Expression of contactin<b>-</b>1 in breast cancer tissues and its relation with prognosis of patients with breast cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HE Sai ¹ , TIAN Yanchao ² , GENG Jie ³ , GAO Hongbian ⁴ , CHEN Nan ¹ , HOU Yanni ¹ , FAN Yongguo ¹ ,  YANG Xiaomin ¹

1. Department of Breast Oncology, Shaanxi Provincial Cancer Hospital, Xi’an 710061, P. R. China;
2. Department of Burn and Plastic Surgery, The 964th Hospital of People’s Liberation Army, Changchun 130062, P. R. China;
3. Department of Cardiovascular Internal Medicine, Second Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710004, P. R. China;
4. Department of Pathology, Shaanxi Provincial Cancer Hospitall, Xi’an 710061, P. R. China;

Corresponding author：

YANG Xiaomin, Email: 10228373@qq.com

Keywords：

breast cancer; contactin-1 gene; prognosis

DOI：

10.7507/1007-9424.202108116

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To detect the expression of contactin-1 (CNTN1) gene in the breast cancer tissues and explore the relation between the expression and prognosis of patients with breast cancer. Methods The clinicopathologic data of 35 patients with breast cancer who met the inclusion criteria from January to June in 2015 in the Shaanxi Provincial Cancer Hospital were retrospectively collected. The Western blot and immunohistochemistry methods were used to detect the CNTN1 protein expressions in the 35 breast cancer tissues and their corresponding tissues adjacent to cancer and the reverse transcriptase-PCR method was used to detect the CNTN1 mRNA expression. The relation between the CNTN1 protein expression and 5-year overall survival (OS) was analyzed. Results The positive rate and expression level of CNTN1 protein in the breast cancer tissues were higher than those in the tissues adjacent to cancer [65.7% (23/35) vs. 45.7% (16/35), χ²=8.235, P=0.003; 0.825±0.221 vs. 0.412±0.117, t=12.556, P<0.001], and the expression level of CNTN1 mRNA in the breast cancer tissue was also higher than that in the corresponding tissues adjacent to cancer (0.763±0.218 vs. 0.353±0.135, t=11.162, P<0.001). The positive rates of CNTN1 protein were higher in the patients with larger tumor diameter (>2 cm), lower differentiation, later TNM stage (stage Ⅲ+Ⅳ) and axillary lymph node metastasis (P<0.05). The median OS of 35 patients with breast cancer was 47.3 months, which was 36.2 months and 52.5 months in the patients with high CNTN1 expression and low CNTN1 expression (median expression of CNTN1 protein was 0.785 as critical value) respectively (χ²=4.052, P=0.049). Conclusion Preliminary results of this study suggest that overexpression of CNTN1 gene might play an important role in occurrence and progression of primary breast cancer and is related to prognosis of survival.

Citation： HE Sai, TIAN Yanchao, GENG Jie, GAO Hongbian, CHEN Nan, HOU Yanni, FAN Yongguo, YANG Xiaomin. Expression of contactin-1 in breast cancer tissues and its relation with prognosis of patients with breast cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2022, 29(6): 726-730. doi: 10.7507/1007-9424.202108116 Copy

