Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Yang ¹ ,  YAO Dianbo ² , WU Shuodong ²

1. Department of Pathology, Shenyang DI’AN Medical Testing Company Limited, Shenyang 110041, P. R. China;
2. Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, P. R. China;

Corresponding author：

YAO Dianbo, Email: yaodianbo@163.com

Keywords：

biliary pigment stone; curcumin; lipopolysaccharide; β-glucuronidase; c-myc protein

DOI：

10.7507/1007-9424.201903082

Video：

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Objective To investigate the effect of curcumin on the expression regulation of endogenousβ-glucoronidase (β-GD) induced by lipopolysaccharide (LPS).Methods ① Human normal intrahepatic biliary epithelial cell line (HiBEpiC) cells in the logarithmic growth phase were divided into blank control group (0 h group) and 7 different stimulation time groups. The cell density was adjusted to 1×10⁴/mL, and the cells were stimulated with 100 mg/mL LPS for 1, 3, 6, 18, and 24 hours respectively, including another two groups where the cells were cultured with LPS-free medium for 18 and 24 hours after LPS stimulation for 24 h. ② HiBEpiC cells in the logarithmic growth phase were divided into blank control group, LPS+low, medium, and high concentration curcumin group. The cell density was adjusted to 1×10⁴/mL. In the blank control group, cells were not stimulated with any reagent; in the LPS group, cells were stimulated with 100 mg/mL LPS, in the other three groups, the cells were stimulated with 100 mg/mL LPS and simultaneously 20, 40, and 80 μmol/L curcumin, respectively, for 24 hours. The expressions of c-myc and endogenous β-GD were detected by Western blot method.Results ① The expressions of endogenous β-GD and c-myc in HiBEpiC cells gradually increased with the prolongation of treatment time by LPS, and the expression levels of β-GD and c-myc at each time point group were significantly different from those in the 0 h group (P<0.05). ② There were significant difference between any two groups of the blank control group, LPS group, LPS+low concentration of curcumin group, LPS+medium concentration of curcumin group, and LPS+high concentration of curcumin group (P<0.05).Conclusion Curcumin is able to inhibit the increased expression of endogenous β-GD induced by LPS, possibly via inhibiting expression of c-myc.

Citation： WANG Yang, YAO Dianbo, WU Shuodong. Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(8): 917-922. doi: 10.7507/1007-9424.201903082 Copy

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14.	苏洋, 吴硕东, 金俊哲, 等. 肠屏障功能对豚鼠胆囊胆色素结石形成的影响. 世界华人消化杂志, 2005, 13(24): 2853-2857.
15.	崔东旭, 孙韶龙, 张小薄, 等. 内毒素调节血细胞释放β-葡萄糖苷酸酶的实验研究. 中国现代医学杂志, 2008, 18(3): 283-285.
16.	孙韶龙, 吴硕东, 戴显伟, 等. 内毒素调节肝脏β-葡萄糖苷酸酶mRNA的表达. 世界华人消化杂志, 2007, 15(17): 1887-1892.
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19.	Hao Q, Chen X, Wang X, et al. Curcumin attenuates angiotensin Ⅱ-induced abdominal aortic aneurysm by inhibition of inflammatory response and ERK signaling pathways. Evid Based Complement Alternat Med, 2014, 2014: 270930.
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25.	李强, 王斌生, 柴琛. 姜黄素诱导胃癌细胞凋亡中MicroRNA表达谱的分析. 医药前沿, 2016, 6(17): 160-162.
26.	周银霞, 张崇, 傅海珍, 等. 姜黄素类组分通过下调TLR4/NF-κB通路抑制LPS诱导的血管内皮细胞损伤. 中南药学, 2018, 16(11): 1521-1525.
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29.	Yang Q, Wu Z, Liu F, et al. Effective treatment of chronic proliferative cholangitis by local gentamicin infusion in rabbits. Biomed Res Int, 2018, 2018: 6751952.

