Study of CD31 combined with D2-40 in peritoneal metastasis of synchronous colorectal cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHU Xinqiang ¹ ,  GUAN Wenxian ²

1. Department of General Surgery, Suqian People’s Hospital, Nanjing Drum Tower Hospital Group of Nanjing University Medical School, Suqian, Jiangsu 223800, P. R. China;
2. Department of General Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, P. R. China;

Corresponding author：

GUAN Wenxian, Email: 15850502391@163.com

Keywords：

CD31; D2-40; synchronous colorectal cancer; peritoneal metastasis

DOI：

10.7507/1007-9424.201706085

Video：

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Objective To detect expressions of CD31 and D2-40 in patients with simultaneous colorectal cancer with peritoneal metastasis and investigate their correlation between them. Method The expressions of CD31 and D2-40 protein in these 59 cases (there were 19 and 40 patients with or without peritoneal metastasis, respectively) of colorectal cancer were detected by immunohistochemical MaxVision method. Results ① The positive rates of CD31 and D2-40 protein expression in the colorectal cancer tissues with or without peritoneal metastasis were significantly higher than those of the normal colonic tissues (P<0.05), but which had no significant differences between the colorectal cancer tissues with or without peritoneal metastasis (P>0.05). ② The CD31 microvessel density (CD31-MVD) and D2-40 lymphatic microvessel density (D2-40-LMVD) in the colorectal cancer tissues with peritoneal metastasis were significantly higher than those of the normal colonic tissues (P<0.05) and the colorectal cancer tissues without peritoneal metastasis (P<0.05). ③ The CD31-MVD and D2-40-LMVD were not associated with all the clinicopathologic characteristics of the colorectal cancer patients without peritoneal metastasis (P>0.05), but which in the poorly differentiated adenocarcinoma and mucinous and signet ring cell carcinomas patients with peritoneal metastasis were significantly higher than those of the other types (P<0.05) and were not associated with the age, gender, and diamter of the tumor of it (P>0.05). ④ There was a positive correlation between the CD31-MVD and the D2-40-LMVD in the colorectal cancer patients with or without peritoneal metastasis (r=0.342, P=0.012; r=0.119, P=0.008). Conclusions CD31-MVD and D2-40-LMVD in colorectal cancer patients with peritoneal metastasis highly express and has a positive correlation, which means that they might have a certain relationship with peritoneal metastasis. There may be some common regulation way and collaboratively participate peritoneal metastasis of colorectal cancer.

Citation： ZHU Xinqiang, GUAN Wenxian. Study of CD31 combined with D2-40 in peritoneal metastasis of synchronous colorectal cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2017, 24(12): 1473-1479. doi: 10.7507/1007-9424.201706085 Copy