1.	李哲轩, 张阳, 周彤, 等. 2020全球癌症统计报告解读. 肿瘤综合治疗电子杂志, 2021, 7(2): 1-13.
2.	Hooper RC, Hsu J, Duncan A, et al. Breast cancer knowledge and decisions made for contralateral prophylactic mastectomy: a survey of surgeons and women in the general population. Plast Reconstr Surg, 2019, 143(5): 936e-945e. doi: 10.1097/PRS.0000000000005523.
3.	Lee J. A meta-analysis of the association between physical activity and breast cancer mortality. Cancer Nurs, 2019, 42(4): 271-285.
4.	Li ZX, Chong SY, Gulimiran A, et al. Effect of sulindac derivative K-80003 in combination with MEK inhibitor cobimetinib in breast cancer cells. Chinese Pharmacological Bulletin, 2019, 35(2): 251-254.
5.	Gu Y, Li T, Kapoor A, et al. Contactin 1: an important and emerging oncogenic protein promoting cancer progression and metastasis. Genes (Basel), 2020, 11(8): 874. doi: 10.3390/genes11080874.
6.	Cortese A, Lombardi R, Briani C, et al. Antibodies to neurofascin, contactin-1, and contactin-associated protein 1 in CIDP: clinical relevance of IgG isotype. Neurol Neuroimmunol Neuroinflamm, 2019, 7(1): e639. doi: 10.1212/NXI.0000000000000639.
7.	Liang Y, Ma C, Li F, et al. The role of contactin 1 in cancers: what we know so far. Front Oncol, 2020, 10: 574208. doi: 10.3389/fonc.2020.574208.
8.	王建君, 徐小艳, 冯智坤, 等. 乳腺癌中PTEN和Basigin1蛋白的表达与其临床病理学特征的关系. 中国普外基础与临床杂志, 2020, 27(7): 825-830.
9.	Span PN, Sweep FC, Wiegerinck ET, et al. Survivin is an independent prognostic marker for risk stratification of breast cancer patients. Clin Chem, 2004, 50(11): 1986-1993.
10.	曹毛毛, 陈万青. GLOBOCAN 2020全球癌症统计数据解读. 中国医学前沿杂志(电子版), 2021, 13(3): 63-69.
11.	Ramirez JM, Fehm T, Orsini M, et al. Prognostic relevance of viable circulating tumor cells detected by EPISPOT in metastatic breast cancer patients. Clin Chem, 2014, 60(1): 214-221.
12.	任云晓, 肖茹丹, 娄晓敏, 等. 基因编辑技术及其在基因治疗中的应用. 遗传, 2019, 41(1): 18-27.
13.	Srinivas PR, Srivastava S, Hanash S, et al. Proteomics in early detection of cancer. Clin Chem, 2001, 47(10): 1901-1911.
14.	贺赛, 耿洁, 杨晓民, 等. shRNR靶向沉默CNTN1表达对人乳腺癌细胞株MDA-MB-468裸鼠皮下移植瘤的抑制作用. 中国普外基础与临床杂志, 2020, 27(11): 1371-1374.
15.	Anderson C, Gerding WM, Fraenz C, et al. PLP1 and CNTN1 gene variation modulates the microstructure of human white matter in the corpus callosum. Brain Struct Funct, 2018, 223(8): 3875-3887.
16.	Berglund EO, Ranscht B. Molecular cloning and in situ localization of the human contactin gene (CNTN1) on chromosome 12q11-q12. Genomics, 1994, 21(3): 571-582.
17.	Illa I. ARTHUR ASBURY LECTURE: Chronic inflammatory demyelinating polyradiculoneuropathy: clinical aspects and new animal models of auto-immunity to nodal components. J Peripher Nerv Syst, 2017, 22(4): 418-424.
18.	Zhang X, Shi X, Zhao H, et al. Identification and validation of a tumor microenvironment-related gene signature for prognostic prediction in advanced-stage non-small-cell lung cancer. Biomed Res Int, 2021, 2021: 8864436. doi: 10.1155/2021/8864436.
19.	Su JL, Yang PC, Shih JY, et al. Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer Cell, 2006, 66(5): 2553-2561.
20.	Lamprianou S, Chatzopoulou E, Thomas JL, et al. A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells. Proc Natl Acad Sci USA, 2011, 108(42): 17498-17503.
21.	Yan J, Wong N, Hung C, et al. Contactin-1 reduces E-cadherin expression via activating AKT in lung cancer. PLoS One, 2013, 8(5): e65463. doi: 10.1371/journal.pone.0065463.
22.	Zhang D, Zhou S, Liu B. Identification and validation of an individualized EMT-related prognostic risk score formula in gastric adenocarcinoma patients. Biomed Res Int, 2020, 2020: 7082408. doi: 10.1155/2020/7082408.
23.	Chen DH, Yu JW, Jiang BJ. Contactin 1: a potential therapeutic target and biomarker in gastric cancer. World J Gastroenterol, 2015, 21(33): 9707-9716.
24.	Li G, Zhang Z, Ge G, et al. Upregulated CNTN1 is associated with lymph node metastasis and poor prognosis of colorectal cancer. Cancer Biomark, 2021, 30(2): 193-201.
25.	Wang B, Yang X, Zhao T, et al. Upregulation of contactin-1 expression promotes prostate cancer progression. Oncol Lett, 2020, 19(2): 1611-1618.
26.	Szász AM, Lánczky A, Nagy Á, et al. Cross-validation of survival associated biomarkers in gastric cancer using transcriptomic data of 1 065 patients. Oncotarget, 2016, 7(31): 49322-49333.
27.	Alkhathami AG, Verma AK, Alfaifi M, et al. Role of miRNA-495 and NRXN-1 and CNTN-1 mRNA expression and its prognostic importance in breast cancer patients. J Oncol, 2021, 2021: 9657071. doi: 10.1155/2021/9657071.