1. Tazuma S. Gallstone disease: epidemiology, pathogenesis, and classification of biliary stones (common bile duct and intrahepatic). Best Pract Res Clin Gastroenterol, 2006, 20(6): 1075-1083.
2. 中华医学会外科学分会胆道外科学组. 肝胆管结石病诊断治疗指南. 中华消化外科杂志, 2007, 6(2): 156-161.
3. 吴硕东, 于宏, 苏洋, 等. 原发性胆管色素结石形成原因的阶段性研究总结. 中华肝胆外科杂志, 2007, 13(8): 515-518.
4. Wang Y, Qi M, Qin C, et al. Role of the biliary microbiome in gallstone disease. Expert Rev Gastroenterol Hepatol, 2018, 12(12): 1193-1205.
5. 张西波, 崔乃强, 李东华. 清热利湿方防治兔胆色素结石形成的实验研究. 中国中西医结合杂志, 2007, 27(3): 241-243.
6. 姚殿波, 邓天麟, 柯昌伟, 等. 内源性β-葡萄糖醛酸酶表达在肝内胆管结石形成中的作用. 中华肝胆外科杂志, 2017, 23(7): 460-463.
7. Notoya M, Nishimura H, Woo JT, et al. Curcumin inhibits the proliferation and mineralization of cultured osteoblasts. Eur J Pharmacol, 2006, 534(1-3): 55-62.
8. Zhu M, Zheng Z, Huang J, et al. Modulation of miR-34a in curcumin-induced antiproliferation of prostate cancer cells. J Cell Biochem, 2019, [Epub ahead of print].
9. Wang JY, Wang X, Wang XJ, et al. Curcumin inhibits the growth via Wnt/β-catenin pathway in non-small-cell lung cancer cells. Eur Rev Med Pharmacol Sci, 2018, 22(21): 7492-7499.
10. Naz H, Islam A, Waheed A, et al. Human β-glucuronidase: structure, function, and application in enzyme replacement therapy. Rejuvenation Res, 2013, 16(5): 352-363.
11. Maki T. Pathogenesis of calcium bilirubinate gallstone: role ofE. coli, beta-glucuronidase and coagulation by inorganic ions, polyelectrolytes and agitation. Ann Surg, 1966, 164(1): 90-100.
12. Ma WJ, Zhou Y, Shrestha A, et al. Applying chemical bile duct embolization to achieve chemical hepatectomy in hepatolithiasis: a further experimental study. J Surg Res, 2014, 187(1): 113-121.
13. Li F, Cheng J, He S, et al. The practical value of applying chemical biliary duct embolization to chemical hepatectomy for treatment of hepatolithiasis. J Surg Res, 2005, 127(2): 131-138.
14. 苏洋, 吴硕东, 金俊哲, 等. 肠屏障功能对豚鼠胆囊胆色素结石形成的影响. 世界华人消化杂志, 2005, 13(24): 2853-2857.
15. 崔东旭, 孙韶龙, 张小薄, 等. 内毒素调节血细胞释放β-葡萄糖苷酸酶的实验研究. 中国现代医学杂志, 2008, 18(3): 283-285.
16. 孙韶龙, 吴硕东, 戴显伟, 等. 内毒素调节肝脏β-葡萄糖苷酸酶mRNA的表达. 世界华人消化杂志, 2007, 15(17): 1887-1892.
17. Yao D, Dong Q, Tian Y, et al. Lipopolysaccharide stimulates endogenous β-glucuronidase via PKC/NF-κB/c-myc signaling cascade: a possible factor in hepatolithiasis formation. Mol Cell Biochem, 2018, 444(1-2): 93-102.
18. Soliman MM, Abdo Nassan M, Ismail TA. Immunohistochemical and molecular study on the protective effect of curcumin against hepatic toxicity induced by paracetamol in Wistar rats. BMC Complement Altern Med, 2014, 14: 457.
19. Hao Q, Chen X, Wang X, et al. Curcumin attenuates angiotensin Ⅱ-induced abdominal aortic aneurysm by inhibition of inflammatory response and ERK signaling pathways. Evid Based Complement Alternat Med, 2014, 2014: 270930.
20. Guo LD, Shen YQ, Zhao XH, et al. Curcumin combined with oxaliplatin effectively suppress colorectal carcinoma in vivo through inducing apoptosis. Phytother Res, 2015, 29(3): 357-365.
21. Liu G, Xiang T, Wu QF, et al. Curcumin suppresses the proliferation of gastric cancer cells by downregulating H19. Oncol Lett, 2016, 12(6): 5156-5162.
22. Zheng R, Deng Q, Liu Y, et al. Curcumin inhibits gastric carcinoma cell growth and induces apoptosis by suppressing the Wnt/β-Catenin signaling pathway. Med Sci Monit, 2017, 23: 163-171.
23. Kong YW, Cannell IG, de Moor CH, et al. The mechanism of micro-RNA-mediated translation repression is determined by the promoter of the target gene. Proc Natl Acad Sci U S A, 2008, 105(26): 8866-8871.
24. Li J, Wang K, Chen X, et al. Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells. BMC Mol Biol, 2012, 13: 4.
25. 李强, 王斌生, 柴琛. 姜黄素诱导胃癌细胞凋亡中MicroRNA表达谱的分析. 医药前沿, 2016, 6(17): 160-162.
26. 周银霞, 张崇, 傅海珍, 等. 姜黄素类组分通过下调TLR4/NF-κB通路抑制LPS诱导的血管内皮细胞损伤. 中南药学, 2018, 16(11): 1521-1525.
27. Bandgar BP, Kinkar SN, Chavan HV, et al. Synthesis and biological evaluation of asymmetric indole curcumin analogs as potential anti-inflammatory and antioxidant agents. J Enzyme Inhib Med Chem, 2014, 29(1): 7-11.
28. Yang Q, Wang J, Liu F, et al. A novel rabbit model for benign biliary stricture formation and the effects of medication infusions on stricture formation. Dig Dis Sci, 2018, 63(10): 2653-2661.
29. Yang Q, Wu Z, Liu F, et al. Effective treatment of chronic proliferative cholangitis by local gentamicin infusion in rabbits. Biomed Res Int, 2018, 2018: 6751952.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression

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