The role and significance of interleukin-37 and related factors in the model of acute lung injury of rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 SUN Yunhui , WANG Yixin , MA Xuemei , YANG Xiaodong , BAO Wenhua

Department of Respiratory Medicine, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, P. R. China;

Corresponding author：

SUN Yunhui, Email: 394849603@qq.com

Keywords：

Acute lung injury; Interleukin-37; Interleukin-18; Tumor necrosis factor α

DOI：

10.7507/1671-6205.201705055

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To study the expression of interleukin (IL)-37 and related factors in lung tissue of rats with acute lung injury, and explore the role and significance of IL-37 in acute lung injury so as to understand the pathogenesis of acute lung injury. Methods Forty-five clean-grade Wistar rats were randomly divided into a healthy control group, a bleomycin group and a dexamethasone treatment group, with 15 rats in each group. The rats in the bleomycin group and the dexamethasone group were treated with bleomycin at a dose of 4 mg/kg by intratracheal administration. The healthy control group was given the same volume of saline as control. The dexamethasone treatment group was injected with dexamethasone 3 mg/kg intraperitoneally twice a day on the basis of acute lung injury. The healthy control group and the bleomycin group were injected intraperitoneally with normal saline as control. The rats in each group were sacrificed at 7, 14 and 28 days after modeling. The histopathological changes of lung tissue were evaluated by HE staining. The levels of IL-37 in lung homogenate and tumor necrosis factor (TNF)-α in serum were measured. The expression of IL-18 mRNA in lung tissue was detected by RT-PCR. Results Pathological morphology showed that the lung tissue of the healthy control group was complete, no inflammatory and fibrotic changes at all time points. The lung tissues of the bleomycin group and the dexamethasone group manifested with acute alveolitis firstly and thus developed fibrosis changes lately, and the changes in the dexamethasone group were more slightly than those in the bleomycin group. The contents of IL-37 and TNF-α and the expression of IL-18 mRNA in the bleomycin group and the dexamethasone group reached to the highest point on the 7^th day, and then decreased, but were significantly higher than those of the healthy control group on the 28^th day (all P<0.05). Conclusions IL-37 plays an important role in the pathogenesis of acute lung injury in rats. This effect may be related to the regulation of IL-18 and TNF-α transduction.

Citation： SUN Yunhui, WANG Yixin, MA Xuemei, YANG Xiaodong, BAO Wenhua. The role and significance of interleukin-37 and related factors in the model of acute lung injury of rats. Chinese Journal of Respiratory and Critical Care Medicine, 2017, 16(6): 586-590. doi: 10.7507/1671-6205.201705055 Copy

1.	顾晓凌, 宋勇. NALP3炎性体在急性肺损伤中的研究进展. 中国呼吸与危重监护杂志, 2012, 11(6): 607-609.
2.	Ganter MT, Roux J, Miyazawa B, et al. Interleukin- 1β causes acute lung injury via αvβ5 and αvβ6 integrin-dependent mechanisms. Circ Res, 2008, 102: 804-812.
3.	花扣珍, 陈建忠. 白细胞介素37在感染性疾病中的作用. 细胞与分子免疫学杂志, 2016, 32(3): 402-405.
4.	Degryse AL, Lawson WE. Progress toward improving animal models for idiopathic pulmonary fibrosis. Am J Med Sci, 2011, 341(6): 444-449.
5.	Kumar S, Mc Donnell PC, Lehr R, et al. Identification and initial characterization of four novel members of the interleukin-1 family. J Biol Chem, 2000, 275(14): 10308-10314.
6.	Dunn E, Sims JE, Nicklin MJ, et al. Annotating genes with potential roles in the immune system: six new members of the IL-1 family. Trends Immunol, 2001, 22(10): 533-536.
7.	袁立燕, 谢杰, 薛汝增, 等. 白细胞介素37的免疫学研究. 皮肤性病诊疗学杂志, 2016, 23(4): 280-284.
8.	Okamura H, Tsutsi H, Komatsu T, et al. Cloning of a new cytokine that induces IFN-γ production by T cells. Nature, 1995, 378(6552): 88.
9.	Biet F, Locht C, Kremer L. Immunoregulatory functions of interleukin 18 and its role in defense against bacterial pathogens. J Mol Med, 2002, 80(3): 147.
10.	Nold MF, Nold-Petry CA, Zepp JA, et al. IL-37 is a fundamental inhibitor of innate immunity. Nat Immunol, 2010, 11(11): 1014-1022.
11.	Sakai N, Van Sweringen HL, Belizaire RM, et al. Interleukin-37 reduces liver inflammatory injury via effects on hepatocytes and non-parenchymal cells. J Gastroenterol Hepatol, 2012, 27: 1609-1616.
12.	Boraschi D, Lucchesi D, Hainzl S, et al. IL-37: a new anti-inflammatory cytokine of the IL-1 family. Eur Cytokine Netw, 2011, 22(3): 127-147.
13.	许桂芳, 彭勇, 陈勇. 痛风患者血清中IL-37、IL-1β及IL-18水平检测及其相关性研究. 温州医科大学学报, 2014, 44(5): 370-373.
14.	Zhao PW, Jiang WG, Wang L, et al. Plasma levels of IL-37 and correlation with TNF-α, IL-17A, and disease activity during DMARD treatment of rheumatoid arthritis. PLoS One, 2014, 9(5): e95346.
15.	Imaeda H, Takahashi K, Fujimoto T, et al. Epithelial expression of interleukin-37b in inflammatory bowel disease. Clin Exp Immunol, 2013, 172: 410-416.
16.	Bufler P, Gamboni-Robertson F, Azam T, et al. Interleukin-1 homologues IL-1F7b and IL-18 contain functional mRNA instability elements within the coding region responsive to lipopolysaccharide. Biochem J, 2004, 381(Pt 2): 503-510.
17.	Hayashi N, Yoshimoto T, Izuhara K, et al. T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-gamma and IL-13 production. Proc Natl Acad Sci USA, 2007, 104(37): 14765-14770.
18.	吕盛秋, 李超乾. 白细胞介素-18在支气管哮喘中的研究进展. 免疫学杂志, 2014, 30(4): 347-350.
19.	Li HP, Li X, He GJ, et al. The influence of dexamethasone on the proliferation and apoptosis of pulmonary inflammatory cells in bleomycin-induced pulmonary fibrosis in rats. Respirology, 2004, 9: 25-32.
20.	Chen F, Gong L, Zhang L, et al. Short courses of low dose dexamethasone delay bleomycin-induced lung fibrosis in rats. Eur J Pharmacol, 2006, 536: 287-295.

