The Effect of Medical Ozone on Lung Tissue of Pulmonary Fibrosis Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZhaoShunxin ,  LiuJianbo

Department of Respiratory Medicine, The Second Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, 450000, China;

Corresponding author：

LiuJianbo, Email: jbliuzz@163.com

Keywords：

Pulmonary fibrosis; Medical ozone; Superoxide dismutase; Hydroxyproline

DOI：

10.7507/1671-6205.2016010

Video：

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Objective To explore the feasibility of medical ozone in treatment of pulmonary fibrosis. Methods Forty Wistar rats were randomly divided into an experimental group and a control group, with 20 rats in each group.All rats were intratreacheally instilled with bleomycin to induce pulmonary fibrosis.Then the rats were intraperitoneally injected with physiological saline every other day in the control group, and with medical ozone every other day in the experimental group.After 28 days, 10 rats in each group were sacrificed after lung function test.Right lung tissues were sampled for pathological examination, and left lung tissues were sampled for measurement of superoxide dismutase (SOD) and hydroxyproline.The remaining 10 rats in each group continued to be normally fed and intraperitoneally injected for observation of the survival time. Results The lung function of the control group significantly decreased compared with the experimental group.The degree of lung fibrosis in the control group was more severe than that in the experimental group (lung fibrosis score: 1.9±0.5 vs.1.2±0.4, P < 0.05). The level of SOD in lung tissue was significantly higher and the level of hydroxyproline was significantly lower in the experimental group compared with the control group [(208.48±29.37)U·mg^-1·pro^-1 vs.(163.34±21.42) U·mg^-1·pro^-1, (2.25±0.28) mg/g vs.(2.68±0.37) mg/g, P < 0.05].The rats in the experimental group had longer survival time compared with the control group (79 d vs.59 d, P < 0.05). Conclusion Medical ozone can delay the progress of pulmonary fibrosis in rats.

Citation： ZhaoShunxin, LiuJianbo. The Effect of Medical Ozone on Lung Tissue of Pulmonary Fibrosis Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(1): 39-42. doi: 10.7507/1671-6205.2016010 Copy

1.	葛均波, 徐永健, 等.内科学.第8版.北京:人民卫生出版社, 2013, 89-92.
2.	蔡后荣.2011年特发性肺纤维化诊断和治疗循证新指南解读.中国呼吸与危重监护杂志, 2011, 10:313-316.
3.	黄霞, 刘惠霞, 孙为, 等.不同治则对肺间质纤维化大鼠氧自由基损伤的干预作用.中国实验方剂学杂志, 2010, 16:188-191.
4.	Tanjore H, Blackwell TS, Lawson WE. Emerging evidence for endoplasmic reticulum stress in the pathogenesis of idiopathic pulmonary fibrosis.Am J Physiol Lung Cell Mol Physiol, 2012, 302:L721-L729.
5.	Peralta C, Bulbena O, Xaus C.Ischemic preconditioning:a defense mechanism against the reactive oxygen species generated after hepatic ischemia reperfusion. Transplantation, 2002, 73:1203-1207.
6.	Hagiwara SI, Ishii Y, Kitamura S.Aerosolized administration of N-acetylcysteine allenuates lung fibrosis induced by bleomycin in mice. Am J Respir Crit Care Med, 2000, 162:225-230.
7.	Zeng M, Liao B, Zhu C, et al.Aerosolized STAT1 antisense oligodeoxynucleotides decrease the concentrations of inflammatory mediators in bronchoalveolar lavage fluid in bleomycin-induced rat pulmonary fibrosis.Cell Mol Immunol, 2008:219-224.
8.	Long X, Xiong SD, Xiong WN, et al. Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bleomycin-induced lung fibrosis in rats.Clin Med J(Engl), 2007, 120:1432-1437.
9.	陈卫巧, 杜国平.医用臭氧安全性的研究进展.中国医学创新, 2015, 12:147-149.
10.	Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin-induced interstitial pulmonary disease in the nude, athymic mouse.Am Rev Respir Dis, 1979, 120:893-899.
11.	李惠萍, 王思勤, 周建英, 等.国产吡非尼酮治疗特发性肺纤维化Ⅱ期临床研究.中国呼吸与危重监护杂志, 2015, 14:229-235.
12.	Oruqaj G, Karnati S, Vijayan V, et al.Compromised peroxisomes in idiopathic pulmonary fibrosis, a vicious cycle inducing a higher fibrotic response via TGF-βsignaling.Proc Natl Acad Sci USA, 2015, 112:E2048-E2057.
13.	Fattman CL, Schaefer LM, Oury TD.Extracellular superoxide dismutase in biology and medicine.Free Radic Biol Med, 2003, 35:236-256.
14.	Cooper AD. Pulmonary fibrosis:pathways are slowly coming into light.Am J Respir Cell Mol Biol, 2000, 22:520-523.
15.	Larini A, Bianchi L, Bocci V. The ozone to leranc:enhancement of antioxidant enzymes is ozone dose dependent in Jurkat cells.Free Radic Res, 2003, 37:1163-1168.
16.	Russell PB, James DC. Oxidative stress in airways:is there a role for extracellular superoxide dismutase.Am J Respir Crit Care Med, 2002, 166:38-43.
17.	邱勤业, 余斌.医用臭氧对金黄色葡萄球菌和大肠埃希菌的杀灭作用.南方医科大学学报, 2010, 30:929-930.

