An improved method for calculating liver volume in partial hepatectomy in rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HE Yutao ^1,2 , FENG Shiming ^1,2 , ZHOU Tao ^1,2 ,  RAN Jianghua ^1,2 , LI Wang ¹ , LI Xiaokai ¹ , DONG Yun ^1,2 , WU Ruichao ¹ , ZHANG Xibing ¹ , ZHENG Kepu ¹

1. Department of Hepatobiliary and Pancreatic Surgery, Calmete Hospital Affiliated to Kunming Medical University, Kunming 650000, P. R. China;
2. The First People's Hospital of Kunming, Kunming 650000, P. R. China;

Corresponding author：

RAN Jianghua, Email: rih2u@163.com

Keywords：

partial hepatectomy; whole liver volume; residual liver volume; liver volume calculation; indirect method

DOI：

10.7507/1007-9424.201911056

Video：

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Objective To improve the calculation method of the sum of residual hepatic lobe volume and total liver volume after partial hepatectomy in rats.Methods One hundred and thirty-five SD rats of different body sizes were divided into five groups by completely random design. The body length, tail length, chest circumference, body weight and length of hepatic triangle lobe of the rats were measured before surgery. Then, according to the classic Higgins and Anderson methods, different lobectomies of liver were performed for each group: middle lobe + left inner lobe, left outer lobe, bilateral papillary lobe, triangular lobe, and right lateral lobe were removed; the proportion of theoretical liver resection in each group was 38.1%, 30.1%, 7.9%, 7.8% and 15.3%, respectively. The actual liver resection volume and residual liver volume were measured after surgery. we finally analyzed statistical differences of liver volume calculated by different indirect methods. In addition, the correlation analysis and regression analysis were conducted between the preoperative measured rat body surface parameters and the measured whole liver volume, so as to explore a more simple and accurate volume measurement method.Results The actual proportion of liver resection in each group was 35.0%, 29.2%, 7.1%, 4.9% and 12.0%, respectively. Compared with the residual liver lobe volume actually measured, that calculated by using the indirect method of substitution of the theoretical liver resection proportion was statistically different in all the other four groups except the left outer lobe group. However, there was no statistical difference between the residual liver lobe volume actually measured and that calculated by the actual liver resection proportion in the 5 groups. In addition, in the preoperative measurement of 5 kinds of basic parameters of rats, the body length has the best correlation with whole liver volume, and the regression equation is .Conclusions The liver volume calculated by indirect method using the actual liver resection proportion, compared with the theoretical liver resection proportion, is more accurate. Compared with the body weight, the body length has the better correlation with whole liver volume.

Citation： HE Yutao, FENG Shiming, ZHOU Tao, RAN Jianghua, LI Wang, LI Xiaokai, DONG Yun, WU Ruichao, ZHANG Xibing, ZHENG Kepu. An improved method for calculating liver volume in partial hepatectomy in rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2020, 27(8): 935-939. doi: 10.7507/1007-9424.201911056 Copy

