Objective
To investigate the role of macrophage stimulating protein (MSP) in the pathogenesis of chronic obstructive pulmonary disease (COPD).
Methods
Ninety subjects were recruited from health examination center, outpatient or inpatient department in Affiliated Hospital of North Sichuan Medical College from July 2013 to December 2013. They were divided intoahealthy control group, a stable COPD group, and an acute exacerbation of COPD (AECOPD) group with 30 subjects in each group. The levels of MSP, interleukin-6 (IL-6), IL-8 and tumor necrosis factor-α (TNF-α) in the plasma of all subjects, as well as the levels of MSP in the induced sputum of the AECOPD and the stable COPD patients were assessed by enzyme-linked-immuno-sorbent assay.
Results
The concentrations of MSP, IL-6, IL-8 and TNF-α in the plasma of the patients with COPD were obviously higher than those of the healthy controls (P<0.05) while much higher in AECOPD patients than those in stable COPD patients (P<0.01).The concentration of MSP in the induced sputum of the patients with AECOPD was higher than that in the stable COPD patients (P<0.01). The concentrations of MSP in the serum and induced sputum as well as serum levels of IL-6, IL-8 and TNF-α in the patients with COPD were negatively correlated with the level of FEV1%pred. The concentrations of MSP in the serum and induced sputum in the COPD patients were positively correlated with the concentrations of IL-6, IL-8 and TNF-α.
Conclusions
The concentrations of serum and induced sputum MSP, and the serum concentrations of IL-6, IL-8 and TNF-α in COPD patients are related to the severity of the disease. MSP may play an important role in the pathogenesis of COPD. The mechanism might be mainly involved in the regulation of airway inflammation.
Citation：
ZhangWenbo, ChenXiaoju, LengChangyan, XiangXiaojun. The Expression and Significance of Macrophage Stimulating Protein in Patients with Chronic Obstructive Pulmonary Disease. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(2): 115-118. doi: 10.7507/1671-6205.2016029
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Copyright © the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
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陈小菊, 王涛, 陈心容, 等.MSP对COPD大鼠肺泡巨噬细胞氧化应激和细胞因子产生的影响.求医问药, 2011, 9:172-173.
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Zhao J, Hu L, Gong N, et al.The effects of macrophage-stimulating protein on the migration, proliferation, and collagen synthesis of skin fibroblasts in vitro and in vivo.Tissue Eng Part A, 2015, 21:982-991.
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- 1. Chung KF.Inflammatory mediators in chronic obstructive pulmonary disease.Curr Drug Targets Inflamm Allergy, 2005, 4:619-625.
- 2. Sharafkhaneh A, Hanania NA, Kim V.Pathogenesis of emphysema:from the bench to the bedside.Proc Am Thorac Soc, 2008, 5:475-477.
- 3. Han S, Stuart LA, Degen SJ.Characterization of the DNF15S2 locus on human chromosome 3:identification ofagene coding for four kringle domains with homology to hepatocyte growth factor.Biochemistry, 1991, 30:9768-9780.
- 4. Wang MH, Skeel A, Leonard EJ.Proteolytic cleavage and activation of pro-macrophage-stimulating protein by resident peritoneal macrophage membrane proteases.J Clin Invest, 1996, 97:720-727.
- 5. Wang MH, Zhou YQ, Chen YQ.Macrophage-stimulating protein and RON receptor tyrosine kinase:potential regulators of macrophage inflammatory activities.Scand J Immunol, 2002, 56:545-553.
- 6. Cowin AJ, Kallincos N, Hatzirodos N, et al.Hepatocyte growth factor and macrophage-stimulating protein are upregulated during excisional wound repair in rats.Cell Tissue Res, 2001, 306:239-250.
- 7. 陈小菊, 王涛, 陈心容, 等.MSP对COPD大鼠肺泡巨噬细胞氧化应激和细胞因子产生的影响.求医问药, 2011, 9:172-173.
- 8. 卫生部司政司.慢性阻塞性肺疾病诊疗规范(2011年版).中国医学前沿杂志(电子版), 2012, (1):69-76.
- 9. Bhatt AS, Welm A, Farady CJ, et al.Coordinate expression and functional profiling identify an extracellular proteolytic signaling pathway.Proc Natl Acad Sci USA, 2007, 104:5771-5776.
- 10. Bezerra JA, Carrick TL, Degen JL, et al.Biological effects of targeted inactivation of hepatocyte growth factor-like protein in mice.J Clin Invest, 1998, 101:1175-1183.
- 11. Zhao J, Hu L, Gong N, et al.The effects of macrophage-stimulating protein on the migration, proliferation, and collagen synthesis of skin fibroblasts in vitro and in vivo.Tissue Eng Part A, 2015, 21:982-991.
