Expression of Long Non-Coding RNA KCNQ1OT1 and Its Clinical Significance in Non-Small Cell Lung Cancer Tissues_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LiJing ¹ , LiuXiaoqin ² , YangYe ² ,  LiuBin ²

1. The Second Outpatient Department, Sichuan Cancer Hospital, Chengdu, Sichuan, 610041, China;
2. ;

Corresponding author：

LiuBin, Email: binliu322@126.com

Keywords：

LncRNA KCNQ1OT1; Non-small cell lung cancer; Prognosis

DOI：

10.7507/1671-6205.2016106

Video：

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Objective To explore the expression of long non-coding RNA (LncRNA) KCNQ1OT1 and its clinical significance in non-small cell lung cancer (NSCLC). Methods Eighty-nine NSCLC patients who underwent surgery were recruited in Sichuan Cancer Hospital from January 2011 to December 2013. Quantitative real-time PCR was used to detect the expression of LncRNA KCNQ1OT1 in tumor tissues and paracarcinoma tissues (5cm or above away from tumor). The relationship between LncRNA KCNQ1OT1 expression and clinicopathologic features was analyzed by univariate analysis and Cox regression analysis. Results The expression of LncRNA KCNQ1OT1 significantly increased in tumor tissues than that in paracarcinoma tissues (P < 001). The patients were divided into a high expression group and a low expression group according to the relative expression of LncRNA KCNQ1OT1. Univariate analysis showed that the differences between two groups were not significant in age, gender or histological type, but were significant in tumor size (χ²=12.619, P < 001), lymph node metastasis(χ²=10.298, P=0.001), TNM stage(χ²=7.199, P=0.007), and history of smoking(χ²=24.005, P < 001). Kaplan-Meier analysis showed the patients with high LncRNA KCNQ1OT1 expression had significantly lower overall survival time (20.0 months vs. 35.0 months, χ²=45.860, P < 001) and significantly lower progression-free survival time (12.0 months vs. 24.0 months, χ²=31.510, P < 001) than those with low LncRNA KCNQ1OT1 expression. Cox regression analysis revealed that the disease stage and the expression of LncRNA KCNQ1OT1 could be used as independent prognostic markers for poor prognosis. Conclusion LncRNA KCNQ1OT1 is highly expressed in tumor tissues and associated with the prognosis of NSCLC patients, thus can be used as a potential marker for predicting the prognosis of lung cancer.

Citation： Li Jing, Liu Xiaoqin, Yang Ye, Liu Bin. Expression of Long Non-Coding RNA KCNQ1OT1 and Its Clinical Significance in Non-Small Cell Lung Cancer Tissues. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(5): 453-457. doi: 10.7507/1671-6205.2016106 Copy