1.	朱信强, 管文贤. 结直肠癌同时性腹膜转移影响因素的多因素分析. 中国普外基础与临床杂志, 2016, 23(6): 696-701.
2.	王磊, 汪建平. 结直肠癌腹膜转移的研究现状及其争议. 中华实验外科杂志, 2014, 31(2): 234-235.
3.	Choi WW, Lewis MM, Lawson D, et al. Angiogenic and lymphangiogenic microvessel density in breast carcinoma: correlation with clinicopathologic parameters and VEGF-family gene expression. Mod Pathol, 2005, 18(1): 143-152.
4.	Weidner N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors. Breast Cancer Res Treat, 1995, 36(2): 169-180.
5.	唐颖, 王清峙, 赵然旭, 等. D2-40 和 CD31 在心海绵状血管瘤和淋巴管瘤中的表达及其鉴别诊断意义. 诊断病理学杂志, 2015, 22(1): 27-29.
6.	徐国萍, 张林, 苗杰, 等. 微血管密度与 CD105、CD31 在胃癌中的表达及意义. 胃肠病学和肝病学杂志, 2009, 18(6): 524-526.
7.	Newman DK, Fu G, Adams T, et al. The adhesion molecule PECAM-1 enhances the TGF-β-mediated inhibition of T cell function. Sci Signal, 2016, 9(418): ra27.
8.	Deliu IC, Neagoe CD, Beznă M, et al. Correlations between endothelial cell markers CD31, CD34 and CD105 in colorectal carcinoma. Rom J Morphol Embryol, 2016, 57(3): 1025-1030.
9.	Woodfin A, Voisin MB, Nourshargh S. PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol, 2007, 27(12): 2514-2523.
10.	Bazzoni G. Pathobiology of junctional adhesion molecules. Antioxid Redox Signal, 2011, 15(5): 1221-1234.
11.	Kuzu I, Bicknell R, Harris AL, et al. Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol, 1992, 45(2): 143-148.
12.	姜乃光, 陈晓江, 黄莺, 等. 胃癌 i-67 和 CD31 表达的免疫组化研究. 中国现代药物应用, 2009, 3(23): 94-95.
13.	李英, 张冬娅, 王艳芳, 等. 结直肠腺癌中 D2-40、CD34、VEGF 的表达及临床意义. 临床与实验病理学杂志, 2010, 26(2): 231-233.
14.	Kim OH, Kang GH, Noh H, et al. Proangiogenic TIE2(+)/CD31 (+) macrophages are the predominant population of tumor-associated macrophages infiltrating metastatic lymph nodes. Mol Cells, 2013, 36(5): 432-438.
15.	牟江洪, 阎晓初, 李增鹏, 等, 结直肠癌淋巴管生成的特点及其临床病理意义. 中华病理学杂志, 2005, 34(6): 348-352.
16.	Toll A, Gimeno-Beltrán J, Ferrandiz-Pulido C, et al. D2-40 immunohistochemical overexpression in cutaneous squamous cell carcinomas: a marker of metastatic risk. J Am Acad Dermatol, 2012, 67(6): 1310-1318.
17.	Jeon SR, Cho JY, Bok GH, et al. Does immunohistochemical staining have a clinical impact in early gastric cancer conducted endoscopic submucosal dissection? World J Gastroenterol, 2012, 18(33): 4578-4584.
18.	Yonemura Y, Endou Y, Tabachi K, et al. Evaluation of lymphatic invasion in primary gastric cancer by a new monoclonal antibody, D2-40. Hum Pathol, 2006, 37(9): 1193-1199.
19.	Takanami I. Lymphatic microvessel density using D2-40 is associated with nodal metastasis in non-small cell lung cancer. Oncol Rep, 2006, 15(2): 437-442.
20.	孙建军, 经巍, 倪艳艳, 等. 一种新的人结肠腺癌淋巴管生成裸鼠动物模型. 外科研究与新技术, 2012, 1(1): 49-55.
21.	Dimova I, Popivanov G, Djonov V. Angiogenesis in cancer—general pathways and their therapeutic implications. J BUON, 2014, 19(1): 15-21.
22.	Cacchi C, Arnholdt HM, Jähnig H, et al. Clinical significance of lymph vessel density in T3 colorectal carcinoma. Int J Colorectal Dis, 2012, 27(6): 721-726.
23.	Straume O, Jackson DG, Akslen L. AIndependent prognostic impact of lymphatic vessel density and presence of low-grade lymphangiogenesis in cutaneous melanoma. Clin Cancer Res, 2003, 9(1): 250-256.
24.	Weidner N, Semple JP, Welch WR, et al. Tumor angiogenesis and metastasis—correlation in invasive breast carcinoma. N Engl J Med, 1991, 324(1): 1-8.
25.	Weidner N. Tumor angiogenesis: review of current applications in tumor prognostication. Semin Diagn Pathol, 1993, 10(4): 302-313.
26.	岳彩霞, 马骥, 周海俊, 等. 蛋白酶体抑制剂 MG132 对 RhoA 诱导的肿瘤血管生成的抑制作用的研究. 四川大学学报(医学版), 2011, 42(4): 445-450.
27.	陆俭, 施琴, 陈康武, 等. 骶骨脊索瘤 CD40、PCNA、CD31 表达及其与肿瘤复发的相关性研究. 中国矫形外科杂志, 2011, 19(11): 1197-1200.
28.	Molavi D, Argani P. Distinguishing benign dissecting mucin (stromal mucin pools) from invasive mucinous carcinoma. Adv Anat Pathol, 2008, 15(1): 1-17.
29.	Legrier ME, de Pinieux G, Boyé K, et al. Mucinous differentiation features associated with hormonal escape in a human prostate cancer xenograft. Br J Cancer, 2004, 90(3): 720-727.
30.	Khattab AZ, Ahmed MI, Fouad MA, et al. Significance of p53 and CD31 in astrogliomas. Med Oncol, 2009, 26(1): 86-92.
31.	施华球, 鄢俊, 王祥财.VEGF-D 及其受体在结直肠癌中的表达及与淋巴管生成的关系. 山东医药, 2010, 50(1): 348-352.
32.	李鑫, 龙汉安, 肖秀丽, 等. D2-40 与 nm23 在结直肠癌中的表达及临床病理意义的探讨. 泸州医学院学报, 2014, 37(5): 456-460.
33.	田满福, 韩波. 检测 CA199、CA125、CA153 及 CEA 在肿瘤诊断中的意义. 临床和实验医学杂志, 2010, 9(7): 483-485.

