Significance of the expression of VEGF in postoperative portal venous thrombosis in patients with portal hypertension_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Jun , HU Hongsheng , WU Hongwei , FU Haifeng , WEI Guangmin , HU Wei ,  SUN Shaohua

Department of Hepatopancreatobiliary, Breast and Thyroid Surgery, Dongfeng Hospital Affiliated to Hubei Medicial College, Shiyan, Hubei 442008, P. R. China;

Corresponding author：

SUN Shaohua, Email: ssh.2222@163.com

Keywords：

portal hypertension; thrombosis; vascular endothelial growth factor; platelet

DOI：

10.7507/1007-9424.202004072

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the significance of the expression of vascular endothelial growth factor (VEGF) in portal vein thrombosis after operation in patients with portal hypertension.Methods The serum of 146 patients with portal hypertension treated in Dongfeng Hospital Affiliated to Hubei Medicial College from January 2014 to December 2018 and the surgically removed splenic vein and spleen specimens were collected. The serum VEGF level was determined by enzyme-linked immunosorbent assay, and the expressions of VEGF in splenic vein and spleen tissues were detected by immunohistochemistry. According to whether portal vein thrombosis was formed after operation, the patients were divided into thrombosis group and non-thrombosis group, and the differences between the groups were compared.Results The serum VEGF level in the thrombosis group was significantly higher than that in the non-thrombosis group (P<0.05). In splenic vein wall and spleen tissues, VEGF staining indexes in the thrombosis group were significantly higher than those in the non-thrombosis group (P<0.05).Conclusions Postoperative portal vein thrombosis in patients with portal hypertension may be related to the serum VEGF level. The high expressions of VEGF in splenic vein wall and spleen suggest that VEGF may participate in the formation process of portal vein thrombosis.

Citation： LIU Jun, HU Hongsheng, WU Hongwei, FU Haifeng, WEI Guangmin, HU Wei, SUN Shaohua. Significance of the expression of VEGF in postoperative portal venous thrombosis in patients with portal hypertension. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2021, 28(1): 63-66. doi: 10.7507/1007-9424.202004072 Copy

1.	刘军, 曲凯, 万永, 等. 门静脉压力无创测量方法在门静脉高压症诊疗中的应用. 中华普通外科杂志, 2013, 28(2): 164-166.
2.	中华外科学分会门静脉高压症学组. 肝硬化门静脉高压症食管胃底静脉曲张破裂出血的诊治共识 (2015 版). 中华普通外科杂志, 2016, 31(2): 167-170.
3.	Kuroki T, Kitasato A, Tokunaga T, et al. Predictors of portal and splenic vein thrombosis after laparoscopic splenectomy: a retrospective analysis of a single-center experience. Surg today, 2018, 48(8): 804-809.
4.	Hirashita T, Ohta M, Kai S, et al. Implications of portal vein thrombosis after splenectomy for patients with idiopathic portal hypertension. Surg Today, 2011, 41(11): 1475-1480.
5.	尚玉清, 朱虹霖. 门体断流术治疗门静脉高压症后门静脉系统血栓形成的危险因素分析. 中国普外基础与临床杂志, 2016, 23(12): 1488-1491.
6.	Basit SA, Stone CD, Gish R. Portal vein thrombosis. Clin Liver Dis, 2015, 19(1): 199-221.
7.	戴朝六, 徐锋, 金添强. 重视门静脉高压症脾切除断流术后门静脉系统血栓的诊治. 中华普通外科学文献 (电子版), 2019, 13(3): 169-174.
8.	Verheul HM, Jorna AS, Hoekman K, et al. Vascular endothelial growth factor-stimulated endothelial cells promote adhesion and activation of platelets. Blood, 2000, 96(13): 4216-4221.
9.	Nosaka M, Ishida Y, Kimura A, et al. Absence of IFN-γ accelerates thrombus resolution through enhanced MMP-9 and VEGF expression in mice. J Clin Invest, 2011, 121(7): 2911-2920.
10.	Vermeersch E, Denorme F, Maes W, et al. The role of platelet and endothelial GARP in thrombosis and hemostasis. PloS One, 2017, 12(3): e0173329.
11.	Vogel S, Thein SL. Platelets at the crossroads of thrombosis, inflammation and haemolysis. Br J Haematol, 2018, 180(5): 761-767.
12.	Shim JW, Madsen JR. VEGF signaling in neurological disorders. Int J Mol Sci, 2018, 19(1): 275.
13.	Dalal ElSayed Abdelaal. Vascular endothelial growth factor in diagnosis of pleural effusion. Egypt J Chest Dis Tuberc, 2017, 66(1): 115-119.
14.	Möhle R, Green D, Moore MA, et al. Constitutive production and thrombin-induced release of vascular endothelial growth factor by human megakaryocytes and platelets. Proc Natl Acad Sci U S A, 1997, 94(2): 663-668.
15.	O’Byrne KJ, Dobbs N, Propper D, et al. Vascular endothelial growth factor platelet counts, and prognosis in renal cancer. Lancet, 1999, 353(9163): 1494-1495.
16.	Kim SJ, Choi IK, Park KH, et al. Serum vascular endothelial growth factor per platelet count in hepatocellular carcinoma: correlations with clinical parameters and survival. Jpn J Clin Oncol, 2004, 34(4): 184-190.
17.	郑波, 廖东旭, 杨训, 等. 门静脉高压性脾功能亢进患者腹腔镜脾切除术后并发门静脉系统血栓形成的危险因素研究. 中国普外基础与临床杂志, 2018, 25(4): 438-443.
18.	Jiang GQ, Bai DS, Chen P, et al. Risk factors for portal vein system thrombosis after laparoscopic splenectomy in cirrhotic patients with hypersplenism. J Laparoendosc Adv Surg Tech A, 2016, 26(6): 419-423.
19.	Webb NJ, Bottomley MJ, Watson CJ, et al. Vascular endothelial growth factor (VEGF) is released from platelets during blood clotting: implications for measurement of circulating VEGF levels in clinical disease. Clin Sci (Lond), 1998, 94(4): 395-404.
20.	庞兴学, 王显. 血栓形成的过程与机制研究进展. 医学综述, 2011, 17(11): 1613-1616.
21.	黄子健, 霍景山. 深静脉血栓形成早期试验指标的研究进展. 中国普通外科杂志, 2016, 25(6): 913-918.
22.	Uccelli A, Wolff T, Valente P, et al. Vascular endothelial growth factor biology for regenerative angiogenesis. Swiss Med Wkly, 2019, 149: w20011.