1.	Gong B, Jiang N, Yan G, et al.Predictors for severe acute esophagitis in lung cancer patients treated with chemoradiotherapy:a systematic review.Curr Med Res Opin, 2016:1-8.
2.	Turner RC, Lucke-Wold BP, Hwang R, et al.Lung cancer metastasis presenting as a solitary skull mass.J Surg Case Rep, 2016, 2016.pii:rjw116.
3.	Hong HH, Hou LK, Pan X, et al.Long non-coding RNA UCA1 is a predictive biomarker of cancer.Oncotarget, 2016[Epub ahead of print].
4.	Tu Z, He D, Deng X, et al.An eight-long non-coding RNA signature as a candidate prognostic biomarker for lung cancer.Oncol Rep, 2016, 36:215-222.
5.	Wang L, Chen Z, An L, et al.Analysis of long non-coding RNA expression profiles in non-small cell lung cancer.Cell Physiol Biochem, 2016, 38:2389-2400.
6.	Wang Y, Xue D, Li Y, et al.The long noncoding RNA MALAT-1 is a novel biomarker in various cancers:a meta-analysis based on the GEO database and literature.J Cancer, 2016, 7:991-1001.
7.	Zhou Y, Xu X, Lv H, et al.The long noncoding RNA MALAT-1 is highly expressed in ovarian cancer and induces cell growth and migration.PLoS One, 2016, 11:e0155250.
8.	Dou J, Ni Y, He X, et al.Decreasing lncRNA HOTAIR expression inhibits human colorectal cancer stem cells.Am J Transl Res, 2016, 8:98-108.
9.	Wang J, Chen D, He X, et al.Downregulated lincRNA HOTAIR expression in ovarian cancer stem cells decreases its tumorgeniesis and metastasis by inhibiting epithelial-mesenchymal transition.Cancer Cell Int, 2015, 15:24.
10.	Basile V, Baruffaldi F, Dolfini D, et al.NF-YA splice variants have different roles on muscle differentiation.Biochim Biophys Acta, 2016, 1859:627-638.
11.	Yao J, Zhao X, Ding X.Systematic profiling of chemotherapeutic drug response to EGFR gatekeeper mutation in non-small cell lung cancer.Comput Biol Chem, 2016, 64:126-133.
12.	Lee M, Kim HJ, Kim SW, et al.The long non-coding RNA HOTAIR increases tumour growth and invasion in cervical cancer by targeting the Notch pathway.Oncotarget, 2016.[Epub ahead of print].
13.	Luo ZF, Zhao D, Li XQ, et al.Clinical significance of HOTAIR expression in colon cancer.World J Gastroenterol, 2016, 22:5254-5259.
14.	Yang L, Zhang X, Li H, et al.The long noncoding RNA HOTAIR activates autophagy by upregulating ATG3 and ATG7 in hepatocellular carcinoma.Mol Biosyst, 2016.[Epub ahead of print].
15.	Guo F, Guo L, Li Y, et al.MALAT1 is an oncogenic long non-coding RNA associated with tumor invasion in non-small cell lung cancer regulated by DNA methylation.Int J Clin Exp Pathol, 2015, 8:15903-15910.
16.	Zhang Y, Wang T, Huang H.Q, et al.Human MALAT-1 long non-coding RNA is overexpressed in cervical cancer metastasis and promotes cell proliferation, invasion and migration.J BUON, 2015, 20:1497-1503.
17.	Liu XH, Liu ZL, Sun M, et al.The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer.BMC Cancer, 2013, 13:464.
18.	Qiu M, Xu Y, Yang X, et al.CCAT2 is a lung adenocarcinoma-specific long non-coding RNA and promotes invasion of non-small cell lung cancer.Tumour Biol, 2014, 35:5375-5380.
19.	Shi X, Sun M, Liu H, et al.A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer.Mol Carcinog, 2015, 54 Suppl 1:E1-e12.
20.	Han L, Kong R, Yin DD, et al.Low expression of long noncoding RNA GAS6-AS1 predicts a poor prognosis in patients with NSCLC.Med Oncol, 2013, 30:694.
21.	Sunamura N, Ohira T, Kataoka M, et al.Regulation of functional KCNQ1OT1 lncRNA by β-catenin.Sci Rep, 2016, 6:20690.
22.	Kanduri C.Long noncoding RNAs:lessons from genomic imprinting.Biochim Biophys Acta, 2016, 1859:102-111.
23.	Zhang Z, Weaver D.L, Olsen D, et al.Long non-coding RNA chromogenic in situ hybridisation signal pattern correlation with breast tumour pathology.J Clin Pathol, 2016, 69:76-81.
24.	Wan J, Huang M, Zhao H, et al.A novel tetranucleotide repeat polymorphism within KCNQ1OT1 confers risk for hepatocellular carcinoma.DNA Cell Biol, 2013, 32:628-634.

