The protective effects of rosiglitazone in chronic obstructive pulmonary disease rat models_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZHONG Liandi ¹ , ZHANG Chunlai ¹ , ZUO Wanli ¹ , ZHOU Wei ² , HUANG Shengqi ³ , ZHANG Xin ^2,4 ,  HUANG Yanming ^1,4

1. Department of Respiration Medicine, Jiangmen Central Hospital, Jiangmen, Guangdong 529030, P. R. China;
2. Department of Pathology, Jiangmen Central Hospital, Jiangmen, Guangdong 529030, P. R. China;
3. Department of Clinical Laboratory, Jiangmen Central Hospital, Jiangmen, Guangdong 529030, P. R. China;
4. Clinical Experimental Center, Jiangmen Central Hospital, Jiangmen, Guangdong 529030, P. R. China;

Corresponding author：

HUANG Yanming, Email: huangyanming_jxy@163.com

Keywords：

Chronic obstructive pulmonary disease; Rosiglitazone; Airway inflammation

DOI：

10.7507/1671-6205.201708052

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo investigate the protecting effect of rosiglitazone for lung in airway-instillation- lipopolysaccharides and smoke-induced chronic obstructive pulmonary disease (COPD) rat models.MethodsFifty male Wistar rats with the SPF standard were randomly divided into 5 groups (n=10). The rats were treated by airway-instillation-lipopolysaccharides and exposing to smoking to establish COPD rat models excepted normal group, and the treatment groups were received gavage rosiglitazone of 0.1 mg/kg, 0.15 mg/kg, and 0.2 mg/kg rosiglitazone daily for 30 days, and the normal group or model group was received gavage normal saline. All rats were sacrificed after 30 days' treatment, and the lung tissue section was stained by hematoxylin and eosin. The mean linear intercept (MLI) and mean alveolar numbers (MAN) were measured in all groups. In addition, the protein levels of p-Stat3 and p-NF-κB were detected by immunohistochemistry.ResultsCompared with normal group, the inflammation and emphysema were observed in the lung of rats in model group, and the symptoms of the group treated with rosiglitazone were lighter than normal group. The lungs of rats treated with highest dose of rosiglitazone (0.2 mg/kg) were evaluated with lowest pathology assessment score among three treatment groups, but there was no significant difference of MLI or MAN among three treatment groups. Compared with normal group, the protein levels of p-Stat3 and p-NF-κB were increased in the lung and tracheal epithelium and lymphoid tissue of rats in model group, while the protein levels of p-NF-κB were decreased in these tissues and the protein levels of p-Stat3 were decreased in the lymphoid tissue after treatment with rosiglitazone, but the protein levels of p-Stat3 were not changed in the lung and tracheal epithelium.ConclusionRosiglitazone has a protective effect on the COPD rat models by inhibiting NF-κB pathway to reduce the inflammation of the lung parenchyma.

Citation： ZHONG Liandi, ZHANG Chunlai, ZUO Wanli, ZHOU Wei, HUANG Shengqi, ZHANG Xin, HUANG Yanming. The protective effects of rosiglitazone in chronic obstructive pulmonary disease rat models. Chinese Journal of Respiratory and Critical Care Medicine, 2018, 17(3): 230-236. doi: 10.7507/1671-6205.201708052 Copy

1.	Decramer M, Janssens W, Miravitlles M. Chronic obstructive pulmonary disease. Lancet, 2012, 379(9823): 1341-1351.
2.	Zaher C, Halbert R, Dubois R, et al. Smoking-related diseases: the importance of COPD. Int J Tuberc Lung Dis, 2004, 8(12): 1423-1428.
3.	Belvisi M G, Hele D J. Peroxisome proliferator-activated receptors as novel targets in lung disease. Chest, 2008, 134(1): 152-157.
4.	Kadmiel M, Cidlowski J A. Glucocorticoid receptor signaling in health and disease. Trends Pharmacol Sci, 2013, 34(9): 518-530.
5.	胡蓉, 严国锋, 姜虹, 等. 改良吸烟复合气道内注入 LPS 法 COPD 大鼠模型的建立. 实验动物与比较医学, 2009, 29(3): 153-156.
6.	夏熙郑, 巨春蓉. Smads 蛋白和转化生长因子 β1 在大鼠慢性阻塞性肺疾病中的表达. 中国呼吸与危重监护杂志, 2006, 5(3): 193-197+245.
7.	金志贤, 王清, 郭翔, 等. 间充质干细胞移植肺气肿大鼠肺组织病理改变及炎性因子表达变化. 山东医药, 2015, 55(15): 20-22.
8.	谭焰, 季磊, 谷伟, 等. 噻托溴铵对慢性阻塞性肺疾病大鼠膈肌纤维的影响. 中国呼吸与危重监护杂志, 2014, 13(4): 344-347.
9.	张凌红, 段雅乐, 李于博, 等. 罗格列酮在大鼠体内药动学及组织分布研究. 华东师范大学学报(自然科学版), 2011, 2011(4): 104-114.
10.	李穆琼, 朱星枚, 李晓晔, 等. 罗格列酮钠片在健康人体的生物等效性研究. 药物分析杂志, 2009, 29(12): 2014-2017.
11.	张春来, 黄炎明, 钟莲娣. 罗格列酮对慢性阻塞性肺疾病大鼠的抗炎作用. 广东医学, 2017, 38(14): 2119-2121.
12.	Chung SW, Kang BY, Kim SH, et al. Oxidized low density lipoprotein inhibits interleukin-12 production in lipopolysaccharide-activated mouse macrophages via direct interactions between peroxisome proliferator-activated receptor-gamma and nuclear factor-kappa B. J Biol Chem, 2000, 275(42): 32681-32687.
13.	Straus D S, Glass C K. Anti-inflammatory actions of PPAR ligands: new insights on cellular and molecular mechanisms. Trends Immunol, 2007, 28(12): 551-558.
14.	Das L M, Rosenjack J, Au L, et al. Hyper-inflammation and skin destruction mediated by rosiglitazone activation of macrophages in IL-6 deficiency. J Invest Dermatol, 2015, 135(2): 389-399.
15.	Lakshmi S P, Reddy A T, Zhang Y, et al. Down-regulated peroxisome proliferator-activated receptor gamma (PPARgamma) in lung epithelial cells promotes a PPARgamma agonist-reversible proinflammatory phenotype in chronic obstructive pulmonary disease (COPD). J Biol Chem, 2014, 289(10): 6383-6393.
16.	Zepp J A, Zacharias W J, Frank D B, et al. Distinct Mesenchymal Lineages and Niches Promote Epithelial Self-Renewal and Myofibrogenesis in the Lung. Cell, 2017, 170(6): 1134-1148.