1. 刘军, 曲凯, 万永, 等. 门静脉压力无创测量方法在门静脉高压症诊疗中的应用. 中华普通外科杂志, 2013, 28(2): 164-166.
2. 中华外科学分会门静脉高压症学组. 肝硬化门静脉高压症食管胃底静脉曲张破裂出血的诊治共识 (2015 版). 中华普通外科杂志, 2016, 31(2): 167-170.
3. Kuroki T, Kitasato A, Tokunaga T, et al. Predictors of portal and splenic vein thrombosis after laparoscopic splenectomy: a retrospective analysis of a single-center experience. Surg today, 2018, 48(8): 804-809.
4. Hirashita T, Ohta M, Kai S, et al. Implications of portal vein thrombosis after splenectomy for patients with idiopathic portal hypertension. Surg Today, 2011, 41(11): 1475-1480.
5. 尚玉清, 朱虹霖. 门体断流术治疗门静脉高压症后门静脉系统血栓形成的危险因素分析. 中国普外基础与临床杂志, 2016, 23(12): 1488-1491.
6. Basit SA, Stone CD, Gish R. Portal vein thrombosis. Clin Liver Dis, 2015, 19(1): 199-221.
7. 戴朝六, 徐锋, 金添强. 重视门静脉高压症脾切除断流术后门静脉系统血栓的诊治. 中华普通外科学文献 (电子版), 2019, 13(3): 169-174.
8. Verheul HM, Jorna AS, Hoekman K, et al. Vascular endothelial growth factor-stimulated endothelial cells promote adhesion and activation of platelets. Blood, 2000, 96(13): 4216-4221.
9. Nosaka M, Ishida Y, Kimura A, et al. Absence of IFN-γ accelerates thrombus resolution through enhanced MMP-9 and VEGF expression in mice. J Clin Invest, 2011, 121(7): 2911-2920.
10. Vermeersch E, Denorme F, Maes W, et al. The role of platelet and endothelial GARP in thrombosis and hemostasis. PloS One, 2017, 12(3): e0173329.
11. Vogel S, Thein SL. Platelets at the crossroads of thrombosis, inflammation and haemolysis. Br J Haematol, 2018, 180(5): 761-767.
12. Shim JW, Madsen JR. VEGF signaling in neurological disorders. Int J Mol Sci, 2018, 19(1): 275.
13. Dalal ElSayed Abdelaal. Vascular endothelial growth factor in diagnosis of pleural effusion. Egypt J Chest Dis Tuberc, 2017, 66(1): 115-119.
14. Möhle R, Green D, Moore MA, et al. Constitutive production and thrombin-induced release of vascular endothelial growth factor by human megakaryocytes and platelets. Proc Natl Acad Sci U S A, 1997, 94(2): 663-668.
15. O’Byrne KJ, Dobbs N, Propper D, et al. Vascular endothelial growth factor platelet counts, and prognosis in renal cancer. Lancet, 1999, 353(9163): 1494-1495.
16. Kim SJ, Choi IK, Park KH, et al. Serum vascular endothelial growth factor per platelet count in hepatocellular carcinoma: correlations with clinical parameters and survival. Jpn J Clin Oncol, 2004, 34(4): 184-190.
17. 郑波, 廖东旭, 杨训, 等. 门静脉高压性脾功能亢进患者腹腔镜脾切除术后并发门静脉系统血栓形成的危险因素研究. 中国普外基础与临床杂志, 2018, 25(4): 438-443.
18. Jiang GQ, Bai DS, Chen P, et al. Risk factors for portal vein system thrombosis after laparoscopic splenectomy in cirrhotic patients with hypersplenism. J Laparoendosc Adv Surg Tech A, 2016, 26(6): 419-423.
19. Webb NJ, Bottomley MJ, Watson CJ, et al. Vascular endothelial growth factor (VEGF) is released from platelets during blood clotting: implications for measurement of circulating VEGF levels in clinical disease. Clin Sci (Lond), 1998, 94(4): 395-404.
20. 庞兴学, 王显. 血栓形成的过程与机制研究进展. 医学综述, 2011, 17(11): 1613-1616.
21. 黄子健, 霍景山. 深静脉血栓形成早期试验指标的研究进展. 中国普通外科杂志, 2016, 25(6): 913-918.
22. Uccelli A, Wolff T, Valente P, et al. Vascular endothelial growth factor biology for regenerative angiogenesis. Swiss Med Wkly, 2019, 149: w20011.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significance of the expression of VEGF in postoperative portal venous thrombosis in patients with portal hypertension

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