1. Gong B, Jiang N, Yan G, et al.Predictors for severe acute esophagitis in lung cancer patients treated with chemoradiotherapy:a systematic review.Curr Med Res Opin, 2016:1-8.
2. Turner RC, Lucke-Wold BP, Hwang R, et al.Lung cancer metastasis presenting as a solitary skull mass.J Surg Case Rep, 2016, 2016.pii:rjw116.
3. Hong HH, Hou LK, Pan X, et al.Long non-coding RNA UCA1 is a predictive biomarker of cancer.Oncotarget, 2016[Epub ahead of print].
4. Tu Z, He D, Deng X, et al.An eight-long non-coding RNA signature as a candidate prognostic biomarker for lung cancer.Oncol Rep, 2016, 36:215-222.
5. Wang L, Chen Z, An L, et al.Analysis of long non-coding RNA expression profiles in non-small cell lung cancer.Cell Physiol Biochem, 2016, 38:2389-2400.
6. Wang Y, Xue D, Li Y, et al.The long noncoding RNA MALAT-1 is a novel biomarker in various cancers:a meta-analysis based on the GEO database and literature.J Cancer, 2016, 7:991-1001.
7. Zhou Y, Xu X, Lv H, et al.The long noncoding RNA MALAT-1 is highly expressed in ovarian cancer and induces cell growth and migration.PLoS One, 2016, 11:e0155250.
8. Dou J, Ni Y, He X, et al.Decreasing lncRNA HOTAIR expression inhibits human colorectal cancer stem cells.Am J Transl Res, 2016, 8:98-108.
9. Wang J, Chen D, He X, et al.Downregulated lincRNA HOTAIR expression in ovarian cancer stem cells decreases its tumorgeniesis and metastasis by inhibiting epithelial-mesenchymal transition.Cancer Cell Int, 2015, 15:24.
10. Basile V, Baruffaldi F, Dolfini D, et al.NF-YA splice variants have different roles on muscle differentiation.Biochim Biophys Acta, 2016, 1859:627-638.
11. Yao J, Zhao X, Ding X.Systematic profiling of chemotherapeutic drug response to EGFR gatekeeper mutation in non-small cell lung cancer.Comput Biol Chem, 2016, 64:126-133.
12. Lee M, Kim HJ, Kim SW, et al.The long non-coding RNA HOTAIR increases tumour growth and invasion in cervical cancer by targeting the Notch pathway.Oncotarget, 2016.[Epub ahead of print].
13. Luo ZF, Zhao D, Li XQ, et al.Clinical significance of HOTAIR expression in colon cancer.World J Gastroenterol, 2016, 22:5254-5259.
14. Yang L, Zhang X, Li H, et al.The long noncoding RNA HOTAIR activates autophagy by upregulating ATG3 and ATG7 in hepatocellular carcinoma.Mol Biosyst, 2016.[Epub ahead of print].
15. Guo F, Guo L, Li Y, et al.MALAT1 is an oncogenic long non-coding RNA associated with tumor invasion in non-small cell lung cancer regulated by DNA methylation.Int J Clin Exp Pathol, 2015, 8:15903-15910.
16. Zhang Y, Wang T, Huang H.Q, et al.Human MALAT-1 long non-coding RNA is overexpressed in cervical cancer metastasis and promotes cell proliferation, invasion and migration.J BUON, 2015, 20:1497-1503.
17. Liu XH, Liu ZL, Sun M, et al.The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer.BMC Cancer, 2013, 13:464.
18. Qiu M, Xu Y, Yang X, et al.CCAT2 is a lung adenocarcinoma-specific long non-coding RNA and promotes invasion of non-small cell lung cancer.Tumour Biol, 2014, 35:5375-5380.
19. Shi X, Sun M, Liu H, et al.A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer.Mol Carcinog, 2015, 54 Suppl 1:E1-e12.
20. Han L, Kong R, Yin DD, et al.Low expression of long noncoding RNA GAS6-AS1 predicts a poor prognosis in patients with NSCLC.Med Oncol, 2013, 30:694.
21. Sunamura N, Ohira T, Kataoka M, et al.Regulation of functional KCNQ1OT1 lncRNA by β-catenin.Sci Rep, 2016, 6:20690.
22. Kanduri C.Long noncoding RNAs:lessons from genomic imprinting.Biochim Biophys Acta, 2016, 1859:102-111.
23. Zhang Z, Weaver D.L, Olsen D, et al.Long non-coding RNA chromogenic in situ hybridisation signal pattern correlation with breast tumour pathology.J Clin Pathol, 2016, 69:76-81.
24. Wan J, Huang M, Zhao H, et al.A novel tetranucleotide repeat polymorphism within KCNQ1OT1 confers risk for hepatocellular carcinoma.DNA Cell Biol, 2013, 32:628-634.

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Expression of Long Non-Coding RNA KCNQ1OT1 and Its Clinical Significance in Non-Small Cell Lung Cancer Tissues

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