Objective To investigate the effects of liver X receptor agonist, T0901317, on the proliferation, migration and hydroxyproline production of human embryonic lung fibroblasts (HELF). Methods HELF cells were devided into a control group, a growth factor group, a T0901317 group and three growth factor+T0901317 groups. The cells of the control group were treated with Dulbecco's modified Eagle medium. The cells of T0901317 group were treated with 1.00 μmol/L T0901317. The growth factor+T0901317 groups were incubated with different doses of T0901317 (0.25 μmol/L, 0.50 μmol/L and 1.00 μmol/L) for 2 h. Then the cells of the growth factor+T0901317 groups and the growth factor group were incubated with basic fibroblast growth factor and transforming growth factor-β1 for 24 h. The proliferation, migration and collagen production of HELF were determined by cell counting kit-8 method, transwell chamber, and hydroxyproline method. Results Compared with the control group, T0901317 had no effect on the proliferation, migration and hydroxyproline production of HELF. Growth factors could promote the proliferation, migration and hydroxyproline production of HELF significantly. T0901317 could inhibit those effects of growth factors with a dosage-dependent manner. Conclusion T0901317 may inhibit the proliferation, migration and hydroxyproline production of HELF induced by growth factors.

Citation： ShiYing, TanYan, GuWei. Effects of Liver X Receptor Agonist, T0901317, on the Proliferation, Migration and Hydroxyproline Production of Human Embryonic Lung Fibroblasts. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(2): 143-146. doi: 10.7507/1671-6205.2015037 Copy