• Department of Colorectal Cancer Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, P. R. China;
WANG Guiyu, Email: guiywang@163.com
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Objective To summarize the common mouse models and the latest research progress in the basic research of colorectal cancer, introducing advantages, disadvantages and applications of these various models, provide references for the researchers in the selection of mouse models for their experiments.Method Retrieved the related literatures from databases including PubMed, Web of Science, CNKI and WanFang, and after reading the literatures, different methods were sorted out, analyzed and summarized.Results The mouse models commonly used in colorectal cancer research include the cell-derived xenograft (CDX), patient-derived xenograft (PDX), chemical reagent-induced tumor in situ, transgenic mice (ApcMin/+ mice), tumor cells derived from mice themselves were inoculated to the normal mice, and models of colorectal metastatic tumors (including liver, lung, abdomen and bone metastases, etc.). The CDX model cost shorter time to establish, and the PDX model restored the authentic phenotype of the tumor in patients, but the tumor were both colonized under the skin of nude mice, which lacked authenticity tumor microenvironment. The colorectal cancer in mice induced by chemical reagents and genetically engineered mice imitated the development of colorectal tumor in the situ intestine of mouse, but both of them were time-consuming. The model established by the tumor of mouse own was convenient for basic immune research of colorectal cancer, but the disadvantage was the unreal tumor microenvironment. The colorectal cancer metastasis model was an essential model for the study of the mechanism and treatment in metastasis colorectal tumor, but its establishment required higher operating skills and required the image examination to determine the whether the metastasis tumor was successfully generated or not.Conclusions Different mouse models of colorectal cancer have different emphases, advantages and disadvantages. Researchers need to make accurate selection according to the research purpose and design needs.

Citation: ZHANG Yukun, HU Hanqing, SHENG Xiangzong, WU Hongyu, ZHANG Qian, WANG Guiyu. Summary and comparison of mouse models in the basic research in colorectal cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2021, 28(6): 816-820. doi: 10.7507/1007-9424.202009011 Copy

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