慢性阻塞性肺疾病患者 T 细胞亚群变化及 CD8<sup>+</sup>CD28<sup>+</sup> T 细胞与 C 反应蛋白相关性探讨_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

郝月琴 ¹ , 王欣 ² , 李猛 ¹ , 陈建攸 ¹ ,  唐华平 ¹

1. ;
2. ;

Corresponding author：

唐华平, Email: qdthp@126.com

Keywords：

慢性阻塞性肺疾病; 急性加重; T 细胞亚群; 杀伤性 T 细胞; C 反应蛋白

DOI：

10.7507/1671-6205.201812018

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的探讨慢性阻塞性肺疾病（简称慢阻肺）急性加重期治疗前后外周血 T 细胞亚群变化、CD8⁺CD28⁺ T 细胞与 C 反应蛋白（CRP）的相关性及其临床意义。方法选择 2015 年 5 月至 2017 年 10 月青岛市市立医院呼吸科慢阻肺急性加重期住院患者 30 例，治疗前及治疗后 2 周、治疗后 3 个月时均空腹抽血 3 mL 进行流式细胞分析 CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺、CD8⁺CD28⁺ T 细胞，并常规检测血清 CRP 水平。结果慢阻肺急性加重期患者细胞免疫功能低下，CD4⁺/CD8⁺比值下降，在治疗后 CD3⁺、CD4⁺百分比及 CD4⁺/CD8⁺比值均逐渐升高，但治疗前与治疗后 2 周对比差异无统计学意义，治疗前与治疗后 3 个月后对比有显著差异（P<0.05）。治疗前与治疗后对比 CD8⁺CD28⁺ T 细胞及 CRP 均显著下降（P<0.05），且二者呈正相关（r=0.86，P<0.05）。结论慢阻肺急性加重患者细胞免疫功能低下紊乱，患者体内 T 细胞亚群失衡的恢复是一个慢性过程。CD8⁺CD28⁺ T 细胞及 CRP 可作为判断慢阻肺患者病情严重程度和预后的指标。

Citation： 郝月琴, 王欣, 李猛, 陈建攸, 唐华平. 慢性阻塞性肺疾病患者 T 细胞亚群变化及 CD8⁺CD28⁺ T 细胞与 C 反应蛋白相关性探讨. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(1): 75-77. doi: 10.7507/1671-6205.201812018 Copy

1.	Zhang MQ, Wan Y, Jin Y, et al. Cigarette smoking promotes inflammation in patients with COPD by affecting the polarization and survival of Th/Tregs through up-regulation of muscarinic receptor 3 and 5 expression. PLoS One, 2014, 9(11): e112350.
2.	Zhou H, Hua W, Jin Y, et al. Tc17 cells are associated with cigarette smoke-induced lung inflammation and emphysema. Respirology, 2015, 20(3): 426-433.
3.	Podolin PL, Foley JP, Carpenter DC, et al. T cell depletion protects against alveolar destruction due to chronic cigarette smoke exposure in mice. Am J Physiol Lung Cell Mol Physiol, 2013, 304(5): L312-L323.
4.	陈宁, 徐燕, 张永红, 等. 慢性阻塞性肺病大鼠外周血中 Foxp3⁺调节性 T 细胞及 IL-17、IL-6 水平的变化. 中国现代医学杂志, 2014, 24(27): 24-28.
5.	Mathai RT, Bhat S. Peripheral blood T-cell populations in COPD, asymptomatic smokers and healthy non-smokers in Indian subpopulation-a pilot study. J Clin Diagn Res, 2013, 7(6): 1109-1113.
6.	李思聪, 卓宋明. T 淋巴细胞免疫在慢性阻塞性肺疾病急性发作中的影响及相关免疫干预进展. 临床肺科杂志, 2015, 20(10): 1898-1901.
7.	王文娟, 刘淑红, 姜玉荣, 等. COPD 病患者免疫功能的变化. 临床肺科杂志, 2012, 17(8): 1511-1512.
8.	Freeman CM, Martinez CH, Todt JC, et al. Acute exacerbations of chronic obstructive pulmonary disease are associated with decreased CD4+ & CD8+ T cells and increased growth & differentiation factor-15 (GDF-15) in peripheral blood. Respir Res, 2015, 16: 94.
9.	Hou J, Sun Y, Hao Y, et al. Imbalance between subpopulations of regulatory T cells in COPD. Thorax, 2013, 68(12): 1131-1139.
10.	Forsslund H, Mikko M, Karimi R, et al. Distribution of T-Cell subsets in BAL fluid of patients with mild to moderate COPD depends on current smoking status and not airway obstruction. Chest, 2014, 145(4): 711-722.
11.	Freeman CM, McCubbrey AL, Crudgington S, et al. Basal gene expression by lung CD4+ T cells in chronic obstructive pulmonary disease identifies independent molecular correlates of airflow obstruction and emphysema extent. PLoS One, 2014, 9(5): e96421.
12.	Chen L, Chen G, Zhang MQ, et al. Imbalance between subsets of CD8+ peripheral blood T cells in patients with chronic obstructive pulmonary disease. Peer J, 2016, 4: e230.
13.	Hodge G, Mukaro V, Reynolds PN, et al. Role of increased CD8/CD28^null T cells and alternative co-stimulatory molecules in chronic obstructive pulmonary disease. Clin Exp Immunol, 2011, 166(1): 94-102.
14.	江雨顺, 刘达富. 慢性阻塞性肺疾病患者外周血 T 淋巴细胞亚群和 C 反应蛋白变化的临床意义. 白求恩医学杂志, 2016, 14(5): 580-582.

