465(7297): 492-496.
27. 吴棪, 陈乔尔. 肿瘤免疫逃逸的相关分子及其作用机制研究. 安徽医药, 2016, 20(4): 621-625.
28. 李筠, 钟英斌, 卢玲, 等. 类胰岛素生长因子结合蛋白 IGFBP-3 的研究进展. 中国海洋大学学报(自然科学版), 2011, 41(9): 41-47.
29. Butt AJ, Williams AC. IGFBP-3 and apoptosis—a licence to kill? Apoptosis, 2001, 6(3): 199-205 .
30. Yamada PM, Lee KW. Perspectives in mammalian IGFBP-3 biology: localvs. systemic action. Am J Physiol Cell Physiol, 2009, 296(5): C954-C976.
31. Jogie-Brahim S, Feldman D, Oh Y. Unraveling insulin-like growth factor binding protein-3 actions in human disease. Endocr Rev, 2009, 30(5): 417-437.
32. 王言飞, 刘玉芝, 李莲娜. P21 蛋白在食管鳞癌中表达的临床意义. 中华实用诊断与治疗杂志, 2008, 22(2): 108-109.
33. 张明, 孟和宝力高, 郭兰英, 等. p21 基因与肿瘤的研究进展. 中国煤炭工业医学杂志, 2011, 14(9): 1410-1411.
34. Junxia W, Ping G, Yuan H, et al. Double strand RNA-guided endogeneous E-cadherin up-regulation induces the apoptosis and inhibits proliferation of breast carcinoma cells in vitro and in vivo. Cancer Science, 2010, 101(8): 1790-1796.
35. Ren S, Kang MR, Wang J, et al. Targeted induction of endogenous NKX3-1 by small activating RNA inhibits prostate tumor growth. Prostate, 2013, 73(14): 1591-1601.
36. Wu ZM, Dai C, Huang Y, et al. Anti-cancer effects of p21WAF1/CIP1 transcriptional activation induced by dsRNAs in human hepatocellular carcinoma cell lines. Acta Pharmacol Sin, 2011, 32(7): 939-946.
37. 陈雷, 尚培中. Xiap、Smac 与消化系肿瘤的研究进展. 中国现代普通外科进展, 2016, 19(4): 300-302.
38. Zheng HC, Tsuneyama K, Takahashi H, et al. Aberrant Pim-3 expression is involved in gastric adenoma-adenocarcinoma sequence and cancer progression. J Cancer Res Clin Oncol, 2008, 134(4): 48l-488.
39. Dubrez-Daloz L, Dupoux A, Cartier J. IAPS: More than just inhibitors of apoptosis proteins. Cell Cycle, 2008, 7(8): 1036-1046.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Antibody microarray analysis of the tissue apoptosis factors in patients with hepatic alveolar echinococcosis

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