1.	Guo SL, Su LN, Zhai YN, et al. The clinical value of hepatic extracellular volume fraction using routine multiphasic contrast-enhanced liver CT for staging liver fibrosis. Clin Radiol, 2017, 72(3): 242-246.
2.	Luetkens JA, Klein S, Traber F, et al. Quantification of Liver Fibrosis at T1 and T2 Mapping with Extracellular Volume Fraction MRI: Preclinical Results. Radiology, 2018, 288(3): 748-754.
3.	史冀华, 朱盛兴, 张水军. 大鼠肝部分切除术的应用解剖及实施. 世界华人消化杂志, 2008, 16(22): 2516-2520.
4.	Butcher LM, Ito M, Brimpari M, et al. Non-CG DNA methylation is a biomarker for assessing endodermal differentiation capacity in pluripotent stem cells. Nat Commun, 2016, 7: 10458.
5.	Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat. Surgery, 1983, 93(1 Pt 1): 64-69.
6.	Moreira MC, Azevedo IM, Oliveira CN, et al. Influence of the colon in liver regeneration of rats submitted to hepatectomy and colectomy. Rev Col Bras Cir, 2017, 44(5): 476-481.
7.	Michalopoulos GK. Hepatostat: liver regeneration and normal liver tissue maintenance. Hepatology, 2017, 65(4): 1384-1392.
8.	Fontana J, Kucera O, Mezera V, et al. Glucagon-like peptide-1 analogues exenatide and liraglutide exert inhibitory effect on the early phase of liver regeneration after partial hepatectomy in rats. Physiol Res, 2017, 66(5): 833-844.
9.	Tsai JM, Weissman IL, Rinkevich Y. Partial lobular hepatectomy: a surgical model for morphologic liver regeneration. J Vis Exp, 2018, 135: 57302.
10.	Li H, Ge X, Pan K, et al. The predictive role of tenascin-C and cellular communication network factor 3 (CCN3) in post hepatectomy liver failure in a rat model and 50 patients following partial hepatectomy. Med Sci Monit, 2019, 25: 6755-6766.
11.	Rahbari NN, Garden OJ, Padbury R, et al. Posthepatectomy liver failure: a definition and grading by the International Study Group of Liver Surgery (ISGLS). Surgery, 2011, 149(5): 713-724.
12.	Gilg S, Sandstrom P, Rizell M, et al. The impact of post-hepatectomy liver failure on mortality: a population-based study. Scand J Gastroenterol, 2018, 53(10-11): 1335-1339.
13.	Andreatos N, Amini N, Gani F, et al. Albumin-bilirubin score: predicting short-term outcomes including bile leak and post-hepatectomy liver failure following hepatic resection. J Gastrointest Surg, 2017, 21(2): 238-248.
14.	Shi J, Han X, Wang J, et al. Matrine prevents the early development of hepatocellular carcinoma like lesions in rat liver. Exp Ther Med, 2019, 18(4): 2583-2591.
15.	Xiang L, Li J, Chen J, et al. Prospective cohort study of laparoscopic and open hepatectomy for hepatocellular carcinoma. Br J Surg, 2016, 103(13): 1895-1901.
16.	Kong J, Shen S, Wang W. Synchronous hepatectomy and splenectomy vs hepatectomy for selected patients with hepatocellular carcinoma and clinically significant portal hypertension: A systematic review and meta-analysis. J Surg Oncol, 2019, 119(7): 964-973.
17.	Shen J, Li C, Yan L, et al. Short-and long-term outcomes between young and older HCC patients exceeding the Milan criteria after hepatectomy. Ann Hepatol, 2018, 17(1): 134-143.
18.	Uchiyama H, Harimoto N, Itoh S, et al. Pleural effusion after hepatectomy for hepatocellular carcinoma: risk factor analyses and its impact on oncological outcomes. World J Surg, 2017, 41(4): 1089-1099.
19.	Lim C, Salloum C, Osseis M, et al. Short-term outcomes following hepatectomy for hepatocellular carcinoma within and beyond the BCLC guidelines: A prospective study. HPB (Oxford), 2018, 20(3): 222-230.
20.	柴军武, 蒋文涛, 王辉, 等. 体内外联合切除法在大鼠 30% 减体积肝移植中的应用. 中华实验外科杂志, 2009, 26(8): 1082.
21.	Xia R, Emond JC. Orthotopic partial liver transplantation in the rat: a model of 70% hepatectomy and reduced size liver transplantation. Transplantation, 1993, 56(4): 1041-1043.
22.	Guo L, Haga S, Enosawa S, et al. Improved hepatic regeneration with reduced injury by redox factor-1 in a rat small-sized liver transplant model. Am J Transplant, 2004, 4(6): 879-887.
23.	郭源, 蔡金贞, 罗毅, 等. 原位劈离式供肝儿童肝移植经验总结. 中华器官移植杂志, 2019, 40(1): 18-21.
24.	Reichman TW, Sandroussi C, Azouz SM, et al. Living donor hepatectomy: the importance of the residual liver volume. Liver Transpl, 2011, 17(12): 1404-1411.
25.	黄明, 秦雪琴, 陈悦, 等. 红景天苷对家兔 50%减体积原位肝移植术后疲劳综合征的效果. 青岛大学医学院学报, 2017, 53(3): 269-271, 275.
26.	吕毅, 潘承恩, 孟令忠, 等. SD 大鼠肝脏及附属管道的应用解剖. 西安医科大学学报 (中文版), 1998, 19(1): 70-73.
27.	陆森, 钱建民, 黄海清, 等. 几种不同预测大鼠肝脏体积 (重量) 方法的比较. 中华实验外科杂志, 2002, 19(3): 252-253.