1. Zhang MQ, Wan Y, Jin Y, et al. Cigarette smoking promotes inflammation in patients with COPD by affecting the polarization and survival of Th/Tregs through up-regulation of muscarinic receptor 3 and 5 expression. PLoS One, 2014, 9(11): e112350.
2. Zhou H, Hua W, Jin Y, et al. Tc17 cells are associated with cigarette smoke-induced lung inflammation and emphysema. Respirology, 2015, 20(3): 426-433.
3. Podolin PL, Foley JP, Carpenter DC, et al. T cell depletion protects against alveolar destruction due to chronic cigarette smoke exposure in mice. Am J Physiol Lung Cell Mol Physiol, 2013, 304(5): L312-L323.
4. 陈宁, 徐燕, 张永红, 等. 慢性阻塞性肺病大鼠外周血中 Foxp3⁺调节性 T 细胞及 IL-17、IL-6 水平的变化. 中国现代医学杂志, 2014, 24(27): 24-28.
5. Mathai RT, Bhat S. Peripheral blood T-cell populations in COPD, asymptomatic smokers and healthy non-smokers in Indian subpopulation-a pilot study. J Clin Diagn Res, 2013, 7(6): 1109-1113.
6. 李思聪, 卓宋明. T 淋巴细胞免疫在慢性阻塞性肺疾病急性发作中的影响及相关免疫干预进展. 临床肺科杂志, 2015, 20(10): 1898-1901.
7. 王文娟, 刘淑红, 姜玉荣, 等. COPD 病患者免疫功能的变化. 临床肺科杂志, 2012, 17(8): 1511-1512.
8. Freeman CM, Martinez CH, Todt JC, et al. Acute exacerbations of chronic obstructive pulmonary disease are associated with decreased CD4+ & CD8+ T cells and increased growth & differentiation factor-15 (GDF-15) in peripheral blood. Respir Res, 2015, 16: 94.
9. Hou J, Sun Y, Hao Y, et al. Imbalance between subpopulations of regulatory T cells in COPD. Thorax, 2013, 68(12): 1131-1139.
10. Forsslund H, Mikko M, Karimi R, et al. Distribution of T-Cell subsets in BAL fluid of patients with mild to moderate COPD depends on current smoking status and not airway obstruction. Chest, 2014, 145(4): 711-722.
11. Freeman CM, McCubbrey AL, Crudgington S, et al. Basal gene expression by lung CD4+ T cells in chronic obstructive pulmonary disease identifies independent molecular correlates of airflow obstruction and emphysema extent. PLoS One, 2014, 9(5): e96421.
12. Chen L, Chen G, Zhang MQ, et al. Imbalance between subsets of CD8+ peripheral blood T cells in patients with chronic obstructive pulmonary disease. Peer J, 2016, 4: e230.
13. Hodge G, Mukaro V, Reynolds PN, et al. Role of increased CD8/CD28^null T cells and alternative co-stimulatory molecules in chronic obstructive pulmonary disease. Clin Exp Immunol, 2011, 166(1): 94-102.
14. 江雨顺, 刘达富. 慢性阻塞性肺疾病患者外周血 T 淋巴细胞亚群和 C 反应蛋白变化的临床意义. 白求恩医学杂志, 2016, 14(5): 580-582.

Previous Article
肉芽肿性多血管炎致中枢性尿崩症一例并文献复习
Next Article
重症加强治疗病房危重患者俯卧位通气垫的研制与使用

Chinese Journal of Respiratory and Critical Care Medicine

慢性阻塞性肺疾病患者 T 细胞亚群变化及 CD8⁺CD28⁺ T 细胞与 C 反应蛋白相关性探讨

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content

Chinese Journal of Respiratory and Critical Care Medicine

慢性阻塞性肺疾病患者 T 细胞亚群变化及 CD8+CD28+ T 细胞与 C 反应蛋白相关性探讨

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content

慢性阻塞性肺疾病患者 T 细胞亚群变化及 CD8⁺CD28⁺ T 细胞与 C 反应蛋白相关性探讨