1. Decramer M, Janssens W, Miravitlles M. Chronic obstructive pulmonary disease. Lancet, 2012, 379(9823): 1341-1351.
2. Zaher C, Halbert R, Dubois R, et al. Smoking-related diseases: the importance of COPD. Int J Tuberc Lung Dis, 2004, 8(12): 1423-1428.
3. Belvisi M G, Hele D J. Peroxisome proliferator-activated receptors as novel targets in lung disease. Chest, 2008, 134(1): 152-157.
4. Kadmiel M, Cidlowski J A. Glucocorticoid receptor signaling in health and disease. Trends Pharmacol Sci, 2013, 34(9): 518-530.
5. 胡蓉, 严国锋, 姜虹, 等. 改良吸烟复合气道内注入 LPS 法 COPD 大鼠模型的建立. 实验动物与比较医学, 2009, 29(3): 153-156.
6. 夏熙郑, 巨春蓉. Smads 蛋白和转化生长因子 β1 在大鼠慢性阻塞性肺疾病中的表达. 中国呼吸与危重监护杂志, 2006, 5(3): 193-197+245.
7. 金志贤, 王清, 郭翔, 等. 间充质干细胞移植肺气肿大鼠肺组织病理改变及炎性因子表达变化. 山东医药, 2015, 55(15): 20-22.
8. 谭焰, 季磊, 谷伟, 等. 噻托溴铵对慢性阻塞性肺疾病大鼠膈肌纤维的影响. 中国呼吸与危重监护杂志, 2014, 13(4): 344-347.
9. 张凌红, 段雅乐, 李于博, 等. 罗格列酮在大鼠体内药动学及组织分布研究. 华东师范大学学报(自然科学版), 2011, 2011(4): 104-114.
10. 李穆琼, 朱星枚, 李晓晔, 等. 罗格列酮钠片在健康人体的生物等效性研究. 药物分析杂志, 2009, 29(12): 2014-2017.
11. 张春来, 黄炎明, 钟莲娣. 罗格列酮对慢性阻塞性肺疾病大鼠的抗炎作用. 广东医学, 2017, 38(14): 2119-2121.
12. Chung SW, Kang BY, Kim SH, et al. Oxidized low density lipoprotein inhibits interleukin-12 production in lipopolysaccharide-activated mouse macrophages via direct interactions between peroxisome proliferator-activated receptor-gamma and nuclear factor-kappa B. J Biol Chem, 2000, 275(42): 32681-32687.
13. Straus D S, Glass C K. Anti-inflammatory actions of PPAR ligands: new insights on cellular and molecular mechanisms. Trends Immunol, 2007, 28(12): 551-558.
14. Das L M, Rosenjack J, Au L, et al. Hyper-inflammation and skin destruction mediated by rosiglitazone activation of macrophages in IL-6 deficiency. J Invest Dermatol, 2015, 135(2): 389-399.
15. Lakshmi S P, Reddy A T, Zhang Y, et al. Down-regulated peroxisome proliferator-activated receptor gamma (PPARgamma) in lung epithelial cells promotes a PPARgamma agonist-reversible proinflammatory phenotype in chronic obstructive pulmonary disease (COPD). J Biol Chem, 2014, 289(10): 6383-6393.
16. Zepp J A, Zacharias W J, Frank D B, et al. Distinct Mesenchymal Lineages and Niches Promote Epithelial Self-Renewal and Myofibrogenesis in the Lung. Cell, 2017, 170(6): 1134-1148.

Chinese Journal of Respiratory and Critical Care Medicine

The protective effects of rosiglitazone in chronic obstructive pulmonary disease rat models

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content