1. 葛均波, 徐永健, 等.内科学.第8版.北京:人民卫生出版社, 2013, 89-92.
2. 蔡后荣.2011年特发性肺纤维化诊断和治疗循证新指南解读.中国呼吸与危重监护杂志, 2011, 10:313-316.
3. 黄霞, 刘惠霞, 孙为, 等.不同治则对肺间质纤维化大鼠氧自由基损伤的干预作用.中国实验方剂学杂志, 2010, 16:188-191.
4. Tanjore H, Blackwell TS, Lawson WE. Emerging evidence for endoplasmic reticulum stress in the pathogenesis of idiopathic pulmonary fibrosis.Am J Physiol Lung Cell Mol Physiol, 2012, 302:L721-L729.
5. Peralta C, Bulbena O, Xaus C.Ischemic preconditioning:a defense mechanism against the reactive oxygen species generated after hepatic ischemia reperfusion. Transplantation, 2002, 73:1203-1207.
6. Hagiwara SI, Ishii Y, Kitamura S.Aerosolized administration of N-acetylcysteine allenuates lung fibrosis induced by bleomycin in mice. Am J Respir Crit Care Med, 2000, 162:225-230.
7. Zeng M, Liao B, Zhu C, et al.Aerosolized STAT1 antisense oligodeoxynucleotides decrease the concentrations of inflammatory mediators in bronchoalveolar lavage fluid in bleomycin-induced rat pulmonary fibrosis.Cell Mol Immunol, 2008:219-224.
8. Long X, Xiong SD, Xiong WN, et al. Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bleomycin-induced lung fibrosis in rats.Clin Med J(Engl), 2007, 120:1432-1437.
9. 陈卫巧, 杜国平.医用臭氧安全性的研究进展.中国医学创新, 2015, 12:147-149.
10. Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin-induced interstitial pulmonary disease in the nude, athymic mouse.Am Rev Respir Dis, 1979, 120:893-899.
11. 李惠萍, 王思勤, 周建英, 等.国产吡非尼酮治疗特发性肺纤维化Ⅱ期临床研究.中国呼吸与危重监护杂志, 2015, 14:229-235.
12. Oruqaj G, Karnati S, Vijayan V, et al.Compromised peroxisomes in idiopathic pulmonary fibrosis, a vicious cycle inducing a higher fibrotic response via TGF-βsignaling.Proc Natl Acad Sci USA, 2015, 112:E2048-E2057.
13. Fattman CL, Schaefer LM, Oury TD.Extracellular superoxide dismutase in biology and medicine.Free Radic Biol Med, 2003, 35:236-256.
14. Cooper AD. Pulmonary fibrosis:pathways are slowly coming into light.Am J Respir Cell Mol Biol, 2000, 22:520-523.
15. Larini A, Bianchi L, Bocci V. The ozone to leranc:enhancement of antioxidant enzymes is ozone dose dependent in Jurkat cells.Free Radic Res, 2003, 37:1163-1168.
16. Russell PB, James DC. Oxidative stress in airways:is there a role for extracellular superoxide dismutase.Am J Respir Crit Care Med, 2002, 166:38-43.
17. 邱勤业, 余斌.医用臭氧对金黄色葡萄球菌和大肠埃希菌的杀灭作用.南方医科大学学报, 2010, 30:929-930.

Chinese Journal of Respiratory and Critical Care Medicine

The Effect of Medical Ozone on Lung Tissue of Pulmonary Fibrosis Rats

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