1. 朱信强, 管文贤. 结直肠癌同时性腹膜转移影响因素的多因素分析. 中国普外基础与临床杂志, 2016, 23(6): 696-701.
2. 王磊, 汪建平. 结直肠癌腹膜转移的研究现状及其争议. 中华实验外科杂志, 2014, 31(2): 234-235.
3. Choi WW, Lewis MM, Lawson D, et al. Angiogenic and lymphangiogenic microvessel density in breast carcinoma: correlation with clinicopathologic parameters and VEGF-family gene expression. Mod Pathol, 2005, 18(1): 143-152.
4. Weidner N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors. Breast Cancer Res Treat, 1995, 36(2): 169-180.
5. 唐颖, 王清峙, 赵然旭, 等. D2-40 和 CD31 在心海绵状血管瘤和淋巴管瘤中的表达及其鉴别诊断意义. 诊断病理学杂志, 2015, 22(1): 27-29.
6. 徐国萍, 张林, 苗杰, 等. 微血管密度与 CD105、CD31 在胃癌中的表达及意义. 胃肠病学和肝病学杂志, 2009, 18(6): 524-526.
7. Newman DK, Fu G, Adams T, et al. The adhesion molecule PECAM-1 enhances the TGF-β-mediated inhibition of T cell function. Sci Signal, 2016, 9(418): ra27.
8. Deliu IC, Neagoe CD, Beznă M, et al. Correlations between endothelial cell markers CD31, CD34 and CD105 in colorectal carcinoma. Rom J Morphol Embryol, 2016, 57(3): 1025-1030.
9. Woodfin A, Voisin MB, Nourshargh S. PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol, 2007, 27(12): 2514-2523.
10. Bazzoni G. Pathobiology of junctional adhesion molecules. Antioxid Redox Signal, 2011, 15(5): 1221-1234.
11. Kuzu I, Bicknell R, Harris AL, et al. Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol, 1992, 45(2): 143-148.
12. 姜乃光, 陈晓江, 黄莺, 等. 胃癌 i-67 和 CD31 表达的免疫组化研究. 中国现代药物应用, 2009, 3(23): 94-95.
13. 李英, 张冬娅, 王艳芳, 等. 结直肠腺癌中 D2-40、CD34、VEGF 的表达及临床意义. 临床与实验病理学杂志, 2010, 26(2): 231-233.
14. Kim OH, Kang GH, Noh H, et al. Proangiogenic TIE2(+)/CD31 (+) macrophages are the predominant population of tumor-associated macrophages infiltrating metastatic lymph nodes. Mol Cells, 2013, 36(5): 432-438.
15. 牟江洪, 阎晓初, 李增鹏, 等, 结直肠癌淋巴管生成的特点及其临床病理意义. 中华病理学杂志, 2005, 34(6): 348-352.
16. Toll A, Gimeno-Beltrán J, Ferrandiz-Pulido C, et al. D2-40 immunohistochemical overexpression in cutaneous squamous cell carcinomas: a marker of metastatic risk. J Am Acad Dermatol, 2012, 67(6): 1310-1318.
17. Jeon SR, Cho JY, Bok GH, et al. Does immunohistochemical staining have a clinical impact in early gastric cancer conducted endoscopic submucosal dissection? World J Gastroenterol, 2012, 18(33): 4578-4584.