1. 李哲轩, 张阳, 周彤, 等. 2020全球癌症统计报告解读. 肿瘤综合治疗电子杂志, 2021, 7(2): 1-13.
2. Hooper RC, Hsu J, Duncan A, et al. Breast cancer knowledge and decisions made for contralateral prophylactic mastectomy: a survey of surgeons and women in the general population. Plast Reconstr Surg, 2019, 143(5): 936e-945e. doi: 10.1097/PRS.0000000000005523.
3. Lee J. A meta-analysis of the association between physical activity and breast cancer mortality. Cancer Nurs, 2019, 42(4): 271-285.
4. Li ZX, Chong SY, Gulimiran A, et al. Effect of sulindac derivative K-80003 in combination with MEK inhibitor cobimetinib in breast cancer cells. Chinese Pharmacological Bulletin, 2019, 35(2): 251-254.
5. Gu Y, Li T, Kapoor A, et al. Contactin 1: an important and emerging oncogenic protein promoting cancer progression and metastasis. Genes (Basel), 2020, 11(8): 874. doi: 10.3390/genes11080874.
6. Cortese A, Lombardi R, Briani C, et al. Antibodies to neurofascin, contactin-1, and contactin-associated protein 1 in CIDP: clinical relevance of IgG isotype. Neurol Neuroimmunol Neuroinflamm, 2019, 7(1): e639. doi: 10.1212/NXI.0000000000000639.
7. Liang Y, Ma C, Li F, et al. The role of contactin 1 in cancers: what we know so far. Front Oncol, 2020, 10: 574208. doi: 10.3389/fonc.2020.574208.
8. 王建君, 徐小艳, 冯智坤, 等. 乳腺癌中PTEN和Basigin1蛋白的表达与其临床病理学特征的关系. 中国普外基础与临床杂志, 2020, 27(7): 825-830.
9. Span PN, Sweep FC, Wiegerinck ET, et al. Survivin is an independent prognostic marker for risk stratification of breast cancer patients. Clin Chem, 2004, 50(11): 1986-1993.
10. 曹毛毛, 陈万青. GLOBOCAN 2020全球癌症统计数据解读. 中国医学前沿杂志(电子版), 2021, 13(3): 63-69.
11. Ramirez JM, Fehm T, Orsini M, et al. Prognostic relevance of viable circulating tumor cells detected by EPISPOT in metastatic breast cancer patients. Clin Chem, 2014, 60(1): 214-221.
12. 任云晓, 肖茹丹, 娄晓敏, 等. 基因编辑技术及其在基因治疗中的应用. 遗传, 2019, 41(1): 18-27.
13. Srinivas PR, Srivastava S, Hanash S, et al. Proteomics in early detection of cancer. Clin Chem, 2001, 47(10): 1901-1911.
14. 贺赛, 耿洁, 杨晓民, 等. shRNR靶向沉默CNTN1表达对人乳腺癌细胞株MDA-MB-468裸鼠皮下移植瘤的抑制作用. 中国普外基础与临床杂志, 2020, 27(11): 1371-1374.
15. Anderson C, Gerding WM, Fraenz C, et al. PLP1 and CNTN1 gene variation modulates the microstructure of human white matter in the corpus callosum. Brain Struct Funct, 2018, 223(8): 3875-3887.
16. Berglund EO, Ranscht B. Molecular cloning and in situ localization of the human contactin gene (CNTN1) on chromosome 12q11-q12. Genomics, 1994, 21(3): 571-582.
17. Illa I. ARTHUR ASBURY LECTURE: Chronic inflammatory demyelinating polyradiculoneuropathy: clinical aspects and new animal models of auto-immunity to nodal components. J Peripher Nerv Syst, 2017, 22(4): 418-424.
18. Zhang X, Shi X, Zhao H, et al. Identification and validation of a tumor microenvironment-related gene signature for prognostic prediction in advanced-stage non-small-cell lung cancer. Biomed Res Int, 2021, 2021: 8864436. doi: 10.1155/2021/8864436.
19. Su JL, Yang PC, Shih JY, et al. Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer Cell, 2006, 66(5): 2553-2561.
20. Lamprianou S, Chatzopoulou E, Thomas JL, et al. A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells. Proc Natl Acad Sci USA, 2011, 108(42): 17498-17503.
21. Yan J, Wong N, Hung C, et al. Contactin-1 reduces E-cadherin expression via activating AKT in lung cancer. PLoS One, 2013, 8(5): e65463. doi: 10.1371/journal.pone.0065463.
22. Zhang D, Zhou S, Liu B. Identification and validation of an individualized EMT-related prognostic risk score formula in gastric adenocarcinoma patients. Biomed Res Int, 2020, 2020: 7082408. doi: 10.1155/2020/7082408.
23. Chen DH, Yu JW, Jiang BJ. Contactin 1: a potential therapeutic target and biomarker in gastric cancer. World J Gastroenterol, 2015, 21(33): 9707-9716.
24. Li G, Zhang Z, Ge G, et al. Upregulated CNTN1 is associated with lymph node metastasis and poor prognosis of colorectal cancer. Cancer Biomark, 2021, 30(2): 193-201.
25. Wang B, Yang X, Zhao T, et al. Upregulation of contactin-1 expression promotes prostate cancer progression. Oncol Lett, 2020, 19(2): 1611-1618.
26. Szász AM, Lánczky A, Nagy Á, et al. Cross-validation of survival associated biomarkers in gastric cancer using transcriptomic data of 1 065 patients. Oncotarget, 2016, 7(31): 49322-49333.
27. Alkhathami AG, Verma AK, Alfaifi M, et al. Role of miRNA-495 and NRXN-1 and CNTN-1 mRNA expression and its prognostic importance in breast cancer patients. J Oncol, 2021, 2021: 9657071. doi: 10.1155/2021/9657071.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of contactin-1 in breast cancer tissues and its relation with prognosis of patients with breast cancer

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content