1. Guo SL, Su LN, Zhai YN, et al. The clinical value of hepatic extracellular volume fraction using routine multiphasic contrast-enhanced liver CT for staging liver fibrosis. Clin Radiol, 2017, 72(3): 242-246.
2. Luetkens JA, Klein S, Traber F, et al. Quantification of Liver Fibrosis at T1 and T2 Mapping with Extracellular Volume Fraction MRI: Preclinical Results. Radiology, 2018, 288(3): 748-754.
3. 史冀华, 朱盛兴, 张水军. 大鼠肝部分切除术的应用解剖及实施. 世界华人消化杂志, 2008, 16(22): 2516-2520.
4. Butcher LM, Ito M, Brimpari M, et al. Non-CG DNA methylation is a biomarker for assessing endodermal differentiation capacity in pluripotent stem cells. Nat Commun, 2016, 7: 10458.
5. Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat. Surgery, 1983, 93(1 Pt 1): 64-69.
6. Moreira MC, Azevedo IM, Oliveira CN, et al. Influence of the colon in liver regeneration of rats submitted to hepatectomy and colectomy. Rev Col Bras Cir, 2017, 44(5): 476-481.
7. Michalopoulos GK. Hepatostat: liver regeneration and normal liver tissue maintenance. Hepatology, 2017, 65(4): 1384-1392.
8. Fontana J, Kucera O, Mezera V, et al. Glucagon-like peptide-1 analogues exenatide and liraglutide exert inhibitory effect on the early phase of liver regeneration after partial hepatectomy in rats. Physiol Res, 2017, 66(5): 833-844.
9. Tsai JM, Weissman IL, Rinkevich Y. Partial lobular hepatectomy: a surgical model for morphologic liver regeneration. J Vis Exp, 2018, 135: 57302.
10. Li H, Ge X, Pan K, et al. The predictive role of tenascin-C and cellular communication network factor 3 (CCN3) in post hepatectomy liver failure in a rat model and 50 patients following partial hepatectomy. Med Sci Monit, 2019, 25: 6755-6766.
11. Rahbari NN, Garden OJ, Padbury R, et al. Posthepatectomy liver failure: a definition and grading by the International Study Group of Liver Surgery (ISGLS). Surgery, 2011, 149(5): 713-724.
12. Gilg S, Sandstrom P, Rizell M, et al. The impact of post-hepatectomy liver failure on mortality: a population-based study. Scand J Gastroenterol, 2018, 53(10-11): 1335-1339.
13. Andreatos N, Amini N, Gani F, et al. Albumin-bilirubin score: predicting short-term outcomes including bile leak and post-hepatectomy liver failure following hepatic resection. J Gastrointest Surg, 2017, 21(2): 238-248.
14. Shi J, Han X, Wang J, et al. Matrine prevents the early development of hepatocellular carcinoma like lesions in rat liver. Exp Ther Med, 2019, 18(4): 2583-2591.
15. Xiang L, Li J, Chen J, et al. Prospective cohort study of laparoscopic and open hepatectomy for hepatocellular carcinoma. Br J Surg, 2016, 103(13): 1895-1901.
16. Kong J, Shen S, Wang W. Synchronous hepatectomy and splenectomy vs hepatectomy for selected patients with hepatocellular carcinoma and clinically significant portal hypertension: A systematic review and meta-analysis. J Surg Oncol, 2019, 119(7): 964-973.
17. Shen J, Li C, Yan L, et al. Short-and long-term outcomes between young and older HCC patients exceeding the Milan criteria after hepatectomy. Ann Hepatol, 2018, 17(1): 134-143.
18. Uchiyama H, Harimoto N, Itoh S, et al. Pleural effusion after hepatectomy for hepatocellular carcinoma: risk factor analyses and its impact on oncological outcomes. World J Surg, 2017, 41(4): 1089-1099.
19. Lim C, Salloum C, Osseis M, et al. Short-term outcomes following hepatectomy for hepatocellular carcinoma within and beyond the BCLC guidelines: A prospective study. HPB (Oxford), 2018, 20(3): 222-230.
20. 柴军武, 蒋文涛, 王辉, 等. 体内外联合切除法在大鼠 30% 减体积肝移植中的应用. 中华实验外科杂志, 2009, 26(8): 1082.
21. Xia R, Emond JC. Orthotopic partial liver transplantation in the rat: a model of 70% hepatectomy and reduced size liver transplantation. Transplantation, 1993, 56(4): 1041-1043.
22. Guo L, Haga S, Enosawa S, et al. Improved hepatic regeneration with reduced injury by redox factor-1 in a rat small-sized liver transplant model. Am J Transplant, 2004, 4(6): 879-887.
23. 郭源, 蔡金贞, 罗毅, 等. 原位劈离式供肝儿童肝移植经验总结. 中华器官移植杂志, 2019, 40(1): 18-21.
24. Reichman TW, Sandroussi C, Azouz SM, et al. Living donor hepatectomy: the importance of the residual liver volume. Liver Transpl, 2011, 17(12): 1404-1411.
25. 黄明, 秦雪琴, 陈悦, 等. 红景天苷对家兔 50%减体积原位肝移植术后疲劳综合征的效果. 青岛大学医学院学报, 2017, 53(3): 269-271, 275.
26. 吕毅, 潘承恩, 孟令忠, 等. SD 大鼠肝脏及附属管道的应用解剖. 西安医科大学学报 (中文版), 1998, 19(1): 70-73.
27. 陆森, 钱建民, 黄海清, 等. 几种不同预测大鼠肝脏体积 (重量) 方法的比较. 中华实验外科杂志, 2002, 19(3): 252-253.

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An improved method for calculating liver volume in partial hepatectomy in rats

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