18. Yonemura Y, Endou Y, Tabachi K, et al. Evaluation of lymphatic invasion in primary gastric cancer by a new monoclonal antibody, D2-40. Hum Pathol, 2006, 37(9): 1193-1199.
19. Takanami I. Lymphatic microvessel density using D2-40 is associated with nodal metastasis in non-small cell lung cancer. Oncol Rep, 2006, 15(2): 437-442.
20. 孙建军, 经巍, 倪艳艳, 等. 一种新的人结肠腺癌淋巴管生成裸鼠动物模型. 外科研究与新技术, 2012, 1(1): 49-55.
21. Dimova I, Popivanov G, Djonov V. Angiogenesis in cancer—general pathways and their therapeutic implications. J BUON, 2014, 19(1): 15-21.
22. Cacchi C, Arnholdt HM, Jähnig H, et al. Clinical significance of lymph vessel density in T3 colorectal carcinoma. Int J Colorectal Dis, 2012, 27(6): 721-726.
23. Straume O, Jackson DG, Akslen L. AIndependent prognostic impact of lymphatic vessel density and presence of low-grade lymphangiogenesis in cutaneous melanoma. Clin Cancer Res, 2003, 9(1): 250-256.
24. Weidner N, Semple JP, Welch WR, et al. Tumor angiogenesis and metastasis—correlation in invasive breast carcinoma. N Engl J Med, 1991, 324(1): 1-8.
25. Weidner N. Tumor angiogenesis: review of current applications in tumor prognostication. Semin Diagn Pathol, 1993, 10(4): 302-313.
26. 岳彩霞, 马骥, 周海俊, 等. 蛋白酶体抑制剂 MG132 对 RhoA 诱导的肿瘤血管生成的抑制作用的研究. 四川大学学报(医学版), 2011, 42(4): 445-450.
27. 陆俭, 施琴, 陈康武, 等. 骶骨脊索瘤 CD40、PCNA、CD31 表达及其与肿瘤复发的相关性研究. 中国矫形外科杂志, 2011, 19(11): 1197-1200.
28. Molavi D, Argani P. Distinguishing benign dissecting mucin (stromal mucin pools) from invasive mucinous carcinoma. Adv Anat Pathol, 2008, 15(1): 1-17.
29. Legrier ME, de Pinieux G, Boyé K, et al. Mucinous differentiation features associated with hormonal escape in a human prostate cancer xenograft. Br J Cancer, 2004, 90(3): 720-727.
30. Khattab AZ, Ahmed MI, Fouad MA, et al. Significance of p53 and CD31 in astrogliomas. Med Oncol, 2009, 26(1): 86-92.
31. 施华球, 鄢俊, 王祥财.VEGF-D 及其受体在结直肠癌中的表达及与淋巴管生成的关系. 山东医药, 2010, 50(1): 348-352.
32. 李鑫, 龙汉安, 肖秀丽, 等. D2-40 与 nm23 在结直肠癌中的表达及临床病理意义的探讨. 泸州医学院学报, 2014, 37(5): 456-460.
33. 田满福, 韩波. 检测 CA199、CA125、CA153 及 CEA 在肿瘤诊断中的意义. 临床和实验医学杂志, 2010, 9(7): 483-485.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study of CD31 combined with D2-40 in peritoneal metastasis of synchronous colorectal cancer

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