The expression of programmed cell death-1 in peripheral blood of patients with asthma_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZHANG Chenying , WU Xingping , LI Chunhua ,  LI Jiashu

Department of Respiratory Medicine, First People’s Hospital of Lianyungang Affiliated to Xuzhou Medical University, Lianyungang, Jiangsu 222002, P. R. China;

Corresponding author：

LI Jiashu, Email: lissm1118@sina.com

Keywords：

Asthma; Programmed cell death-1; Interferon-γ; Interleukin-4; T help cells

DOI：

10.7507/1671-6205.201706008

Video：

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Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo investigate the expression of programmed cell death-1 (PD-1), interferon-γ (IFN-γ), interleukin-4 (IL-4) and T help cells (Th) in peripheral blood of patients with asthma.MethodsForty-one asthma patients were divided into an acute group (20 cases) and a chronic group (21 cases) according to the disease state of asthma. Another 18 healthy subjects were recruited as a control group. Peripheral blood samples were collected from all the subjects at admission or inclusion. The concentrations of PD-1, IFN-γ and IL-4 were detected by enzyme-linked immunoassay method. The expressions of Th1 and Th2 were detected by flow cytometry method.ResultsThe PD-1 and IFN-γ concentrations, Th1 proportion and Th1/Th2 ratio in the two asthma groups were reduced compared with the healthy control group, and were significantly lower in the acute group compared with the chronic group (all P<0.05). The IL-4 level and Th2 proportion in the two asthma groups were increased compared with the healthy control group, and were significantly higher in the acute group than the chronic group (allP<0.05). In the acute group, the PD-1 level was positively correlated with IFN-γ and Th1 level (r values were 0.678 and 0.712, respectively), and negatively correlated with IL-4 and Th2 (r values were –0.745 and –0.700, respectively).ConclusionThe concentration of PD-1 in patients with asthma is reduced especially in acute asthma, and shows close correlation with conventional index of asthma.

Citation： ZHANG Chenying, WU Xingping, LI Chunhua, LI Jiashu. The expression of programmed cell death-1 in peripheral blood of patients with asthma. Chinese Journal of Respiratory and Critical Care Medicine, 2018, 17(4): 390-393. doi: 10.7507/1671-6205.201706008 Copy

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13.	Iwanmura C, Shinoda K, Endo Y, et al. Regulation of memory CD4 T-cell pool size and function by natural killer T cells in vivo. Proc Natl Acad Sci USA, 2012, 109(42): 16992-16997.
14.	Saenz SA, Noti M, Artis D. Innate immune cell populations function as initiators and effectors in Th2 cytokine responses. Trends Immunol, 2010, 31(11): 407-413.
15.	Alonso MN, Wong MT, Zhang AL, et al. TH1, TH2, and TH17 cell instruct monocytes to differentiate into specialized dendritic cell subsets. Blood, 2011, 118(12): 3311-3320.
16.	Maldonado RA, Soriano MA, Perdomo LC, et al. Control of T helper cell differentiation through cytokine receptor inclusion in the immunological synapse. J Exp Med, 2009, 206(4): 877-892.
17.	Kim Y, Lee S, Kim YS, et al. Regulation of Th1/Th2 cells in asthma development: a mathematical model . Math Biosci Eng, 2013, 10(4): 1095-1133.
18.	Taube JM, Klein AP, Brahmer JR, et al. Association of PD-1, PD-1 ligands, and other features of the tumor immune microenvironment with response to anti–PD-1 therapy. Clin Cancer Res, 2014, 20(19): 5064-5074.
19.	Huang RY, Eppolito C, Lele S, et al. LAG3 and PD1co-inhibitory molecules collaborate to limit CD8⁺ T cellsignaling and dampen antitumor immunity in a murine ovarian cancer model. Oncotarget, 2015, 6(29): 27359-27377.
20.	Nguyen LT, Ohashi PS. Clinical blockade of PD1 and LAG3--potential mechanisms of action. Nat Rev Immunol, 2015, 15(1): 45-56.