1. 顾晓凌, 宋勇. NALP3炎性体在急性肺损伤中的研究进展. 中国呼吸与危重监护杂志, 2012, 11(6): 607-609.
2. Ganter MT, Roux J, Miyazawa B, et al. Interleukin- 1β causes acute lung injury via αvβ5 and αvβ6 integrin-dependent mechanisms. Circ Res, 2008, 102: 804-812.
3. 花扣珍, 陈建忠. 白细胞介素37在感染性疾病中的作用. 细胞与分子免疫学杂志, 2016, 32(3): 402-405.
4. Degryse AL, Lawson WE. Progress toward improving animal models for idiopathic pulmonary fibrosis. Am J Med Sci, 2011, 341(6): 444-449.
5. Kumar S, Mc Donnell PC, Lehr R, et al. Identification and initial characterization of four novel members of the interleukin-1 family. J Biol Chem, 2000, 275(14): 10308-10314.
6. Dunn E, Sims JE, Nicklin MJ, et al. Annotating genes with potential roles in the immune system: six new members of the IL-1 family. Trends Immunol, 2001, 22(10): 533-536.
7. 袁立燕, 谢杰, 薛汝增, 等. 白细胞介素37的免疫学研究. 皮肤性病诊疗学杂志, 2016, 23(4): 280-284.
8. Okamura H, Tsutsi H, Komatsu T, et al. Cloning of a new cytokine that induces IFN-γ production by T cells. Nature, 1995, 378(6552): 88.
9. Biet F, Locht C, Kremer L. Immunoregulatory functions of interleukin 18 and its role in defense against bacterial pathogens. J Mol Med, 2002, 80(3): 147.
10. Nold MF, Nold-Petry CA, Zepp JA, et al. IL-37 is a fundamental inhibitor of innate immunity. Nat Immunol, 2010, 11(11): 1014-1022.
11. Sakai N, Van Sweringen HL, Belizaire RM, et al. Interleukin-37 reduces liver inflammatory injury via effects on hepatocytes and non-parenchymal cells. J Gastroenterol Hepatol, 2012, 27: 1609-1616.
12. Boraschi D, Lucchesi D, Hainzl S, et al. IL-37: a new anti-inflammatory cytokine of the IL-1 family. Eur Cytokine Netw, 2011, 22(3): 127-147.
13. 许桂芳, 彭勇, 陈勇. 痛风患者血清中IL-37、IL-1β及IL-18水平检测及其相关性研究. 温州医科大学学报, 2014, 44(5): 370-373.
14. Zhao PW, Jiang WG, Wang L, et al. Plasma levels of IL-37 and correlation with TNF-α, IL-17A, and disease activity during DMARD treatment of rheumatoid arthritis. PLoS One, 2014, 9(5): e95346.
15. Imaeda H, Takahashi K, Fujimoto T, et al. Epithelial expression of interleukin-37b in inflammatory bowel disease. Clin Exp Immunol, 2013, 172: 410-416.
16. Bufler P, Gamboni-Robertson F, Azam T, et al. Interleukin-1 homologues IL-1F7b and IL-18 contain functional mRNA instability elements within the coding region responsive to lipopolysaccharide. Biochem J, 2004, 381(Pt 2): 503-510.
17. Hayashi N, Yoshimoto T, Izuhara K, et al. T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-gamma and IL-13 production. Proc Natl Acad Sci USA, 2007, 104(37): 14765-14770.
18. 吕盛秋, 李超乾. 白细胞介素-18在支气管哮喘中的研究进展. 免疫学杂志, 2014, 30(4): 347-350.
19. Li HP, Li X, He GJ, et al. The influence of dexamethasone on the proliferation and apoptosis of pulmonary inflammatory cells in bleomycin-induced pulmonary fibrosis in rats. Respirology, 2004, 9: 25-32.
20. Chen F, Gong L, Zhang L, et al. Short courses of low dose dexamethasone delay bleomycin-induced lung fibrosis in rats. Eur J Pharmacol, 2006, 536: 287-295.

Chinese Journal of Respiratory and Critical Care Medicine

The role and significance of interleukin-37 and related factors in the model of acute lung injury of rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content