1. Bateman ED, Hurd SS, Barnes PJ, et al. Global strategy for asthma management and prevention: GINA executive summary. Eur Respir J, 2008, 31(1): 143-178.
2. Song WJ, Kang MG, Chang YS, et al. Epidemiology of adult asthma in Asia : toward a better understanding. Asia Pac Allergy, 2014, 4(2): 75-85.
3. Jose BP, Camargos PA, Cruz Filho AA, et al. Diagnostic accuracy of respiratory diseases in primary health units. Rev Assoc Med Bras, 2014, 60(6): 599-612.
4. 中华医学会呼吸病学分会哮喘学组. 支气管哮喘防治指南(2016 版). 中华结核和呼吸杂志, 2016, 39(9): 1-21.
5. 陶国华, 曹兴建. 流式细胞术检测 Th1/Th2 的方法探讨. 临床检验杂志, 2006, 24(2): 116-117.
6. Fife BT, Pauken KE. The role of the PD-1 pathway in autoimmunity and peripheral tolerance. Ann N Y Acad Sci, 2011, 1217: 45-59.
7. Pita-López ML, Pera A, Solana R. Adaptive memory of human NK-like CD8+ T-cells to aging, and viral and tumor antigens. Front Immunol, 2016, 7: 616.
8. Jutz S, Leitner J, Schmetterer K, et al. Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: simultaneous measurement of NF-κB, NFAT and AP-1. J Immunol Methods, 2016, 430: 10-20.
9. Parry RV, Chemnitz JM, Frauwirth KA, et al. CTLA-4 and PD-1 receptors inhibit T cell activation by distinct mechanisms. Mol Cell Biol, 2005, 25(21): 9543-9553.
10. Zamani MR, Aslani S, Salmaninejad A, et al. PD-1/PD-L and autoimmunity: A growing relationship. Cell Immunol, 2016, 310: 27-41.
11. Lee SK, Rrgby RJ, Zotos D, et al. B cell priming for extrafollicular antibody responses requires Bcl-6 expression by T cells. J Exp Med, 2011, 208(7): 1377-1388.
12. Keir ME, Butte MJ, Freeman GJ, et al. PD-1 and its ligands in tolerance and immunity. Annu Rev Immunol, 2008, 26: 677-704.
13. Iwanmura C, Shinoda K, Endo Y, et al. Regulation of memory CD4 T-cell pool size and function by natural killer T cells in vivo. Proc Natl Acad Sci USA, 2012, 109(42): 16992-16997.
14. Saenz SA, Noti M, Artis D. Innate immune cell populations function as initiators and effectors in Th2 cytokine responses. Trends Immunol, 2010, 31(11): 407-413.
15. Alonso MN, Wong MT, Zhang AL, et al. TH1, TH2, and TH17 cell instruct monocytes to differentiate into specialized dendritic cell subsets. Blood, 2011, 118(12): 3311-3320.
16. Maldonado RA, Soriano MA, Perdomo LC, et al. Control of T helper cell differentiation through cytokine receptor inclusion in the immunological synapse. J Exp Med, 2009, 206(4): 877-892.
17. Kim Y, Lee S, Kim YS, et al. Regulation of Th1/Th2 cells in asthma development: a mathematical model . Math Biosci Eng, 2013, 10(4): 1095-1133.
18. Taube JM, Klein AP, Brahmer JR, et al. Association of PD-1, PD-1 ligands, and other features of the tumor immune microenvironment with response to anti–PD-1 therapy. Clin Cancer Res, 2014, 20(19): 5064-5074.
19. Huang RY, Eppolito C, Lele S, et al. LAG3 and PD1co-inhibitory molecules collaborate to limit CD8⁺ T cellsignaling and dampen antitumor immunity in a murine ovarian cancer model. Oncotarget, 2015, 6(29): 27359-27377.
20. Nguyen LT, Ohashi PS. Clinical blockade of PD1 and LAG3--potential mechanisms of action. Nat Rev Immunol, 2015, 15(1): 45-56.

Chinese Journal of Respiratory and Critical Care Medicine

The expression of programmed cell death-1 in peripheral blood of patients with asthma

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